start-ver=1.4 cd-journal=joma no-vol=80 cd-vols= no-issue=1 article-no= start-page=17 end-page=24 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Activation of β-Glucosidase and Accumulation of Secondary Metabolites in Pea SeedlingsTreated with a Biotic-elicitor Chitosan kn-title=生物的エリシター・キトサンで処理したエンドウにおけるβ-グルコシダーゼの活性化と二次代謝産物誘導 en-subtitle= kn-subtitle= en-abstract= kn-abstract=生物的エリシター・キトサンで処理したエンドウ上胚軸におけるβ-グルコシダーゼの活性は,72時間後には処理前の約60倍に達した.塩化第二銅処理したエンドウ実生から2つのkaempferol-β-glucoside,quercetin-β-glucoside,およびエンドウのファイトアレキシンであるピサチンを精製・単離した.ピサチンはキトサン処理72時間後に最大に達した.kaempferol配糖体はキトサン処理24時間後に最大に達し,48時間から72時間の間に消失した.quercetin配糖体はキトサン処理24時間後に最大に達し,86時間後にはコントロールと同じレベルまで減少した.kaempferolとquercetinはピサチン生合成系における前駆体ではないので,これらは細胞壁の強化に利用されていると考えられる。 en-copyright= kn-copyright= en-aut-name=KobayashiAkio en-aut-sei=Kobayashi en-aut-mei=Akio kn-aut-name=小林昭雄 kn-aut-sei=小林 kn-aut-mei=昭雄 aut-affil-num=1 ORCID= en-aut-name=AkiyamaKohki en-aut-sei=Akiyama en-aut-mei=Kohki kn-aut-name=秋山康紀 kn-aut-sei=秋山 kn-aut-mei=康紀 aut-affil-num=2 ORCID= en-aut-name=UshijimaTamano en-aut-sei=Ushijima en-aut-mei=Tamano kn-aut-name=牛嶋珠乃 kn-aut-sei=牛嶋 kn-aut-mei=珠乃 aut-affil-num=3 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=4 ORCID= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=80 cd-vols= no-issue=1 article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A Compound Which Can Be Used for Selecting Transfomed Plant Cells kn-title=植物形質転換細胞の選抜機能を有する化合物 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Streptomyces sp,KBFP-2025株が生産するOxazolomycinは,Agrobacterium tumefaciensに対する特異的な抗菌活性により,ジャガイモのクラウンゴール形成を阻害する.さらに,本化合物はアルファルファの発芽を強く阻害し,ジャガイモ塊茎細胞を壊死させたが,ジャガイモのタラウンゴール細胞に対する生育阻害を示さなかった.すなわち,本化合物は非形質転換植物細胞に対する選択毒性を有しており,形質転換細胞の選抜に有用である。 en-copyright= kn-copyright= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=1 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=2 ORCID= en-aut-name=KwaiSatoru en-aut-sei=Kwai en-aut-mei=Satoru kn-aut-name=川井悟 kn-aut-sei=川井 kn-aut-mei=悟 aut-affil-num=3 ORCID= en-aut-name=KobayashiAkio en-aut-sei=Kobayashi en-aut-mei=Akio kn-aut-name=小林昭雄 kn-aut-sei=小林 kn-aut-mei=昭雄 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=1 article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19960201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=土壌から単離した真菌の培養物についての殺線虫活性のスクリーニングと殺線虫活性のある1菌株培養上清の予備分画 kn-title=Screening of Fungal Cultures for Nematicidal Activity and Preliminary Fractionation of a Nematicidal Fungal Culture en-subtitle= kn-subtitle= en-abstract=土壌から単離した99菌株の真菌の培養物について、以前の著者の1人が開発した線虫増殖阻害活性を検出するバイオアッセイを用いてスクリーニングを行った。1菌株の遠心分離上清に活性を認めた。予備的に行った各種クロマトグラフィーにおける挙動から、本活性化合物は、水溶性の中性化合物であると推定した。 kn-abstract=Cultures of 99 strains of fungi in potato-sucrose-malt extract medium were screened for nematicidal activity. Strong activity was found in the supernatant of the culture of fungus 18. Activity-guided preliminary fractionation of the supernatant showed that the active compound was neutral and water soluble. en-copyright= kn-copyright= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=1 ORCID= en-aut-name=SunJian Hua en-aut-sei=Sun en-aut-mei=Jian Hua kn-aut-name=孫建華 kn-aut-sei=孫 kn-aut-mei=建華 aut-affil-num=2 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=nematicidal activity kn-keyword=nematicidal activity en-keyword=screening of fungal cultures kn-keyword=screening of fungal cultures en-keyword=Bursaphelenchus xylophilus kn-keyword=Bursaphelenchus xylophilus END start-ver=1.4 cd-journal=joma no-vol=86 cd-vols= no-issue=1 article-no= start-page=1 end-page=5 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=199702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=細胞毒性化合物、DeoxypodophyllotoxinのJuniperus chinensis 茎葉からの単離と同定 kn-title=Isolation of the Cytotoxic Constituent Deoxypodophyllotoxin from the Leaves of Juniperus chinensis en-subtitle= kn-subtitle= en-abstract=マレーシアなど熱帯地方において、庭木としてよく植栽されているJuniperus chinensisの茎葉のエタノール抽出物から　HeLa細胞に対する毒性を指標として、細胞毒性化合物Deoxypodophyllotoxinを単離した。この化合物のHeLa細胞に対するMECは、0.004μg/mlであった。 kn-abstract=Deoxypodophyllotoxin was isolated as a cytotoxic constituent from ethanol extract of the leaves of Juniperus chinensis by assay-guided fractionation. en-copyright= kn-copyright= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=1 ORCID= en-aut-name=IsmailIntan S. en-aut-sei=Ismail en-aut-mei=Intan S. kn-aut-name=イスマイルインタン サフィナ kn-aut-sei=イスマイル kn-aut-mei=インタン サフィナ aut-affil-num=2 ORCID= en-aut-name=TakahataKyouya en-aut-sei=Takahata en-aut-mei=Kyouya kn-aut-name=高畑京也 kn-aut-sei=高畑 kn-aut-mei=京也 aut-affil-num=3 ORCID= en-aut-name=AliAbdul Manaf en-aut-sei=Ali en-aut-mei=Abdul Manaf kn-aut-name=アリアブドゥル マナフ kn-aut-sei=アリ kn-aut-mei=アブドゥル マナフ aut-affil-num=4 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=Faculty of Food Science and Biotechnology, Universiti Pertanian Malaysia affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=Faculty of Food Science and Biotechnology, Universiti Pertanian Malaysia affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=cytotoxic activity kn-keyword=cytotoxic activity en-keyword=HeLa cell kn-keyword=HeLa cell en-keyword=assay-guided fractionation kn-keyword=assay-guided fractionation en-keyword=Juniperus chinensis kn-keyword=Juniperus chinensis END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=9 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Tectona grandis の母植物体には少量しか、あるいは全然存在しない抗菌性トリテルペン酸の動植物細胞懸濁培養による生産 kn-title=Production of Antibacterial Triterpene Acids Not Detected in the Native Plant by Cell Suspension Culture of Tectona grandis en-subtitle= kn-subtitle= en-abstract=著者らは以前、Tectona grandis のカルスが、母植物には少量しか、あるいは全然存在しない抗菌性トリテルペン酸を相当量することを報告した。本報は、確立した細胞懸濁培養系では、これらの抗菌性トリテルペン酸の生産性がカルス培養の100～200倍にも上昇することを述べ、これらの抗菌性化合物を細胞懸濁培養で生産できることを示した。 kn-abstract=The callus culture of Tectona grandis was previouly reported by us to produce five antibacterial acids which occurred only in very small amounts or were not detected in the native plant. This paper shows the production of these antibacterial compounds in much higher by cell suspension culture of the plant. en-copyright= kn-copyright= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=1 ORCID= en-aut-name=MarwaniErly en-aut-sei=Marwani en-aut-mei=Erly kn-aut-name=マルワニアーリー kn-aut-sei=マルワニ kn-aut-mei=アーリー aut-affil-num=2 ORCID= en-aut-name=KobayasiAkiko en-aut-sei=Kobayasi en-aut-mei=Akiko kn-aut-name=小林昭雄 kn-aut-sei=小林 kn-aut-mei=昭雄 aut-affil-num=3 ORCID= en-aut-name=NitodaTeruhiko en-aut-sei=Nitoda en-aut-mei=Teruhiko kn-aut-name=仁戸田照彦 kn-aut-sei=仁戸田 kn-aut-mei=照彦 aut-affil-num=4 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=Department of Biotechnology, Faculty of Engineering, Osaka University affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=high productivity kn-keyword=high productivity en-keyword=callus culture kn-keyword=callus culture en-keyword=lupenoic acid kn-keyword=lupenoic acid en-keyword=oleanenoic acid kn-keyword=oleanenoic acid en-keyword=ursenoic acid kn-keyword=ursenoic acid END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=13 end-page=16 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=An Analytical Method of lnophyllums A,B,C,D,E,and P,Anti - HIV Constituents of Calophyllum inophyllum by HPLC kn-title=Calophyllum inophyllumの抗ＨＩＶ活性成分 inophyllum A, B, C, D, E, P の液体クロマトグラフィーによる分析法の確立 en-subtitle= kn-subtitle= en-abstract=最近、抗HIV活性化合物として注目されているCalophyllum inophyllumの葉の成分、inophyllum化合物を細胞培養で生産するためには、培養条件検討時に用いる当各成分の迅速かつ正確な分析法が必要である。本研究では、著者らの一人が以前に単離したinophyllum化合物のHPLC上の溶出位置を決定できることを示した。 kn-abstract=In order to optimize cell culture conditions for producing inhibitors of HIV reverse transcriptase isolated from Calophyllum , inophyllum , a rapid and accurate anaalytical method for determining the quantity of the inhibitors, inophyllums A , B , C , D , E , and P was desired . We have established a quantitative analytical method using HPLC ,together with LCMS . en-copyright= kn-copyright= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=1 ORCID= en-aut-name=NitodaTeruhiko en-aut-sei=Nitoda en-aut-mei=Teruhiko kn-aut-name=仁戸田照彦 kn-aut-sei=仁戸田 kn-aut-mei=照彦 aut-affil-num=2 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=LC-M3 kn-keyword=LC-M3 en-keyword=4-Phenylcoumarin kn-keyword=4-Phenylcoumarin en-keyword=Guttiferae kn-keyword=Guttiferae en-keyword=HIV reverse transcriptase kn-keyword=HIV reverse transcriptase en-keyword=AIDS kn-keyword=AIDS END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=43 end-page=46 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Stabilization of cyclo (Gly-Tyr)-hydrolyzing Enzyme by Synthetic Glycoside-bearing Polymer kn-title=Cyclo (Gly-Tyr)分解酵素の合成高分子配糖体による安定化 en-subtitle= kn-subtitle= en-abstract=集積培養法を用いて新たに土壌よりcyclo(Gly-Tyr)資化性菌を分離し、その中から、従来の資化性菌より菌体当たりの活性の高い菌株を13株見いだした。これらの菌株を牛血清アルブミンを添加した緩衝液中に懸濁し、超音波破砕に供したところ、OI-L-2株由来の無細胞抽出液中にcyclo(Gly-Tyr)分解活性が検出された。この活性は安定化剤をアルブミンから配糖化合成高分子GEMAポリマーに代えることによりさらに安定に抽出された。 kn-abstract=An enrichment technique led us to isolate 13 cyclo (Gly-Tyr)-assimilating bacteria, which had higher cyclo (Gly-Tyr)-hydrolyzing activity that the coryneform rod bacterium strain T-1-3-Y,previously isolated from soil. The cell-free extract of the strain Ol-L-2 prepared by ultrasonication in a buffer containing bovine serum albumin had cyclo (Gly-Tyr)-hydrolyzing activity while that of the strain T-1-3-Y showed no activity. GEMA (Glucosyloxyethyl methacrylate) polymer was found to be a more effective stabilizer for solubilizing cyclo (Gly-Tyr) hydrolase than bovine serum albumin. en-copyright= kn-copyright= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=1 ORCID= en-aut-name=AbeYasuteru en-aut-sei=Abe en-aut-mei=Yasuteru kn-aut-name=安部泰輝 kn-aut-sei=安部 kn-aut-mei=泰輝 aut-affil-num=2 ORCID= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=diketopiperazine kn-keyword=diketopiperazine en-keyword=cyclic dipeptide kn-keyword=cyclic dipeptide en-keyword=hydrolysis kn-keyword=hydrolysis en-keyword=protease kn-keyword=protease END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=1 end-page=7 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=マツノザイセンチュウ，Bursaphelenchus xylophilusに随伴する松萎凋性細菌の単離とその毒性代謝物質 en-subtitle= kn-subtitle= en-abstract=マツ材線虫病の初期症状である樹脂道エピセリウム細胞や周辺柔細胞の変性は、マツノザイセンチュウのその場への到着および増殖に先行して起こることから、マツノザイセンチュウに随伴する微生物が発病に関与しているのだはないかと推論し、本研究では、クロマツ培養細胞に対する毒性を指標にして強病原生マツノザイセンチュウから毒物質生産細菌を数株を単離したえ。さらに、この毒性代謝物質を単離し、フェニル酢酸と同定した。フェニル酢酸生産細菌は、普遍的に存在するが、フェニル酢酸生産性には、大きい差があることを確認した。このフェニル酢酸生産細菌のみを接種しても、アカマツ実生は萎凋しなかったが、弱病原性マツノザイセンチュウに保持されて接種すると、ザイセンチュウの病原性が強くなった。このかとから、フェニル酢酸生産細菌は、マツノザイセンチュウに運搬されて松樹体内を移動し病原毒素フェニル酢酸を生産するものと推論した。 kn-abstract=Based on the observation that ray parenchyma cell death took place prior to nematode population increase in pine wood,the authors suspected that any microorganisms carried by pathogenic nematodes would be involvedin the pathogenic process.The authors screened pathogenic nematode-accompanying microbes for their toxicity against cultured cells of Pinus thunbergiis and isolated and identified 3 toxic strains,Bacillus cereus HY-3,B.subtilis HY-16,and Bmegaterium HY-17 , whose toxic products were identified as phenylacertic acid.Inoculation of pine seedlings with the toxic bacterium alone did not cause the seedling to wilt , but inoculation of pine seedlings with the toxic bacterium carried by weakly pathogenic nematodes wilted the seedling as much as strongly pathogenic nematodes . These results suggested that phenyllacetic acid-producing bacteria could invade pine trees by accompanying pine wood nematodes , and produce phenylacetic acid , a toxic metabolite , in the wood. en-copyright= kn-copyright= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=1 ORCID= en-aut-name=YamasitaHideaki en-aut-sei=Yamasita en-aut-mei=Hideaki kn-aut-name=山下秀昭 kn-aut-sei=山下 kn-aut-mei=秀昭 aut-affil-num=2 ORCID= en-aut-name=KobayasiAkio en-aut-sei=Kobayasi en-aut-mei=Akio kn-aut-name=小林昭雄 kn-aut-sei=小林 kn-aut-mei=昭雄 aut-affil-num=3 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=テルモ株式会社技術開発部 affil-num=3 en-affil= kn-affil=大阪大学大学院工学研究科応用生物工学専攻 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=pathogenic bacteria kn-keyword=pathogenic bacteria en-keyword=phenylacetic acid producer kn-keyword=phenylacetic acid producer en-keyword=nematode-accompanying bacteria kn-keyword=nematode-accompanying bacteria en-keyword=fluorescence microscopic analysis kn-keyword=fluorescence microscopic analysis en-keyword=pine wilt disease kn-keyword=pine wilt disease END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=1 end-page=5 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Reisolation of the Pathogens from Wilted Red Pine Seedings Inoculated with the Bacterium-carrying Nematode, and the Cause of Difference in Pathogenicity among Pine Wood Nematode Isolates kn-title=病原体を接種して萎凋させたアカマツ実生からの病原体の再分離およびマツノザイセンチュウの分離系統間の病原性の差の原因 en-subtitle= kn-subtitle= en-abstract=著者らはすでに、マツ材線虫病の真の病原体はマツノザイセンチュウによって搬入されるフェニル酢酸生産細菌であることを提唱し、さらに無菌化した強病原性分離系統のマツノザイセンチュウAOKD-3を接種した無菌アカマツ実生は萎凋しないことを示すことによって、本萎凋病の真の病原体はフェニル酢酸生産細菌であることを立証した。本報では、無菌にしたOKD-3(AOKD-3)に、再びフェニル酢酸生産細菌を保持させてアカマツの無菌実生に接種すれば、萎凋した実生からセンチュウおよびフェニル酢酸生産細菌を再分離することによって、フェニル酢酸生産細菌を保持したマツノザイセンチュウの病原性をコッホ氏の四原則にのっとって確認した。一方、マツノザイセンチュウの弱病原性分離系統は、フェニル酢酸生産細菌を保持してもなお、強病原性分離系統に比べ、萎凋率が低いことうを明らかにした。したがって、萎凋すなわち、マツ材線虫病の発現には、フェニル酢酸生産細菌のマツノザイセンチュウへの随伴に加えて、マツノザイセンチュウの各分離系統の固有の性質、たとえば運動性（移動力）、増殖力などが大きく関与していることを強く示唆した。 kn-abstract=This paper shows that aseptic red pine seedings inoculated with the aseptic nematode (AOKD-3) carrying the bacterium (Bacillus cereus strain HY-3) and the bacterium (HY-3) do not. These findings indicate that the phenylacetic acid producers accompanying the nematode are the pathogen of the pine wilt, conforming to Koch's four principles. The fact that the weakly pathogenic isolate of nematode cannot so effectively wilt the sedding as the strongly pathogenic isolate, no matter how pathogenic a bacterium accompanies it, suggests that inherent characters such as mobility and propagation of the nematode are also important for thenematode to wilt the seedling. en-copyright= kn-copyright= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=1 ORCID= en-aut-name=KanekoNoboru en-aut-sei=Kaneko en-aut-mei=Noboru kn-aut-name=金子昇 kn-aut-sei=金子 kn-aut-mei=昇 aut-affil-num=2 ORCID= en-aut-name=HiraokaKyoko en-aut-sei=Hiraoka en-aut-mei=Kyoko kn-aut-name=平岡享子 kn-aut-sei=平岡 kn-aut-mei=享子 aut-affil-num=3 ORCID= en-aut-name=YamashitaHideki en-aut-sei=Yamashita en-aut-mei=Hideki kn-aut-name=山下秀昭 kn-aut-sei=山下 kn-aut-mei=秀昭 aut-affil-num=4 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Koch's principle kn-keyword=Koch's principle en-keyword=Bursaphelenchus xylophilus kn-keyword=Bursaphelenchus xylophilus en-keyword=Bacillus cereus kn-keyword=Bacillus cereus en-keyword=Bacillus megaterium kn-keyword=Bacillus megaterium en-keyword=pathogen of pine wilt kn-keyword=pathogen of pine wilt END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=7 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Novel Cyclic Dipeptide Dehydrogenase and Assay Method for Activity kn-title=新規の環状ジペプチド脱水素酵素とその活性測定法の確立 en-subtitle= kn-subtitle= en-abstract=アルボノルシン生産菌、Streptomyces sp. KO2388株の無細胞抽出液が、cyclo(L-Phe-L-Leu)からアルボノルシンへの脱水素反応を触媒することを明らかにした。アミノ酸残基のα,β-位での脱水素反応を酵素レベルで明らかにしたのはこの報告が初めてである。本反応の簡便な測定法として、基質cyclo(L-Phe-L-Leu)には認められない。アルボノルシンの特異的な317nmにおける紫外吸収の増加を測定する方法を考案した。本方法を用いて数種の水素受容体を試験したところ、フェナジンメトサルフェートが最も有効であると判った。 kn-abstract=The cell-free extract prepared from cells of an albonoursin-producing actinomycete Streptomyces sp. KO2388 was found to catalyze the dehydrogenation of cyclo (L-Phe-L-Leu) (CFL) to albonoursin. This is the first report for the dehydrogenation at the α,β-positions of amino acid residues. The simple method for determining the dehydrogenation activity was devised by measuring the increase in UV absorption of the reaction mixture at 317nm, λmax(ε25,400) of albonoursin, where CFL had no absorption. Phenazine methosulfate was the most active cofactor for the dehydrogenation among several hydrogen acceptors. en-copyright= kn-copyright= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=1 ORCID= en-aut-name=AkazawaKazumi en-aut-sei=Akazawa en-aut-mei=Kazumi kn-aut-name=赤澤和美 kn-aut-sei=赤澤 kn-aut-mei=和美 aut-affil-num=2 ORCID= en-aut-name=ImuraDaisuke en-aut-sei=Imura en-aut-mei=Daisuke kn-aut-name=井村大輔 kn-aut-sei=井村 kn-aut-mei=大輔 aut-affil-num=3 ORCID= en-aut-name=NitodaTeruhiko en-aut-sei=Nitoda en-aut-mei=Teruhiko kn-aut-name=仁戸田照彦 kn-aut-sei=仁戸田 kn-aut-mei=照彦 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=albonoursin kn-keyword=albonoursin en-keyword=cyclo(phe-Leu) kn-keyword=cyclo(phe-Leu) en-keyword=dehydrogenation kn-keyword=dehydrogenation en-keyword=phenazine methosulfate kn-keyword=phenazine methosulfate END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=13 end-page=17 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Isolation and Purification of Nocardamine from the Validamycin Producer kn-title=バリダマイシン生成菌培養液から抗細菌性抗生物質ノカルダミンの分離・精製 en-subtitle= kn-subtitle= en-abstract=Streptomyces hygroscopicus subsp. limoneus はバリダマイシン生成菌として知られているが、その抗カビ物質以外に抗細菌性抗生物質を生成することは知られていない。われわれはその微生物がバリダマイシン非生成条件において生成する抗細菌性抗生物質を見出そうと試みた。その結果、グラム陰性細菌に活性を示す抗生物質EO-3を得たのでこれを分離・精製してノカルダミンと同定した。本報告では本抗生物質の培養条件、抗菌スペクトル、精製および同定方法について述べる。尚、この研究は平成6年度から8年度までの3年間に亙る岡山大学学内特定研究『特殊環境生物の機能開発と物質生産への応用』を分担して行ったものである。 kn-abstract=An antibacterial active against P. mirabilis was isolated from the culture of Streptomyces hygroscopicus subsp. limoneus, validamycin producer. The antibiotic was found to be produced with a non-validamycin producing condition. The antibiotic was identified as nocardamine with the analytical data, IR, 1H-NMR and 13C-NMR spectra. en-copyright= kn-copyright= en-aut-name=HigashideEiji en-aut-sei=Higashide en-aut-mei=Eiji kn-aut-name=東出栄治 kn-aut-sei=東出 kn-aut-mei=栄治 aut-affil-num=1 ORCID= en-aut-name=OmatsuYoshiharu en-aut-sei=Omatsu en-aut-mei=Yoshiharu kn-aut-name=大松義治 kn-aut-sei=大松 kn-aut-mei=義治 aut-affil-num=2 ORCID= en-aut-name=InoueSuemi en-aut-sei=Inoue en-aut-mei=Suemi kn-aut-name=井上末瑞 kn-aut-sei=井上 kn-aut-mei=末瑞 aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=4 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=5 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Streptomyces kn-keyword=Streptomyces en-keyword=antibacterial compoud kn-keyword=antibacterial compoud en-keyword=nocardamine kn-keyword=nocardamine en-keyword=non-validamycin producing condition kn-keyword=non-validamycin producing condition END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=4 article-no= start-page=1086 end-page=1089 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Three antinematodal diterpenes from Euphorbia kansui en-subtitle= kn-subtitle= en-abstract= kn-abstract=Three compounds, 20-O-acetyl-[3-O-(2'E,4'Z)-deca-dienoyl]-ingenol (1), 20-O-acetyl-[5-O-(2'E,4'Z)-decadienoyl]-ingenol (2) and 3-O-(2'E,4'Z)-decadienoylingenol (3), were isolated from Euphorbia kansui under the bioassay-guided method. Each compound showed the same antinematodal activity against the nematode, Bursaphelenchus xylophilus, at a minimum effective dose (MED) of 5 mu g/cotton ball. en-copyright= kn-copyright= en-aut-name=ShiJian-Xiao en-aut-sei=Shi en-aut-mei=Jian-Xiao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=LiZhi-Xuan en-aut-sei=Li en-aut-mei=Zhi-Xuan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NitodaTeruhiko en-aut-sei=Nitoda en-aut-mei=Teruhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IzumiMinoru en-aut-sei=Izumi en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=College of Life Science, Northwest University affil-num=2 en-affil= kn-affil=College of Life Science, Northwest University affil-num=3 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=4 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=5 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=6 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=7 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=8 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University en-keyword=antinematodal activity kn-keyword=antinematodal activity en-keyword=Bursaphelenchus xylophilus kn-keyword=Bursaphelenchus xylophilus en-keyword=diterpenoid kn-keyword=diterpenoid en-keyword=ingenane kn-keyword=ingenane en-keyword=Euphorbia kansui kn-keyword=Euphorbia kansui END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue= article-no= start-page=1 end-page=2 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=岡山大学農学部学術報告100巻の発刊に際して en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学農学部 END