ID | 52889 |
FullText URL | |
Author |
Tsuchiya, Hiroki
Mizutani, Masahide
Kaken ID
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Abstract | Objective
To develop an objective method for assessing nociceptive behaviour in an animal model of capsaicin-induced intraoral pain. Changes in nociceptive responses were also examined after injury to the inferior alveolar nerve (IAN).
Design
Nociceptive responses evoked by the intraoral application of various doses of capsaicin were analyzed in lightly anaesthetized rats. The number of c-Fos protein-like immunoreactive (Fos-LI) neurons in the medullary dorsal horn (MDH) induced by the intraoral application of capsaicin was measured. Behavioural and c-Fos responses were also examined 14 days after injury to the IAN.
Results
Larger doses of intraoral capsaicin (1, 10 and 100 μg) induced vigorous licking behaviour and c-Fos response in the MDH in a reproducible manner. The magnitudes of both behavioural activity and the c-Fos response from the 10 and 100 μg doses of capsaicin were significantly greater than that by the 1 μg dose. Injury to the IAN exaggerated the behavioural and c-Fos responses evoked by intraoral capsaicin.
Conclusions
The intraoral application of capsaicin is a valid and reliable method for studying intraoral pain and hyperalgesia following nerve injury.
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Keywords | Capsaicin
Behaviour
c-Fos
Medullary dorsal horn
Immunohistochemistry
Nerve injury
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Published Date | 2014-12
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Publication Title |
Archives of Oral Biology
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Volume | volume59
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Issue | issue12
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Publisher | Pergamon-Elsevier Science Ltd.
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Start Page | 1334
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End Page | 1341
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ISSN | 0003-9969
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NCID | AA00548457
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Content Type |
Journal Article
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language |
English
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Copyright Holders | © 2014 Elsevier Ltd. All rights reserved.
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File Version | author
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Refereed |
True
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DOI |