ID | 59967 |
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Nemoto, Michiko
Graduate School of Environmental and Life Science, Okayama University
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Iwaki, Sayako
Graduate School of Environmental and Life Science, Okayama University
Moriya, Hisao
Graduate School of Environmental and Life Science, Okayama University
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Monden, Yuki
Graduate School of Environmental and Life Science, Okayama University
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Tamura, Takashi
Graduate School of Environmental and Life Science, Okayama University
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Inagaki, Kenji
Graduate School of Environmental and Life Science, Okayama University
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Mayama, Shigeki
Department of Biology, Tokyo Gakugei University
Obuse, Kiori
Graduate School of Environmental and Life Science, Okayama University
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Abstract | Silica cell walls of diatoms have attracted attention as a source of nanostructured functional materials and have immense potential for a variety of applications. Previous studies of silica cell wall formation have identified numerous involved proteins, but most of these proteins are species-specific and are not conserved among diatoms. However, because the basic process of diatom cell wall formation is common to all diatom species, ubiquitous proteins and molecules will reveal the mechanisms of cell wall formation. In this study, we assembled de novo transcriptomes of three diatom species, Nitzschia palea, Achnanthes kuwaitensis, and Pseudoleyanella lunata, and compared protein-coding genes of five genome-sequenced diatom species. These analyses revealed a number of diatom-specific genes that encode putative endoplasmic reticulum-targeting proteins. Significant numbers of these proteins showed homology to silicanin-1, which is a conserved diatom protein that reportedly contributes to cell wall formation. These proteins also included a previously unrecognized SET domain protein methyltransferase family that may regulate functions of cell wall formation-related proteins and long-chain polyamines. Proteomic analysis of cell wall-associated proteins in N. palea identified a protein that is also encoded by one of the diatom-specific genes. Expression analysis showed that candidate genes were upregulated in response to silicon, suggesting that these genes play roles in silica cell wall formation. These candidate genes can facilitate further investigations of silica cell wall formation in diatoms.
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Keywords | Biomineralization
Diatom
Silica
Transcriptome
Proteome
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Note | This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10126-020-09976-1.
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Published Date | 2020-06-03
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Publication Title |
Marine Biotechnology
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Publisher | Springer
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ISSN | 1436-2228
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NCID | AA11357643
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Content Type |
Journal Article
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language |
French
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OAI-PMH Set |
岡山大学
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File Version | author
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Related Url | isVersionOf https://doi.org/10.1007/s10126-020-09976-1
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Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
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助成番号 | 18K05818
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