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ID 61254
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Author
Sakai, Shunsuke Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Hatabu, Toshimitsu Laboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama University Kaken ID researchmap
Yamamoto, Yuki Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University Kaken ID researchmap
Kimura, Koji Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Abstract
After parturition, cows frequently develop uterine bacterial infections, resulting in the onset of endometritis. To eliminate the bacteria, bovine endometrial cells secrete chemokines, such as IL-6 and MCP1, which attract macrophages (M Phi s) to the subepithelial stroma. These attracted M Phi s are not only involved in bacterial elimination but also the orchestration of inflammation and tissue repair. These immune responses aid in the recovery from endometritis; however, the recovery from endometritis takes longer in summer than in any other season. Based on these findings, we hypothesized that heat stress (HS) affects the chemokine production in endometrial cells. To confirm this hypothesis, we compared IL-6 and MCP1 production induced by lipopolysaccharide (LPS) in bovine endometrial epithelial and stromal cells under normal (38.5 degrees C) and HS conditions (40.5 degrees C). In the endometrial epithelial cells, IL-6 production stimulated by LPS was significantly (p < .05) suppressed under HS conditions. MCP1 production in endometrial epithelial cells was not detected under both the control and HS conditions regardless of the presence of LPS. Moreover, LPS significantly (p < .05) stimulated IL-6 and MCP1 production in endometrial stromal cells. Moreover, HS significantly (p < .05) enhanced their production compared to that under the control conditions. In addition, HS did not affect the migration ability of M Phi s; however, the supernatant of the endometrial stromal cells cultured under the HS condition significantly (p < .05) attracted the M Phi s when compared to the control condition. These results suggest that HS disrupts chemokine production in two types of endometrial cells and alters the distribution of M Phi s in the endometrium during the summer.
Keywords
chemokine
cow
endometrial cells
endometritis
heat stress
Published Date
2020-11-23
Publication Title
Physiological Reports
Volume
volume8
Issue
issue22
Publisher
Wiley
Start Page
e14640
ISSN
2051-817X
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 The Authors.
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publisher
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DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.14814/phy2.14640
License
https://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
19H03105