JaLCDOI 10.18926/AMO/30944
FullText URL fulltext.pdf
Author Kuramoto, Hiroyuki| Hongo, Atsushi| Liu, Yi-xuan| Ojima, Yojiro| Nakamura, Keiichiro| Seki, Noriko| Kodama, Junichi| Hiramatsu, Yuji|
Abstract

The insulin-like growth factor I receptor (IGF-IR) is exceptionally overexpressed in many cervicalcancer-derived cell lines. It is postulated that a decrease of p53 protein levels due to human papillomavirus (HPV) infection may contribute to the up-regulation of IGF-IR expression in cervical cancer cells because transcription of IGF-IR is strictly down-regulated by p53. To evaluate this fact in clinical cervical cancer specimens, we checked the expression levels and activated status of IGF-IR by immunohistochemistry. Formalin-fixed and paraffin-embedded specimens obtained by conization or hysterectomy were stained with anti-IGF-IR and with an antibody recognizing phosphorylated tyrosine at its c-terminus. The expression levels of IGF-IR were significantly high in cervical intraepithelial neoplasia (CIN) III and invasive cancer specimens. Phosphorylation of IGF-IR was promoted in all CIN and invasive cancer specimens, and its intensity was related to the promotion of lesions. Interestingly, IGF-IR overexpression was missing in the basal layer of CIN I and II lesions, whereas it was evenly distributed in CIN III and invasive cancer lesions. This IGF-IR overexpression pattern may be utilized in the diagnosis of HPV infection status in CIN lesions.

Keywords insulin-like growth factor I receptor cervical cancer human papillomavirus tyrosil phosphorylation
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2008-08
Volume volume62
Issue issue4
Publisher Okayama University Medical School
Start Page 251
End Page 259
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 18766208
Web of Science KeyUT 000258680900005
JaLCDOI 10.18926/AMO/31723
FullText URL fulltext.pdf
Author Araki, Shinako| Miyagi, Yasunari| Kawanishi, Kunihiro| Yamamoto, Junko| Hongo, Atsushi| Kodama, Junichi| Yoshinouchi, Mitsuo| Kudo, Takafumi|
Abstract

The in vitro radiosensitizing effects of docetaxel have been reported, but the DNA damage caused by the irradiation after docetaxel exposure has not been investigated. In this study, the authors attempted to evaluate the radiosensitizing effects in terms of cell survival and DNA single-strand breaks in a human ovarian adenocarcinoma cell line (known as line BG-1) and a human cervical squamous cell carcinoma cell line (known as line SiHa). The cell lines were exposed to various concentrations of docetaxel (from 2.27 x 10(-3) to 2.27 microg/ml) to investigate the cytocidal effects by colony-formation assay. DNA single-strand breaks after exposure to 2.27 microg/ml of docetaxel for 30 min or 100 min were measured by the alkaline-elution assay. The remarkable cytotoxicity of docetaxel followed by irradiation was observed when concentrations were greater than 2.27 x 10(-2) microg/ml in both cell lines. The combination of docetaxel and irradiation appears to be supraadditive. The DNA single-strand breaks induced by the irradiation were enhanced in both cell lines (BG-1; P < 0.01, SiHa; P < 0.05). The synergistic cytocidal effect cannot be explained quantitatively only by the single-strand breaks.

Keywords docetaxel DNA single-strand break radiosensitizer
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 2002-02
Volume volume56
Issue issue1
Publisher Okayama University Medical School
Start Page 13
End Page 18
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 11873939
Web of Science KeyUT 000174031300003
JaLCDOI 10.18926/AMO/48081
FullText URL 66_1_53.pdf
Author Nakamura, Keiichiro| Hongo, Atsushi| Kodama, Junichi| Hiramatsu, Yuji|
Abstract The purpose of this study was to evaluate prognostic factors for epithelial ovarian cancer. We found that the pretreatment values of maximum standardized uptake (SUVmax) of the primary tumor by positron emission tomography/computed tomography (PET/CT), tumor marker CA125 and C-reactive protein (CRP) were correlated with clinical characteristics and prognosis for such patients. The clinical parameters and prognoses and their correlations with SUVmax of primary tumor, CA125 and CRP were examined for 51 patients with primary ovarian cancer. The SUVmax of the primary tumor had a statistically significant association with stage (p=0.010) and histology (p=0.001). CA125 was significant associated with stage (p=0.011), histology (p=0.005) and lymph node metastasis (p=0.025). CRP was also significantly associated with stage (p=0.049). Disease-free survival rates of patients exhibiting a high SUVmax, CA125 and CRP were significantly lower than those exhibiting a low SUVmax, CA125 and CRP levels (p=0.008, 0.034, and 0.037, respectively). Furthermore, overall survival rates of patients exhibiting a high SUVmax were significantly lower than those exhibiting a low SUVmax (p=0.049).The high SUVmax of primary tumor is an important factor for identifying ovarian cancer patients with a predictor for poor prognosis.
Keywords ovarian cancer SUVmax of primary tumor CA125 C-reactive protein predictor for poor prognosis
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2012-02
Volume volume66
Issue issue1
Publisher Okayama University Medical School
Start Page 53
End Page 60
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2012 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22358139
Web of Science KeyUT 000300800700007
JaLCDOI 10.18926/AMO/61438
FullText URL 75_1_79.pdf
Author Yoshio, Kotaro| Nagasaka, Hisako| Hisazumi, Kento| Okawa, Hiro| Tajiri, Nobuhisa| Shiode, Tsuyoki| Akaki, Shiro| Kanazawa, Susumu| Mitoma, Tomohiro| Yano, Yuri| Kobayashi, Emiko| Horiguchi, Ikuyo| Takata, Masayo| Hongo, Atsushi| Yonezawa, Masaru| Nakanishi, Yoshie|
Abstract The purposes of this retrospective study were to analyze local control of squamous cell carcinoma of the cervix treated with computed tomography (CT)-based image-guided brachytherapy (IGBT), as well as the factors affecting local control. A total of 39 patients were analyzed. The prescribed dose to the pelvis was 45-50 Gy with or without central shielding (CS). IGBT was delivered in 1-5 fractions. The total dose for high-risk clinical target volume (HR-CTV) was calculated as the biologically equivalent dose in 2-Gy fractions. The median follow-up period was 29.3 months. The 2-year overall survival and local control rates were 97% and 91%, respectively. In univariate analysis, the dose covering 90% of the HR-CTV (D90) and tumor size were found to be significant factors for local control. The cutoff values of tumor size and D90 for local control were 4.3 cm (area under the curve [AUC] 0.75) and 67.7 Gy (AUC 0.84) in the CS group and 5.3 cm (AUC 0.75) and 73.7 Gy (AUC 0.78) in the group without CS, respectively. However, though the local control of CT-based IGBT was favorable, the results suggested that the dose required for tumor control may differ depending on the presence of CS.
Keywords cervical cancer squamous cell cancer brachytherapy central shielding
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2021-02
Volume volume75
Issue issue1
Publisher Okayama University Medical School
Start Page 79
End Page 85
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2021 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 33649617
Author 本郷 淳司|
Published Date 1994-03-31
Publication Title
Content Type Thesis or Dissertation
Author 本郷 淳司|
Published Date 2005-05-20
Publication Title 岡山医学会雑誌
Volume volume117
Issue issue1
Content Type Journal Article