start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3
article-no=
start-page=119
end-page=131
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200601
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Ribosome rescue activity of an Arabidopsis thaliana ArfB homolog
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A homolog of the bacterial ribosome rescue factor ArfB was identified in Arabidopsis thaliana. The factor, named AtArfB for Arabidopsis thaliana ArfB, showed ribosome rescue activity in both in vivo and in vitro assays based on the bacterial translation system. As has been shown for ArfB, the ribosome rescue activity of AtArfB was dependent on the GGQ motif, the crucial motif for the function of class I release factors and ArfB. The C-terminal region of AtArfB was also important for its function. The N-terminal region of AtArfB, which is absent in bacterial ArfB, functioned as a transit peptide for chloroplast targeting in tobacco cells. These results strongly suggest that AtArfB is a ribosome rescue factor that functions in chloroplasts.
en-copyright=
kn-copyright=

en-aut-name=NagaoMichiaki
en-aut-sei=Nagao
en-aut-mei=Michiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TsuchiyaFumina
en-aut-sei=Tsuchiya
en-aut-mei=Fumina
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MotohashiReiko
en-aut-sei=Motohashi
en-aut-mei=Reiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=AboTatsuhiko
en-aut-sei=Abo
en-aut-mei=Tatsuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University 
kn-affil=

affil-num=2
en-affil=Graduate School of Integrated Science and Technology, Shizuoka University 
kn-affil=

affil-num=3
en-affil=Graduate School of Integrated Science and Technology, Shizuoka University
kn-affil=

affil-num=4
en-affil=Graduate School of Natural Science and Technology, Okayama University 
kn-affil=
en-keyword=Arabidopsis thaliana
kn-keyword=Arabidopsis thaliana
en-keyword=ArfB
kn-keyword=ArfB
en-keyword=chloroplast
kn-keyword=chloroplast
en-keyword=ribosome rescue
kn-keyword=ribosome rescue
en-keyword=translation
kn-keyword=translation
END