start-ver=1.4
cd-journal=joma
no-vol=24
cd-vols=
no-issue=6
article-no=
start-page=670
end-page=675
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=201411
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Increase of DC-LAMP+ mature dendritic cell subsets in dermatopathic lymphadenitis of mycosis fungoides
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Little is known about the immunological milieu of the skin-draining lymph nodes (LNs) in mycosis fungoides (MF). Objectives: We studied dendritic cell (DC) subsets in the dermatopathic lymphadenitis of MF patients. Methods: We immunohistochemically examined DC subsets and their distribution in 16 LN samples from 14 patients with MF (N1 LN, eight patients; N2, four; and N3, four), and we compared them with non-metastatic sentinel LNs from eight patients with melanoma. Results: The number of S-100 protein+ DCs was markedly increased in the LNs from the MF patients and the major component was DC-LAMP+ mature DCs in the outer and paracortex areas, where DC-SIGN+ immature DCs were relatively decreased in proportion. In contrast, DC-SIGN+ cells were relatively increased in proportion compared to DC-LAMP+ cells in the medulla. Although no significant difference was observed in the proportions of CD1a+ or Langerin+ DCs among the N1, N2, and N3 nodes, CD163+ M2-type macrophages were increased in number in the N2 and N3 nodes. Conclusions: Our observations indicate that mature DCs accumulate in the outer and paracortex areas in dermatopathic lymphadenitis and M2-type macrophages might increase in number during disease progression.
en-copyright=
kn-copyright=

en-aut-name=TadaKotaro
en-aut-sei=Tada
en-aut-mei=Kotaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HamadaToshihisa
en-aut-sei=Hamada
en-aut-mei=Toshihisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AsagoeKenji
en-aut-sei=Asagoe
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=UmemuraHiroshi
en-aut-sei=Umemura
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=Mizuno-IkedaKazuko
en-aut-sei=Mizuno-Ikeda
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=AoyamaYumi
en-aut-sei=Aoyama
en-aut-mei=Yumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OtsukaMasaki
en-aut-sei=Otsuka
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YamasakiOsamu
en-aut-sei=Yamasaki
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=IwatsukiKeiji
en-aut-sei=Iwatsuki
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=2
en-affil=
kn-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=3
en-affil=
kn-affil=Department of Dermatology, Okayama Medical Center

affil-num=4
en-affil=
kn-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=5
en-affil=
kn-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=6
en-affil=
kn-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=7
en-affil=
kn-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=8
en-affil=
kn-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=9
en-affil=
kn-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
en-keyword=Mycosis fungoides
kn-keyword=Mycosis fungoides
en-keyword=dendritic cell
kn-keyword=dendritic cell
en-keyword=dermatopathic lymphadenitis
kn-keyword=dermatopathic lymphadenitis
en-keyword=DC-LAMP
kn-keyword=DC-LAMP
en-keyword=DC-SIGN
kn-keyword=DC-SIGN
en-keyword=M2-type macrophage
kn-keyword=M2-type macrophage
END

start-ver=1.4
cd-journal=joma
no-vol=125
cd-vols=
no-issue=3
article-no=
start-page=217
end-page=220
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20131202
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Th2 cytokines increase kallikrein 7 expression and function in patients with atopic dermatitis
kn-title=Th２サイトカインはアトピー性皮膚炎患者における カリクレイン７の発現と機能を増強する
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=MorizaneShin
en-aut-sei=Morizane
en-aut-mei=Shin
kn-aut-name=森実真
kn-aut-sei=森実
kn-aut-mei=真
aut-affil-num=1
ORCID=

en-aut-name=YamasakiKenshi
en-aut-sei=Yamasaki
en-aut-mei=Kenshi
kn-aut-name=山崎研志
kn-aut-sei=山崎
kn-aut-mei=研志
aut-affil-num=2
ORCID=

en-aut-name=KajitaAi
en-aut-sei=Kajita
en-aut-mei=Ai
kn-aut-name=梶田藍
kn-aut-sei=梶田
kn-aut-mei=藍
aut-affil-num=3
ORCID=

en-aut-name=IkedaKazuko
en-aut-sei=Ikeda
en-aut-mei=Kazuko
kn-aut-name=池田佳寿子
kn-aut-sei=池田
kn-aut-mei=佳寿子
aut-affil-num=4
ORCID=

en-aut-name=ZhanMaosheng
en-aut-sei=Zhan
en-aut-mei=Maosheng
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=AoyamaYumi
en-aut-sei=Aoyama
en-aut-mei=Yumi
kn-aut-name=青山裕美
kn-aut-sei=青山
kn-aut-mei=裕美
aut-affil-num=6
ORCID=

en-aut-name=Richard L Gallo
en-aut-sei=Richard L Gallo
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IwatsukiKeiji
en-aut-sei=Iwatsuki
en-aut-mei=Keiji
kn-aut-name=岩月啓氏
kn-aut-sei=岩月
kn-aut-mei=啓氏
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　皮膚科学

affil-num=2
en-affil=
kn-affil=東北大学大学院医学系研究科　皮膚科学

affil-num=3
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　皮膚科学

affil-num=4
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　皮膚科学

affil-num=5
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　皮膚科学

affil-num=6
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　皮膚科学

affil-num=7
en-affil=
kn-affil=米国カリフォルニア大学サンディエゴ校医学部　皮膚科学

affil-num=8
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　皮膚科学
en-keyword=アトピー性皮膚炎
kn-keyword=アトピー性皮膚炎
en-keyword=Th２サイトカイン
kn-keyword=Th２サイトカイン
en-keyword=カリクレイン
kn-keyword=カリクレイン
en-keyword=表皮角化細胞
kn-keyword=表皮角化細胞
END

start-ver=1.4
cd-journal=joma
no-vol=167
cd-vols=
no-issue=2
article-no=
start-page=252
end-page=261
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201208
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Detection of antibodies against the non-calcium-dependent epitopes of desmoglein 3 in pemphigus vulgaris and their pathogenic significance
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background Antidesmoglein (anti-Dsg) 3 serum antibody titres are usually correlated with the disease activity of pemphigus vulgaris (PV), but some patients retain high titres even in remission. 

Objectives The aim of our study was to determine whether anti-Dsg3 antibodies in PV sera recognized calcium (Ca2+)-dependent or non-Ca2+-dependent epitopes, and to evaluate their pathogenicity. 

Methods Dsg3 baculoprotein-coated enzyme-linked immunosorbent assay (ELISA) plates were treated with 0.5 mmol L-1 ethylenediaminetetraacetic acid (EDTA). The binding ability of anti-Dsg3 monoclonal antibodies (mAbs) was analysed. Eight of the 83 patients with PV who were screened had elevated Dsg3 ELISA index values > 100 in remission. The binding ability of these PV sera was analysed. We evaluated the pathogenicity of anti-Dsg3 serum antibodies against the non-Ca2+-dependent epitopes using a dissociation assay. 

Results The reactivity of pathogenic anti-Dsg3 mAbs against the Ca2+-dependent epitopes diminished markedly in the EDTA-treated ELISA, whereas no such reduction was observed in mAbs against the non-Ca2+-dependent epitopes. The sera of all the patients contained antibodies against both Ca2+-dependent and non-Ca2+-dependent epitopes. In six out of the eight patients, the ratio of antibodies against Ca2+-dependent to non-Ca2+-dependent epitopes decreased in remission. EDTA-treated Dsg3 baculoproteins adsorbed anti-Dsg3 serum antibodies against the non-Ca2+-dependent epitopes, but the remnant PV antibodies retained the ability to induce acantholysis in the dissociation assay. 

Conclusions We have established an assay to measure indirectly the titres of anti-Dsg3 serum antibodies against the Ca2+-dependent epitopes, based on the differences between EDTA-untreated and EDTA-treated ELISA index values, as a routine laboratory test to reflect the pathogenic anti-Dsg3 serum antibody titres more accurately.
en-copyright=
kn-copyright=

en-aut-name=KamiyaK
en-aut-sei=Kamiya
en-aut-mei=K
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=AoyamaY
en-aut-sei=Aoyama
en-aut-mei=Y
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ShirafujiY
en-aut-sei=Shirafuji
en-aut-mei=Y
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HamadaT
en-aut-sei=Hamada
en-aut-mei=T
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MorizaneS
en-aut-sei=Morizane
en-aut-mei=S
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=FujiiK
en-aut-sei=Fujii
en-aut-mei=K
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HisataK
en-aut-sei=Hisata
en-aut-mei=K
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IwatsukiK
en-aut-sei=Iwatsuki
en-aut-mei=K
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci

affil-num=2
en-affil=
kn-affil=Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci

affil-num=3
en-affil=
kn-affil=Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci

affil-num=4
en-affil=
kn-affil=Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci

affil-num=5
en-affil=
kn-affil=Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci

affil-num=6
en-affil=
kn-affil=Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci

affil-num=7
en-affil=
kn-affil=Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci

affil-num=8
en-affil=
kn-affil=Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci
END