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ID 61553
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Author
Kato, Koji Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Miyazaki, Naoyuki Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba
Hamaguchi, Tasuku Biostructural Mechanism Laboratory, RIKEN Spring-8 Center
Nakajima, Yoshiki Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University ORCID
Akita, Fusamichi Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Yonekura, Koji Institute of Multidisciplinary Research for Advanced Materials, Tohoku University
Shen, Jian-Ren Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Abstract
Photosystem II (PSII) plays a key role in water-splitting and oxygen evolution. X-ray crystallography has revealed its atomic structure and some intermediate structures. However, these structures are in the crystalline state and its final state structure has not been solved. Here we analyzed the structure of PSII in solution at 1.95 Å resolution by single-particle cryo-electron microscopy (cryo-EM). The structure obtained is similar to the crystal structure, but a PsbY subunit was visible in the cryo-EM structure, indicating that it represents its physiological state more closely. Electron beam damage was observed at a high-dose in the regions that were easily affected by redox states, and reducing the beam dosage by reducing frames from 50 to 2 yielded a similar resolution but reduced the damage remarkably. This study will serve as a good indicator for determining damage-free cryo-EM structures of not only PSII but also all biological samples, especially redox-active metalloproteins.
Published Date
2021-03-22
Publication Title
Communications Biology
Volume
volume4
Issue
issue1
Publisher
Springer
Start Page
382
ISSN
2399-3642
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
File Version
publisher
DOI
Related Url
isVersionOf https://doi.org/10.1038/s42003-021-01919-3
License
http://creativecommons.org/licenses/by/4.0/