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ID 58571
Author
Morita, Daichi Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Takahashi, Eizo Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Morita, Masatomo Department of Bacteriology I, National Institute of Infectious Diseases
Ohnishi, Makoto Department of Bacteriology I, National Institute of Infectious Diseases
Mizuno, Tamaki Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Miyoshi, Shin‐ichi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Dutta, Devarati Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Ramamurthy, Thandavarayan Center for Human Microbial Ecology, Translational Health Science and Technology Institute
Chowdhury, Goutam Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Mukhopadhyay, Asish K. Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Okamoto, Keinosuke Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Abstract
Non‐O1/non‐O139 nontoxigenic Vibrio cholerae associated with cholera‐like diarrhea has been reported in Kolkata, India. However, the property involved in the pathogenicity of these strains has remained unclear. The character of 25 non‐O1/non‐O139 nontoxigenic V. cholerae isolated during 8 years from 2007 to 2014 in Kolkata was examined. Determination of the serogroup showed that the serogroups O6, O10, O35, O36, O39, and O70 were represented by two strains in each serogroup, and the remaining isolates belonged to different serogroups. To clarify the character of antibiotic resistance of these isolates, an antibiotic resistance test and the gene analysis were performed. According to antimicrobial drug susceptibility testing, 13 strains were classified as drug resistant. Among them, 10 strains were quinolone resistant and 6 of the 13 strains were resistant to more than three antibiotics. To define the genetic background of the antibiotic character of these strains, whole‐genome sequences of these strains were determined. From the analysis of these sequences, it becomes clear that all quinolone resistance isolates have mutations in quinolone resistance‐determining regions. Further research on the genome sequence showed that four strains possess Class 1 integrons in their genomes, and that three of the four integrons are found to be located in their genomic islands. These genomic islands are novel types. This indicates that various integrons containing drug resistance genes are spreading among V. cholerae non‐O1/non‐O139 strains through the action of newly generated genomic islands.
Keywords
antibiotic resistance
diarrhea
genome sequence
genomic island
integron
Vibrio cholerae
Note
This fulltext is available in Mar. 2021.
Published Date
2020-03-28
Publication Title
Microbiology and Immunology
Volume
volume64
Issue
issue6
Publisher
Wiley
Start Page
435
End Page
444
ISSN
03855600
NCID
AA00738350
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
File Version
author
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersonOf https://doi.org/10.1111/1348-0421.12790
Funder Name
Ministry of Education, Culture, Sports, Science and Technology