FullText URL Mod_Rheumatol_26_6_940.pdf fig.pdf tbl.jpg fig_all.zip
Author Hara, Chikako| Kubota, Satoshi| Nishida, Takashi| Hiasa, Miki| Hattori, Takako| Aoyama, Eriko| Moriyama, Yoshinori| Kamioka, Hiroshi| Takigawa, Masaharu|
Abstract OBJECTIVES: Platelet-rich plasma (PRP) has been widely used to enhance the regeneration of damaged joint tissues, such as osteoarthritic and rheumatoid arthritic cartilage. The aim of this study is to clarify the involvement of all of the CCN family proteins that are crucially associated with joint tissue regeneration. METHODS: Cyr61-CTGF-NOV (CCN) family proteins in human platelets and megakaryocytic cells were comprehensively analyzed by Western blotting analysis. Production of CCN family proteins in megakaryocytes in vivo was confirmed by immunofluorescence analysis of mouse bone marrow cells. Effects of CCN family proteins found in platelets on chondrocytes were evaluated by using human chondrocytic HCS-2/8 cells. RESULTS: Inclusion of CCN2, a mesenchymal tissue regenerator, was confirmed. Of note, CCN3, which counteracts CCN2, was newly found to be encapsulated in platelets. Interestingly, these two family members were not detectable in megakaryocytic cells, but their external origins were suggested. Furthermore, we found for the first time CCN5 and CCN1 that inhibits ADAMTS4 in both platelets and megakaryocytes. Finally, application of a CCN family cocktail mimicking platelets onto HCS-2/8 cells enhanced their chondrocytic phenotype. CONCLUSIONS: Multiple inclusion of CCN1, 2 and 3 in platelets was clarified, which supports the harmonized regenerative potential of PRP in joint therapeutics.
Keywords CCN family Cartilage Megakaryocyte Platelet Regeneration
Note This is an Accepted Manuscript of an article published by Taylor & Francis Group This fulltext availavle in Nov 2017
Published Date 2016-11
Publication Title Modern Rheumatology
Volume volume26
Issue issue6
Publisher Taylor & Francis
Start Page 940
End Page 949
ISSN 1439-7595
NCID AA1157187X
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
File Version author
PubMed ID 26915735
DOI 10.3109/14397595.2016.1155255
Web of Sience KeyUT 000393121500022
Related Url isVersionOf https://doi.org/10.3109/14397595.2016.1155255
Author Aoyama, Eriko|
Published Date 2015-02
Publication Title Okayama University Medical Research Updates
Volume volume6
Content Type Others
Author Tomita, Nao| Hattori, Takako| Itoh, Shinsuke| Aoyama, Eriko| Yao, Mayumi| Yamashiro, Takashi| Takigawa, Masaharu|
Published Date 2013-03-28
Publication Title PLoS ONE
Volume volume8
Issue issue3
Content Type Journal Article
Author Itoh, Shinsuke| Hattori, Takako| Tomita, Nao| Aoyama, Eriko| Yutani, Yasutaka| Yamashiro, Takashi| Takigawa, Masaharu|
Published Date 2013-08-12
Publication Title PLoS ONE
Volume volume8
Issue issue8
Content Type Journal Article
Author Hoshijima, Mitsuhiro| Hattori, Takako| Aoyama, Eriko| Nishida, Takashi| Yamashiro, Takashi| Takigawa, Masaharu|
Published Date 2012-10
Publication Title FEBS Journal
Volume volume279
Issue issue19
Content Type Journal Article
Author Yoshihara, Ritsuko| Aoyama, Eriko| Kadota, Yusuke| Kawai, Saeko| Goto, Tomomi| Zhong, Ming| Gohda, Eiichi|
Published Date 2008-06-30
Publication Title Cellular Immunology
Volume volume250
Issue issue1-2
Content Type Journal Article
Author Aoyama, Eriko|
Published Date 2005-03-25
Publication Title
Content Type Thesis or Dissertation