Title Alternative Identification of Marker Gene of Pars Tuberalis Morphogenesis in Chicken Embryo. The expression of Cytokine-like 1 and Gap junction protein alpha 5 in the pars tuberalis
FullText URL poalas_033_031_034.pdf
Author Aizawa, Sayaka | Higaki, Yuriko| Ogoshi, Maho| Takeuchi, Sakae| Takahashi, Sumio|
Abstract Adenohypophysis delivered from oral ectoderm consists of pars distalis (PD), pars intermedia and pars tuberalis (PT). The mechanisms of development of PD has been well studied, and the cell differentiation of PD has been well understood. However, the morphogenesis and the differentiation of PT are still unclear, and the gene expression during the PT development remains largely unknown. In this study, we explored the specifically expressing genes in PT during development and analyzed its spatiotemporal expressions pattern. Microarray analysis on laser-captured PT and PD tissues obtained from chicken embryos on embryonic day 10 (E10.0) showed high expressing genes, Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) in PT. A detail analysis of spatiotemporal expressions pattern during chick embryo development by in situ hybridization revealed that CYTL1 mRNA was first detected in lateral head ectoderm and ventral head ectoderm in E1.5. The CYTL1 expressions moved into Rathke’s pouch at E2.5, then it was localized in PT primordium and continuously expressed in PT primordium until E12.0. On the other hand, GJA5 mRNA was transiently detected in PT primordium from E6 to E14.0, while the expression was not detected in PD during development. These results suggested that these genes may be involved in the regulation mechanism of PT development and could be a useful marker in the PT development.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2017-04
Volume volume33
Start Page 31
End Page 34
language 日本語
File Version publisher
Author Kugimoto, Ayako| Aizawa, Sayaka| Ogoshi, Maho| Takahashi, Sumio| Takeuchi, Sakae|
Published Date 2016-04
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Volume volume32
Content Type Journal Article
Author Tokumori, Megumi| Ogoshi, Maho| Takeuchi, Sakae| Takahashi, Sumio|
Published Date 2015-04
Publication Title 岡山実験動物研究会報
Volume volume31
Content Type Journal Article
Author Takahashi, Toru| Nishio, Kaori| Ogoshi, Maho| Takahashi, Sumio| Takeuchi, Sakae|
Published Date 2014-04
Publication Title 岡山実験動物研究会報
Volume volume30
Content Type Journal Article
Author Fukao, Ayaka| Nakaoka, Minori| Takahashi, Sumio| Takeuchi, Sakae|
Published Date 2012-04
Publication Title 岡山実験動物研究会報
Volume volume28
Content Type Journal Article
Author Ohtsuki, Takashi| Otsuki, Mariko| Murakami, Yousuke| Maekawa, Tetsuya| Yamamoto, Takashi| Akasaka, Koji| Takeuchi, Sakae| Takahashi, Sumio|
Published Date 2005-09
Publication Title Zoological Science
Volume volume22
Issue issue9
Content Type Journal Article
Author Kusumoto, Kenji| Murakami, Yousuke| Otsuki, Mariko| Kanayama, Munetoshi| Takeuchi, Sakae| Takahashi, Sumio|
Published Date 2005-09
Publication Title Zoological Science
Volume volume22
Issue issue9
Content Type Journal Article
Author Ohtsuki, Takashi| Otsuki, Mariko| Murakami, Yousuke| Hirata, Kensaku| Takeuchi, Sakae| Takahashi, Sumio|
Published Date 2007-03
Publication Title Zoological Science
Volume volume24
Issue issue3
Content Type Journal Article
Author Maekawa, Tetsuya| Takeuchi, Sakae| Kanayama, Munetoshi| Takahashi, Sumio|
Published Date 2009-02
Publication Title Zoological Science
Volume volume26
Issue issue2
Content Type Journal Article
Author Honda, Junichi| Takeuchi, Sakae| Fukumachi, Hiroshi| Takahashi, Sumio|
Published Date 1998-08
Publication Title Zoological Science
Volume volume15
Issue issue4
Content Type Journal Article
Author Morooka, Yoshiaki| Oomizu, Souichi| Takeuchi, Sakae| Takahashi, Sumio|
Published Date 1998-08
Publication Title Zoological Science
Volume volume15
Issue issue4
Content Type Journal Article
Author Shiraga, Masahiro| Komatsu, Noriko| Teshigawara, Kiyoshi| Okada, Akinobu| Takeuchi, Sakae| Fukamachi, Hiroshi| Takahashi, Sumio|
Published Date 2000-07
Publication Title Zoological Science
Volume volume17
Issue issue5
Content Type Journal Article
Author Kakeya, Tomoshi| Takeuchi, Sakae| Takahashi, Sumio|
Published Date 2002-07
Publication Title Zoological Science
Volume volume19
Issue issue7
Content Type Journal Article
Author Sharma, Seema| Oomizu, Souichi| Kakeya, Tomoshi| Masui, Tohru| Takeuchi, Sakae| Takahashi, Sumio|
Published Date 2003-01
Publication Title Zoological Science
Volume volume20
Issue issue1
Content Type Journal Article
Author Komatsu, Noriko| Maekawa, Tetsuya| Takeuchi, Sakae| Takahashi, Sumio|
Published Date 2003-05
Publication Title Zoological Science
Volume volume20
Issue issue5
Content Type Journal Article
Author Taniuchi, Shusuke| Takahashi, Sumio| Takeuchi, Sakae|
Published Date 2011-05
Publication Title 岡山実験動物研究会報
Volume volume27
Content Type Journal Article
Author Tsuchiya, Yukiko| Sakuma, Atsuko| Takeuchi, Sakae| Takahashi, Sumio|
Published Date 2011-05
Publication Title 岡山実験動物研究会報
Volume volume27
Content Type Journal Article
Author Takeuchi, Sakae|
Published Date 2009-03
Publication Title 岡山実験動物研究会報
Volume volume25
Content Type Others
Author Takahashi, Sumio| Honda, Junichi| Manabe, Yoshie| Matsumura, Ryusei| Takeuchi, Sakae|
Published Date 2003-09
Publication Title 岡山実験動物研究会報
Volume volume20
Content Type Journal Article
Author Takahashi, Sumio| Matsumura, Ryusei| Takeuchi, Sakae|
Published Date 2004-09
Publication Title 岡山実験動物研究会報
Volume volume21
Content Type Journal Article