Title Alternative エンドウプロトプラスト細胞における植物プロモータの発現
FullText URL 080_0051_0060.pdf
Author Yamada, Tetsuji| Hashimoto, Tadaaki| Sriprasertsak, Permpong| Kato, Hisaharu| Ichinose, Yuki| Shiraishi, Tomonori| Oku, Hachiro|
Abstract High yields of viable pea protoplasts were produced from suspension cultured cells derived from calli formed from embryogenic tissues or leaves and the conditions for the optimum expression of chloramphenicol acetyltransferase (CAT) fused to the phenylalanine ammonia-lyase gene of Pisum sativum (pPAL1-15) were investigated by transient assay after electroporation. A fungal elicitor isolated from a pea pathogen, Mycosphaerella pinodes, and the reduced from of glutathione induced the expression of PAL promoter but orthovanadate, a plasma membrane ATPase inhibitor, considerably suppressed the gene expression. Rice protoplasts were also prepared from the suspension cultured cells derived from embryonic tissues, and the effects of elicitors on the expression of CAT in pPAL1-15-electroporated rice protoplasts were examined. No distinctive induction of CAT activity was observed by the treatment of rice protoplasts with a chitosan oligomer elicitor.
Publication Title 岡山大学農学部学術報告
Published Date 1992
Volume volume80
Issue issue1
Start Page 51
End Page 60
ISSN 0474-0254
language 英語
File Version publisher
Author Zhao, Lei| Le Thi Phuong| Mai Thanh Luan| Aprilia Nur Fitrianti| Matsui, Hidenori| Nakagami, Hirofumi| Noutoshi, Yoshiteru| Yamamoto, Mikihiro| Ichinose, Yuki| Shiraishi, Tomonori| Toyoda, Kazuhiro|
Keywords Apoplastic oxidative burst Arabidopsis Cell wall Class III peroxidase PRX34 Reactive oxygen species (ROS)
Note This fulltext will be available in Jun 2020|
Published Date 2019-06-07
Publication Title Journal of General Plant Pathology
Volume volume85
Issue issue6
Publisher Springer
Start Page 405
End Page 412
ISSN 1345-2630
NCID AA11449093
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version author
DOI 10.1007/s10327-019-00863-9
Web of Science KeyUT 000491069800001
Related Url isVersionOf https://doi.org/10.1007/s10327-019-00863-9
Author Taguchi, Fumiko| Ogawa, Yujiro| Takeuchi, Kasumi| Suzuki, Tomoko| Toyoda, Kazuhiro| Shiraishi, Tomonori| Ichinose, Yuki|
Published Date 2006-12
Publication Title Journal of Bacteriology
Volume volume188
Issue issue24
Content Type Journal Article
Title Alternative クラドスポリウム属菌によるブドウの褐点病について
FullText URL 048_0017_0022.pdf
Author Ouchi, Seiji| Hatamoto, Motomu| Oku, Hachiro| Shiraishi, Tomonori| Yokoyama, Tatsuo| Tateishi, Michihiro| Fujii, Shintaro|
Abstract Cladosporium cladosporioides (Fresenius) de Vries and Cladosporium herbarum (Pers. ) Link ex Fr. were isolated from grape berries that had been commonly called 'black navel', and were found to cause the disease. In view of the colour of lesions that were observed most frequently at the stage of maturing, the disease was named brown spot of grapes.
Publication Title 岡山大学農学部学術報告
Published Date 1976
Volume volume48
Issue issue1
Start Page 17
End Page 22
ISSN 0474-0254
language 英語
File Version publisher
Author Ichinose, Yuki| Ito, Masayuki| Seki, Hikaru| Toyoda, Kazuhiro| Shiraishi, Tomonori| Yamada, Tetsuji|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Toyoda, Kazuhiro| Ichinose, Yuki| Yamada, Tetsuji| Shiraishi, Tomonori|
Published Date 1999-02
Publication Title 岡山大学農学部学術報告
Volume volume88
Issue issue1
Content Type Departmental Bulletin Paper
Title Alternative 殺菌剤、ベノミル、チウラムの土壌微生物相と2~3の土壌生息性糸状菌に及ぼす影響
FullText URL 054_0001_0008.pdf
Author Oku, Hachiro| Oki, Kazuo| Shiraishi, Tomonori| Sato, Kenji| Ouchi, Seiji|
Abstract Effects of Benomyl and Thiram on the soil microflora and some soil inhabitant fungi were studied using the_ soil (sandy loam) of the experimental field, Okayama University. Under field conditions, heavy application of Benomyl did not affect significantly the soil microflora. Thiram, however, reduced the fungal population in soil to 1/6 at the next day of treatment, but recovered to the normal level after 6 days. Under laboratory conditions, both fungicides did not affect soil microflora. Population of Benomyl-to lerant fungi was about 1/10 of the total fungi and increased slightly in the field soil by treatment with Benomyl at the later stage of experiment during June to October. Neither Thiram-tolerant fungus nor bacterium was found in both Thiram-treated and non-treated soils. A fungus highly tolerant to Benomyl was isolated and identified as Aspergillus versicolor, and found to not have the metabolic activity to degrade BCM. The absorption of BCM by the mycelia of this tolerant fungus, A. versicolor, was less than half of the BCM-sensitive one, such as Cladosporium harbarum.
Publication Title 岡山大学農学部学術報告
Published Date 1979
Volume volume54
Issue issue1
Start Page 1
End Page 8
ISSN 0474-0254
language 英語
File Version publisher
Author Sasabe, Michiko| Naito, Kana| Suenaga, Hiroko| Ikeda, Takako| Toyoda, Kazuhiro| Inagaki, Yoshishige| Shiraishi, Tomonori| Ichinose, Yuki|
Published Date 2007-01-01
Publication Title DNA Sequence
Volume volume18
Issue issue2
Content Type Journal Article
Author Fujita, Keiko| Suzuki, Tomoko| Hasegawa, Sachiko| Meguro, Akane| Sugiura, Hiroyuki| Toyoda, Kazuhiro| Shiraishi, Tomonori| Sakaguchi, Ei| Nishimura, Tomio| Kunoh, Hitoshi|
Published Date 2010-02-01
Publication Title 岡山大学農学部学術報告
Volume volume99
Issue issue1
Content Type Departmental Bulletin Paper
Author Toyoda, Kazuhiro| Matsuoka, Sachiko| Meguro, Akane| Hasegawa, Sachiko| Nishimura, Tomio| Kunoh, Hitoshi| Shiraishi, Tomonori|
Published Date 2010-02-01
Publication Title 岡山大学農学部学術報告
Volume volume99
Issue issue1
Content Type Departmental Bulletin Paper
Author Marutani, Mizuri| Taguchi, Fumiko| Ogawa, Yujiro| Hossain, Mijan Md.| Yoshishige, Inagaki| Toyoda, Kazuhiro| Shiraishi, Tomonori| Ichinose, Yuki|
Published Date 2008-04
Publication Title Molecular Genetics and Genomics
Volume volume279
Issue issue4
Content Type Journal Article
Author Taguchi, Fumiko| Takeuchi, Kasumi| Katoh, Etsuko| Murata, Katsuyoshi| Suzuki, Tomoko| Marutani, Mizuri| Kawasaki, Takayuki| Eguchi, Minako| Katoh, Shizue| kaku, Hanae| Yasuda, Chihiro| Inagaki, Yoshishige| Toyoda, Kazuhiro| Shiraishi, Tomonori| Ichinose, Yuki|
Published Date 2006-6
Publication Title Cellular Microbiology
Volume volume8
Issue issue6
Content Type Journal Article
Title Alternative エンドウ褐紋病菌の胞子発芽液に含まれるピサチン蓄積誘導物質の分離と性質
FullText URL 059_0001_0009.pdf
Author Thanutong, Porntip| Oku, Hachiro| Shiraishi, Tomonori| Ouchi, Seiji|
Abstract An elicitor of pisatin accumulation was isolated and partially characterized from spore germination fluid, mycelial extract, and cell wall preparation of a pea pathogen, Mycosphaerella pinodes. The elicitor was found to be a polysaccharide or glycoprotein and the active component was composed of glucose. The approximate molecular weight was 70,000 daltons. This elicitor elicited pisatin accumulation in pea leaves at a concentration of 10 ug/ml and the maximum activity was ca. 350-400 ug/ml, but did not elicit phytoalexins in soybean, bean, and red clover. High molecular weight preparations from the mycelial extract and mycelial wall of Mycosphaerella melonis and Stemphylium sarcinaeforme, nonpathogens of pea, elicited pisatin accumulation in pea leaves.
Publication Title 岡山大学農学部学術報告
Published Date 1982
Volume volume59
Issue issue1
Start Page 1
End Page 9
ISSN 0474-0254
language 英語
File Version publisher
Author Higashi, Kuniaki| Ishiga, Yasuhiro| Inagaki, Yoshishige| Toyoda, Kazuhiro| Shiraishi, Tomonori| Ichinose, Yuki|
Published Date 2008-03-01
Publication Title Molecular Genetics and Genomics
Volume volume279
Issue issue3
Content Type Journal Article
Author Ichinose, Yuki| Endoh, Ai| Sanematsu, Shiroh| Toyoda, Kazuhiro| Shiraishi, Tomonori| Yamada, Tetsuji|
Published Date 1999-02
Publication Title 岡山大学農学部学術報告
Volume volume88
Issue issue1
Content Type Departmental Bulletin Paper
Author Kawahara, Tomoharu| Toyoda, Kazuhiro| Kiba, Akinori| Ichinose, Yuki| Yamada, Tetsuji| Shiraishi, Tomonori|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Toyoda, Kazuhiro| Koyama, Masashi| Mizukoshi, Rumi| Ichinose, Yuki| Yamada, Tetsuji| Shiraishi, Tomonori|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Title Alternative 農業用殺菌剤、植物病原菌の有害代謝産物によるファイトアレキシンの生産
FullText URL 042_0017_0020.pdf
Author Oku, Hachiro| Nakanishi, Toshiro| Shiraishi, Tomonori| Ouchi, Seiji|
Abstract The endocarp of the fresh pea pod incubated with solution or suspension of agricultural fungicides, phytotoxic metabolites of plant pathogenic fungi formed the pea phytoalexin, pisatin. Among the compounds tested, cycloheximide, triazine, dichlone, phenyimercury acetate, UV degradation product of phenylmercury acetate, triphenyltin fungicides, and 3-hydroxy-5-methylisoxazole induced pisatin. Ophiobolin, a toxin from Cochliobolus mtyabeanus, and ascochitine, a toxic metabolite from Ascochyta fabae, also induced pisatin. The possibilities of the development of harmless plant disease control agents was discussed in relation- to the induced synthesis of phytoalexins.
Publication Title 岡山大学農学部学術報告
Published Date 1973
Volume volume42
Issue issue1
Start Page 17
End Page 20
ISSN 0474-0254
language 英語
File Version publisher
Author Seki, Hikaru| Marutani, Mizuri| Inagaki, Yoshishige| Toyoda, Kazuhiro| Shiraishi, Tomonori| Ichinose, Yuki|
Published Date 2003-02
Publication Title 岡山大学農学部学術報告
Volume volume92
Issue issue1
Content Type Departmental Bulletin Paper
Author Kudo, Chikako| Suzuki, Tomoko| Fukuoka, Sumie| Asai, Shuta| Suenaga, Hiroko| Sasabe, Michiko| Takano, Yoshitaka| Okuno, Tetsuro| Toyoda, Kazuhiro| Shiraishi, Tomonori| Ichinose, Yuki| Inagaki, Yoshi-Shige|
Published Date 2007-07-01
Publication Title Molecular Plant Pathology
Volume volume8
Issue issue4
Content Type Journal Article