ID | 11282 |
Sort Key | 5
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Title Alternative | Evaluation of Three Different Procedures for Extracting Adenylates from Barley Roots Prior to Luminometric Quantification
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FullText URL | |
Author |
Akiyama, Yoshiko
Kawasaki, Toshio
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Abstract | Three methods were compared for extracting adenylates from barley roots prior to their quantification by a lumino-metric method. In respect of efficiency in extracting adenylates and easiness in handling, the best result was obtained in the root sample which was homogenized in perchloric acid, neutralized by mixing with octylamine dissolved in 1,1,2-trichloro-1,2,2-trifluoroethane and centrifuged.
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抄録(別言語) | 高等植物根のアデニル酸をルシフェリン-ルシフェラーゼ法による定量に適する抽出法を捜すために、数種の抽出法を比べた。過塩素酸で抽出し、オクチルアミンで中和し、フロンの一種で洗う方法が最も抽出効果が高く、また比較的操作が簡便であった。
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Keywords | Adenylate extraction
ADP
AMP
ATP
Barley roots
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Publication Title |
岡山大学資源生物科学研究所報告
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Published Date | 1995
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Volume | volume3
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Issue | issue2
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Publisher | 岡山大学資源生物科学研究所
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Publisher Alternative | Research Institute for Bioresources, Okayama University
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Start Page | 137
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End Page | 143
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ISSN | 0916-930X
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NCID | AN10381600
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Content Type |
Departmental Bulletin Paper
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OAI-PMH Set |
岡山大学
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language |
Japanese
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File Version | publisher
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Refereed |
False
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