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ID 57955
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Author
Akiyama, Tomoyuki Department of Child Neurology, Okayama University Hospital Kaken ID researchmap
Saigusa, Daisuke Tohoku Medical Megabank Organization, Tohoku University
Hyodo, Yuki Department of Child Neurology, Okayama University Hospital
Umeda, Keiko Tohoku Medical Megabank Organization, Tohoku University
Saijo, Reina Tohoku Medical Megabank Organization, Tohoku University
Koshiba, Seizo Tohoku Medical Megabank Organization, Tohoku University
Kobayashi, Katsuhiro Department of Child Neurology, Okayama University Hospital Kaken ID researchmap
Abstract
To characterize metabolic profiles within the central nervous system in epilepsy, we performed gas chromatography-tandem mass spectrometry (GC-MS/MS)-based metabolome analysis of the cerebrospinal fluid (CSF) in pediatric patients with and without epilepsy. The CSF samples obtained from 64 patients were analyzed by GC-MS/MS. Multivariate analyses were performed for two age groups, 0-5 years of age and 6-17 years of age, to elucidate the effects of epilepsy and antiepileptic drugs on the metabolites. In patients aged 0-5 years (22 patients with epilepsy, 13 without epilepsy), epilepsy patients had reduced 2-ketoglutaric acid and elevated pyridoxamine and tyrosine. In patients aged 6-17 years (12 with epilepsy, 17 without epilepsy), epilepsy patients had reduced 1,5-anhydroglucitol. Valproic acid was associated with elevated 2-aminobutyric acid, 2-ketoisocaproic acid, 4-hydroxyproline, acetylglycine, methionine, N-acetylserine, and serine. Reduced energy metabolism and alteration of vitamin B6 metabolism may play a role in epilepsy in young children. The roles of 1,5-anhydroglucitol in epilepsy in older children and in levetiracetam and zonisamide treatment remain to be explained. Valproic acid influenced the levels of amino acids and related metabolites involved in the metabolism of serine, methionine, and leucine.
Keywords
antiepileptic drugs
gas chromatography-tandem mass spectrometry
metabolome analysis
metabolomics
Amo Type
Original Article
Published Date
2020-02
Publication Title
Acta Medica Okayama
Volume
volume74
Issue
issue1
Publisher
Okayama University Medical School
Start Page
65
End Page
72
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
Copyright Holders
CopyrightⒸ 2020 by Okayama University Medical School
File Version
publisher
Refereed
True
PubMed ID