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ID 31612
JaLCDOI
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Author
Funamori, Yuka
Fujinaga, Yukako
Inoue, Kaoru
Hirai, Yoshikazu
Kira, Shohei
Taketa, Kazuhisa
Abstract

Three outbreaks and many isolated cases of enterohemorrhagic Escherichia coli O157:H7 occurred in 1996 and 1997 in Okayama Prefecture, Japan. In an attempt to investigate the route of these infections, the strains isolated from the 3 outbreaks (total 33 strains) and 15 isolated cases (total 15 strains) were investigated using random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE). In addition, 10 strains from an outbreak in Tojo Cho, Hiroshima Prefecture (June 1996), 2 strains from the particular types of meat in Kochi Prefecture, and 42 strains isolated from bovine feces in a farm in Okayama Prefecture were also investigated in the same manner. PFGE was much more useful than RAPD for molecular typing of the clinical isolates, in that it allowed us to classify them into 10 PFGE groups. We noted that the strains differed according to the time and place of the outbreaks (or isolated cases). This indicates that O157:H7 infections in Okayama Prefecture were caused by different strains (although some cases were aggravated by the same strains as were found in other areas). The isolates from bovine feces were classified into 5 groups by PFGE profiles, but none of them were identical to those of the clinical isolates.

Keywords
molecular epidemiology
enterohemorrhagic Escherichia coli O157: H7
pulsed field gel electrophoresis
random amplification of polymorphic DNA
Amo Type
Article
Published Date
1999-08
Publication Title
Acta Medica Okayama
Volume
volume53
Issue
issue4
Publisher
Okayama University Medical School
Start Page
193
End Page
200
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT