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Kawakami, Natsuko Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Otubo, Akito Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Maejima, Sho Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Talukder, Ashraf H. Laboratory of Information Biology, Graduate School of Information Sciences, Tohoku University
Satoh, Keita Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Oti, Takumi Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Takanami, Keiko Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Ueda, Yasumasa Department of Physiology, Kyoto Prefectural University of Medicine
Itoi, Keiichi Laboratory of Information Biology, Graduate School of Information Sciences, Tohoku University
Morris, John F. Department of Physiology, Anatomy and Genetics, University of Oxford
Sakamoto, Tatsuya Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Sakamoto, Hirotaka Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Abstract
Arginine vasopressin (AVP) is synthesized in parvocellular‐ and magnocellular neuroendocrine neurons in the paraventricular nucleus (PVN) of the hypothalamus. Whereas magnocellular AVP neurons project primarily to the posterior pituitary, parvocellular AVP neurons project to the median eminence (ME) and to extrahypothalamic areas. The AVP gene encodes pre‐pro‐AVP that comprises the signal peptide, AVP, neurophysin (NPII), and a copeptin glycopeptide. In the present study, we used an N‐terminal copeptin antiserum to examine copeptin expression in magnocellular and parvocellular neurons in the hypothalamus in the mouse, rat, and macaque monkey. Although magnocellular NPII‐expressing neurons exhibited strong N‐terminal copeptin immunoreactivity in all three species, a great majority (~90%) of parvocellular neurons that expressed NPII was devoid of copeptin immunoreactivity in the mouse, and in approximately half (~53%) of them in the rat, whereas in monkey hypothalamus, virtually all NPII‐immunoreactive parvocellular neurons contained strong copeptin immunoreactivity. Immunoelectron microscopy in the mouse clearly showed copeptin‐immunoreactivity co‐localized with NPII‐immunoreactivity in neurosecretory vesicles in the internal layer of the ME and posterior pituitary, but not in the external layer of the ME. Intracerebroventricular administration of a prohormone convertase inhibitor, hexa‐d‐arginine amide resulted in a marked reduction of copeptin‐immunoreactivity in the NPII‐immunoreactive magnocellular PVN neurons in the mouse, suggesting that low protease activity and incomplete processing of pro‐AVP could explain the disproportionally low levels of N‐terminal copeptin expression in rodent AVP (NPII)‐expressing parvocellular neurons. Physiologic and phylogenetic aspects of copeptin expression among neuroendocrine neurons require further exploration.
Keywords
copeptin
hypothalamo‐pituitary–adrenal system
immunohistochemistry
paraventricular nucleus of the hypothalamus
processing
vasopressin
RRID: AB_2722604
RRID: AB_2061966
RRID: AB_2314234
RRID: AB_10013361
RRID: AB_2313960
RRID: AB_2722605
RRID: AB_90782
Published Date
2020-09-06
Publication Title
Journal of Comparative Neurology
Publisher
Wiley
ISSN
0021-9967
NCID
AA00695917
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 The Authors.
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publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1002/cne.25026
License
https://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Agency for Medical Research and Development
助成番号
961149