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ID 55199
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Author
Tamura, Kei Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Kobayashi, Yasuhisa Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Hirooka, Asuka Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Takanami, Keiko Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Oti, Takumi Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Jogahara, Takamichi Laboratory of Animal Management and Resources, Department of Zoology, Okayama University of Science
Oda, Sen-ichi Laboratory of Animal Management and Resources, Department of Zoology, Okayama University of Science
Sakamoto, Tatsuya Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Sakamoto, Hirotaka Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Abstract
Several regions of the brain and spinal cord control male reproductive function. We previously demonstrated that the gastrin-releasing peptide (GRP) system, located in the lumbosacral spinal cord of rats, controls spinal centers to promote penile reflexes during male copulatory behavior. However, little information exists on the male-specific spinal GRP system in animals other than rats. The objective of this study was to examine the functional generality of the spinal GRP system in mammals using the Asian house musk shrew (Suncus murinus; suncus named as the laboratory strain), a specialized placental mammal model. Mice are also used for a representative model of small laboratory animals. We first isolated complementary DNA encoding GRP in suncus. Phylogenetic analysis revealed that suncus preproGRP was clustered to an independent branch. Reverse transcription-PCR showed that GRP and its receptor mRNAs were both expressed in the lumbar spinal cord of suncus and mice. Immunohistochemistry for GRP demonstrated that the sexually dimorphic GRP system and male-specific expression/distribution patterns of GRP in the lumbosacral spinal cord in suncus are similar to those of mice. In suncus, we further found that most GRP-expressing neurons in males also express androgen receptors, suggesting that this male-dominant system in suncus is also androgen-dependent. Taken together, these results indicate that the sexually dimorphic spinal GRP system exists not only in mice but also in suncus, suggesting that this system is a conserved property in mammals.
Keywords
RRID
AB_2060157
RRID: AB_2571636
RRID: AB_626757
Suncus murinus (suncus)
gastrin-releasing peptide
male reproductive function
sexual dimorphism
spinal cord
Note
This is the peer reviewed version of the following article: Tamura K. Kobayashi Y. Hirooka A. et al. Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus). J Comp Neurol. 2017;525:1601–1613. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
2018年5月公開予定
Published Date
2017-05-01
Publication Title
Journal of Comparative Neurology
Volume
volume525
Issue
issue7
Publisher
Wistar Institute of Anatomy and Biology
Start Page
1586
End Page
1598
ISSN
0021-9967
NCID
AA00695917
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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author
PubMed ID
Web of Science KeyUT
Related Url
https://doi.org/10.1002/cne.24138