JaLCDOI 10.18926/AMO/59957
FullText URL 74_3_237.pdf
Author Oyama, Atsushi| Uchida, Daisuke| Shiraha, Hidenori| Sawahara, Hiroaki| Kato, Ryo| Iwamuro, Masaya| Horiguchi, Shigeru| Okada, Hiroyuki|
Abstract The physiological role of the reduced expression of immortalized cells (REIC)/Dickkopf-3 (Dkk-3) protein in patients with hepatocellular carcinoma (HCC) remains unclear. In this study, we evaluated the effect of the REIC/Dkk-3 protein on HCC cell proliferation and assessed the relationship between the serum REIC/Dkk-3 protein level and the prognosis in patients with HCC. We evaluated the REIC/Dkk-3 protein-induced anticancer effects on Huh7 and Hep3B cells (HCC cell lines) in the presence of peripheral blood mononuclear cells (PBMCs), and found that combination treatment with REIC/Dkk-3 protein and PBMCs reduced the proliferation of HCC cells (Hep3B: 82.0%±16.3%; Huh7: 72.6%±9.1%). We also studied 194 HCC patients who underwent primary liver resection or primary radiofrequency ablation from 2008 to 2017. Serum REIC/Dkk-3 protein levels were measured by an enzyme-linked immunosorbent assay and compared to the prognostic data. The 3-year disease-free survival of the REIC/Dkk-3 high group was significantly higher than that in the REIC/Dkk-3 low group. In conclusion, this is the first study investigating the relationship between HCC patient survival and serum REIC/Dkk-3 protein levels in a large population. Based on the results, the serum REIC/Dkk-3 protein level should be considered a new prognostic marker for patients with HCC.
Keywords enzyme-linked immunosorbent assay liver resection primary radiofrequency ablation Huh7 Hep3B
Amo Type Original Article
Published Date 2020-06
Publication Title Acta Medica Okayama
Volume volume74
Issue issue3
Publisher Okayama University Medical School
Start Page 237
End Page 243
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2020 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 32577022
Author Tomoda, Takeshi | Kato, Hironari| Miyamoto, Kazuya| Matsumi, Akihiro| Ueta, Eijiro| Fujii, Yuuki| Saragai, Yousuke| Yamazaki, Tatsuhiro| Uchida, Daisuke| Matsumoto, Kazuyuki| Horiguchi, Shigeru| Tsutsumi, Koichiro| Okada, Hiroyuki|
Note Correction to: Surgical Endoscopy https://doi.org/10.1007/s00464-020-07385-y </br>This fulltext is available in Feb. 2021.|
Published Date 2020-02-06
Publication Title Surgical Endoscopy
Publisher Springer
ISSN 09302794
NCID AA10750363
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version author
PubMed ID 32030547
DOI 10.1007/s00464-020-07399-6
Web of Science KeyUT 000515963500002
Related Url isVersionOf https://doi.org/10.1007/s00464-020-07399-6
FullText URL fulltext.pdf
Author Uchida, Daisuke| Kato, Hironari| Matsumoto, Kazuyuki| Ishihara, Yuki| Matsumi, Akihiro| Saragai, Yosuke| Takada, Saimon| Yabe, Shuntaro| Muro, Shinichiro| Tomoda, Takeshi| Horiguchi, Shigeru| Okada, Hiroyuki|
Keywords Endoscopic ultrasound Diagnostic screening program Pancreatic diseases Biliary tract diseases
Published Date 2019-12-18
Publication Title BMC Gastroenterology
Volume volume19
Issue issue1
Publisher BMC
Start Page 220
ISSN 1471-230X
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s). 2019
File Version publisher
PubMed ID 31852458
DOI 10.1186/s12876-019-1141-7
Web of Science KeyUT 000510482800002
Related Url isVersionOf https://doi.org/10.1186/s12876-019-1141-7