JaLCDOI | 10.18926/AMO/52009 |
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FullText URL | 67_6_359.pdf |
Author | Katashima, Kazunori| Kuroda, Masahiro| Ashida, Masakazu| Sasaki, Takanori| Taguchi, Takehito| Matsuzaki, Hidenobu| Murakami, Jun| Yanagi, Yoshinobu| Hisatomi, Miki| Hara, Marina| Kato, Hirokazu| Ohmura, Yuichi| Kobayashi, Tomoki| Kanazawa, Susumu| Harada, Sosuke| Takemoto, Mitsuhiro| Ohno, Seiichiro| Mimura, Seiichi| Asaumi, Junichi| |
Abstract | It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis. |
Keywords | ADC apoptosis necrosis hyperthermia cell density |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2013-12 |
Volume | volume67 |
Issue | issue6 |
Publisher | Okayama University Medical School |
Start Page | 359 |
End Page | 367 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 24356720 |
Web of Science KeyUT | 000328915700004 |
Author | Yanagi, Yoshinobu| Fujita, Mariko| Hisatomi, Miki| Matsuzaki, Hidenobu| Konouchi, Hironobu| Katase, Naoki| Nagatsuka, Hitoshi| Asaumi, Jun-ichi| |
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Published Date | 201012 |
Publication Title | Oral Radiology |
Volume | volume26 |
Issue | issue2 |
Content Type | Journal Article |
Author | Murakami, Jun| Asaumi, Jun-ichi| Maki, Yuu| Tsujigiwa, Hidetsugu| Kuroda, Masahiro| Nagai, Noriyuki| Yanagi, Yoshinobu| Inoue, Tetsuyoshi| Kawasaki, Shoji| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji| |
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Published Date | 2004-2 |
Publication Title | Oral Oncology |
Volume | volume40 |
Issue | issue6 |
Content Type | Journal Article |
Author | Murakami, Jun| Asaumi, Jun-ichi| Kawai, Noriko| Tsujigiwa, Hidetsugu| Yanagi, Yoshinobu| Nagatsuka, Hitoshi| Inoue, Tetsuyoshi| Kokeguchi, Susumu| Kawasaki, Shoji| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji| |
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Published Date | 2005-07 |
Publication Title | Cancer Chemotherapy and Pharmacology |
Volume | volume56 |
Issue | issue1 |
Content Type | Journal Article |
Author | Maki, Yu| Murakami, Jun| Asaumi, Jun-ichi| Tsujigiwa, Hidetsugu| Nagatsuka, Hitoshi| Kokeguchi, Susumu| Fukui, Kazuhiro| Kawai, Noriko| Yanagi, Yoshinobu| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji| |
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Published Date | 2005-7 |
Publication Title | Oral Oncology |
Volume | volume41 |
Issue | issue10 |
Content Type | Journal Article |
Author | Asaumi, Jun-ichi| Yanagi, Yoshinobu| Konouchi, Hironobu| Hisatomi, Miki| Matsuzaki, Hidenobu| Kishi, Kanji| |
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Published Date | 2004-7 |
Publication Title | Oral Oncology |
Volume | volume40 |
Issue | issue6 |
Content Type | Journal Article |
Author | Hisatomi, Miki| Yanagi, Yoshinobu| Konouchi, Hironobu| Matsuzaki, Hidenobu| Takenobu, Toshihiko| Unetsubo, Teruhisa| Asaumi, Jun-ichi| |
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Published Date | 2011-02 |
Publication Title | Oral Oncology |
Volume | volume47 |
Issue | issue2 |
Content Type | Journal Article |
Author | Asaumi, Jun-ichi| Yanagi, Yoshinobu| Konouchi, Hironobu| Hisatomi, Miki| Matsuzaki, Hidenobu| Shigehara, Hiroshi| Kishi, Kanji| |
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Published Date | 2004-9 |
Publication Title | European Journal of Radiology |
Volume | volume51 |
Issue | issue3 |
Content Type | Journal Article |
FullText URL | isd-49-27.pdf |
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Author | Bamgbose, Babatunde O.| Okada, Shunsuke| Hisatomi, Miki| Yanagi, Yoshinobu| Takeshita, Yohei| Abdu, Zahrau Saleh| Ekuase, Edugie J.| Asaumi, Jun-ichi| |
Keywords | Tooth Supernumerary Odontoma Odontogenic Cyst |
Published Date | 2019-05-25 |
Publication Title | Imagining Science in Dentistry |
Volume | volume49 |
Issue | issue1 |
Publisher | Korean Academy of Oral and Maxillofacial Radiology |
Start Page | 27 |
End Page | 34 |
ISSN | 22337822 |
NCID | AA12764256 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2019 by Korean Academy of Oral and Maxillofacial Radiology |
File Version | publisher |
PubMed ID | 30941285 |
DOI | 10.5624/isd.2019.49.1.27 |
Web of Science KeyUT | 000462925200004 |
Related Url | isVersionOf https://doi.org/10.5624/isd.2019.49.1.27 |
Author | Kawakami, Shigehisa| Kodama, Naoki| Maeda, Naoto| Sakamoto, Shunichi| Oki, Kazuhiro| Yanagi, Yoshinobu| Asaumi, Jun-Ichi| Maeda, Teruta| Minagi, Shogo| |
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Published Date | 2012-01-15 |
Publication Title | Journal of Neuroscience Methods |
Volume | volume203 |
Issue | issue1 |
Content Type | Journal Article |
Author | Yamanaka, Reiko| Soga, Yoshihiko| Nawachi, Kumiko| Yanagi, Yoshinobu| Kodama, Naoki| Nakata, Takashi| Miura, Rumi| Hagawa, Misao| Takeuchi, Tetsuo| Yamane, Mieko| Morita, Manabu| Takashiba, Shogo| Asami, Jun-ichi| Minagi, Shogo| Yoshiyama, Masahiro| Shimono, Tsutomu| Kuboki, Takuo| Sasaki, Akira| Morita, Kiyoshi| |
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Published Date | 2009-06 |
Publication Title | 岡山歯学会雑誌 |
Volume | volume28 |
Issue | issue1 |
Content Type | Journal Article |
JaLCDOI | 10.18926/AMO/65492 |
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FullText URL | 77_3_273.pdf |
Author | Shimizu, Yudai| Kuroda, Masahiro| Nakamitsu, Yuki| Al-Hammad, Wlla E.| Yoshida, Suzuka| Fukumura, Yuka| Nakamura, Yoshihide| Kuroda, Kazuhiro| Kamizaki, Ryo| Imajoh, Satoshi| Tanabe, Yoshinori| Sugimoto, Kohei| Oita, Masataka| Sugianto, Irfan| Bamgbose, Babatunde O.| Yanagi, Yoshinobu| Asaumi, Junichi| |
Abstract | Diffusion kurtosis (DK) imaging (DKI), a type of restricted diffusion-weighted imaging, has been reported to be useful for tumor diagnoses in clinical studies. We developed a software program to simultaneously create DK images with apparent diffusion coefficient (ADC) maps and conducted an initial clinical study. Multi-shot echo-planar diffusion-weighted images were obtained at b-values of 0, 400, and 800 sec/mm2 for simple DKI, and DK images were created simultaneously with the ADC map. The usefulness of the DK image and ADC map was evaluated using a pixel analysis of all pixels and a median analysis of the pixels of each case. Tumor and normal tissues differed significantly in both pixel and median analyses. In the pixel analysis, the area under the curve was 0.64 for the mean kurtosis (MK) value and 0.77 for the ADC value. In the median analysis, the MK value was 0.74, and the ADC value was 0.75. The MK and ADC values correlated moderately in the pixel analysis and strongly in the median analysis. Our simple DKI system created DK images simultaneously with ADC maps, and the obtained MK and ADC values were useful for differentiating head and neck tumors from normal tissue. |
Keywords | simple diffusion kurtosis imaging mean kurtosis clinical trial head and neck tumor magnetic resonance imaging |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2023-06 |
Volume | volume77 |
Issue | issue3 |
Publisher | Okayama University Medical School |
Start Page | 273 |
End Page | 280 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | Copyright Ⓒ 2023 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 37357628 |
Web of Science KeyUT | 001026279600005 |
FullText URL | fulltext20230828-03.pdf |
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Author | Al‑Hammad, Wlla| Fujikura, Mamiko| Hisatomi, Miki| Okada, Shunsuke| Munhoz, Luciana| Kawazu, Toshiyuki| Takeshita, Yohei| Fujita, Mariko| Yanagi, Yoshinobu| Asaumi, Jun-Ichi| |
Keywords | vascular anomalies magnetic resonance imaging computed tomography dynamic contrast-enhanced magnetic resonance imaging contrast index curve |
Published Date | 2023-07-27 |
Publication Title | Oncology Letters |
Volume | volume26 |
Issue | issue3 |
Publisher | Spandidos Publications |
Start Page | 394 |
ISSN | 1792-1074 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2023 Al-Hammad et al. |
File Version | publisher |
PubMed ID | 37600333 |
DOI | 10.3892/ol.2023.13980 |
Web of Science KeyUT | 001049518700001 |
Related Url | isVersionOf https://doi.org/10.3892/ol.2023.13980 |