start-ver=1.4
cd-journal=joma
no-vol=961
cd-vols=
no-issue=2
article-no=
start-page=179
end-page=189
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2003
dt-pub=20030131
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A Periaxonal Net in the Zebrafish Central Nervous System
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We produced a monoclonal antibody, named A20, which specifically recognizes a 35 kDa protein and stains myelinated axons in
zebrafish brain. The A20 antigen is located at the outside of the myelin layer of large axons, and comprises a fine meshwork composed of
thin unit fibers about 1–2 μm in length and about 100–200 nm in thickness. The unit fibers form pentagonal and hexagonal structures,
which further polymerize into an envelope structure on the axons. The A20 monoclonal antibody did not stain neuronal cell bodies nor
synapses. Instead, the distribution of the A20 antigen was along axons, practically coincident with the distribution of myelin basic protein.
The monoclonal antibody stained only axons in the central nervous system (CNS), and not the extracellular matrix surrounding Schwann
cells. These results suggest that this antigenic meshwork (which we call the periaxonal net) is synthesized by oligodendrocytes. During the
development of the zebrafish brain, the periaxonal net appeared after the formation of myelin on the axons. The periaxonal net developed
first at the brain stem, then gradually appeared at the caudal end of the spinal cord. The thickness of the periaxonal net around the
Mauthner axon changed during development. Although the thickness of the Mauthner axon continues to grow throughout life, the
thickness of periaxonal net stopped growing at 6 months after fertilization.
en-copyright=
kn-copyright=

en-aut-name=NakayasuHiroshi
en-aut-sei=Nakayasu
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ArataNoriko
en-aut-sei=Arata
en-aut-mei=Noriko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Biology, Faculty of Science, Okayama University

affil-num=2
en-affil=
kn-affil=Department of Biology, Faculty of Science, Okayama University
en-keyword=Oligodendrocyte
kn-keyword=Oligodendrocyte
en-keyword=Monoclonal antibody
kn-keyword=Monoclonal antibody
en-keyword=Extracellular matrix
kn-keyword=Extracellular matrix
en-keyword=Central nervous system
kn-keyword=Central nervous system
END

start-ver=1.4
cd-journal=joma
no-vol=1129
cd-vols=
no-issue=1
article-no=
start-page=53
end-page=62
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=200701
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Distribution of a brain-specific extracellular matrix protein in developing and adult zebrafish
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>A monoclonal antibody (IgG) that recognizes a 53-kDa zebrafishnext brain protein was isolated and used to characterize the distribution of this protein in zebrafish.next (1) The antigen was found only in the brain and not in any other tissues such as muscle, dermis and cartilage. Within the brain, the antibody recognized extracellular matrix (ECM) outside neuronal cells. (2) Digestion by hyaluronidase released the antigen from brain tissue, and the monoclonal antibody staining was also decreased by the digestion by hyaluronidase. (3) The pattern of antigen distribution is not perineuronal, as the density of the antigen at the periphery of the cells was practically identical to that of the empty intercellular spaces. Therefore, this monoclonal antibody does not recognize the perineuronal glycocortex. (4) The antigen is distributed only in limited areas of the brain, namely in the periphery of the forebrain, the hypothalamus, the optic tectum, the interpeduncular nucleus, the cerebellum and the ventricular rim of the medulla. In the optic tectum, the antibody strongly stained the most superficial layer, and in the cerebellum, it stained the molecular but not the granular layer. These patterns of distribution are very different from those of other typical brain ECM proteins and suggest that this protein may play quite distinct roles in brain development and maintenance.</p>

en-copyright=
kn-copyright=

en-aut-name=KanaiTakahiro
en-aut-sei=Kanai
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KajimotoTaketoshi
en-aut-sei=Kajimoto
en-aut-mei=Taketoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakayasuHiroshi
en-aut-sei=Nakayasu
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University
en-keyword=Zebrafish
kn-keyword=Zebrafish
en-keyword=Extracellular matrix
kn-keyword=Extracellular matrix
en-keyword=Monoclonal antibody
kn-keyword=Monoclonal antibody
en-keyword=Brain-specific ECM
kn-keyword=Brain-specific ECM
END