start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=17
article-no=
start-page=2824
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240823
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cyclic Oligosaccharide-Induced Modulation of Immunoglobulin A Reactivity to Gut Bacteria Contributes to Alterations in the Bacterial Community Structure
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Immunoglobulin A (IgA) is a major gut antibody that coats commensal gut bacteria and contributes to shaping a stable gut bacterial composition. Although previous studies have shown that cyclic oligosaccharides, including cyclic nigerosyl-1,6-nigerose (CNN) and cyclodextrins (CDs, including alpha CD, beta CD, and gamma CD), alter the gut bacterial composition, it remains unclear whether cyclic oligosaccharides modify the IgA coating of gut bacteria, which relates to cyclic oligosaccharide-induced alteration of the gut bacterial composition. To address this issue, mice were maintained for 12 weeks on diets containing CNN, alpha CD, beta CD, or gamma CD; the animals' feces were evaluated for their bacterial composition and the IgA coating index (ICI), a measure of the degree of IgA coating of bacteria. We observed that the intake of each cyclic oligosaccharide altered the gut bacterial composition, with changes in the ICI found at both the phylum and genus levels. The ICI for Bacillota, Lachnospiraceae NK4A136 group, UC Lachnospiraceae, and Tuzzerella were significantly and positively correlated with the relative abundance (RA) in total bacteria for these bacteria; in contrast, significant correlations were not seen for other phyla and genera. Our observations suggest that cyclic oligosaccharide-induced modulation of the IgA coating of gut bacteria may partly relate to changes in the community structure of the gut bacteria.
en-copyright=
kn-copyright=
en-aut-name=MiyamotoTaisei
en-aut-sei=Miyamoto
en-aut-mei=Taisei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TeraokaMao
en-aut-sei=Teraoka
en-aut-mei=Mao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=WangTianyang
en-aut-sei=Wang
en-aut-mei=Tianyang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=5
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=cyclic oligosaccharides
kn-keyword=cyclic oligosaccharides
en-keyword=gut bacteria
kn-keyword=gut bacteria
en-keyword=immunoglobulin A
kn-keyword=immunoglobulin A
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=2400078
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240704
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Unabsorbed Fecal Fat Content Correlates with a Reduction of Immunoglobulin a Coating of Gut Bacteria in High‐Lard Diet‐Fed Mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Scope: Immunoglobulin A (IgA) selectively coats gut bacteria and contributes to regulatory functions in gastrointestinal inflammation and glucose metabolism. Excess intake of lard leads to decrease in the IgA coating of gut bacteria, although the underlying mechanisms remain unknown. This study validates how unabsorbed fat derived from a high-lard diet in the gut affects the IgA coating of bacteria, as assessed in mouse models using three types of dietary fat (lard, medium-, and long-chain triglycerides [MLCTs], and medium-chain triglycerides [MCTs]) exhibiting different digestibilities.
Methods and results: C57BL/6J mice are maintained on diets containing lard, MLCTs, or MCTs at 7% or 30% w/w for 10 weeks (n = 6 per group). The fecal fatty acid concentration is measured to quantify unabsorbed fat content. The ratio of IgA-coated bacteria to total bacteria (IgA coating ratio) in the feces is measured by flow cytometry. Compared to lard-fed mice, MLCT- and MCT-fed mice exhibit lower fecal concentrations of palmitic acid, stearic acid, and oleic acid and higher IgA coating ratios at both 7% and 30% dietary fat, and these parameters exhibit significant negative correlations.
Conclusion: Unabsorbed fat content in the gut may result in attenuated IgA coating of bacteria in high-lard diet-fed mice.
en-copyright=
kn-copyright=
en-aut-name=KatsumataEmiko
en-aut-sei=Katsumata
en-aut-mei=Emiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=SonoyamaKei
en-aut-sei=Sonoyama
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=YoshidaTakashi
en-aut-sei=Yoshida
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=SasakiMio
en-aut-sei=Sasaki
en-aut-mei=Mio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=TeraokaMao
en-aut-sei=Teraoka
en-aut-mei=Mao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=WangTianyang
en-aut-sei=Wang
en-aut-mei=Tianyang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=3
en-affil=Research Faculty of Agriculture, Hokkaido University
kn-affil=
affil-num=4
en-affil=TAIYO YUSHI Corporation
kn-affil=
affil-num=5
en-affil=TAIYO YUSHI Corporation
kn-affil=
affil-num=6
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=7
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=8
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=gut bacteria
kn-keyword=gut bacteria
en-keyword=immunoglobulin A
kn-keyword=immunoglobulin A
en-keyword=lard
kn-keyword=lard
END
start-ver=1.4
cd-journal=joma
no-vol=7
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210114
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cecum microbiota in rats fed soy, milk, meat, fish, and egg proteins with prebiotic oligosaccharides
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Diet is considered the most influential factor in modulating the gut microbiota but how dietary protein sources differ in their modulatory effects is not well understood. In this study, soy, meat (mixture of beef and pork), and fish proteins (experiment 1) and soy, milk (casein), and egg proteins (experiment 2) were fed to rats with cellulose (CEL) and raffinose (RAF); the microbiota composition and short-chain fatty acid concentration in the cecum were determined. Egg protein feeding decreased the concentration of acetic acid and the richness and diversity of the cecum microbiota. RAF feeding increased the concentrations of acetic and propionic acids and decreased the richness and diversity of the cecum microbiota. When fed with CEL, the abundance of Ruminococcaceae and Christensenellaceae, Akkermansiaceae and Tannerellaceae, and Erysipelotrichaceae enhanced with soy protein, meat and fish proteins, and egg protein, respectively. The effects of dietary proteins diminished with RAF feeding and the abundance of Bifidobacteriaceae, Erysipelotrichaceae, and Lachnospiraceae increased and that of Ruminococcaceae and Christensenellaceae decreased regardless of the protein source. These results indicate that, although the effect of prebiotics is more robust and distinctive, dietary protein sources may influence the composition and metabolic activities of the gut microbiota. The stimulatory effects of soy, meat, and egg proteins on Christensenellaceae, Akkermansiaceae, and Erysipelotrichaceae deserve further examination to better elucidate the dietary manipulation of the gut microbiota.
en-copyright=
kn-copyright=
en-aut-name=SivixaySouliphone
en-aut-sei=Sivixay
en-aut-mei=Souliphone
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=BaiGaowa
en-aut-sei=Bai
en-aut-mei=Gaowa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=Department of Animal Science, Graduate School of Life and Environmental Science, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Animal Science, Graduate School of Life and Environmental Science, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Animal Science, Graduate School of Life and Environmental Science, Okayama University
kn-affil=
affil-num=4
en-affil=Department of Animal Science, Graduate School of Life and Environmental Science, Okayama University
kn-affil=
en-keyword=diet
kn-keyword=diet
en-keyword=gut
kn-keyword=gut
en-keyword=microbiota
kn-keyword=microbiota
en-keyword=protein
kn-keyword=protein
en-keyword=prebiotics
kn-keyword=prebiotics
END
start-ver=1.4
cd-journal=joma
no-vol=39
cd-vols=
no-issue=3
article-no=
start-page=188
end-page=196
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=2020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cyclic nigerosylnigerose ameliorates DSS-induced colitis with restoration of goblet cell number and increase in IgA reactivity against gut microbiota in mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cyclic nigerosylnigerose (CNN) is a cyclic oligosaccharide. Oral administration of CNN promotes immunoglobulin A (IgA) secretion in the gut. IgA is a major antibody secreted into the gut and plays a crucial role in suppressing gut inflammation due to commensal gut microbiota. To investigate the effect of administration of CNN to promote IgA secretion on gut inflammation, experimental colitis was induced with dextran sulfate sodium (DSS) in Balb/c mice after 6 weeks of CNN pre-feeding. The severity of colitis was evaluated based on a disease activity index (DAI), the gene expression of inflammatory cytokines, and a histological examination. The CNN-treated mice with DSS-induced colitis (CNN-DSS group) showed significantly lower DAI scores and mRNA levels of interleukin-1 compared with the CNN-untreated mice with DSS-induced colitis (DSS group). Histological examination of the colon revealed that the pathological score was significantly lower in the CNN-DSS group compared with the DSS group due to the reduced infiltration of immune cells. The number of goblet cells was significantly higher in the CNN-DSS group compared with the DSS group. The IgA concentration and the ratio of microbiota coated with IgA were evaluated in the cecal content. Although there was no difference in the IgA concentration among groups, a higher proportion of cecal microbiota were coated with IgA in the CNN-DSS group compared with that in the DSS group. These results suggest that CNN might preserve goblet cells in the colon and promote IgA coating of gut microbiota, which synergistically ameliorate gut inflammation in mice with DSS-induced colitis.
en-copyright=
kn-copyright=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KatsumataEmiko
en-aut-sei=Katsumata
en-aut-mei=Emiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MizoteAkiko
en-aut-sei=Mizote
en-aut-mei=Akiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=JianHou Jian
en-aut-sei=Jian
en-aut-mei=Hou Jian
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=MuhomahTeresia Aluoch
en-aut-sei=Muhomah
en-aut-mei=Teresia Aluoch
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
affil-num=1
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=3
en-affil=Hayashibara Co., Ltd.
kn-affil=
affil-num=4
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=5
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=6
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=oligosaccharide
kn-keyword=oligosaccharide
en-keyword=DSS-induced colitis
kn-keyword=DSS-induced colitis
en-keyword=goblet cell
kn-keyword=goblet cell
en-keyword=gut microbiota
kn-keyword=gut microbiota
en-keyword=immunoglobulin A
kn-keyword=immunoglobulin A
END
start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=12
article-no=
start-page=1007
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20191121
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Relationship between Uterine, Fecal, Bedding, and Airborne Dust Microbiota from Dairy Cows and Their Environment: A Pilot Study
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Simple Summary After calving, dairy cows face the risk of negative energy balance, inflammation, and immunosuppression, which may result in bacterial infection and disruption of the normal microbiota, thus encouraging the development of metritis and endometritis. This study characterized uterine, fecal, bedding, and airborne dust microbiota from postpartum dairy cows and their environment during summer and winter. The results clarify the importance of microbiota in cowshed environments, i.e., bedding and airborne dust, in understanding the postpartum uterine microbiota of dairy cows.
Abstract The aim of this study was to characterize uterine, fecal, bedding, and airborne dust microbiota from postpartum dairy cows and their environment. The cows were managed by the free-stall housing system, and samples for microbiota and serum metabolite assessment were collected during summer and winter when the cows were at one and two months postpartum. Uterine microbiota varied between seasons; the five most prevalent taxa were Enterobacteriaceae, Moraxellaceae, Ruminococcaceae, Staphylococcaceae, and Lactobacillaceae during summer, and Ruminococcaceae, Lachnospiraceae, Bacteroidaceae, Moraxellaceae, and Clostridiaceae during winter. Although Actinomycetaceae and Mycoplasmataceae were detected at high abundance in several uterine samples, the relationship between the uterine microbiota and serum metabolite concentrations was unclear. The fecal microbiota was stable regardless of the season, whereas bedding and airborne dust microbiota varied between summer and winter. With regards to uterine, bedding, and airborne dust microbiota, Enterobacteriaceae, Moraxellaceae, Staphylococcaceae, and Lactobacillaceae were more abundant during summer, and Ruminococcaceae, Lachnospiraceae, Bacteroidaceae, and Clostridiaceae were more abundant during winter. Canonical analysis of principal coordinates confirmed the relationship between uterine and cowshed microbiota. These results indicated that the uterine microbiota may vary when the microbiota in cowshed environments changes.
en-copyright=
kn-copyright=
en-aut-name=NguyenThuong T.
en-aut-sei=Nguyen
en-aut-mei=Thuong T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MiyakeAyumi
en-aut-sei=Miyake
en-aut-mei=Ayumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TranTu T. M.
en-aut-sei=Tran
en-aut-mei=Tu T. M.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Okayama Prefecture Livestock Research Institute
kn-affil=
affil-num=3
en-affil=Faculty of Agriculture and Food Technology, Tien Giang University
kn-affil=
affil-num=4
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=5
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=cowshed
kn-keyword=cowshed
en-keyword=environment
kn-keyword=environment
en-keyword=microbiota
kn-keyword=microbiota
en-keyword=uterus
kn-keyword=uterus
END
start-ver=1.4
cd-journal=joma
no-vol=35
cd-vols=
no-issue=
article-no=
start-page=9
end-page=11
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=201906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Reactivity of secretory immunoglobulin A against gut bacteria is attenuated by high-fat diet consumption
kn-title=分泌型免疫グロブリンA の腸内細菌への結合活性は 高脂肪食の摂取により弱まる
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Secretory immunoglobulin A (SIgA) is predominant antibody secreted into the gut relating to maintain gut homeostasis. In the present study, we focused on the SIgA coating of gut bacteria as a mucosal immune response affecting the gut bacteria following a high-fat diet (HFD). This study newly showed that the level of SIgA coating of gut bacteria was significantly decreased in HFD-fed mice than normal-fat diet (NFD)-fed mice. Furthermore, we found that the relative abundances of gut bacteria were significantly negatively or positively correlated with the level of SIgA coating of gut bacteria in NFD-fed and HFD-fed mice. Our observations suggest that a decrease in the level of SIgA coating of the gut bacteria through a HFD might relate to HFD-induced changes in bacterial composition.
en-copyright=
kn-copyright=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=鶴田剛司
kn-aut-sei=鶴田
kn-aut-mei=剛司
aut-affil-num=1
ORCID=
en-aut-name=MUHOMAHTeresia Aluoch
en-aut-sei=MUHOMAH
en-aut-mei=Teresia Aluoch
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=SonoyamaKei
en-aut-sei=Sonoyama
en-aut-mei=Kei
kn-aut-name=園山慶
kn-aut-sei=園山
kn-aut-mei=慶
aut-affil-num=3
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=西野直樹
kn-aut-sei=西野
kn-aut-mei=直樹
aut-affil-num=4
ORCID=
affil-num=1
en-affil=Graduate school of Environmental and Life Sciences, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
affil-num=2
en-affil=Graduate school of Environmental and Life Sciences, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
affil-num=3
en-affil=Research Faculty of Agriculture, Hokkaido University
kn-affil=北海道大学大学院農学研究院
affil-num=4
en-affil=Graduate school of Environmental and Life Sciences, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
END
start-ver=1.4
cd-journal=joma
no-vol=34
cd-vols=
no-issue=
article-no=
start-page=32
end-page=35
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=201804
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Intracellular uptake of extracellular vesicles shed by bone marrow-derived macrophages stimulated with lactic acid bacteria in adipocyte and skeletal muscle cell line
kn-title=乳酸菌を貪食したマクロファージが放出する細胞外小胞の脂肪細胞 および骨格筋細胞への取り込み
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=鶴田剛司
kn-aut-sei=鶴田
kn-aut-mei=剛司
aut-affil-num=1
ORCID=
en-aut-name=ItoYohei
en-aut-sei=Ito
en-aut-mei=Yohei
kn-aut-name=伊藤洋平
kn-aut-sei=伊藤
kn-aut-mei=洋平
aut-affil-num=2
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=西野直樹
kn-aut-sei=西野
kn-aut-mei=直樹
aut-affil-num=3
ORCID=
en-aut-name=Teresia Aluoch Muhomah
en-aut-sei=Teresia Aluoch Muhomah
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=KatsumataEmiko
en-aut-sei=Katsumata
en-aut-mei=Emiko
kn-aut-name=勝又恵実子
kn-aut-sei=勝又
kn-aut-mei=恵実子
aut-affil-num=5
ORCID=
en-aut-name=SonoyamaKei
en-aut-sei=Sonoyama
en-aut-mei=Kei
kn-aut-name=園山慶
kn-aut-sei=園山
kn-aut-mei=慶
aut-affil-num=6
ORCID=
affil-num=1
en-affil=Okayama University Graduate School of Environmental and Life Science
kn-affil=岡山大学大学院環境生命科学研究科
affil-num=2
en-affil=Okayama University Graduate School of Environmental and Life Science
kn-affil=岡山大学大学院環境生命科学研究科
affil-num=3
en-affil=Okayama University Graduate School of Environmental and Life Science
kn-affil=岡山大学大学院環境生命科学研究科
affil-num=4
en-affil=Okayama University Graduate School of Environmental and Life Science
kn-affil=岡山大学大学院環境生命科学研究科
affil-num=5
en-affil=Okayama University Graduate School of Environmental and Life Science
kn-affil=岡山大学大学院環境生命科学研究科
affil-num=6
en-affil=Hokkaido University, Research Faculty of Agriculture
kn-affil=北海道大学大学院農学研究院
END
start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=35
end-page=39
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Identification of commensal bacteria coated with secretory immunoglobulin A
kn-title=分泌型免疫グロブリンＡが認識する 腸内細菌種の同定
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= A part of commensal intestinal bacteria in mammal including human, mouse, bovine and pig are coated with secretory immunoglobulin A (S?IgA). It has been suggested from our previous research that S?IgA coating of commensal bacteria occur in bacterial group specific manner in human and mouse intestine. Thus, identification of S?IgA?coated bacterial genera/species would certainly help to elucidate the interaction between S?IgA and commensal intestinal bacteria. However, the method to identify the genera/species of S?IgA?coated bacteria has not been established. To identify S?IgA?coated bacterial composition, we developed the method combining immunohistochemical detection of S?IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Furthermore, human and mice fecal S?IgA coated bacterial composition was evaluated by this newly developed method with ten frequently?used FISH probes. Fecal S?IgA?coated bacterial composition was successfully analyzed with this method and this analysis suggested that Enterobacteriaceae was preferably coated with S?IgA whereas Bacteroides/Prevotella and Lactobacillus/ Enterococcus groups seemed to be poorly coated with S?IgA. This method will be applied to confirm whether interaction between S?IgA and commensal intestinal bacteria relate to symptom of inflammatory bowel diseases.
en-copyright=
kn-copyright=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=鶴田剛司
kn-aut-sei=鶴田
kn-aut-mei=剛司
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学農学部
en-keyword=Secretory immunoglobulin A
kn-keyword=Secretory immunoglobulin A
en-keyword=commensal intestinal bacteria
kn-keyword=commensal intestinal bacteria
en-keyword=fluorescence in situ hybridization
kn-keyword=fluorescence in situ hybridization
END