ID | 62369 |
FullText URL | |
Author |
Hattori, Momoko
Institute of Plant Science and Resources, Okayama University
Takami, Tsuneaki
Institute of Plant Science and Resources, Okayama University
Himi, Eiko
Institute of Plant Science and Resources, Okayama University
Sakamoto, Wataru
IInstitute of Plant Science and Resources, Okayama University
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Abstract | The albino lemma 1 (alm1) mutants of barley (Hordeum vulgare L.) exhibit obvious chlorophyll-deficient hulls. Hulls are seed-enclosing tissues on the spike, consisting of the lemma and palea. The alm1 phenotype is also expressed in the pericarp, culm nodes and basal leaf sheaths, but leaf blades and awns are normal green. A single recessive nuclear gene controls tissue-specific alm1 phenotypic expression. Positional cloning revealed that the ALM1 gene encodes a Golden 2-like (GLK) transcription factor, HvGLK2, belonging to the GARP subfamily of Myb transcription factors. This finding was validated by genetic evidence indicating that all 10 alm1 mutants studied had a lesion in functionally important regions of HvGLK2, including the three alpha-helix domains, an AREAEAA motif and the GCT box. Transmission electron microscopy revealed that, in lemmas of the alm1.g mutant, the chloroplasts lacked thylakoid membranes, instead of stacked thylakoid grana in wild-type chloroplasts. Compared with wild type, alm1.g plants showed similar levels of leaf photosynthesis but reduced spike photosynthesis by 34%. The alm1.g mutant and the alm1.a mutant showed a reduction in 100-grain weight by 15.8% and 23.1%, respectively. As in other plants, barley has HvGLK2 and a paralog, HvGLK1. In flag leaves and awns, HvGLK2 and HvGLK1 are expressed at moderate levels, but in hulls, HvGLK1 expression was barely detectable compared with HvGLK2. Barley alm1/Hvglk2 mutants exhibit more severe phenotypes than glk2 mutants of other plant species reported to date. The severe alm1 phenotypic expression in multiple tissues indicates that HvGLK2 plays some roles that are nonredundant with HvGLK1.
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Keywords | chloroplast
GLK2
Hordeum vulgare
photosynthesis
spike
thylakoid
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Note | This is an Accepted Manuscript of an article published by Oxford University Press.
This fulltext is available in Jan. 2022. |
Published Date | 2021-1-13
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Publication Title |
Plant and Cell Physiology
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Volume | volume62
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Issue | issue3
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Publisher | Oxford University Press (OUP)
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Start Page | 447
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End Page | 457
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ISSN | 1471-9053
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2021 Japanese Society of Plant Physiologists
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File Version | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1093/pcp/pcab001
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Funder Name |
Japan Society for the Promotion of Science
Oohara Foundation
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助成番号 | 16K07556
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