start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=8
article-no=
start-page=1810
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200730
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Ligation of MHC Class II Induces PKC-Dependent Clathrin-Mediated Endocytosis of MHC Class II
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In addition to antigen presentation to CD4(+)T cells, aggregation of cell surface major histocompatibility complex class II (MHC-II) molecules induces signal transduction in antigen presenting cells that regulate cellular functions. We previously reported that crosslinking of MHC-II induced the endocytosis of MHC-II, which was associated with decreased surface expression levels in murine dendritic cells (DCs) and resulted in impaired activation of CD4(+)T cells. However, the downstream signal that induces MHC-II endocytosis remains to be elucidated. In this study, we found that the crosslinking of MHC-II induced intracellular Ca(2+)mobilization, which was necessary for crosslinking-induced MHC-II endocytosis. We also found that these events were suppressed by inhibitors of Syk and phospholipase C (PLC). Treatments with a phorbol ester promoted MHC-II endocytosis, whereas inhibitors of protein kinase C (PKC) suppressed crosslinking-induced endocytosis of MHC-II. These results suggest that PKC could be involved in this process. Furthermore, crosslinking-induced MHC-II endocytosis was suppressed by inhibitors of clathrin-dependent endocytosis. Our results indicate that the crosslinking of MHC-II could stimulate Ca(2+)mobilization and induce the clathrin-dependent endocytosis of MHC-II in murine DCs.
en-copyright=
kn-copyright=

en-aut-name=MasakiKento
en-aut-sei=Masaki
en-aut-mei=Kento
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HirakiYuhji
en-aut-sei=Hiraki
en-aut-mei=Yuhji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OnishiHiroka
en-aut-sei=Onishi
en-aut-mei=Hiroka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SatohYuka
en-aut-sei=Satoh
en-aut-mei=Yuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=RochePaul A.
en-aut-sei=Roche
en-aut-mei=Paul A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TanakaSatoshi
en-aut-sei=Tanaka
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=FurutaKazuyuki
en-aut-sei=Furuta
en-aut-mei=Kazuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Immunobiology, Faculty of Pharmacy and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Experimental Immunology Branch, National Cancer Institute, National Institutes of Health
kn-affil=

affil-num=6
en-affil=Department of Pharmacology, Division of Pathological Sciences, Kyoto Pharmaceutical University
kn-affil=

affil-num=7
en-affil=Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=MHC-II
kn-keyword=MHC-II
en-keyword=dendritic cells
kn-keyword=dendritic cells
en-keyword=endocytosis
kn-keyword=endocytosis
en-keyword=crosslink
kn-keyword=crosslink
en-keyword=PKC
kn-keyword=PKC
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=11-12
article-no=
start-page=1029
end-page=1034
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1988
dt-pub=1988
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A case of primary thoracic hemangiopericytoma: correlation between Ga-67 scintigraphic and pathological findings
kn-title=‹Ήo“ΰŒ΄”­‚Μhemangiopericytoma‚Μ1—α \(67)GaƒVƒ“ƒ`ƒOƒ‰ƒtƒB[‚Ζ•a—ŠŒ©‚Μ‘Ξ”δ\
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A 27-year-old male was admitted to our hospital with the chief complaint of dyspnea. Radiologically, he was found to have a large tumor shadow in the left hemithorax with pleural effusion. The first thoracotomy was done to resect the tumor on January 14, 1988. The second and third thoracotomies were done because of local recurrence in the left hemithorax. Histological diagnosis was of a hmangio-pericytoma. Before the third operation, Ga-67 scintigraphy was performed. Ga-67 accumulation was noted in parts of the tumor mass. Histological findings of the resected tumor were compared with the degree and location of Ga-67 accumulation. Histologically, Ga-67 accumulation occurred in areas of bleeding and necrosis in the tumor tissue, but not in viable parts of the tumor. Ga-67 accumulation usually occurs in viable tumor cells, but our findings were contrary to this generality.
en-copyright=
kn-copyright=

en-aut-name=TanabeMasatada
en-aut-sei=Tanabe
en-aut-mei=Masatada
kn-aut-name=“c糐³’‰
kn-aut-sei=“cη³
kn-aut-mei=³’‰
aut-affil-num=1
ORCID=

en-aut-name=GohdaFuminori
en-aut-sei=Gohda
en-aut-mei=Fuminori
kn-aut-name=‡“c•Ά‘₯
kn-aut-sei=‡“c
kn-aut-mei=•Ά‘₯
aut-affil-num=2
ORCID=

en-aut-name=YodenMidori
en-aut-sei=Yoden
en-aut-mei=Midori
kn-aut-name=—]“c‚έ‚Η‚θ
kn-aut-sei=—]“c
kn-aut-mei=‚έ‚Η‚θ
aut-affil-num=3
ORCID=

en-aut-name=SakamotoKazuhiro
en-aut-sei=Sakamoto
en-aut-mei=Kazuhiro
kn-aut-name=β–{˜a—T
kn-aut-sei=β–{
kn-aut-mei=˜a—T
aut-affil-num=4
ORCID=

en-aut-name=MiyamotoTsutomu
en-aut-sei=Miyamoto
en-aut-mei=Tsutomu
kn-aut-name=‹{–{•Χ
kn-aut-sei=‹{–{
kn-aut-mei=•Χ
aut-affil-num=5
ORCID=

en-aut-name=SeoHiroyuki
en-aut-sei=Seo
en-aut-mei=Hiroyuki
kn-aut-name=£”φ—T”V
kn-aut-sei=£”φ
kn-aut-mei=—T”V
aut-affil-num=6
ORCID=

en-aut-name=KawasakiYukiko
en-aut-sei=Kawasaki
en-aut-mei=Yukiko
kn-aut-name=μθKŽq
kn-aut-sei=μθ
kn-aut-mei=KŽq
aut-affil-num=7
ORCID=

en-aut-name=OhkawaSatoshi
en-aut-sei=Ohkawa
en-aut-mei=Satoshi
kn-aut-name=‘εμŒ³b
kn-aut-sei=‘εμ
kn-aut-mei=Œ³b
aut-affil-num=8
ORCID=

en-aut-name=TamaiToyosato
en-aut-sei=Tamai
en-aut-mei=Toyosato
kn-aut-name=‹Κˆδ–L—
kn-aut-sei=‹Κˆδ
kn-aut-mei=–L—
aut-affil-num=9
ORCID=

en-aut-name=YamamotoShinya
en-aut-sei=Yamamoto
en-aut-mei=Shinya
kn-aut-name=ŽR–{αΑ–η
kn-aut-sei=ŽR–{
kn-aut-mei=αΑ–η
aut-affil-num=10
ORCID=

en-aut-name=TanakaSatoshi
en-aut-sei=Tanaka
en-aut-mei=Satoshi
kn-aut-name=“c’†γΰ
kn-aut-sei=“c’†
kn-aut-mei=γΰ
aut-affil-num=11
ORCID=

en-aut-name=KobayashiShouji
en-aut-sei=Kobayashi
en-aut-mei=Shouji
kn-aut-name=¬—яȓρ
kn-aut-sei=¬—Ρ
kn-aut-mei=Θ“ρ
aut-affil-num=12
ORCID=

affil-num=1
en-affil=
kn-affil=μˆγ‰Θ‘εŠw•ϊŽΛόˆγŠw‹³ŽΊ

affil-num=2
en-affil=
kn-affil=μˆγ‰Θ‘εŠw•ϊŽΛόˆγŠw‹³ŽΊ

affil-num=3
en-affil=
kn-affil=μˆγ‰Θ‘εŠw•ϊŽΛόˆγŠw‹³ŽΊ

affil-num=4
en-affil=
kn-affil=μˆγ‰Θ‘εŠw•ϊŽΛόˆγŠw‹³ŽΊ

affil-num=5
en-affil=
kn-affil=μˆγ‰Θ‘εŠw•ϊŽΛόˆγŠw‹³ŽΊ

affil-num=6
en-affil=
kn-affil=μˆγ‰Θ‘εŠw•ϊŽΛόˆγŠw‹³ŽΊ

affil-num=7
en-affil=
kn-affil=μˆγ‰Θ‘εŠw•ϊŽΛόˆγŠw‹³ŽΊ

affil-num=8
en-affil=
kn-affil=μˆγ‰Θ‘εŠw•ϊŽΛόˆγŠw‹³ŽΊ

affil-num=9
en-affil=
kn-affil=μˆγ‰Θ‘εŠw•ϊŽΛόˆγŠw‹³ŽΊ

affil-num=10
en-affil=
kn-affil=μˆγ‰Θ‘εŠw‘ζˆκŠO‰Θ‹³ŽΊ

affil-num=11
en-affil=
kn-affil=μˆγ‰Θ‘εŠw‘ζˆκŠO‰Θ‹³ŽΊ

affil-num=12
en-affil=
kn-affil=μˆγ‰Θ‘εŠw‘ζˆκ•a—Šw‹³ŽΊ
en-keyword=‹Ήo“ΰŒ΄”­‚Μhemangiopericytoma
kn-keyword=‹Ήo“ΰŒ΄”­‚Μhemangiopericytoma
en-keyword=(67)GaƒVƒ“ƒ`ƒOƒ‰ƒtƒB[
kn-keyword=(67)GaƒVƒ“ƒ`ƒOƒ‰ƒtƒB[
END