start-ver=1.4
cd-journal=joma
no-vol=26
cd-vols=
no-issue=15
article-no=
start-page=7275
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250728
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Acquired Radioresistance Through Adaptive Evolution with Gamma Radiation as Selection Pressure: Increased Expression and Induction of Anti-Stress Genes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Elucidating the mechanisms of radioresistance in highly radiotolerant organisms can provide valuable insights into the adaptation and evolution of organisms. However, research has been limited on many naturally occurring radioresistant organisms due to a lack of information regarding their genetic and biochemical characteristics and the difficulty of handling them experimentally. To address this, we conducted an experiment on adaptive evolution using gamma radiation as the selection pressure to generate evolved Escherichia coli with gamma radiation resistance approximately one order of magnitude greater than that of wild-type E. coli. Gene expressions in all wild-type and evolved radioresistant E. coli in the presence or absence of gamma irradiation were analyzed and compared using RNA sequencing. Under steady-state conditions, the genes involved in survival, cell recovery, DNA repair, and response following stress exposure were upregulated in evolved E. coli compared with those in wild-type E. coli. Furthermore, the evolved E. coli induced these genes more efficiently following gamma irradiation and greater DNA repair activity than that in the wild-type E. coli. Our results indicate that an increased steady-state expression of various anti-stress genes, including DNA repair-related genes, and their highly efficient induction under irradiation are responsible for the remarkable radioresistance of evolved E. coli.
en-copyright=
kn-copyright=

en-aut-name=SaitoTakeshi
en-aut-sei=Saito
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TeratoHiroaki
en-aut-sei=Terato
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Division of Radiation Life Science, Institute for Integrated Radiation and Nuclear Science, Kyoto University
kn-affil=

affil-num=2
en-affil=Department of Radiation Research, Advanced Science Research Center, Okayama University
kn-affil=
en-keyword=radioresistant bacteria
kn-keyword=radioresistant bacteria
en-keyword=Escherichia coli
kn-keyword=Escherichia coli
en-keyword=adaptive evolution
kn-keyword=adaptive evolution
en-keyword=gene expression changes
kn-keyword=gene expression changes
en-keyword=anti-stress genes
kn-keyword=anti-stress genes
en-keyword=DNA repair
kn-keyword=DNA repair
en-keyword=cell recovery
kn-keyword=cell recovery
END

start-ver=1.4
cd-journal=joma
no-vol=184
cd-vols=
no-issue=
article-no=
start-page=43
end-page=51
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20231124
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Practical Study of Creative Learning in High School Music Classes Classes: Focusing on Structure of Melodies
kn-title=高等学校芸術科音楽における創作分野の実践的研究― 旋律の構造に着目して ―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　本研究は，高等学校芸術科音楽（以下，音楽Ⅰ）における創作学習を通して，創作に対する生徒の思考やその変化を捉えることを目的としている。創作の際は，音同士の細かな連結やその展開を示す「構造」という視点を基盤とし，間奏において表したいイメージと関わらせながら制作を進めた。生徒の作品からは，音程関係やリズムの繰り返し方に工夫を凝らした様子が見受けられ，微視的な構造と表現したいイメージとが強く結びついた作品が発表された。また，ワークシートやふりかえりフォームからは，困難な創作内容になるほど学習に興味をもっていたことがわかり，作曲の最も微視的視点である構造が，生徒の興味をもって深まっていたことが明らかとなった。
en-copyright=
kn-copyright=

en-aut-name=SAITOHTakeshi
en-aut-sei=SAITOH
en-aut-mei=Takeshi
kn-aut-name=齊藤武
kn-aut-sei=齊藤
kn-aut-mei=武
aut-affil-num=1
ORCID=

en-aut-name=TOKUDATeruaki
en-aut-sei=TOKUDA
en-aut-mei=Teruaki
kn-aut-name=徳田旭昭
kn-aut-sei=徳田
kn-aut-mei=旭昭
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Faculty of Education, Okayama University
kn-affil=岡山大学学術研究院教育学域

affil-num=2
en-affil=Okayama Sozan Senior High School
kn-affil=岡山県立岡山操山高等学校
en-keyword=創作学習
kn-keyword=創作学習
en-keyword=構造
kn-keyword=構造
en-keyword=高等学校
kn-keyword=高等学校
en-keyword=校歌
kn-keyword=校歌
en-keyword=間奏
kn-keyword=間奏
END