ID | 67252 |
Author |
Ikenaga, Takanori
Graduate School of Science and Engineering, Kagoshima University
Kobayashi, Aoshi
Ushimado Marine Institute (UMI), Faculty of Environmental, Life, Natural Science and Technology, Okayama University
Takeuchi, Akihisa
Japan Synchrotron Radiation Research Institute/SPring-8
Uesugi, Kentaro
Japan Synchrotron Radiation Research Institute/SPring-8
Maezawa, Takanobu
Department of Integrated Science and Technology, National Institute of Technology, Tsuyama College
Shibata, Norito
Department of Integrated Science and Technology, National Institute of Technology, Tsuyama College
Sakamoto, Tatsuya
Ushimado Marine Institute (UMI), Faculty of Environmental, Life, Natural Science and Technology, Okayama University
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Sakamoto, Hirotaka
Ushimado Marine Institute (UMI), Faculty of Environmental, Life, Natural Science and Technology, Okayama University
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Abstract | Platyhelminthes are a phylum of simple bilaterian invertebrates with prototypic body systems. Compared with non-bilaterians such as cnidarians, the bilaterians are likely to exhibit integrated free-moving behaviors, which require a concentrated nervous system “brain” rather than the distributed nervous system of radiatans. Marine flatworms have an early cephalized ‘central’ nervous system compared not only with non-bilaterians but also with parasitic flatworms or freshwater planarians. In this study, we used the marine flatworm Stylochoplana pusilla as an excellent model organism in Platyhelminthes because of the early cephalized central nervous system. Here, we investigated the three-dimensional structures of the flatworm central nervous system by the use of X-ray micro-computed tomography (micro-CT) in a synchrotron radiation facility. We found that the obtained tomographic images were sufficient to discriminate some characteristic structures of the nervous system, including nerve cords around the cephalic ganglion, mushroom body-like structures, and putative optic nerves forming an optic commissure-like structure. Through the micro-CT imaging, we could obtain undistorted serial section images, permitting us to visualize precise spatial relationships of neuronal subpopulations and nerve tracts. 3-D micro-CT is very effective in the volume analysis of the nervous system at the cellular level; the methodology is straightforward and could be applied to many other non-model organisms.
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Keywords | bilaterians
micro-CT scan
central nervous system
Platyhelminthes
marine flatworms
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Note | This fulltext file will be available in Apr. 2025.
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Published Date | 2024-04-08
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Publication Title |
Zoological Science
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Volume | volume41
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Issue | issue3
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Publisher | Zoological Society of Japan
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Start Page | 281
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End Page | 289
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ISSN | 0289-0003
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NCID | AA10545874
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2024 Zoological Society of Japan
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File Version | publisher
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PubMed ID | |
DOI | |
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Related Url | isVersionOf https://doi.org/10.2108/zs230082
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Citation | Takanori Ikenaga, Aoshi Kobayashi, Akihisa Takeuchi, Kentaro Uesugi, Takanobu Maezawa, Norito Shibata, Tatsuya Sakamoto, and Hirotaka Sakamoto "Volume X-Ray Micro-Computed Tomography Analysis of the Early Cephalized Central Nervous System in a Marine Flatworm, Stylochoplana pusilla," Zoological Science 41(3), 281-289, (8 April 2024). https://doi.org/10.2108/zs230082
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Funder Name |
Japan Society for the Promotion of Science
Ministry of Education, Culture, Sports, Science and Technology
Takeda Science Foundation
Suzuken Memorial Foundation
Wesco Scientific Promotion Foundation
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助成番号 | 17K07943
21K05772
20K21429
21H02520
22K19312
16H06280
20K06722
22H02656
22K19332
21H00428
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