start-ver=1.4
cd-journal=joma
no-vol=25
cd-vols=
no-issue=6
article-no=
start-page=3252
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240313
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Age-Related Effects on MSC Immunomodulation, Macrophage Polarization, Apoptosis, and Bone Regeneration Correlate with IL-38 Expression
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Mesenchymal stem cells (MSCs) are known to promote tissue regeneration and suppress excessive inflammation caused by infection or trauma. Reported evidence indicates that various factors influence the expression of MSCs' endogenous immunomodulatory properties. However, the detailed interactions of MSCs with macrophages, which are key cells involved in tissue repair, and their regulatory mechanisms are not completely understood. We herein investigated how age-related immunomodulatory impairment of MSCs alters the interaction of MSCs with macrophages during bone healing using young (5-week old) and aged (50-week old) mice. To clarify the relationship between inflammatory macrophages (M1) and MSCs, their spatiotemporal localization at the bone healing site was investigated by immunostaining, and possible regulatory mechanisms were analyzed in vitro co-cultures. Histomorphometric analysis revealed an accumulation of M1 and a decrease in MSC number at the healing site in aged mice, which showed a delayed bone healing. In in vitro co-cultures, MSCs induced M1 apoptosis through cell-to-cell contact but suppressed the gene expression of pro-inflammatory cytokines by soluble factors secreted in the culture supernatant. Interestingly, interleukin 38 (Il-38) expression was up-regulated in M1 after co-culture with MSCs. IL-38 suppressed the gene expression of inflammatory cytokines in M1 and promoted the expression of genes associated with M1 polarization to anti-inflammatory macrophages (M2). IL-38 also had an inhibitory effect on M1 apoptosis. These results suggest that MSCs may induce M1 apoptosis, suppress inflammatory cytokine production by M1, and induce their polarization toward M2. Nevertheless, in aged conditions, the decreased number and immunomodulatory function of MSCs could be associated with a delayed M1 clearance (i.e., apoptosis and/or polarization) and consequent delayed resolution of the inflammatory phase. Furthermore, M1-derived IL-38 may be associated with immunoregulation in the tissue regeneration site.
en-copyright=
kn-copyright=
en-aut-name=ZhangJiewen
en-aut-sei=Zhang
en-aut-mei=Jiewen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=AkiyamaKentaro
en-aut-sei=Akiyama
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MunAung Ye
en-aut-sei=Mun
en-aut-mei=Aung Ye
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=TagashiraRyuji
en-aut-sei=Tagashira
en-aut-mei=Ryuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=ZouTingling
en-aut-sei=Zou
en-aut-mei=Tingling
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=MatsunagaNaoya
en-aut-sei=Matsunaga
en-aut-mei=Naoya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=KohnoTeisaku
en-aut-sei=Kohno
en-aut-mei=Teisaku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=7
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=8
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=mesenchymal stem cell
kn-keyword=mesenchymal stem cell
en-keyword=aging
kn-keyword=aging
en-keyword=apoptosis
kn-keyword=apoptosis
en-keyword=cytokines
kn-keyword=cytokines
en-keyword=monocytes and macrophages
kn-keyword=monocytes and macrophages
en-keyword=immunomodulation
kn-keyword=immunomodulation
END
start-ver=1.4
cd-journal=joma
no-vol=19
cd-vols=
no-issue=3
article-no=
start-page=431
end-page=432
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201207
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=気管挿管における口腔内偶発症防止対策の必要性
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=縄稚久美子
kn-aut-sei=縄稚
kn-aut-mei=久美子
aut-affil-num=1
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=曽我賢彦
kn-aut-sei=曽我
kn-aut-mei=賢彦
aut-affil-num=2
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=山中玲子
kn-aut-sei=山中
kn-aut-mei=玲子
aut-affil-num=3
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=足羽孝子
kn-aut-sei=足羽
kn-aut-mei=孝子
aut-affil-num=4
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=伊藤真理
kn-aut-sei=伊藤
kn-aut-mei=真理
aut-affil-num=5
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=佐藤真千子
kn-aut-sei=佐藤
kn-aut-mei=真千子
aut-affil-num=6
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=窪木拓男
kn-aut-sei=窪木
kn-aut-mei=拓男
aut-affil-num=7
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=森田潔
kn-aut-sei=森田
kn-aut-mei=潔
aut-affil-num=8
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学病院周術期管理センター
affil-num=2
en-affil=
kn-affil=岡山大学病院周術期管理センター
affil-num=3
en-affil=
kn-affil=岡山大学病院周術期管理センター
affil-num=4
en-affil=
kn-affil=岡山大学病院周術期管理センター
affil-num=5
en-affil=
kn-affil=岡山大学病院周術期管理センター
affil-num=6
en-affil=
kn-affil=岡山大学病院周術期管理センター
affil-num=7
en-affil=
kn-affil=岡山大学病院クラウンブリッジ補綴科
affil-num=8
en-affil=
kn-affil=岡山大学病院周術期管理センター
en-keyword=dental injury
kn-keyword=dental injury
en-keyword=perioperative management
kn-keyword=perioperative management
en-keyword=complication
kn-keyword=complication
END
start-ver=1.4
cd-journal=joma
no-vol=2016
cd-vols=
no-issue=
article-no=
start-page=7530942
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160612
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cellular Reprogramming Using Defined Factors and MicroRNAs
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Development of human bodies, organs, and tissues contains numerous steps of cellular differentiation including an initial zygote, embryonic stem (ES) cells, three germ layers, and multiple expertized lineages of cells. Induced pluripotent stem (iPS) cells have been recently developed using defined reprogramming factors such as Nanog, Klf5, Oct3/4 (Pou5f1), Sox2, and Myc. This outstanding innovation is largely changing life science and medicine. Methods of direct reprogramming of cells into myocytes, neurons, chondrocytes, and osteoblasts have been further developed using modified combination of factors such as N-myc, L-myc, Sox9, and microRNAs in defined cell/tissue culture conditions. Mesenchymal stem cells (MSCs) and dental pulp stem cells (DPSCs) are also emerging multipotent stem cells with particular microRNA expression signatures. It was shown that miRNA-720 had a role in cellular reprogramming through targeting the pluripotency factor Nanog and induction of DNA methyltransferases (DNMTs). This review reports histories, topics, and idea of cellular reprogramming.
en-copyright=
kn-copyright=
en-aut-name=EguchiTakanori
en-aut-sei=Eguchi
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
affil-num=1
en-affil=Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine
kn-affil=
END
start-ver=1.4
cd-journal=joma
no-vol=63
cd-vols=
no-issue=1
article-no=
start-page=125
end-page=129
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190131
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Digital transfer of the subgingival contour and emergence profile of the provisional restoration to the final bone-anchored fixed restoration
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=PURPOSE:
This report was written to introduce an attempt at clinical application of our newly developed digital workflow to reproduce the morphology of the subgingival contour and the emergence profile of the provisional restoration within the final bone-anchored fixed restoration, using a bounded unilateral edentulous case.
METHODS:
This digital workflow involves superimposition of the composite images of two specific types of working casts onto the working cast for the provisional restoration namely, a split cast screwed with a titanium base and a split cast screwed with a provisional restoration and integrating these with the whole intraoral surface image, in which the provisional restoration was present. The final restoration fabricated using this technique could be installed without any clinical problems. The results of in silico analysis revealed that the cubic volume ratio of the total discrepancy between the provisional and the final restorations was only 2.4%. Further, sufficient oral hygiene was maintained and the patient was satisfied with the outcome of the treatment.
CONCLUSIONS:
This technical report suggests that our newly developed digital workflow provided clinical applicability and may enable accurate transfer of the morphology of the subgingival contour and emergence profile of the provisional to the final bone-anchored fixed restoration.
en-copyright=
kn-copyright=
en-aut-name=KurosakiYoko
en-aut-sei=Kurosaki
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MinoTakuya
en-aut-sei=Mino
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MaekawaKenji
en-aut-sei=Maekawa
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=IzumiKoji
en-aut-sei=Izumi
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=SHIKEN Corporation
kn-affil=
affil-num=5
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=Digital transfer
kn-keyword=Digital transfer
en-keyword=Emergence profile
kn-keyword=Emergence profile
en-keyword=Implant fixed restoration
kn-keyword=Implant fixed restoration
en-keyword=In silico analysis
kn-keyword=In silico analysis
en-keyword=Subgingival contour
kn-keyword=Subgingival contour
END
start-ver=1.4
cd-journal=joma
no-vol=47
cd-vols=
no-issue=3
article-no=
start-page=281
end-page=288
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20191119
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Association of masticatory muscle activity with sleep arousal and other concomitant movements during sleep
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=OBJECTIVE:
This study aims to verify the associations among sleep bruxism (SB), sleep arousal (SA) and concurrent body movements.
MATERIAL AND METHODS:
Subjects underwent a standard overnight polysomnography test and audio-video recordings. Sleep quality was evaluated according to the Rechtschaffen and Kales criteria, while SA was determined as per the American Sleep Disorders Association criteria. Analyses were performed by an external institution after masking of the subjects' information. SB was assessed based on the presence/absence of rhythmic masticatory muscle activity (RMMA) episodes, which were identified by using electromyography of the masseter muscle. The observed simultaneous movements included lower leg movement (LLM), swallowing, face scratching, head movement, body movement, eye blinking, coughing, licking, sighing, body scratching, lip sucking, somniloquy and yawning. The LLM was determined visually, as well as through an increase in the tibialis electromyogram signal. Other movements were visually assessed using audio-video recordings. The incidences of all the simultaneous movements were compared between RMMA with intercurrent SA (SAwRMMA; RMMA episode derived from a masseter electromyogram showing more than 10% of maximum voluntary contraction) and SA without RMMA (SAw/oRMMA).
RESULTS:
Fourteen subjects were included in this study (females/males: 4/10, mean age: 31.5 ± 5.7 years). Among these, LLM, swallowing, body movement, licking, body scratching and lip sucking were frequently observed in SAwRMMA episodes than in SAw/oRMMA episodes, significantly. However, the non-specific simultaneous movements were higher observed in SAw/oRMMA episodes than that in SAwRMMA.
CONCLUSION:
Our results suggest that SB is concurrently activated with LLM in relation to arousal.
en-copyright=
kn-copyright=
en-aut-name=MikiHaruna
en-aut-sei=Miki
en-aut-mei=Haruna
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MinakuchiHajime
en-aut-sei=Minakuchi
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MiyagiMayu
en-aut-sei=Miyagi
en-aut-mei=Mayu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=HaraEmilio Satoshi
en-aut-sei=Hara
en-aut-mei=Emilio Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=ShigemotoShuji
en-aut-sei=Shigemoto
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=SuzukiYoshitaka
en-aut-sei=Suzuki
en-aut-mei=Yoshitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MaekawaKenji
en-aut-sei=Maekawa
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=MatsukaYoshizo
en-aut-sei=Matsuka
en-aut-mei=Yoshizo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=ClarkGlenn T.
en-aut-sei=Clark
en-aut-mei=Glenn T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Stomatgnathic Function and Occlusal Reconstruction, Institute of Biomedical Sciences, Clinical Dentistry, Tokushima University Graduate School
kn-affil=
affil-num=4
en-affil=Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Fixed Prosthodontics, School of Dental Medicine, Tsurumi University
kn-affil=
affil-num=6
en-affil=Department of Stomatgnathic Function and Occlusal Reconstruction, Institute of Biomedical Sciences, Clinical Dentistry, Tokushima University Graduate School
kn-affil=
affil-num=7
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=8
en-affil=Department of Stomatgnathic Function and Occlusal Reconstruction, Institute of Biomedical Sciences, Clinical Dentistry, Tokushima University Graduate School
kn-affil=
affil-num=9
en-affil=Advanced Program in Orofacial Pain and Oral Medicine, Ostrow School of Dentistry, University of Southern California
kn-affil=
affil-num=10
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=concomitant movement
kn-keyword=concomitant movement
en-keyword=non-specific simultaneous movements
kn-keyword=non-specific simultaneous movements
en-keyword=polysomnography assessment
kn-keyword=polysomnography assessment
en-keyword=rhythmic masticatory muscle activity
kn-keyword=rhythmic masticatory muscle activity
en-keyword=sleep arousal
kn-keyword=sleep arousal
en-keyword=sleep bruxism
kn-keyword=sleep bruxism
END
start-ver=1.4
cd-journal=joma
no-vol=21
cd-vols=
no-issue=21
article-no=
start-page=8103
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20201030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Mesenchymal stem cells (MSCs) are known to play important roles in the repair of lost or damaged tissues and immunotolerance. On the other hand, aging is known to impair MSC function. However, little is currently known about how aged MSCs affect the host response to the local inflammatory condition and tissue deterioration in periodontitis, which is a progressive destructive disease of the periodontal tissue potentially leading to multiple tooth loss. In this study, we examined the relationship between aging-induced impairment of MSC function and the severity of periodontal tissue destruction associated with the decrease in host immunomodulatory response using a ligature-induced periodontitis model in young and aged mice. The results of micro computerized tomography (micro-CT) and histological analysis revealed a more severe bone loss associated with increased osteoclast activity in aged (50-week-old) mice compared to young (5-week-old) mice. Immunostaining analysis revealed that, in aged mice, the accumulation of inflammatory T and B cells was higher, whereas the percentage of platelet-derived growth factor receptor alpha (PDGFR alpha)(+) MSCs, which are known to modulate the apoptosis of T cells, was significantly lower than in young mice. In vitro analysis of MSC function showed that the expression of surface antigen markers for MSCs (Sca-1, CD90, CD146), colony formation, migration, and osteogenic differentiation of aged MSCs were significantly declined compared to those of young MSCs. Moreover, a significantly higher proportion of aged MSCs were positive for the senescence-associated beta galactosidase activity. Importantly, aged MSCs presented a decreased expression of FAS-L, which was associated with a lower immunomodulatory property of aged MSCs to induce T cell apoptosis in co-cultures compared with young MSCs. In summary, this is the first study showing that aging-induced impairment of MSC function, including immunomodulatory response, is potentially correlated with progressive periodontal tissue deterioration.
en-copyright=
kn-copyright=
en-aut-name=AungKyaw Thu
en-aut-sei=Aung
en-aut-mei=Kyaw Thu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=AkiyamaKentaro
en-aut-sei=Akiyama
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=KunitomoMasayoshi
en-aut-sei=Kunitomo
en-aut-mei=Masayoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MunAung Ye
en-aut-sei=Mun
en-aut-mei=Aung Ye
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=TosaIkue
en-aut-sei=Tosa
en-aut-mei=Ikue
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=NguyenHa Thi Thu
en-aut-sei=Nguyen
en-aut-mei=Ha Thi Thu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=ZhangJiewen
en-aut-sei=Zhang
en-aut-mei=Jiewen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=KohnoTeisaku
en-aut-sei=Kohno
en-aut-mei=Teisaku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=OnoMitsuaki
en-aut-sei=Ono
en-aut-mei=Mitsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=HaraEmilio Satoshi
en-aut-sei=Hara
en-aut-mei=Emilio Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
affil-num=1
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=7
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=8
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=9
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=10
en-affil=Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=11
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=mesenchymal stem cell
kn-keyword=mesenchymal stem cell
en-keyword=aging
kn-keyword=aging
en-keyword=tissue destruction
kn-keyword=tissue destruction
en-keyword=periodontitis
kn-keyword=periodontitis
en-keyword=immunomodulation
kn-keyword=immunomodulation
en-keyword=bone resorption
kn-keyword=bone resorption
END
start-ver=1.4
cd-journal=joma
no-vol=21
cd-vols=
no-issue=19
article-no=
start-page=7028
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200924
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=BMP-2/beta-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Medication-related osteonecrosis of the jaw (MRONJ) is a severe pathological condition associated mainly with the long-term administration of bone resorption inhibitors, which are known to induce suppression of osteoclast activity and bone remodeling. Bone Morphogenetic Protein (BMP)-2 is known to be a strong inducer of bone remodeling, by directly regulating osteoblast differentiation and osteoclast activity. This study aimed to evaluate the effects of BMP-2 adsorbed onto beta-tricalcium phosphate (beta-TCP), which is an osteoinductive bioceramic material and allows space retention, on the prevention and treatment of MRONJ in mice. Tooth extraction was performed after 3 weeks of zoledronate (ZA) and cyclophosphamide (CY) administration. For prevention studies, BMP-2/beta-TCP was transplanted immediately after tooth extraction, and the mice were administered ZA and CY for an additional 4 weeks. The results showed that while the tooth extraction socket was mainly filled with a sparse tissue in the control group, bone formation was observed at the apex of the tooth extraction socket and was filled with a dense connective tissue rich in cellular components in the BMP-2/beta-TCP transplanted group. For treatment studies, BMP-2/beta-TCP was transplanted 2 weeks after tooth extraction, and bone formation was followed up for the subsequent 4 weeks under ZA and CY suspension. The results showed that although the tooth extraction socket was mainly filled with soft tissue in the control group, transplantation of BMP-2/beta-TCP could significantly accelerate bone formation, as shown by immunohistochemical analysis for osteopontin, and reduce the bone necrosis in tooth extraction sockets. These data suggest that the combination of BMP-2/beta-TCP could become a suitable therapy for the management of MRONJ.
en-copyright=
kn-copyright=
en-aut-name=MikaiAkihiro
en-aut-sei=Mikai
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=OnoMitsuaki
en-aut-sei=Ono
en-aut-mei=Mitsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TosaIkue
en-aut-sei=Tosa
en-aut-mei=Ikue
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=Ha Thi ThuNguyen
en-aut-sei=Ha Thi Thu
en-aut-mei=Nguyen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=HaraEmilio Satoshi
en-aut-sei=Hara
en-aut-mei=Emilio Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=NoshoShuji
en-aut-sei=Nosho
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=Kimura-OnoAya
en-aut-sei=Kimura-Ono
en-aut-mei=Aya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=NawachiKumiko
en-aut-sei=Nawachi
en-aut-mei=Kumiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=TakaradaTakeshi
en-aut-sei=Takarada
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=OohashiToshitaka
en-aut-sei=Oohashi
en-aut-mei=Toshitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
affil-num=1
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=7
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=8
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=9
en-affil=Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=10
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=11
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=BMP-2
kn-keyword=BMP-2
en-keyword=MRONJ
kn-keyword=MRONJ
en-keyword=bone regeneration
kn-keyword=bone regeneration
END
start-ver=1.4
cd-journal=joma
no-vol=21
cd-vols=
no-issue=21
article-no=
start-page=7967
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20201027
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2) have been regarded as the major cytokines promoting bone formation, however, several studies have reported unexpected results with failure of bone formation or bone resorption of these growth factors. In this study, BMP-2 and FGF-2 adsorbed into atellocollagen sponges were transplanted into bone defects in the bone marrow-scarce calvaria (extramedullary environment) and bone marrow-abundant femur (medullary environment) for analysis of their in vivo effects not only on osteoblasts, osteoclasts but also on bone marrow cells. The results showed that BMP-2 induced high bone formation in the bone marrow-scarce calvaria, but induced bone resorption in the bone marrow-abundant femurs. On the other hand, FGF-2 showed opposite effects compared to those of BMP-2. Analysis of cellular dynamics revealed numerous osteoblasts and osteoclasts present in the newly-formed bone induced by BMP-2 in calvaria, but none were seen in either control or FGF-2-transplanted groups. On the other hand, in the femur, numerous osteoclasts were observed in the vicinity of the BMP-2 pellet, while a great number of osteoblasts were seen near the FGF-2 pellets or in the control group. Of note, FCM analysis showed that both BMP-2 and FGF-2 administrated in the femur did not significantly affect the hematopoietic cell population, indicating a relatively safe application of the two growth factors. Together, these results indicate that BMP-2 could be suitable for application in extramedullary bone regeneration, whereas FGF-2 could be suitable for application in medullary bone regeneration.
en-copyright=
kn-copyright=
en-aut-name=NoshoShuji
en-aut-sei=Nosho
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TosaIkue
en-aut-sei=Tosa
en-aut-mei=Ikue
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=OnoMitsuaki
en-aut-sei=Ono
en-aut-mei=Mitsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=HaraEmilio Satoshi
en-aut-sei=Hara
en-aut-mei=Emilio Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=IshibashiKei
en-aut-sei=Ishibashi
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=MikaiAkihiro
en-aut-sei=Mikai
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=TanakaYukie
en-aut-sei=Tanaka
en-aut-mei=Yukie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=Kimura-OnoAya
en-aut-sei=Kimura-Ono
en-aut-mei=Aya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=KomoriTaishi
en-aut-sei=Komori
en-aut-mei=Taishi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=MaekawaKenji
en-aut-sei=Maekawa
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=OohashiToshitaka
en-aut-sei=Oohashi
en-aut-mei=Toshitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
affil-num=1
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=7
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=8
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=9
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=10
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=11
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=12
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=BMP-2
kn-keyword=BMP-2
en-keyword=FGF-2
kn-keyword=FGF-2
en-keyword=bone formation
kn-keyword=bone formation
en-keyword=bone marrow
kn-keyword=bone marrow
END
start-ver=1.4
cd-journal=joma
no-vol=20
cd-vols=
no-issue=6
article-no=
start-page=607
end-page=614
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200614
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Number of functional teeth more strongly predicts all‐cause mortality than number of present teeth in Japanese older adults
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Aim
Previous studies on the association between intraoral conditions and mortality in community‐dwelling older individuals reported that fewer present teeth (PT) are significant risk factors for mortality. However, how the number of PT relative to the number of functional teeth (FT), including both present and rehabilitated teeth, influences mortality has not been investigated fully. This study examined the impact of the number of FT on mortality among community‐dwelling Japanese older adults.
Methods
This study was a retrospective, observational and population‐based follow‐up study, which examined 1188 older individuals who participated in an annual geriatric health examination from 2009 to 2015. The average follow‐up period was 1697.0?±?774.5?days. The primary outcome was all‐cause mortality at follow‐up. The numbers of PT and FT of each participant were counted during an oral examination. In addition, demographics, clinical variables, blood nutrient markers, physical functions and perceived masticatory function were measured.
Results
Kaplan?Meier analysis, followed by a log‐rank test, revealed that fewer PT (P?0.001) and FT (P = 0.002) were significantly associated with a reduced survival rate. Cox's proportional hazard analysis indicated that the number of FT, but not the number of PT, was a significant independent mortality risk factor after adjusting for demographics, clinical variables, nutrient markers and physical functioning (P = 0.036, hazard ratio: 2.089).
Conclusions
Current results suggest that the number of FT more strongly predicts all‐cause mortality than the number of PT among community‐dwelling older adults. Further studies are necessary to consider the confounding of socioeconomic status and disability status.
en-copyright=
kn-copyright=
en-aut-name=MaekawaKenji
en-aut-sei=Maekawa
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=IkeuchiTomoko
en-aut-sei=Ikeuchi
en-aut-mei=Tomoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ShinkaiShoji
en-aut-sei=Shinkai
en-aut-mei=Shoji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=HiranoHirohiko
en-aut-sei=Hirano
en-aut-mei=Hirohiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=RyuMasahiro
en-aut-sei=Ryu
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=TamakiKatsushi
en-aut-sei=Tamaki
en-aut-mei=Katsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=YataniHirofumi
en-aut-sei=Yatani
en-aut-mei=Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=Kusatsu ISLE Study Working Group Collaborators
en-aut-sei=Kusatsu ISLE Study Working Group Collaborators
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=Kimura‐Ono Aya
en-aut-sei=Kimura‐Ono
en-aut-mei=Aya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=KikutanTakeshi
en-aut-sei=Kikutan
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=SuganumaTakashi
en-aut-sei=Suganuma
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=AyukawaYasunori
en-aut-sei=Ayukawa
en-aut-mei=Yasunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
en-aut-name=GondaTomoya
en-aut-sei=Gonda
en-aut-mei=Tomoya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=
en-aut-name=OgawaToru
en-aut-sei=Ogawa
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=
en-aut-name=FujisawaMasanori
en-aut-sei=Fujisawa
en-aut-mei=Masanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=
en-aut-name=Ishigaki Shoichi
en-aut-sei=Ishigaki
en-aut-mei=Shoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=
en-aut-name=WatanabeYutaka
en-aut-sei=Watanabe
en-aut-mei=Yutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=
en-aut-name=KitamuraAkihiko
en-aut-sei=Kitamura
en-aut-mei=Akihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=
en-aut-name=Taniguchi Yu
en-aut-sei=Taniguchi
en-aut-mei=Yu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=
en-aut-name=FujiwaraYoshinori
en-aut-sei=Fujiwara
en-aut-mei=Yoshinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=
en-aut-name=EdahiroAyako
en-aut-sei=Edahiro
en-aut-mei=Ayako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=
en-aut-name=Ohara Yuki
en-aut-sei=Ohara
en-aut-mei= Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=23
ORCID=
en-aut-name=FuruyaJunichi
en-aut-sei=Furuya
en-aut-mei=Junichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=24
ORCID=
en-aut-name=NakajimaJunko
en-aut-sei=Nakajima
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=25
ORCID=
en-aut-name=UmekiKento
en-aut-sei=Umeki
en-aut-mei=Kento
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=26
ORCID=
en-aut-name=IgarashiKentaro
en-aut-sei=Igarashi
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=27
ORCID=
en-aut-name=Horibeasuhiro
en-aut-sei=Horibe
en-aut-mei=asuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=28
ORCID=
en-aut-name=KugimiyaYoshihiro
en-aut-sei=Kugimiya
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=29
ORCID=
en-aut-name=KawaiYasuhiko
en-aut-sei=Kawai
en-aut-mei=Yasuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=30
ORCID=
en-aut-name=MatsumuraHideo
en-aut-sei=Matsumura
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=31
ORCID=
en-aut-name=IchikawaTetsuo
en-aut-sei=Ichikawa
en-aut-mei=Tetsuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=32
ORCID=
en-aut-name=Ohkawa Shuji
en-aut-sei=Ohkawa
en-aut-mei= Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=33
ORCID=
affil-num=1
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=3
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=4
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=5
en-affil=Research Planning and Promotion Committee
kn-affil=
affil-num=6
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=7
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=8
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=9
en-affil=
kn-affil=
affil-num=10
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=11
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=12
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=13
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=14
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=15
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=16
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=17
en-affil=Research Planning and Promotion Committee, Japan Prosthodontic Society
kn-affil=
affil-num=18
en-affil=Hokkaido University Faculty of Dental Medicine
kn-affil=
affil-num=19
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=20
en-affil=National Institute for Environmental Studies
kn-affil=
affil-num=21
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=22
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=23
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=24
en-affil=Tokyo Medical and Dental University
kn-affil=
affil-num=25
en-affil=Tokyo Dental College
kn-affil=
affil-num=26
en-affil=Nihon University School of Dentistry at Matsudo
kn-affil=
affil-num=27
en-affil=Nihon University School of Dentistry at Matsudo
kn-affil=
affil-num=28
en-affil=Tokyo Dental College
kn-affil=
affil-num=29
en-affil=Tokyo Dental College
kn-affil=
affil-num=30
en-affil=Nihon University School of Dentistry at Matsudo
kn-affil=
affil-num=31
en-affil=Nihon University School of Dentistry
kn-affil=
affil-num=32
en-affil=Tokushima University Graduate School, Institute of Biomedical Sciences
kn-affil=
affil-num=33
en-affil=Meikai University School of Dentistry
kn-affil=
en-keyword=functional teeth
kn-keyword=functional teeth
en-keyword=mortality
kn-keyword=mortality
en-keyword=present teeth
kn-keyword=present teeth
en-keyword=risk factor
kn-keyword=risk factor
END
start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=1
article-no=
start-page=208
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210104
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Tryptophan and Kynurenine Enhances the Stemness and Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro and In Vivo
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Aging tissues present a progressive decline in homeostasis and regenerative capacities, which has been associated with degenerative changes in tissue-specific stem cells and stem cell niches. We hypothesized that amino acids could regulate the stem cell phenotype and differentiation ability of human bone marrow-derived mesenchymal stromal cells (hBMSCs). Thus, we performed a screening of 22 standard amino acids and found that D-tryptophan (10 mu M) increased the number of cells positive for the early stem cell marker SSEA-4, and the gene expression levels of OCT-4, NANOG, and SOX-2 in hBMSCs. Comparison between D- and L-tryptophan isomers showed that the latter presents a stronger effect in inducing the mRNA levels of Oct-4 and Nanog, and in increasing the osteogenic differentiation of hBMSCs. On the other hand, L-tryptophan suppressed adipogenesis. The migration and colony-forming ability of hBMSCs were also enhanced by L-tryptophan treatment. In vivo experiments delivering L-tryptophan (50 mg/kg/day) by intraperitoneal injections for three weeks confirmed that L-tryptophan significantly increased the percentage of cells positive for SSEA-4, mRNA levels of Nanog and Oct-4, and the migration and colony-forming ability of mouse BMSCs. L-kynurenine, a major metabolite of L-tryptophan, also induced similar effects of L-tryptophan in enhancing stemness and osteogenic differentiation of BMSCs in vitro and in vivo, possibly indicating the involvement of the kynurenine pathway as the downstream signaling of L-tryptophan. Finally, since BMSCs migrate to the wound healing site to promote bone healing, surgical defects of 1 mm in diameter were created in mouse femur to evaluate bone formation after two weeks of L-tryptophan or L-kynurenine injection. Both L-tryptophan and L-kynurenine accelerated bone healing compared to the PBS-injected control group. In summary, L-tryptophan enhanced the stemness and osteoblastic differentiation of BMSCs and may be used as an essential factor to maintain the stem cell properties and accelerate bone healing and/or prevent bone loss.
en-copyright=
kn-copyright=
en-aut-name=PhamHai Thanh
en-aut-sei=Pham
en-aut-mei=Hai Thanh
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=OnoMitsuaki
en-aut-sei=Ono
en-aut-mei=Mitsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=HaraEmilio Satoshi
en-aut-sei=Hara
en-aut-mei=Emilio Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=NguyenHa Thi Thu
en-aut-sei=Nguyen
en-aut-mei=Ha Thi Thu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=DangAnh Tuan
en-aut-sei=Dang
en-aut-mei=Anh Tuan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=DoHang Thuy
en-aut-sei=Do
en-aut-mei=Hang Thuy
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=KomoriTaishi
en-aut-sei=Komori
en-aut-mei=Taishi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=TosaIkue
en-aut-sei=Tosa
en-aut-mei=Ikue
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=Hazehara-KunitomoYuri
en-aut-sei=Hazehara-Kunitomo
en-aut-mei=Yuri
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=YoshiokaYuya
en-aut-sei=Yoshioka
en-aut-mei=Yuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=OidaYasutaka
en-aut-sei=Oida
en-aut-mei=Yasutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=AkiyamaKentaro
en-aut-sei=Akiyama
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
affil-num=1
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=7
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=8
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=9
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=10
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=11
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=12
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=13
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=amino acid
kn-keyword=amino acid
en-keyword=mesenchymal stromal cells
kn-keyword=mesenchymal stromal cells
en-keyword=stemness
kn-keyword=stemness
en-keyword=tryptophan
kn-keyword=tryptophan
en-keyword=kynurenine
kn-keyword=kynurenine
en-keyword=osteogenesis
kn-keyword=osteogenesis
en-keyword=adipogenesis
kn-keyword=adipogenesis
en-keyword=screening
kn-keyword=screening
en-keyword=injury/fracture healing
kn-keyword=injury/fracture healing
en-keyword=anabolics
kn-keyword=anabolics
END
start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=1
article-no=
start-page=380
end-page=388
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210131
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A comparison of colorimetric and visual methods for the assessment of masticatory performance with color-changeable chewing gum in older persons
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background/purpose
Color-changeable chewing gum is used for the evaluation of masticatory performance. However, it is currently unclear whether colorimetric and visual assessment methods yield consistent results. This study aimed to clarify the consistency between colorimetric and visual methods used for the evaluation of color changes in color-changeable chewing gum.
Materials and methods
The sample comprised 644 older persons (mean age, 75.4?±?6.4 years). The chewing gum was masticated 60 times at the participant's own chewing rate and then expectorated. The color of the chewing gum was evaluated with the ΔE values and a? values, measured using a colorimeter, and the 10 Color Shades (10CSh) and 5 Color Scales (5CSc), using visual evaluation. Spearman's correlation analysis was performed to examine the correlation between the results obtained by the four methods. The significance level was set at α?=?0.05.
Results
The ΔE values, a? values, 10CSh scores, and 5CSc scores were all significantly correlated. The highest correlation coefficient (0.979) was between the ΔE values and a? values. The lowest correlation coefficient (0.847) was between the a? values and 5CSc scores. Decreased masticatory performance was observed with increased age.
Conclusion
Significant correlations were found for all four methods used in the assessment of masticatory performance with color-changeable chewing gum. While visually based assessments are valid, colorimetric methods are more sensitive to smaller changes in masticatory performance.
en-copyright=
kn-copyright=
en-aut-name=KugimiyaYoshihiro
en-aut-sei=Kugimiya
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=WatanabeYutaka
en-aut-sei=Watanabe
en-aut-mei=Yutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ShirobeMaki
en-aut-sei=Shirobe
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MotohashiYoshiko
en-aut-sei=Motohashi
en-aut-mei=Yoshiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=MotokawaKeiko
en-aut-sei=Motokawa
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=EdahiroAyako
en-aut-sei=Edahiro
en-aut-mei=Ayako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=OharaYuki
en-aut-sei=Ohara
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=RyuMasahiro
en-aut-sei=Ryu
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=IgarashiKentaro
en-aut-sei=Igarashi
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=HoshinoDaichi
en-aut-sei=Hoshino
en-aut-mei=Daichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=NakajimaJunko
en-aut-sei=Nakajima
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=UedaTakayuki
en-aut-sei=Ueda
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=TaniguchiYu
en-aut-sei=Taniguchi
en-aut-mei=Yu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
en-aut-name=OgawaToru
en-aut-sei=Ogawa
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=
en-aut-name=MaekawaKenji
en-aut-sei=Maekawa
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=
en-aut-name=TamakiKatsushi
en-aut-sei=Tamaki
en-aut-mei=Katsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=
en-aut-name=KitamuraAkihiko
en-aut-sei=Kitamura
en-aut-mei=Akihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=
en-aut-name=ShinkaiShoji
en-aut-sei=Shinkai
en-aut-mei=Shoji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=
en-aut-name=HiranoHirohiko
en-aut-sei=Hirano
en-aut-mei=Hirohiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=
affil-num=1
en-affil=Department of Removable Prosthodontics and Gerodontology, Tokyo Dental College
kn-affil=
affil-num=2
en-affil=Gerodontology, Department of Oral Health Science, Faculty of Dental Medicine, Hokkaido University
kn-affil=
affil-num=3
en-affil=The Tokyo Metropolitan Support Center for Preventative Long-term and Frail Elderly Care
kn-affil=
affil-num=4
en-affil=Research Team for Promoting Independence and Mental Health, Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=5
en-affil=Research Team for Promoting Independence and Mental Health, Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=6
en-affil=Research Team for Promoting Independence and Mental Health, Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=7
en-affil=Research Team for Promoting Independence and Mental Health, Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=8
en-affil=Department of Removable Prosthodontics and Gerodontology, Tokyo Dental College
kn-affil=
affil-num=9
en-affil=Removable Prosthodontics, Nihon University School of Dentistry at Matsudo
kn-affil=
affil-num=10
en-affil=Special Needs Dentistry, Division of Community Based Comprehensive Dentistry, School of Dentistry, Showa University
kn-affil=
affil-num=11
en-affil=Department of Oral Medicine and Hospital Dentistry, Tokyo Dental College
kn-affil=
affil-num=12
en-affil=Department of Removable Prosthodontics and Gerodontology, Tokyo Dental College
kn-affil=
affil-num=13
en-affil=Center for Health and Environmental Risk Research, National Institute for Environmental Studies
kn-affil=
affil-num=14
en-affil=Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry
kn-affil=
affil-num=15
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=16
en-affil=Department of Critical Care Medicine and Dentistry, Division of Prosthodontic Dentistry for Function of TMJ and Occlusion, Graduate School of Dentistry, Kanagawa Dental University
kn-affil=
affil-num=17
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=18
en-affil=Research Team for Social Participation and Health Promotion, Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=19
en-affil=Social Sciences and Human Care, Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=20
en-affil=Research Team for Promoting Independence and Mental Health, Tokyo Metropolitan Institute of Gerontology
kn-affil=
en-keyword=Aged
kn-keyword=Aged
en-keyword=Chewing gum
kn-keyword=Chewing gum
en-keyword=Colorimetry
kn-keyword=Colorimetry
en-keyword=Color
kn-keyword=Color
en-keyword=Mastication
kn-keyword=Mastication
END
start-ver=1.4
cd-journal=joma
no-vol=65
cd-vols=
no-issue=1
article-no=
start-page=125
end-page=129
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210224
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Newly developed data-matching methodology for oral implant surgery allowing the automatic deletion of metal artifacts in 3D-CT images using new reference markers: A case report
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Patients: The patient was a 55-year-old woman with left upper molar free-end edentulism and 9 full cast metal crowns in her mouth. Three three-dimensional (3D) images were superimposed: a computed tomography (CT) image with the patient wearing the CT-matching template (CTMT) with six glass ceramic markers, which hardly generate any artifacts, on the template surface, and oral plaster model surfaces with and without CTMTs. Metal artifacts were automatically removed by a Boolean operation identifying unrealistic images outside the oral plaster model surface. After the preoperative simulation, fully guided oral implant surgery was performed. Two implant bodies were placed in the left upper edentulism. The placement errors calculated by comparing the preoperative simulation and actual implant placement were then assessed by a software program using the 3D-CT bone morphology as a reference. The 3D deviations between the preoperative simulation and actual placement at the entry of the implant body were a maximum 0.48 mm and minimum 0.26 mm. Those at the tip of the implant body were a maximum 0.56 mm and a minimum 0.25 mm.
Discussion: In this case, the maximum 3D deviations at the entry and tip section were less than in previous studies using double CT.
Conclusions: Accurate image fusion utilizing CTMT with new reference markers was possible for a patient with many metal restorations. Using a surgical guide manufactured by the new matching methodology (modified single CT scan method), implant placement deviation can be minimized in patients with many metal restorations.
en-copyright=
kn-copyright=
en-aut-name=ShimizuHiroaki
en-aut-sei=Shimizu
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=ArakawaHikaru
en-aut-sei=Arakawa
en-aut-mei=Hikaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MinoTakuya
en-aut-sei=Mino
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=KurosakiYoko
en-aut-sei=Kurosaki
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=TokumotoKana
en-aut-sei=Tokumoto
en-aut-mei=Kana
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
affil-num=1
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Computer-assisted surgery
kn-keyword=Computer-assisted surgery
en-keyword=Computer-aided design
kn-keyword=Computer-aided design
en-keyword=Tomography
kn-keyword=Tomography
en-keyword=X-Ray Computed
kn-keyword=X-Ray Computed
en-keyword=Artifact
kn-keyword=Artifact
END
start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=23
article-no=
start-page=12823
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20211126
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Suppression of Bone Necrosis around Tooth Extraction Socket in a MRONJ-like Mouse Model by E-rhBMP-2 Containing Artificial Bone Graft Administration
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Medication-related osteonecrosis of the jaw (MRONJ) is related to impaired bone healing conditions in the maxillomandibular bone region as a complication of bisphosphonate intake. Although there are several hypotheses for the onset of MRONJ symptoms, one of the possible causes is the inhibition of bone turnover and blood supply leading to bone necrosis. The optimal treatment strategy for MRONJ has not been established either. BMP-2, a member of the TGF-beta superfamily, is well known for regulating bone remodeling and homeostasis prenatally and postnatally. Therefore, the objectives of this study were to evaluate whether cyclophosphamide/zoledronate (CY/ZA) induces necrosis of the bone surrounding the tooth extraction socket, and to examine the therapeutic potential of BMP-2 in combination with the hard osteoinductive biomaterial, beta-tricalcium phosphate (beta-TCP), in the prevention and treatment of alveolar bone loss around the tooth extraction socket in MRONJ-like mice models. First, CY/ZA was intraperitoneally administered for three weeks, and alveolar bone necrosis was evaluated before and after tooth extraction. Next, the effect of BMP-2/beta-TCP was investigated in both MRONJ-like prevention and treatment models. In the prevention model, CY/ZA was continuously administered for four weeks after BMP-2/beta-TCP transplantation. In the treatment model, CY/ZA administration was suspended after transplantation of BMP-2/beta-TCP. The results showed that CY/ZA induced a significant decrease in the number of empty lacunae, a sign of bone necrosis, in the alveolar bone around the tooth extraction socket after tooth extraction. Histological analysis showed a significant decrease in the necrotic alveolar bone around tooth extraction sockets in the BMP-2/beta-TCP transplantation group compared to the non-transplanted control group in both MRONJ-like prevention and treatment models. However, bone mineral density, determined by micro-CT analysis, was significantly higher in the BMP-2/beta-TCP transplanted group than in the control group in the prevention model only. These results clarified that alveolar bone necrosis around tooth extraction sockets can be induced after surgical intervention under CY/ZA administration. In addition, transplantation of BMP-2/beta-TCP reduced the necrotic alveolar bone around the tooth extraction socket. Therefore, a combination of BMP-2/beta-TCP could be an alternative approach for both prevention and treatment of MRONJ-like symptoms.
en-copyright=
kn-copyright=
en-aut-name=TanakaYukie
en-aut-sei=Tanaka
en-aut-mei=Yukie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=AungKyaw Thu
en-aut-sei=Aung
en-aut-mei=Kyaw Thu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=OnoMitsuaki
en-aut-sei=Ono
en-aut-mei=Mitsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MikaiAkihiro
en-aut-sei=Mikai
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=DangAnh Tuan
en-aut-sei=Dang
en-aut-mei=Anh Tuan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=HaraEmilio Satoshi
en-aut-sei=Hara
en-aut-mei=Emilio Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=TosaIkue
en-aut-sei=Tosa
en-aut-mei=Ikue
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=IshibashiKei
en-aut-sei=Ishibashi
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=Ono-KimuraAya
en-aut-sei=Ono-Kimura
en-aut-mei=Aya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=NawachiKumiko
en-aut-sei=Nawachi
en-aut-mei=Kumiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=OohashiToshitaka
en-aut-sei=Oohashi
en-aut-mei=Toshitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
affil-num=1
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Oral Rehabilitation and Implantology, Okayama University Hospital
kn-affil=
affil-num=5
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=7
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=8
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=9
en-affil=Department of Oral Rehabilitation and Implantology, Okayama University Hospital
kn-affil=
affil-num=10
en-affil=Department of Oral Rehabilitation and Implantology, Okayama University Hospital
kn-affil=
affil-num=11
en-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=12
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=medication-related osteonecrosis of the jaw
kn-keyword=medication-related osteonecrosis of the jaw
en-keyword=BMP-2
kn-keyword=BMP-2
en-keyword=beta-tricalcium phosphate
kn-keyword=beta-tricalcium phosphate
en-keyword=bone formation
kn-keyword=bone formation
en-keyword=bone necrosis
kn-keyword=bone necrosis
END
start-ver=1.4
cd-journal=joma
no-vol=28
cd-vols=
no-issue=1
article-no=
start-page=37
end-page=42
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Achievements and Next Themes of Dental Section of Perioperative Management Center in Okayama University Hospital at 5 Months after Establishment
kn-title=岡山大学病院周術期管理センター(歯科部門)設立後5ヵ月間の活動内容および今後の展開
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=YamanakaReiko
en-aut-sei=Yamanaka
en-aut-mei=Reiko
kn-aut-name=山中玲子
kn-aut-sei=山中
kn-aut-mei=玲子
aut-affil-num=1
ORCID=
en-aut-name=SogaYoshihiko
en-aut-sei=Soga
en-aut-mei=Yoshihiko
kn-aut-name=曽我賢彦
kn-aut-sei=曽我
kn-aut-mei=賢彦
aut-affil-num=2
ORCID=
en-aut-name=NawachiKumiko
en-aut-sei=Nawachi
en-aut-mei=Kumiko
kn-aut-name=縄稚久美子
kn-aut-sei=縄稚
kn-aut-mei=久美子
aut-affil-num=3
ORCID=
en-aut-name=YanagiYoshinobu
en-aut-sei=Yanagi
en-aut-mei=Yoshinobu
kn-aut-name=柳文修
kn-aut-sei=柳
kn-aut-mei=文修
aut-affil-num=4
ORCID=
en-aut-name=KodamaNaoki
en-aut-sei=Kodama
en-aut-mei=Naoki
kn-aut-name=兒玉直紀
kn-aut-sei=兒玉
kn-aut-mei=直紀
aut-affil-num=5
ORCID=
en-aut-name=NakataTakashi
en-aut-sei=Nakata
en-aut-mei=Takashi
kn-aut-name=中田貴
kn-aut-sei=中田
kn-aut-mei=貴
aut-affil-num=6
ORCID=
en-aut-name=MiuraRumi
en-aut-sei=Miura
en-aut-mei=Rumi
kn-aut-name=三浦留美
kn-aut-sei=三浦
kn-aut-mei=留美
aut-affil-num=7
ORCID=
en-aut-name=HagawaMisao
en-aut-sei=Hagawa
en-aut-mei=Misao
kn-aut-name=羽川操
kn-aut-sei=羽川
kn-aut-mei=操
aut-affil-num=8
ORCID=
en-aut-name=TakeuchiTetsuo
en-aut-sei=Takeuchi
en-aut-mei=Tetsuo
kn-aut-name=竹内哲男
kn-aut-sei=竹内
kn-aut-mei=哲男
aut-affil-num=9
ORCID=
en-aut-name=YamaneMieko
en-aut-sei=Yamane
en-aut-mei=Mieko
kn-aut-name=山根美榮子
kn-aut-sei=山根
kn-aut-mei=美榮子
aut-affil-num=10
ORCID=
en-aut-name=MoritaManabu
en-aut-sei=Morita
en-aut-mei=Manabu
kn-aut-name=森田学
kn-aut-sei=森田
kn-aut-mei=学
aut-affil-num=11
ORCID=
en-aut-name=TakashibaShogo
en-aut-sei=Takashiba
en-aut-mei=Shogo
kn-aut-name=高柴正悟
kn-aut-sei=高柴
kn-aut-mei=正悟
aut-affil-num=12
ORCID=
en-aut-name=AsamiJun-ichi
en-aut-sei=Asami
en-aut-mei=Jun-ichi
kn-aut-name=浅海淳一
kn-aut-sei=浅海
kn-aut-mei=淳一
aut-affil-num=13
ORCID=
en-aut-name=MinagiShogo
en-aut-sei=Minagi
en-aut-mei=Shogo
kn-aut-name=皆木省吾
kn-aut-sei=皆木
kn-aut-mei=省吾
aut-affil-num=14
ORCID=
en-aut-name=YoshiyamaMasahiro
en-aut-sei=Yoshiyama
en-aut-mei=Masahiro
kn-aut-name=吉山昌宏
kn-aut-sei=吉山
kn-aut-mei=昌宏
aut-affil-num=15
ORCID=
en-aut-name=ShimonoTsutomu
en-aut-sei=Shimono
en-aut-mei=Tsutomu
kn-aut-name=下野勉
kn-aut-sei=下野
kn-aut-mei=勉
aut-affil-num=16
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=窪木拓男
kn-aut-sei=窪木
kn-aut-mei=拓男
aut-affil-num=17
ORCID=
en-aut-name=SasakiAkira
en-aut-sei=Sasaki
en-aut-mei=Akira
kn-aut-name=佐々木朗
kn-aut-sei=佐々木
kn-aut-mei=朗
aut-affil-num=18
ORCID=
en-aut-name=MoritaKiyoshi
en-aut-sei=Morita
en-aut-mei=Kiyoshi
kn-aut-name=森田潔
kn-aut-sei=森田
kn-aut-mei=潔
aut-affil-num=19
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学病院周術期管理センター(歯科部門)
affil-num=2
en-affil=
kn-affil=岡山大学病院周術期管理センター(歯科部門)
affil-num=3
en-affil=
kn-affil=岡山大学病院周術期管理センター(歯科部門)
affil-num=4
en-affil=
kn-affil=岡山大学病院周術期管理センター(歯科部門)
affil-num=5
en-affil=
kn-affil=岡山大学病院周術期管理センター(歯科部門)
affil-num=6
en-affil=
kn-affil=岡山大学病院周術期管理センター(歯科部門)
affil-num=7
en-affil=
kn-affil=岡山大学病院周術期管理センター(歯科部門)
affil-num=8
en-affil=
kn-affil=岡山大学病院医療技術部歯科衛生士室
affil-num=9
en-affil=
kn-affil=岡山大学病院医療技術部歯科技工室
affil-num=10
en-affil=
kn-affil=岡山大学病院周術期管理センター(歯科部門)
affil-num=11
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科予防歯科学分野
affil-num=12
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科歯周病態学分野
affil-num=13
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科歯科放射線学分野
affil-num=14
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科咬合・有床義歯補綴学分野
affil-num=15
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科歯科保存修復学分野
affil-num=16
en-affil=
kn-affil=岡山大学病院医療技術部歯科衛生士室
affil-num=17
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科インプラント再生補綴学分野
affil-num=18
en-affil=
kn-affil=岡山大学病院副病院長
affil-num=19
en-affil=
kn-affil=岡山大学病院病院長
END
start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=12
article-no=
start-page=1032
end-page=1039
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20221121
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Impact of number of functional teeth on independence of Japanese older adults
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Aim
To examine the relationship between the number of present and functional teeth at baseline and future incidence of loss of independence.
Methods
Participants were community-dwelling older individuals who participated in a comprehensive geriatric health examination conducted in Kusatsu town, Japan, between 2009 and 2015. The primary endpoint was the incidence of loss of independence among participants, defined as the first certification of long-term care insurance in Japan. The numbers of present and functional teeth at baseline were determined via an oral examination. Demographics, clinical variables (e.g., history of chronic diseases and psychosocial factors), blood nutritional markers, physical functions, and perceived masticatory function were assessed.
Results
This study included 1121 individuals, and 205 individuals suffered from loss of independence during the follow-up period. Kaplan?Meier estimates of loss of independence for participants with smaller numbers of present and functional teeth were significantly greater than for those with larger numbers of teeth. Cox proportional hazard analyses indicated that a smaller number of present teeth was not a significant risk factor after adjusting for demographic characteristics. However, the number of functional teeth was a significant risk factor after the adjustment (hazard ratio: 1.975 [1.168?3.340]). Additionally, higher hazard ratios were observed in other adjusted models, but they were not statistically significant.
Conclusions
The number of functional teeth may be more closely related to the future incidence of loss of independence than the number of present teeth. This novel finding suggests that prosthodontic rehabilitation for tooth loss possibly prevents the future incidence of this life-event.
en-copyright=
kn-copyright=
en-aut-name=MaekawaKenji
en-aut-sei=Maekawa
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=IkeuchiTomoko
en-aut-sei=Ikeuchi
en-aut-mei=Tomoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ShinkaiShoji
en-aut-sei=Shinkai
en-aut-mei=Shoji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=HiranoHirohiko
en-aut-sei=Hirano
en-aut-mei=Hirohiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=RyuMasahiro
en-aut-sei=Ryu
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=TamakiKatsushi
en-aut-sei=Tamaki
en-aut-mei=Katsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=YataniHirofumi
en-aut-sei=Yatani
en-aut-mei=Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=Kimura‐OnoAya
en-aut-sei=Kimura‐Ono
en-aut-mei=Aya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=KikutaniTakeshi
en-aut-sei=Kikutani
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=SuganumaTakashi
en-aut-sei=Suganuma
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=AyukawaYasunori
en-aut-sei=Ayukawa
en-aut-mei=Yasunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=GondaTomoya
en-aut-sei=Gonda
en-aut-mei=Tomoya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
en-aut-name=OgawaToru
en-aut-sei=Ogawa
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=
en-aut-name=FujisawaMasanori
en-aut-sei=Fujisawa
en-aut-mei=Masanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=
en-aut-name=IshigakiShoichi
en-aut-sei=Ishigaki
en-aut-mei=Shoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=
en-aut-name=WatanabeYutaka
en-aut-sei=Watanabe
en-aut-mei=Yutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=
en-aut-name=KitamuraAkihiko
en-aut-sei=Kitamura
en-aut-mei=Akihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=
en-aut-name=TaniguchiYu
en-aut-sei=Taniguchi
en-aut-mei=Yu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=
en-aut-name=FujiwaraYoshinori
en-aut-sei=Fujiwara
en-aut-mei=Yoshinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=
en-aut-name=EdahiroAyako
en-aut-sei=Edahiro
en-aut-mei=Ayako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=
en-aut-name=OharaYuki
en-aut-sei=Ohara
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=
en-aut-name=FuruyaJunichi
en-aut-sei=Furuya
en-aut-mei=Junichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=23
ORCID=
en-aut-name=NakajimaJunko
en-aut-sei=Nakajima
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=24
ORCID=
en-aut-name=UmekiKento
en-aut-sei=Umeki
en-aut-mei=Kento
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=25
ORCID=
en-aut-name=IgarashiKentaro
en-aut-sei=Igarashi
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=26
ORCID=
en-aut-name=HoribeYasuhiro
en-aut-sei=Horibe
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=27
ORCID=
en-aut-name=KugimiyaYoshihiro
en-aut-sei=Kugimiya
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=28
ORCID=
en-aut-name=KawaiYasuhiko
en-aut-sei=Kawai
en-aut-mei=Yasuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=29
ORCID=
en-aut-name=MatsumuraHideo
en-aut-sei=Matsumura
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=30
ORCID=
en-aut-name=IchikawaTetsuo
en-aut-sei=Ichikawa
en-aut-mei=Tetsuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=31
ORCID=
en-aut-name=OhkawaShuji
en-aut-sei=Ohkawa
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=32
ORCID=
en-aut-name=BabaKazuyoshi
en-aut-sei=Baba
en-aut-mei=Kazuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=33
ORCID=
en-aut-name=Kusatsu ISLE Study Working Group Collaborators
en-aut-sei=Kusatsu ISLE Study Working Group Collaborators
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=34
ORCID=
affil-num=1
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=3
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=4
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=5
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=6
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=7
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=8
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=9
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=10
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=11
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=12
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=13
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=14
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=15
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=16
en-affil=Research Planning and Promotion Committee Japan Prosthodontic Society
kn-affil=
affil-num=17
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=18
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=19
en-affil=National Institute for Environmental Studies
kn-affil=
affil-num=20
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=21
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=22
en-affil=Tokyo Metropolitan Institute of Gerontology
kn-affil=
affil-num=23
en-affil=Showa University School of Dentistry
kn-affil=
affil-num=24
en-affil=Tokyo Dental College
kn-affil=
affil-num=25
en-affil=Nihon University School of Dentistry at Matsudo
kn-affil=
affil-num=26
en-affil=Nihon University School of Dentistry at Matsudo
kn-affil=
affil-num=27
en-affil=Tokyo Dental College
kn-affil=
affil-num=28
en-affil=Tokyo Dental College
kn-affil=
affil-num=29
en-affil=Nihon University School of Dentistry at Matsudo
kn-affil=
affil-num=30
en-affil=Nihon University School of Dentistry
kn-affil=
affil-num=31
en-affil=Tokushima University Graduate School Institute of Biomedical Sciences
kn-affil=
affil-num=32
en-affil=Meikai University School of Dentistry
kn-affil=
affil-num=33
en-affil=Showa University School of Dentistry
kn-affil=
affil-num=34
en-affil=
kn-affil=
en-keyword=community-dwelling older adults
kn-keyword=community-dwelling older adults
en-keyword=functional teeth
kn-keyword=functional teeth
en-keyword=loss of independence
kn-keyword=loss of independence
en-keyword=oral health
kn-keyword=oral health
en-keyword=present teeth
kn-keyword=present teeth
END
start-ver=1.4
cd-journal=joma
no-vol=62
cd-vols=
no-issue=1
article-no=
start-page=59
end-page=62
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Dental Implant Treatment for a Patient with Bilateral Cleft Lip and Palate
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
Dental reconstruction in the cleft space is difficult in some patients with cleft lip and palate because of oronasal fistulas. Most of these patients receive a particle cancellous bone marrow (PCBM) graft to close the alveolar cleft, and secondary bone grafting is also required. Treatment options for the alveolar cleft including fixed or removable prostheses require the preparation of healthy teeth and are associated with functional or social difficulties. Recently, the effectiveness of dental implant treatment for cleft lip and palate patients has been reported. However, there have been few reports on the use of this treatment in bilateral cleft lip and palate patients. We report the case of a patient who had bilateral cleft lip and palate and was missing both lateral incisors. She received dental implant treatment after a PCBM graft and ramus bone onlay grafting (RBOG). A 34-month postoperative course was uneventful.
en-copyright= kn-copyright= en-aut-name=SawakiMasako en-aut-sei=Sawaki en-aut-mei=Masako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UenoTakaaki en-aut-sei=Ueno en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KagawaToshimasa en-aut-sei=Kagawa en-aut-mei=Toshimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KanouMiwa en-aut-sei=Kanou en-aut-mei=Miwa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HondaKozo en-aut-sei=Honda en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShirasuNobuaki en-aut-sei=Shirasu en-aut-mei=Nobuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KubokiTakuo en-aut-sei=Kuboki en-aut-mei=Takuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SugaharaToshio en-aut-sei=Sugahara en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=dental implant kn-keyword=dental implant en-keyword=cleft lip and palate kn-keyword=cleft lip and palate en-keyword=dental reconstruction kn-keyword=dental reconstruction END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140813 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy en-subtitle= kn-subtitle= en-abstract= kn-abstract=Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy. en-copyright= kn-copyright= en-aut-name=OshimaMasamitsu en-aut-sei=Oshima en-aut-mei=Masamitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InoueKaoru en-aut-sei=Inoue en-aut-mei=Kaoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakajimaKei en-aut-sei=Nakajima en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TachikawaTetsuhiko en-aut-sei=Tachikawa en-aut-mei=Tetsuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YamazakiHiromichi en-aut-sei=Yamazaki en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IsobeTomohide en-aut-sei=Isobe en-aut-mei=Tomohide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SugawaraAyaka en-aut-sei=Sugawara en-aut-mei=Ayaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OgawaMiho en-aut-sei=Ogawa en-aut-mei=Miho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TanakaChie en-aut-sei=Tanaka en-aut-mei=Chie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=SaitoMasahiro en-aut-sei=Saito en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KasugaiShohei en-aut-sei=Kasugai en-aut-mei=Shohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=Takano-YamamotoTeruko en-aut-sei=Takano-Yamamoto en-aut-mei=Teruko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=InoueTakashi en-aut-sei=Inoue en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=TezukaKatsunari en-aut-sei=Tezuka en-aut-mei=Katsunari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=KubokiTakuo en-aut-sei=Kuboki en-aut-mei=Takuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=YamaguchiAkira en-aut-sei=Yamaguchi en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=TsujiTakashi en-aut-sei=Tsuji en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= affil-num=1 en-affil= kn-affil=Tokyo Univ Sci, Res Inst Sci & Technol affil-num=2 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=3 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=4 en-affil= kn-affil=Showa Univ, Sch Dent, Dept Oral Pathol affil-num=5 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=6 en-affil= kn-affil=Showa Univ, Sch Dent, Dept Oral Pathol affil-num=7 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=8 en-affil= kn-affil=Tokyo Univ Sci, Res Inst Sci & Technol affil-num=9 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=10 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=11 en-affil= kn-affil=Tokyo Med & Dent Univ, Grad Sch, Sect Oral Implantol & Regenerat Dent Med affil-num=12 en-affil= kn-affil=Tohoku Univ, Grad Sch Dent, Div Orthodont & Dentofacial Orthoped affil-num=13 en-affil= kn-affil=Tokyo Dent Coll, Dept Clin Pathophysiol affil-num=14 en-affil= kn-affil=Tokyo Univ Sci, Res Inst Sci & Technol affil-num=15 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Oral Rehabil & Regenerat Med affil-num=16 en-affil= kn-affil=Tokyo Med & Dent Univ, Grad Sch, Dept Oral Restitut, Sect Oral Pathol affil-num=17 en-affil= kn-affil=Tokyo Univ Sci, Res Inst Sci & Technol END start-ver=1.4 cd-journal=joma no-vol=11 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=201505 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Compound-protein combination shows promise for arthritis treatment en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KubokiTakuo en-aut-sei=Kuboki en-aut-mei=Takuo kn-aut-name=窪木拓男 kn-aut-sei=窪木 kn-aut-mei=拓男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Department of Oral Rehabilitation and Regenerative Medicine Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=11468 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=2015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro en-subtitle= kn-subtitle= en-abstract= kn-abstract=In vitro fabricated biological tissue would be a valuable tool to screen newly synthesized drugs or understand the tissue development process. Several studies have attempted to fabricate biological tissue in vitro. However, controlling the growth and morphology of the fabricated tissue remains a challenge. Therefore, new techniques are required to modulate tissue growth. RGD (arginine-glycine-aspartic acid), which is an integrin-binding domain of fibronectin, has been found to enhance cell adhesion and survival; it has been used to modify substrates for in vitro cell culture studies or used as tissue engineering scaffolds. In addition, this study shows novel functions of the RGD peptide, which enhances tissue growth and modulates tissue morphology in vitro. When an isolated submandibular gland (SMG) was cultured on an RGD-modified alginate hydrogel sheet, SMG growth including bud expansion and cleft formation was dramatically enhanced. Furthermore, we prepared small RGD-modified alginate beads and placed them on the growing SMG tissue. These RGD-modified beads successfully induced cleft formation at the bead position, guiding the desired SMG morphology. Thus, this RGD-modified material might be a promising tool to modulate tissue growth and morphology in vitro for biological tissue fabrication. en-copyright= kn-copyright= en-aut-name=TaketaHiroaki en-aut-sei=Taketa en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=GulsanAra Sathi en-aut-sei=Gulsan en-aut-mei=Ara Sathi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MahmoudFarahat en-aut-sei=Mahmoud en-aut-mei=Farahat kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KaziAnisur Rahman en-aut-sei=Kazi en-aut-mei=Anisur Rahman kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SakaiTakayoshi en-aut-sei=Sakai en-aut-mei=Takayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HiranoYoshiaki en-aut-sei=Hirano en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KubokiTakuo en-aut-sei=Kuboki en-aut-mei=Takuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ToriiYasuhiro en-aut-sei=Torii en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MatsumotoTakuya en-aut-sei=Matsumoto en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=2 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=3 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=4 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=5 en-affil= kn-affil=Department of Oral-Facial Disorders, Osaka University affil-num=6 en-affil= kn-affil=Department of Chemical Engineering, Kansai University affil-num=7 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=8 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=9 en-affil= kn-affil=Department of Biomaterials, Okayama University END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=12 article-no= start-page=e83545 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20131227 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=miRNA-720 Controls Stem Cell Phenotype, Proliferation and Differentiation of Human Dental Pulp Cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=Dental pulp cells (DPCs) are known to be enriched in stem/progenitor cells but not well characterized yet. Small non-coding microRNAs (miRNAs) have been identified to control protein translation, mRNA stability and transcription, and have been reported to play important roles in stem cell biology, related to cell reprogramming, maintenance of stemness and regulation of cell differentiation. In order to characterize dental pulp stem/progenitor cells and its mechanism of differentiation, we herein sorted stem-cell-enriched side population (SP) cells from human DPCs and periodontal ligament cells (PDLCs), and performed a locked nucleic acid (LNA)-based miRNA array. As a result, miR-720 was highly expressed in the differentiated main population (MP) cells compared to that in SP cells. In silico analysis and a reporter assay showed that miR-720 targets the stem cell marker NANOG, indicating that miR-720 could promote differentiation of dental pulp stem/progenitor cells by repressing NANOG. Indeed, gain-and loss-of-function analyses showed that miR-720 controls NANOG transcript and protein levels. Moreover, transfection of miR-720 significantly decreased the number of cells positive for the early stem cell marker SSEA-4. Concomitantly, mRNA levels of DNA methyltransferases (DNMTs), which are known to play crucial factors during stem cell differentiation, were also increased by miR-720 through unknown mechanism. Finally, miR-720 decreased DPC proliferation as determined by immunocytochemical analysis against ki-67, and promoted odontogenic differentiation as demonstrated by alizarin red staining, as well as alkaline phosphatase and osteopontin mRNA levels. Our findings identify miR-720 as a novel miRNA regulating the differentiation of DPCs. en-copyright= kn-copyright= en-aut-name=HaraEmilio Satoshi en-aut-sei=Hara en-aut-mei=Emilio Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OnoMitsuaki en-aut-sei=Ono en-aut-mei=Mitsuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=EguchiTakanori en-aut-sei=Eguchi en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KubotaSatoshi en-aut-sei=Kubota en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HaiThanh Pham en-aut-sei=Hai en-aut-mei=Thanh Pham kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SonoyamaWataru en-aut-sei=Sonoyama en-aut-mei=Wataru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TajimaShoji en-aut-sei=Tajima en-aut-mei=Shoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TakigawaMasaharu en-aut-sei=Takigawa en-aut-mei=Masaharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=Stuart K.Calderwood en-aut-sei=Stuart K. en-aut-mei=Calderwood kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KubokiTakuo en-aut-sei=Kuboki en-aut-mei=Takuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Radiation Oncology, Division of Molecular and Cellular Biology, Beth Israel Deaconess Medical Center, Harvard Medical School affil-num=4 en-affil= kn-affil=Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Laboratory of Epigenetics, Institute for Protein Research, Osaka University affil-num=8 en-affil= kn-affil=Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Department of Radiation Oncology, Division of Molecular and Cellular Biology, Beth Israel Deaconess Medical Center, Harvard Medical School affil-num=10 en-affil= kn-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=3 article-no= start-page=e58796 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130322 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=OstemiR: A Novel Panel of MicroRNA Biomarkers in Osteoblastic and Osteocytic Differentiation from Mesencymal Stem Cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= MicroRNAs (miRNAs) are small RNA molecules of 21?25 nucleotides that regulate cell behavior through inhibition of translation from mRNA to protein, promotion of mRNA degradation and control of gene transcription. In this study, we investigated the miRNA expression signatures of cell cultures undergoing osteoblastic and osteocytic differentiation from mesenchymal stem cells (MSC) using mouse MSC line KUSA-A1 and human MSCs. Ninety types of miRNA were quantified during osteoblastic/osteocytic differentiation in KUSA-A1 cells utilizing miRNA PCR arrays. Coincidently with mRNA induction of the osteoblastic and osteocytic markers, the expression levels of several dozen miRNAs including miR-30 family, let-7 family, miR-21, miR-16, miR-155, miR-322 and Snord85 were changed during the differentiation process. These miRNAs were predicted to recognize osteogenic differentiation-, stemness-, epinegetics-, and cell cycle-related mRNAs, and were thus designated OstemiR. Among those OstemiR, the miR-30 family was classified into miR-30b/c and miR-30a/d/e groups on the basis of expression patterns during osteogenesis as well as mature miRNA structures. In silico prediction and subsequent qRT-PCR in stable miR-30d transfectants clarified that context-dependent targeting of miR-30d on known regulators of bone formation including osteopontin/spp1, lifr, ccn2/ctgf, ccn1/cyr61, runx2, sox9 as well as novel key factors including lin28a, hnrnpa3, hspa5/grp78, eed and pcgf5. In addition, knockdown of human OstemiR miR-541 increased Osteopontin/SPP1 expression and calcification in hMSC osteoblastic differentiation, indicating that miR-541 is a negative regulator of osteoblastic differentiation. These observations indicate stage-specific roles of OstemiR especially miR-541 and the miR-30 family on novel targets in osteogenesis. en-copyright= kn-copyright= en-aut-name=EguchiTakanori en-aut-sei=Eguchi en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeKen en-aut-sei=Watanabe en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HaraEmilio Satoshi en-aut-sei=Hara en-aut-mei=Emilio Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OnoMitsuaki en-aut-sei=Ono en-aut-mei=Mitsuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KubokiTakuo en-aut-sei=Kuboki en-aut-mei=Takuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=Stuart K.Calderwood en-aut-sei=Stuart K. en-aut-mei=Calderwood kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Department of Oral Disease Research, National Center for Geriatrics and Gerontology affil-num=2 en-affil= kn-affil=Department of Bone and Joint Disease, National Center for Geriatrics and Gerontology affil-num=3 en-affil= kn-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Division of Molecular and Cellular Biology, Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School END start-ver=1.4 cd-journal=joma no-vol=121 cd-vols= no-issue=3 article-no= start-page=183 end-page=187 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20091201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Two programs supporting community dentistry: an oral implant course and education about dysphagia rehabilitation kn-title=地域の歯科医療関係者に対する支援―口腔インプラント講習会と摂食・ 嚥下リハビリテーション教育― en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=AzumaTetsuji en-aut-sei=Azuma en-aut-mei=Tetsuji kn-aut-name=東哲司 kn-aut-sei=東 kn-aut-mei=哲司 aut-affil-num=1 ORCID= en-aut-name=AriokaKyoko en-aut-sei=Arioka en-aut-mei=Kyoko kn-aut-name=有岡享子 kn-aut-sei=有岡 kn-aut-mei=享子 aut-affil-num=2 ORCID= en-aut-name=ArakawaHikaru en-aut-sei=Arakawa en-aut-mei=Hikaru kn-aut-name=荒川光 kn-aut-sei=荒川 kn-aut-mei=光 aut-affil-num=3 ORCID= en-aut-name=EgusaMasahiko en-aut-sei=Egusa en-aut-mei=Masahiko kn-aut-name=江草正彦 kn-aut-sei=江草 kn-aut-mei=正彦 aut-affil-num=4 ORCID= en-aut-name=KubokiTakuo en-aut-sei=Kuboki en-aut-mei=Takuo kn-aut-name=窪木拓男 kn-aut-sei=窪木 kn-aut-mei=拓男 aut-affil-num=5 ORCID= en-aut-name=MoritaManabu en-aut-sei=Morita en-aut-mei=Manabu kn-aut-name=森田学 kn-aut-sei=森田 kn-aut-mei=学 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 予防歯科学 affil-num=2 en-affil= kn-affil=岡山大学病院 特殊歯科総合治療部第1総合診療室 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 インプラント再生補綴学 affil-num=4 en-affil= kn-affil=岡山大学病院 特殊歯科総合治療部第1総合診療室 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 インプラント再生補綴学 affil-num=6 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 予防歯科学 en-keyword=地域支援 kn-keyword=地域支援 en-keyword=口腔インプラント kn-keyword=口腔インプラント en-keyword=摂食 kn-keyword=摂食 en-keyword=嚥下リハビリテーション kn-keyword=嚥下リハビリテーション END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=1 article-no= start-page=42 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20231102 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Accuracy of a novel modified single computed tomography scanning method for assisting dental implant placement: a retrospective observational study en-subtitle= kn-subtitle= en-abstract= kn-abstract=Purpose The aim of this study is to compare dental implant placement accuracy of three surgical guide fabrication methods: single (SCT) and double computed tomography (DCT), and a newly developed modified SCT (MSCT) scan method.