start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue= article-no= start-page=647684 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210810 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification, Characterization, and Gene Expression of Rice Endo-beta-N-Acetylglucosaminidase, Endo-Os en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the endoplasmic reticulum-associated degradation system of plant and animal cells, high-mannose type free N-glycans (HMT-FNGs) are produced from misfolded glycoproteins prior to proteasomal degradation, and two enzymes, cytosolic peptide:N-glycanase (cPNGase) and endo-beta-N-acetylglucosaminidase (endo-beta-GlcNAc-ase), are involved in the deglycosylation. Although the physiological functions of these FNGs in plant growth and development remain to be elucidated, detailed characterization of cPNGase and endo-beta-GlcNAc-ase is required. In our previous work, we described the purification, characterization, and subcellular distribution of some plant endo-beta-GlcNAc-ases and preliminarily reported the gene information of rice endo-beta-GlcNAc-ase (Endo-Os). Furthermore, we analyzed the changes in gene expression of endo-beta-GlcNAc-ase during tomato fruit maturation and constructed a mutant line of Arabidopsis thaliana, in which the two endo-beta-GlcNAc-ase genes were knocked-out based on the Endo-Os gene. In this report, we describe the purification, characterization, amino acid sequence, and gene cloning of Endo-Os in detail. Purified Endo-Os, with an optimal pH of 6.5, showed high activity for high-mannose type N-glycans bearing the Man alpha 1-2Man alpha 1-3Man beta 1 unit; this substrate specificity was almost the same as that of other plant endo-beta-GlcNAc-ases, suggesting that Endo-Os plays a critical role in the production of HTM-FNGs in the cytosol. Electrospray ionization-mass spectrometry analysis of the tryptic peptides revealed 17 internal amino acid sequences, including the C terminus; the N-terminal sequence could not be identified due to chemical modification. These internal amino acid sequences were consistent with the amino acid sequence (UniProt ID: Q5W6R1) deduced from the Oryza sativa cDNA clone AK112067 (gene ID: Os05g0346500). Recombinant Endo-Os expressed in Escherichia coli using cDNA showed the same enzymatic properties as those of native Endo-Os. en-copyright= kn-copyright= en-aut-name=MaedaMegumi en-aut-sei=Maeda en-aut-mei=Megumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkamotoNaoko en-aut-sei=Okamoto en-aut-mei=Naoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ArakiNode en-aut-sei=Araki en-aut-mei=Node kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University kn-affil= affil-num=4 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=endo-beta-N-acetylglucosaminidase kn-keyword=endo-beta-N-acetylglucosaminidase en-keyword=free N-glycans kn-keyword=free N-glycans en-keyword=Oryza sativa kn-keyword=Oryza sativa en-keyword=ER associated degradation kn-keyword=ER associated degradation en-keyword=peptide:N-glycanase kn-keyword=peptide:N-glycanase END start-ver=1.4 cd-journal=joma no-vol=11 cd-vols= no-issue= article-no= start-page=610124 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210118 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cytosolic Free N-Glycans Are Retro-Transported Into the Endoplasmic Reticulum in Plant Cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=During endoplasmic reticulum (ER)-associated degradation, free N-glycans (FNGs) are produced from misfolded nascent glycoproteins via the combination of the cytosolic peptide N-glycanase (cPNGase) and endo-beta-N-acetylglucosaminidase (ENGase) in the plant cytosol. The resulting high-mannose type (HMT)-FNGs, which carry one GlcNAc residue at the reducing end (GN1-FNGs), are ubiquitously found in developing plant cells. In a previous study, we found that HMT-FNGs assisted in protein folding and inhibited beta-amyloid fibril formation, suggesting a possible biofunction of FNGs involved in the protein folding system. However, whether these HMT-FNGs occur in the ER, an organelle involved in protein folding, remained unclear. On the contrary, we also reported the presence of plant complex type (PCT)-GN1-FNGs, which carry the Lewis(a) epitope at the non-reducing end, indicating that these FNGs had been fully processed in the Golgi apparatus. Since plant ENGase was active toward HMT-N-glycans but not PCT-N-glycans that carry beta 1-2xylosyl and/or alpha 1-3 fucosyl residue(s), these PCT-GN1-FNGs did not appear to be produced from fully processed glycoproteins that harbored PCT-N-glycans via ENGase activity. Interestingly, PCT-GN1-FNGs were found in the extracellular space, suggesting that HMT-GN1-FNGs formed in the cytosol might be transported back to the ER and processed in the Golgi apparatus through the protein secretion pathway. As the first step in elucidating the production mechanism of PCT-GN1-FNGs, we analyzed the structures of free oligosaccharides in plant microsomes and proved that HMT-FNGs (Man(9-7)GlcNAc(1) and Man(9-8)GlcNAc(2)) could be found in microsomes, which almost consist of the ER compartments. en-copyright= kn-copyright= en-aut-name=KatsubeMakoto en-aut-sei=Katsube en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EbaraNatsuki en-aut-sei=Ebara en-aut-mei=Natsuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MaedaMegumi en-aut-sei=Maeda en-aut-mei=Megumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=free N-glycans kn-keyword=free N-glycans en-keyword=ER-associated degradation kn-keyword=ER-associated degradation en-keyword=peptide:N-glycanase kn-keyword=peptide:N-glycanase en-keyword=endo-beta-N-acetylglucosaminidase kn-keyword=endo-beta-N-acetylglucosaminidase en-keyword=plant glycoproteins kn-keyword=plant glycoproteins END start-ver=1.4 cd-journal=joma no-vol=83 cd-vols= no-issue=7 article-no= start-page=1310 end-page=1314 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190425 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Plant complex type free N-glycans occur in tomato xylem sap en-subtitle= kn-subtitle= en-abstract= kn-abstract= Free N-glycans (FNGs) are ubiquitous in growing plants. Further, acidic peptide:N-glycanase is believed to be involved in the production of plant complex-type FNGs (PCT-FNGs) during the degradation of dysfunctional glycoproteins. However, the distribution of PCT-FNGs in growing plants has not been analyzed. Here, we report the occurrence of PCT-FNGs in the xylem sap of the stem of the tomato plant. en-copyright= kn-copyright= en-aut-name=TsujimoriYuta en-aut-sei=Tsujimori en-aut-mei=Yuta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OguraMikako en-aut-sei=Ogura en-aut-mei=Mikako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Md. Ziaur Rahman en-aut-sei=Md. Ziaur Rahman en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MaedaMegumi en-aut-sei=Maeda en-aut-mei=Megumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University kn-affil= affil-num=2 en-affil=Faculty of Agriculture, Division of Agricultural Science , Okayama University kn-affil= affil-num=3 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University kn-affil= affil-num=4 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University kn-affil= affil-num=5 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University kn-affil= en-keyword=Free -glycan kn-keyword=Free -glycan en-keyword=PNGase kn-keyword=PNGase en-keyword=deglycosylation kn-keyword=deglycosylation en-keyword=solanum lycopersicum kn-keyword=solanum lycopersicum en-keyword=xylem sap kn-keyword=xylem sap END start-ver=1.4 cd-journal=joma no-vol=82 cd-vols= no-issue=7 article-no= start-page=1172 end-page=1175 dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=201804 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Novel assay system for acidic Peptide:N-glycanase (aPNGase) activity in crude plant extract en-subtitle= kn-subtitle= en-abstract= kn-abstract= Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials en-copyright= kn-copyright= en-aut-name=UemuraRyota en-aut-sei=Uemura en-aut-mei=Ryota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OguraMikako en-aut-sei=Ogura en-aut-mei=Mikako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsumaruChihiro en-aut-sei=Matsumaru en-aut-mei=Chihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AkiyamaTsuyoshi en-aut-sei=Akiyama en-aut-mei=Tsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MaedaMegumi en-aut-sei=Maeda en-aut-mei=Megumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University kn-affil= affil-num=2 en-affil=Faculty of Agriculture , Okayama University kn-affil= affil-num=3 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University kn-affil= affil-num=4 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University kn-affil= affil-num=5 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University kn-affil= affil-num=6 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University kn-affil= en-keyword=Acidic PNGase kn-keyword=Acidic PNGase en-keyword=FNG: free N-glycan kn-keyword=FNG: free N-glycan en-keyword=Fuc: L-fucose kn-keyword=Fuc: L-fucose en-keyword=Gal: D-galactose kn-keyword=Gal: D-galactose en-keyword=GlcNAc: N-acetyl-D-glucosamine kn-keyword=GlcNAc: N-acetyl-D-glucosamine en-keyword=HPLC: high-performance liquid chromatography kn-keyword=HPLC: high-performance liquid chromatography en-keyword=Man: D-mannose kn-keyword=Man: D-mannose en-keyword=NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAc kn-keyword=NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAc en-keyword=NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAc kn-keyword=NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAc en-keyword=NeuNAc: N-acetylneuraminic acid kn-keyword=NeuNAc: N-acetylneuraminic acid en-keyword=PA-: pyridylamino kn-keyword=PA-: pyridylamino en-keyword=PNGase-A: aPNGase from almond seed kn-keyword=PNGase-A: aPNGase from almond seed en-keyword=PNGase-Le: aPNGase from tomato (Solanum lycopersium L.) kn-keyword=PNGase-Le: aPNGase from tomato (Solanum lycopersium L.) en-keyword=PNGase: peptide:N-glycanase kn-keyword=PNGase: peptide:N-glycanase en-keyword=PTC: plant complex type kn-keyword=PTC: plant complex type en-keyword=RCA120: Ricinus communis agglutinin (120 kDa) kn-keyword=RCA120: Ricinus communis agglutinin (120 kDa) en-keyword=RP-HPLC: reversed-phase HPLC kn-keyword=RP-HPLC: reversed-phase HPLC en-keyword=SF-HPLC: size-fractionation HPLC kn-keyword=SF-HPLC: size-fractionation HPLC en-keyword=Xyl: D-xylose kn-keyword=Xyl: D-xylose en-keyword=affinity chromatography kn-keyword=affinity chromatography en-keyword=enzyme assay kn-keyword=enzyme assay en-keyword=free N-glycans kn-keyword=free N-glycans en-keyword=transgenic plant kn-keyword=transgenic plant END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=1 article-no= start-page=8239 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=201708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synthesis of Sulfo-Sialic Acid Analogues: Potent Neuraminidase Inhibitors in Regards to Anomeric Functionality en-subtitle= kn-subtitle= en-abstract= kn-abstract= The design, synthesis and application of N-acetylneuraminic acid-derived compounds bearing anomeric sulfo functional groups are described. These novel compounds, which we refer to as sulfo-sialic acid analogues, include 2-decarboxy-2-deoxy-2-sulfo-N-acetylneuraminic acid and its 4-deoxy-3,4-dehydrogenated pseudoglycal. While 2-decarboxy-2-deoxy-2-sulfo-N-acetylneuraminic acid contains no further modifications of the 2-deoxy-pyranose ring, it is still a more potent inhibitor of avian-origin H5N1 neuraminidase (NA) and drug-resistant His275Tyr NA as compared to the oxocarbenium ion transition state analogue 2,3-dehydro-2-deoxy-N-acetylneuraminic acid. The sulfo-sialic acid analogues described in this report are also more potent inhibitors of influenza NA (up to 40-fold) and bacterial NA (up to 8.5-fold) relative to the corresponding anomeric phosphonic acids. These results confirm that this novel anomeric sulfo modification offers great potential to improve the potency of next-generation NA inhibitors including covalent inhibitors. en-copyright= kn-copyright= en-aut-name=VavrickChristopher J. en-aut-sei=Vavrick en-aut-mei=Christopher J. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MutoChiaki en-aut-sei=Muto en-aut-mei=Chiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HasunumaTomohisa en-aut-sei=Hasunuma en-aut-mei=Tomohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ArakiMichihiro en-aut-sei=Araki en-aut-mei=Michihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=WuYan en-aut-sei=Wu en-aut-mei=Yan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=GaoGeorge F. en-aut-sei=Gao en-aut-mei=George F. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OhruiHiroshi en-aut-sei=Ohrui en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=IzumiMinoru en-aut-sei=Izumi en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KiyotaHiromasa en-aut-sei=Kiyota en-aut-mei=Hiromasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Science, Technology and Innovation, Kobe University kn-affil= affil-num=4 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil=Graduate School of Science, Technology and Innovation, Kobe University kn-affil= affil-num=6 en-affil=CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences kn-affil= affil-num=7 en-affil=CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences kn-affil= affil-num=8 en-affil=Yokohama College of Pharmacy kn-affil= affil-num=9 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=10 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue= article-no= start-page=5 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ginkgo biloba α-fucosidase with activity towards plant complex type N-glycans containing the Lewis a epitope: Purification and characterization en-subtitle= kn-subtitle= en-abstract=　銀杏種子から高分子量 （SDS-PAGE で120 kDa） を有し，α-フコース含有オリゴ糖に活性を示すα-フコシダーゼ（α-fucosidase Gb）を均一に精製した．ルイス a エピトープ含有 N-グリカンを基質とした場合，α-fucosidase Gb の至適 pH は 5.5 付近であることから，本酵素は液胞のような酸性環境で機能していることが示唆された．N‒末端アミノ酸配列が化学修飾のため同定できなかったため，本酵素が GH29 ファミリーに属するかどうかは不明である．α-Fucosidase Gb は，Lacto-N-fucopentaose IIIの α1,3-フコース残基やルイス a エピトープ含有の植物複合型N-グリカンのα1,4-フコース残基を加水分解することから，α-フコース含有オリゴ糖やN 型糖タンパク質の分解プロセスに関与することが示唆された． kn-abstract=　We have identified, and purified to homogeneity, a high molecular weight Ginkgo biloba α-fucosidase (α-fucosidase Gb, 120 kDa estimated by SDS‒PAGE) with activity against α-fucosylated oligosaccharides. When a Lewis a epitope-containing N-glycan was used as a substrate, α-fucosidase Gb showed optimum activity at approximately pH 5.5, suggesting that it functions in acidic environments such as the vacuole. It remains uncertain, however, whether this Ginkgo α-fucosidase belongs to the GH29 family, since its N-terminal sequence could not be determined, probably due to a chemical modification. α-Fucosidase Gb showed substantial activity towards the α1,3-fucosyl linkage in Lacto-N-fucopentaose III and an α1,4-fucosyl linkage in the Lewis a epitope found in plant complex type N-glycans, indicating an involvement in the degradation process of α-fucosylated oligosaccharides or N-glycoproteins. en-copyright= kn-copyright= en-aut-name=ItanoSatsuki en-aut-sei=Itano en-aut-mei=Satsuki kn-aut-name=板野紗月 kn-aut-sei=板野 kn-aut-mei=紗月 aut-affil-num=1 ORCID= en-aut-name=MaedaMegumi en-aut-sei=Maeda en-aut-mei=Megumi kn-aut-name=前田恵 kn-aut-sei=前田 kn-aut-mei=恵 aut-affil-num=2 ORCID= en-aut-name=Md. Ziaur Rahman en-aut-sei=Md. Ziaur Rahman en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=4 ORCID= affil-num=1 en-affil=Graduate School of Environmental and life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=2 en-affil=Graduate School of Environmental and life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=3 en-affil=Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission kn-affil= affil-num=4 en-affil=Graduate School of Environmental and life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 en-keyword=α-fucosidase kn-keyword=α-fucosidase en-keyword=plant N-glycan kn-keyword=plant N-glycan en-keyword=N-glycan degradation kn-keyword=N-glycan degradation en-keyword=Ginkgo biloba kn-keyword=Ginkgo biloba END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page=1 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=大豆発芽時期におけるグリシニン分解酵素の活性変動 kn-title=Changes in Glycinin‒Digesting Protease Activity During Soybean Germination. en-subtitle= kn-subtitle= en-abstract=　大豆発芽期におけるグリシニン分解酵素 (98 kDa SBP) の活性変動を解析した．大豆種子を４時間水で膨潤後， 25 ℃ 暗黒下で発芽させた．経時的にサンプリングを行い，2M NaCl を含むトリス緩衝液 (㏗ 7.0) により粗酵素を抽出 後，グリシニン由来のトリプシン分解ペプチドを基質としてグリシニン分解酵素の活性変動を逆相 HPLC により追 跡した．その結果，種子膨潤後４日間比活性はほぼ一定の値を保ち，以後徐々に低下することが分かった．次いで， 粗酵素溶液からイオン交換 HPLC により98 kDa SBP を部分精製するとともに，発芽期における 98 kDa SBP の消長 を解析したところ，98 kDa SBP は乾燥種子及び各発芽段階の種子中全てに認められ，かつグリシン分解活性もグリ シニン由来のトリプシン分解ペプチド基質に対する活性と同様に認められた．以上の結果から，98 kDa SBP は種子 発芽に伴い誘導されるプロテアーゼではなく，種子貯蔵型のプロテアーゼであることが明らかになった． kn-abstract=　Changes in glycinin-digesting protease activity during soybean germination have been investigated. The glycinin-digesting protease activities of imbibed or germinated soybean seed were assayed by RP‒HPLC using a tryptic peptide from CM‒glycinin or by SDS‒PAGE using CM‒glycinin as the endogenous substrate. Proteolytic activities of the germinated soybean seeds were found through the whole period of germination, the activities were maintained significantly unchanged during germination for 4 days, and then those specific activities declined slowly. AE‒HPLC analysis of the glycinin-digesting protease in the imbibed or germinated soybean seeds showed unchanged peaks corresponding to glycinin- digesting activity, suggesting that the glycinin-digesting protease was not induced during germination but had already been synthesized during seed maturation. en-copyright= kn-copyright= en-aut-name=Md. Akhtaruzzaman en-aut-sei=Md. Akhtaruzzaman en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MaedaMegumi en-aut-sei=Maeda en-aut-mei=Megumi kn-aut-name=前田恵 kn-aut-sei=前田 kn-aut-mei=恵 aut-affil-num=2 ORCID= en-aut-name=KitagawaKeiko en-aut-sei=Kitagawa en-aut-mei=Keiko kn-aut-name=北川恵子 kn-aut-sei=北川 kn-aut-mei=恵子 aut-affil-num=3 ORCID= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=4 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Plant protease kn-keyword=Plant protease en-keyword=glycinin kn-keyword=glycinin en-keyword=germination kn-keyword=germination en-keyword=Glycine max kn-keyword=Glycine max END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=4 article-no= start-page=1111 end-page=1114 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Evidence for new beta 1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera en-subtitle= kn-subtitle= en-abstract= kn-abstract=In a previous study (Y. Kimura et al., Biosci. Biotechnol Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Ga1 beta 1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new beta 1-3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such beta 1-3galactosyltransferase activity, which can transfer galactosyl residues with the beta 1-3 linkage to beta 1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc(2)GlcNAc(2)Man(3)-GlcNAc(2)-PA as acceptor N-glycan, we detected the beta 1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique beta 1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland. en-copyright= kn-copyright= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SakamuraSho en-aut-sei=Sakamura en-aut-mei=Sho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UshijimaTakayuki en-aut-sei=Ushijima en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HamaYoichiro en-aut-sei=Hama en-aut-mei=Yoichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KajiuraHiroyuki en-aut-sei=Kajiura en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=FujiyamaKazuhito en-aut-sei=Fujiyama en-aut-mei=Kazuhito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OkiharaKiyoshi en-aut-sei=Okihara en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HashimotoKen en-aut-sei=Hashimoto en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=SugimotoHiroyuki en-aut-sei=Sugimoto en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YamadaHideo en-aut-sei=Yamada en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Department of Biofunctional Chemistry, Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Department of Biofunctional Chemistry, Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Department of Biofunctional Chemistry, Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Department of Applied Biological Sciences, Faculty of Agriculture, Saga University affil-num=5 en-affil= kn-affil=The International Center for Biotechnology, Osaka University affil-num=6 en-affil= kn-affil=The International Center for Biotechnology, Osaka University affil-num=7 en-affil= kn-affil=Yamada Apiculture Center, Inc. affil-num=8 en-affil= kn-affil=Yamada Apiculture Center, Inc. affil-num=9 en-affil= kn-affil=Yamada Apiculture Center, Inc. affil-num=10 en-affil= kn-affil=Yamada Apiculture Center, Inc. en-keyword=royal jelly glycoprotein; N-glycan kn-keyword=royal jelly glycoprotein; N-glycan en-keyword=Thomsen-Friedenreich antigen (Gal beta 1-3GalNAc) kn-keyword=Thomsen-Friedenreich antigen (Gal beta 1-3GalNAc) en-keyword=beta 1-3 galactosyltransferase kn-keyword=beta 1-3 galactosyltransferase en-keyword=Apis mellifera kn-keyword=Apis mellifera END start-ver=1.4 cd-journal=joma no-vol=11 cd-vols= no-issue= article-no= start-page=74 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19891215 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=編集後記 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=1 article-no= start-page=7 end-page=13 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19960201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A Spectroscopic Behavior of Zeaxanthin Molecular Aggregate and Its From kn-title=Zeaxanthin分子集合体の分光学的挙動とその形態 en-subtitle= kn-subtitle= en-abstract=我々はxanthophyllの一つであるluteinが種々の水溶液例えばたんぱく質溶液、うすいアセトン溶液、うすい界面活性剤水溶液、卵黄レシチン製リポソーム、あるいはホウレンソウdigalactosyl diglyceride製リポソームに分散溶解してキラルならせん状集合体を形成し、可視領域に光学活性を示すと同時に左巻きのらせん構造体を作ることを既に明らかにしている。本報告ではluteinの構造異性体であるzeaxanthinを用いて同様に分子集合体を形成するかどうかを検討した。zeaxanthinをsodiumdodecylsulfate (SDS)に分散させると、円2色性(CD)スペクトルが逆のCotton効果を与え、またそのスペクトルパターンが逆転するSDS濃度がluteinのとき0.6mMであったのに対して約3.51mMと高く、またそのときのSDS結合量も多いという相異はあるが水系においてπ*-π*相互作用行わせるような分子集合体を形成するという基本的挙動を示した。PCリポソームに分散させたときアルカリ性でCa2+存在下らせん構造を与える点もluteinと同じであった。しかし、用いたzeaxanthinが合成された光学異性体混合物であったためか左巻きと右巻きらせんの両方とも観察されたホウレンソウlutein Aの左巻きらせんだけの結果と異なった。以上の結果からzeaxanthinもluteinと同じく水系で分子集合体を形成するという基本的分子挙動をとることが明らかとなった。 kn-abstract=Lutein, one of the xanthophylls, can be dispersed in various aqueous solutions, for example, protein solution, dilute acetone solution, dilute sodium dodecyl sulfate (SDS) solution, dodecyl trimethylammonium bromide solution, hen egg yolk phosphatidyl-choline (PC) liposome, or digalactosyldiglyceride liposome, to from chiral helical aggregates which acquire an optical activity in the visible region, and have a left handed helical structure. In this report, zeaxanthin, a structural isomer of lutein, was subjected to investigation as to whether zeaxanthin formed the chiral helical aggregate. When zeaxanthin was dispersed in SDS solution, some differences from lutein were obserbed. (1) CD spectrum of positive Cotten effect was shown, which was the reverse of lutein. (2) SDS concentration giving the reversion of the CD spectrum pattern was higher (3.5mM) than that of lutein (0.6mM). IN spite of these differences, however, the results showed that zeaxanthin molecules also associated to from molecular aggregate in such a manner performing π*-π*interaction at conjjugated polyene sites. zeaxanthin dispersed in PC liposome gave both left and right-handed helical structure under presence of Ca2+ at alkaline condition in comparison with lutein's left-handed structure. This result may depend on the fact that synthesized racemic zeaxanthin is subjected to this experiment. Those results gave the general conclusion that xanthophylls had the same basic types of behavior for making molecular aggregate in aqueous dispersion. en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= en-aut-name=ItaniJunko en-aut-sei=Itani en-aut-mei=Junko kn-aut-name=伊谷淳子 kn-aut-sei=伊谷 kn-aut-mei=淳子 aut-affil-num=2 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=3 ORCID= en-aut-name=TakedaKunio en-aut-sei=Takeda en-aut-mei=Kunio kn-aut-name=竹田邦雄 kn-aut-sei=竹田 kn-aut-mei=邦雄 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山理科大学理学部 en-keyword=zeaxanthin kn-keyword=zeaxanthin en-keyword=molecular aggregate kn-keyword=molecular aggregate en-keyword=chiral helical structure liposome kn-keyword=chiral helical structure liposome END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=1 article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=19950201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Absorption and Cleavage Spinach Carotenoids in Inverted Quail Intestine and Transfer of the Carotenoids into Egg Yolk kn-title=ウズラ反転小腸によるホウレンソウ葉カロテノイドの吸収とレチノイド生成及び血中カロテノイドの卵黄への移行機作について en-subtitle= kn-subtitle= en-abstract=ホウレンソウ葉カロテノイドをウズラ反転小腸とインキュベートするとβ-カロテンは素早く吸収されてレチノールに変化し、粘膜外に移行する。ルテインをはじめとするキサントフィルは小腸に吸収された後β-カロテン開裂酵素の作用をあまり受けずに粘膜を通過するがその吸収と移行の速度はキサントフィルの種類によって大きく異なり、ルテイン、アンセラキサンチン、ビオラキサンチンの順である。さらに、吸収されたキサントフィルのうちに主にルテインは血清LDLに取り込まれて卵黄に移行蓄積されることを明らかにした。 kn-abstract=At incubation with spinach carotenoid of inverted quail intestine,β-carotene was quickly absorbed into the intestinal mucosa and then changed to retinol,passing out of the mucosa.The majority of the xanthophyll including lutein,after absorption into the mucosa,passed out of the mucosa in intact form,and the velocities varied per xanthophylls in descending order of lutein,antheraxanthin,and violaxanthin.It became apparent that out of the absorbed xanthophylls,chiefly lutein was incorporated into quail serum LDL,where it was transported into the egg yolk and accumulated. en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= en-aut-name=MikiAkira en-aut-sei=Miki en-aut-mei=Akira kn-aut-name=三木晃 kn-aut-sei=三木 kn-aut-mei=晃 aut-affil-num=2 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=3 ORCID= en-aut-name=SatohKatsunori en-aut-sei=Satoh en-aut-mei=Katsunori kn-aut-name=佐藤勝紀 kn-aut-sei=佐藤 kn-aut-mei=勝紀 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=β-carotene kn-keyword=β-carotene en-keyword=lutein kn-keyword=lutein en-keyword=low density lipoprotein kn-keyword=low density lipoprotein END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=35 end-page=41 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒマ種子カルス化に伴うアスパラギン結合型糖鎖の構造変化 kn-title=Changes in Asparagine-linked Sugar Chains of Glycoproteins in Ricinus communis Seeds during Callus Induction en-subtitle= kn-subtitle= en-abstract=植物細胞の脱分化と糖蛋白質糖鎖（アスパラギン結合型糖鎖）の発現機構との相関を明らかにすることを目的とし、ヒマ種子から誘導したカルス中に発現されるアスパラギン結合型糖鎖（N-グルカン）の構造解析を行った。2,4-D処理によるカルス化誘導を行い、経時的に採取した組織からTriton X-100を含むHEPES緩衝液（ph7.4)で全糖蛋白質を抽出した。得られた糖蛋白質からヒドラジン分解によりN-グリカンを遊離された後、N-アセチル化、ピリジルアミン(PA)化により蛍光標識糖鎖を調製した。逆相およびサイズフラクショネーションHPLCによりPA-糖鎖を単一に精製後、糖鎖2次元マップ法により構造の同定を行った。その結果、カルス化に伴い、ハイマンノース型糖鎖(Man7-4GlcNAc2)の相対量が顕著に減少するのに対して、キシロース/フコース含有型糖鎖( GlcNAclMan3FuclXyllGlcNAc2)の相対量が増加することが明らかになった。この現象は、植物細胞の脱分化に伴い、糖鎖プロセシングに関与するα-マンノシダーゼあるいはN-アセチルグリコサミン転移酵素の活性化が起こることを示唆するものと考えられる。 kn-abstract=Using glycotechnology procedures, structural changes in asparagine-linked sugar chains (N-glycans) of glycoproteins in Ricinus communis seeds during dedifferentiation (callus induction) have been explored. N-Glycans were released from the glycoproteins in the 2,4-D derived callus tissues by hydrazinolysis, and the resulting oligosaccharides were N-acetylated and coupled with 2-aminopyridine. Structures of the purified pyridylaminated (PA-) N-glycans could be deduced by two-dimensional (2D) sugar chain mapping method. Structural analysis clearly showed that the relative amount of high-mannose type N-glycans of the endospermic glycoproteins decreased as the plant cells dedifferentiated, while that of complex type N-glycans increased. this observation suggested that enhancement of expression and/or activation of certain α-mannosidase(s) involved in N-glycan processing could occur during dedifferentiation of plant cells. en-copyright= kn-copyright= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=1 ORCID= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=N-glycan structure kn-keyword=N-glycan structure en-keyword=plant glycoprotein kn-keyword=plant glycoprotein en-keyword=callus induction kn-keyword=callus induction en-keyword=Ricinus communis kn-keyword=Ricinus communis END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=29 end-page=34 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Establishment of an Assay Method for βーCarotene-15,15'-Dioxygenase of Rat lntestine Mucosa and lts Enzymatic Properties kn-title=ラット小腸粘膜β-carotene-15,15'-dioxygenase の活性測定法の確立と酵素化学的性質 en-subtitle= kn-subtitle= en-abstract=βーカロテンの15,15'位における中央開裂はβーカロテンの吸収・代謝において確立された説となっていない。それは分子端からの逐次開裂も示唆されているからである。このもんだいはin vitroにおけるβーcarotene-15, 15'dioxygenase (BCDO)活性測定法が不完全なために起こったのではないかと考え、補因子活性をそれ以外の測定条件と共に検討し、測定方法の確立を目指した。ラット小腸粘膜ホモジェネートのBCDO活性はGoodmanらの既知の方法によっては検出出来なかった。SH試薬(GSH,またはDTT)及びニコチンアミド(NA)はBCDO活性測定に必要であった。NAはNADHに置き換えられるが、生成物はレチナールの代わりにレチノールであった。NAD+はBCDOの活性を一部阻害する。他の補因子の最適濃度をこれらの条件下で決め、1 mM GSH, 1 mM Fe+, 10 mM glycocholateとした。これらの結果から、in vitro におけるBCDO活性測定が再現性良く行われることになり、Goodmanらによって提示されたβーカロテンの中央開裂が確認できた。 kn-abstract=A central cleavage at 15, 15' position of　βーcarotene has not been an established theory in absorption and metabolism, because an exenteric cleavage of βーcarotene for producing apocarotenals has been suggested. We considered that this problem was based only on the imperfection of the assay method for βーcarotene-15,15'-dioxygenase (BCDC) activity in vitro, and tried the establishment of this assay method by investigating cofactor activities together with other assy conditions. BCDO activity of rat intestina mucosa homogenate could not be detected with the known method by Goodman et al. SH reagents (GSH or DTT) and nicotinamide (NA) were essential for BCDO assay. NADH could take place with NA, but the product was retinol instead of retinal. NAD+ partially inhibited the enzyme activity. Optimum concentrations of other cofactors were decided under the following conditions : 1 mM GSH, 1 mM Fe2+, and 10 mM glycocholate. From these results, a good reproducibility of the in vitro assay for BCDO activity was obtained, and it was confirmed that the central cleavage theory presented by Goodman was reasonable. en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= en-aut-name=WatanabeMasanori en-aut-sei=Watanabe en-aut-mei=Masanori kn-aut-name=渡辺正徳 kn-aut-sei=渡辺 kn-aut-mei=正徳 aut-affil-num=2 ORCID= en-aut-name=NakajimaAkiko en-aut-sei=Nakajima en-aut-mei=Akiko kn-aut-name=中島明夫 kn-aut-sei=中島 kn-aut-mei=明夫 aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=βーcarotene cleavage enzyme kn-keyword=βーcarotene cleavage enzyme en-keyword=assay method kn-keyword=assay method en-keyword=rat intestine mucosa kn-keyword=rat intestine mucosa END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=19 end-page=24 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=タケノコ（ハチク）の中に存在する遊離N-グリカンの構造解析 kn-title=Structural Analysis of Free N-Glycans in Bamboo (Phyllostachys heterocycla) Shoots en-subtitle= kn-subtitle= en-abstract=植物細胞の分化・成長における遊離N-グリカンの植物ホルモン様活性が推定されている。そこで、分化・成長中の細胞における遊離N-グリカンの偏在性を立証する研究の一環として、成長速度の速いことで知られるタケノコから遊離N-グリカンを精製し、それらの製造解析を行った。タケノコ抽出物の透析外液から調整したオリゴ糖画分を2-アミノピリジンで蛍光標識した後、遊離N-グリカンを逆相HPLC、ConAアフィニティークロマト及びサイズフラッシュネーションHPLCにより精製した。得られた遊離N-グリカンの構造解析はα-マンノシダーゼ消化、糖鎖2次元マップ法、IS-MS及びMS/MS分析を組み合わせることにより行った。その結果、タケノコ中にはエンド-β-N-アセチルグリコサミニダーゼにより誘導されたと考えられるハイマンノース型糖鎖（Man8-5GlcNAc1、2.6nmol/gram fresh weight）が存在することが明らかになった。エンドウ胚軸同様、ハイマンノース型糖鎖の存在量が植物複合型糖鎖の存在量に比べかなり高いことから、前者構造の遊離糖鎖が植物細胞の分化・成長において何らかの重要な生理機能を担う可能性も考えられる。 kn-abstract=Free N-glycans, the high-mannose-type and the plant complex-type, have been found in bamboo shoots. These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con A-Sepharose affinity chromatography, reversed-phase HPLC, and size-fractionation HPLC. The structures of these pyridylaminated free N-glycans were identified by two-dimensional sugar chain mapping, exomannosidase digestions, and ion-spray tandem mass spectrometry. The structual analyses showed that the various free high-mannose type sugar chains having one GlcNAc(Man8-5GlcNAc1) and free xylose/fucose containing sugar chains having the chitobiose segment occur in the developing bamboo shoots, suggesting that an endo-β-N-acetylglucosaminidase should produce the former structures, and a peptide: N-glycanase should produce the latter structures. en-copyright= kn-copyright= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=1 ORCID= en-aut-name=UeyamaTatsuya en-aut-sei=Ueyama en-aut-mei=Tatsuya kn-aut-name=上山達也 kn-aut-sei=上山 kn-aut-mei=達也 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=free N-glycan kn-keyword=free N-glycan en-keyword=plant N-glycan kn-keyword=plant N-glycan en-keyword=endo-β-N-acetylglucosaminidase kn-keyword=endo-β-N-acetylglucosaminidase en-keyword=peptide:N-glycanase kn-keyword=peptide:N-glycanase en-keyword=Phyllostachys heterocycla kn-keyword=Phyllostachys heterocycla END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=13 end-page=17 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Isolation and Purification of Nocardamine from the Validamycin Producer kn-title=バリダマイシン生成菌培養液から抗細菌性抗生物質ノカルダミンの分離・精製 en-subtitle= kn-subtitle= en-abstract=Streptomyces hygroscopicus subsp. limoneus はバリダマイシン生成菌として知られているが、その抗カビ物質以外に抗細菌性抗生物質を生成することは知られていない。われわれはその微生物がバリダマイシン非生成条件において生成する抗細菌性抗生物質を見出そうと試みた。その結果、グラム陰性細菌に活性を示す抗生物質EO-3を得たのでこれを分離・精製してノカルダミンと同定した。本報告では本抗生物質の培養条件、抗菌スペクトル、精製および同定方法について述べる。尚、この研究は平成6年度から8年度までの3年間に亙る岡山大学学内特定研究『特殊環境生物の機能開発と物質生産への応用』を分担して行ったものである。 kn-abstract=An antibacterial active against P. mirabilis was isolated from the culture of Streptomyces hygroscopicus subsp. limoneus, validamycin producer. The antibiotic was found to be produced with a non-validamycin producing condition. The antibiotic was identified as nocardamine with the analytical data, IR, 1H-NMR and 13C-NMR spectra. en-copyright= kn-copyright= en-aut-name=HigashideEiji en-aut-sei=Higashide en-aut-mei=Eiji kn-aut-name=東出栄治 kn-aut-sei=東出 kn-aut-mei=栄治 aut-affil-num=1 ORCID= en-aut-name=OmatsuYoshiharu en-aut-sei=Omatsu en-aut-mei=Yoshiharu kn-aut-name=大松義治 kn-aut-sei=大松 kn-aut-mei=義治 aut-affil-num=2 ORCID= en-aut-name=InoueSuemi en-aut-sei=Inoue en-aut-mei=Suemi kn-aut-name=井上末瑞 kn-aut-sei=井上 kn-aut-mei=末瑞 aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=4 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=5 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Streptomyces kn-keyword=Streptomyces en-keyword=antibacterial compoud kn-keyword=antibacterial compoud en-keyword=nocardamine kn-keyword=nocardamine en-keyword=non-validamycin producing condition kn-keyword=non-validamycin producing condition END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=1 article-no= start-page=9 end-page=14 dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=200002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=登熟期ヒマ種子ミクロゾーム画分からのアリル-α-マンノシダーゼの精製と酵素学的諸性質 kn-title=Purification and Properties of Aryl-α-mannosidase from Microsomal Fraction of Developing Ricinus communis Endosperms en-subtitle= kn-subtitle= en-abstract=登熟期ヒマ種子のミクロゾーム画分からp-nitrophenyl-α-D-mannopyranoside(PNP-α-Man)及び蛍光標識N-グリカンに対して活性を示すα-マンノシダーゼを精製後、酵素学的諸性質を解析した。精製酵素は還元条件下のSDS-PAGEでは43kDaの相対分子量を示し、PNP-α-Manを基質とした場合の至適反応条件は50-60℃、pH4.5-5.0であった。本酵素はEDTAにより阻害を受けたが、Zn2+及びCa2+添加により活性が回復することから金属イオン要求性の酵素であると思われる。また、PNP-α-Manに対するKm値は1.3mM、Man5GLcNAc2-PAに対するKm値は0.4mMであった。本酵素はMan5GlcNAc2-PAに作用しMan4GlcNAc2-PAを誘導するものの、Man6GlcNAc2-PA対してむしろ強い活性を示し、Man5GlcNAc2-PA(88%)及びMan4GlcNAc2-PA(12%)を誘導した。しかしながら、Man4GlcNAc2-PA,GlcNAc1Man5GlcNAc2-PA、Man4Xy11GlcNAc2-PAは本酵素の有効な基質とはなり得なかった。これらの結果から、本酵素はα-1,2-マンノース残基を優先的に加水分解し、その後、β-結合マンノースに結合するα-1,3マンノース残基を遊離することが示唆された。 kn-abstract=An α-mannosidase, which would be involved in N-linked glycoprotein metabolism, was purified and characterized from microsomal fraction of developing Ricinus communis endosperms. The purified enzyme with 43 kDa on SDS-PAGE showed maximal activity at pH5.0 and 50℃, when p-nitrophenyl-α-mannopyranoside was used as a substrate. α-Mannosidase activity was inhibited by EDTA and the reduced activity was rescued by addition of Zn2+ or Ca2+, suggesting this α-mannosidase should be a metal enzyme. Ricinus aryl-α-mannosidase was able to convert the Man6GlcNAc2-PA and Man5GlcNAc2-PA to Man4GlcNAc2-PA but was completely inactive toward Man4GlcNAc2-PA, Man4Xy11GlcNAc2-PA and GlcNAc1Man5GlcNAc2-PA. en-copyright= kn-copyright= en-aut-name=YamaiMasafumi en-aut-sei=Yamai en-aut-mei=Masafumi kn-aut-name=山井雅文 kn-aut-sei=山井 kn-aut-mei=雅文 aut-affil-num=1 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉信 kn-aut-sei=木村 kn-aut-mei=吉信 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=plant α-mannosidase kn-keyword=plant α-mannosidase en-keyword=plant glycoprotein kn-keyword=plant glycoprotein en-keyword=N-glycan metabolism kn-keyword=N-glycan metabolism END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=1 article-no= start-page=15 end-page=18 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=200102 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=植物複合型遊離Ｎ－グリカンが細胞壁に局在する可能性 kn-title=The Possibility that Plant Complex Type FreeN-Glycans Localize in Cell Wall Fraction en-subtitle= kn-subtitle= en-abstract=登熟期銀杏種子の細胞壁画分に局在する遊離型-グリカンの構造解析を行った．登熟期銀杏種子抽出物を徹底的に透析して細胞質中に存在する遊離-グリカンを除去した後，細胞壁画分を遠心分離により回収した．得られた細胞壁画分を希酸処理（0.01NHCl/20メタノール中で100℃，30分間処理）した後，透析によりオリゴ糖鎖を透析外液に回収した．オリゴ糖鎖画分を２-アミノピリジンで蛍光標識した後，遊離-グリカンをゲルろ過およびサイズフラクショネーションHPLCにより精製した。遊離-グリカンの構造解析は，IS-MS分析，α-1，2-マンノシダーゼ消化，および糖鎖２次元マップ法を組み合わせることにより行った．その結果，細胞壁画分からは，ハイマンノース型および植物複合型構造を有する遊離-グリカンが検出されたが，細胞質画分には74pmol/freshweightofseed（全遊離グリカンの３構相当）程度の存在量であった植物複合型遊離-グリカンが，細胞壁画分では310pmol/freshweightofseed（全遊離グリカンの40相当）程度の濃度を占めることが明らかとなった。この結果は，植物複合型し造の遊離-グリカンは主に細胞壁画分に局在し，水素結合あるいは疎水結合により細胞壁マトリックスに強固に結合とていることを示唆するものと考えられる kn-abstract=In this report, we bring up the possibility that complex type free N-glycans may localize in cell wall fraction of developing seeds (Ginkgo biloba seeds). Several free N-glycans extracted by mild acid hydrolysis of cell wall fraction were coupled with 2-aminopyridine and purified by gel filtration, size-fractionation HPLC, and reversed-phase HPLC. The structures of the pyridylaminated free N-glycans were identified by two-dimensional sugar chain mapping, α-1,2-mannosidase digestions, and ionspray tandem mass spectrometry. The structural analyses showed that highmannose type free N-glycans having one GlcNAc residue (Man8-5GlcNAc1) and plant complex type free N-glycans having the N-acetyl chitobiose unit also occur in the call wall fraction of the developing Ginkgo seeds. However, quantitative analysis of such free N-glycans showed that the plant complex type free glycans found in small amounts(～3%) in the cytosolic fraction accounted for nearly 40% of total free N-glycans, This observation suggested that the complex type free N-glycans might occur and localized in the cell wall fraction. en-copyright= kn-copyright= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=1 ORCID= en-aut-name=SuzukiMasashi en-aut-sei=Suzuki en-aut-mei=Masashi kn-aut-name=鈴木将史 kn-aut-sei=鈴木 kn-aut-mei=将史 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=free N-glycan kn-keyword=free N-glycan en-keyword=plant N-glycan kn-keyword=plant N-glycan en-keyword=localization of free N-glycan kn-keyword=localization of free N-glycan en-keyword=cell wall fraction kn-keyword=cell wall fraction en-keyword=Ginkgo biloba kn-keyword=Ginkgo biloba END start-ver=1.4 cd-journal=joma no-vol=81 cd-vols= no-issue=1 article-no= start-page=1 end-page=7 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=1993 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A New Assay Method of Retinal Reductase Activity in Rat Intestinal Mucosa and Its Enzymatic Properties kn-title=ラット小腸粘膜レチナール還元酵素活性の測定法の確立と酵素化学的性質 en-subtitle= kn-subtitle= en-abstract= kn-abstract=ラット小腸粘膜のレチナール還元酵素(RRase)のin vitroにおける活性測定法を確立すると共に,その系におけるレチナールの還元及び酸化反応について調べた.小腸のRRaseは摂取されたβ-カロテンなどプロビタミンAから生じるレチナールをレチノールに変える作用が主たる役割と考えられる酵素である.小腸粘膜ホモジェネートを用いてin vitroでRRase活性を測定すると,比活性の再現性が乏しく,またホモジェネート中でのRRaseの速やかな失活が起こる.これを防ぐためには,酵素調製用緩衝液にニコチンアミドやNADHなどのニコチンアミド誘導体を2mM以上加えるのが有効であることを見いだした.最適pHは4.3,cofactorとしてGSHを要求する点はすでにGoodmanらが報告している通りであるが,GSH以外のチオール試薬もGSHと同様に有効であった.EDTA添加によって活性を殆ど失うが,Ca2+,Mg2+の添加で活性が回復することから,これらが金属イオンとして要求されていると思われる.RRaseのkm値は83.3μMであり,200μM以上のレチナールを用いて活性測定を行うと120minで基質レチナールの40%以上が還元される.このことは,レチナール還元反応がレチノール生成に大きく傾いた反応であることを示すものである。 en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= en-aut-name=FujiiYuko en-aut-sei=Fujii en-aut-mei=Yuko kn-aut-name=藤井佑子 kn-aut-sei=藤井 kn-aut-mei=佑子 aut-affil-num=2 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=80 cd-vols= no-issue=1 article-no= start-page=7 end-page=15 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of Spinach Carotenoids on Differentiation of Some Human Leukemia Cell Lines kn-title=ホウレンソウ葉カロテノイドがヒト白血病細胞株の分化に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=ホウレンソウ緑葉カロテノイドのヒト骨髄性白血病細胞株に対する分化誘導活性を調べると同時に,その目的に合致する活性測定法の選択,及び細胞株の選択を行った. ホウレンソウ葉カロテンはML-1,ML-2及びHL-60細胞の分化誘導活性を示し,ルテイン,ビオラキサンチン,ネオキサンチンのキサントフィル類は活性を示さなかった.ホウレンソウ葉カロテンは細胞の生存率を80-85%に低下させるが,キサントフィルの毒性は極めて弱い.そのカロテンの分化誘導活性はレテインとの共集合体を作らせることによって増加しており,これはカロテンと細胞との相互作用の増加を示すものである.カロテノイドを用いた場合の分化誘導活性測定法としてはNBT還元法と間接蛍光抗体法が有用であった.試験細胞として上記3細胞株はいずれも有用であるが,なかでもHL-60はホウレンソウ葉カロテンによる分化誘導活性を最も強く受ける。 en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= en-aut-name=HizumeKazuhisa en-aut-sei=Hizume en-aut-mei=Kazuhisa kn-aut-name=樋詰和久 kn-aut-sei=樋詰 kn-aut-mei=和久 aut-affil-num=2 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=3 ORCID= en-aut-name=MinowadaJun en-aut-sei=Minowada en-aut-mei=Jun kn-aut-name=蓑和田潤 kn-aut-sei=蓑和田 kn-aut-mei=潤 aut-affil-num=4 ORCID= en-aut-name=MatsuoYoshinobu en-aut-sei=Matsuo en-aut-mei=Yoshinobu kn-aut-name=松尾良信 kn-aut-sei=松尾 kn-aut-mei=良信 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=林原(株)生物化学研究所藤崎細胞センター affil-num=5 en-affil= kn-affil=林原(株)生物化学研究所藤崎細胞センター END start-ver=1.4 cd-journal=joma no-vol=78 cd-vols= no-issue=1 article-no= start-page=1 end-page=9 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=1991 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Absorption and Accumulation of Carotenoid and Retinoid Production in Rat kn-title=β-Carotene及びホウレンソウカロテノイドのラットにおける吸収と蓄積 en-subtitle= kn-subtitle= en-abstract= kn-abstract=植物性食品に広く含まれるカロテノイドのうち,β-caroteneなどはプロビタミンA効果以外に抗ガン,抗酸化防止効果などが注目されているが,カロテノイドの消化・吸収機構及び体内分布に関する基礎的知見は少ない,カロテノイド非蓄積動物と呼ばれるラットについてβ-carotene単独投与及びホウレンソウカロテノイドとを投与し,それぞれにおける各カロテノイドの吸収挙動と吸収カロテノイド及び生成retinolの体内存在状態を調べた.β-caroteneは単独に投与すると約50%の吸収率となるが,xanthophyll共存下では数%にまで低下する.これはβ-caroteneの吸収をxanthophyllが妨害していることを示す.β-caroteneは肝臓にもっとも多く蓄積されneoxanthinがこれを次いでいる.もっとも多く吸収されたluteinは肝臓にはほとんど検出されず,血液中に少量存在していた.このような両カロテノイドの挙動はカロテノイド非蓄積動物に特徴的なものと考えられる.カロテノイドを含まない飼料を投与すると一時的にretinolの血中濃度は上昇し,肝濃度は低下するが,カロテノイドを投与すると恒常的血中retinol濃度に速やかに戻る.β-carotene及びホウレンソウカロテノイドを25日間投与したときのretinolの正味増加量はそれぞれ474μgと522μgであるが血中のretinolレベルはほとんど変化していない.このことは肝に充分量のretinol存在しておれば血中retinolレベルはほぼ一定に保たれる制御作用のあることを示している。 en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= en-aut-name=SatoYoshiro en-aut-sei=Sato en-aut-mei=Yoshiro kn-aut-name=佐藤美郎 kn-aut-sei=佐藤 kn-aut-mei=美郎 aut-affil-num=2 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=1 article-no= start-page=1 end-page=8 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=1991 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A Selective Accumulation of Some Leaf Xanthophylls in Hen Egg Yolk and Its Manner. kn-title=緑葉カロテノイドのニワトリ卵黄への選択的移行とその機構 en-subtitle= kn-subtitle= en-abstract= kn-abstract=ニワトリにおける緑葉カロテノイドの吸収と体組織分布について部分的に明らかにするとともに,とくに卵黄へのルテインの集中選択的蓄積に関する知見を得た. 投与した緑葉カロテノイド各々のみかけ吸収率は50～65%とカロテノイドによる差は認められないが,卵黄への蓄積量及び蓄積率はキサントフィルが高く,なかでもルテインはそのみかけ吸収量の25%が卵黄に蓄積している.これに対し,β－カロチンはみかけ吸収量のわずか0.6%しか蓄積せず,卵黄全カロテノイドに占める割合もルテインの約85%に対し1%である. 3日間の短期投与による緑葉カロテノイドの肝,卵巣,血液への正味蓄積量において,ルテインは肝よりも卵巣に6倍以上の速度で蓄積するのに対し,他のキサントフィル及びβ－カロテンは肝に多く蓄積する傾向がある.このことは緑葉カロテノイドのうちルテインがとくに卵黄に蓄積しやすいことを示すものである。 en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= en-aut-name=NakanoHiroyuki en-aut-sei=Nakano en-aut-mei=Hiroyuki kn-aut-name=中野浩行 kn-aut-sei=中野 kn-aut-mei=浩行 aut-affil-num=2 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=3 ORCID= en-aut-name=KondoYasuhiro en-aut-sei=Kondo en-aut-mei=Yasuhiro kn-aut-name=近藤康博 kn-aut-sei=近藤 kn-aut-mei=康博 aut-affil-num=4 ORCID= en-aut-name=TanabeAkira en-aut-sei=Tanabe en-aut-mei=Akira kn-aut-name=田辺昭 kn-aut-sei=田辺 kn-aut-mei=昭 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=緑葉カロテノイド kn-keyword=緑葉カロテノイド en-keyword=ニワトリ卵黄 kn-keyword=ニワトリ卵黄 en-keyword=ルテイン kn-keyword=ルテイン END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=1 article-no= start-page=1 end-page=7 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=1990 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=緑葉カロテノイドの季節的変動と環境因子 kn-title=A Seasonal Variation of Carotenoid Composition in Green Leaves and Effect of Environmental Factors on It+ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Carotenoid compositions of green leaves are varied with the passage of season. β-Carotene, one of the major carotenoid, is rich in summer and poor in spring, autumn and winter , while lutein, the other major, shows the opposite direction to β-carotene. These phenomena were confirmed to be always found in every plants, in both annual and perennial plants or in both herbaceous and woody plants. This variation was also confirmed to be seasonal and periodical on using green leaves of both evergreen and deciduous trees . The examinations for grapevine grown under different temperatures and light intensities revealed that these seasonal variations were mainly based on temperature. These results will provide important informations for both plant physiology and food nutrition. en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= en-aut-name=KishiFumiaki en-aut-sei=Kishi en-aut-mei=Fumiaki kn-aut-name=貴志文昭 kn-aut-sei=貴志 kn-aut-mei=文昭 aut-affil-num=2 ORCID= en-aut-name=NakajimaKentaro en-aut-sei=Nakajima en-aut-mei=Kentaro kn-aut-name=中島健太郎 kn-aut-sei=中島 kn-aut-mei=健太郎 aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=4 ORCID= en-aut-name=NakanoMikio en-aut-sei=Nakano en-aut-mei=Mikio kn-aut-name=中野幹夫 kn-aut-sei=中野 kn-aut-mei=幹夫 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 END