start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=2023217
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Preparation of crystalline polyimide nanofibers via solution crystallization
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Two crystalline polyimide nanofibers (PINFs) with different morphologies were prepared. The crystalline unit cells of the aromatic PI crystals and the crystal morphologies of the fabricated PINFs were examined. PINF-I (lengths?=?305?}?152?nm and diameters?=?12?}?2?nm) was crystallized from crystalline PI dissolved in a concentrated sulfuric acid solution. The resulting PINF-I was isolated from this solution, and it did not aggregate in water. PINF-II with diameters of 105?}?99?nm was prepared by dispersing PINF-I in a mixed water and t-butanol (TBA) solution (water:TBA?=?4:1), followed by freeze-drying. Then, the PINF-II was heated to enhance its crystallinity. X-ray diffraction and transmission electron microscopy studies of the heat-treated PINF-II revealed a PI crystalline unit cell [orthorhombic, a?=?1.21?nm, b?=?0.88?nm, and c?=?2.23?nm (molecular chain axis direction)]. The crystal structure of the heat-treated PINF-II suggested that highly crystalline PINFs were fabricated in which the PI molecular chains were oriented along the direction of the fiber lengths.
en-copyright=
kn-copyright=
en-aut-name=KumanoShota
en-aut-sei=Kumano
en-aut-mei=Shota
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TakakiTomoyasu
en-aut-sei=Takaki
en-aut-mei=Tomoyasu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=3
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
END
start-ver=1.4
cd-journal=joma
no-vol=33
cd-vols=
no-issue=8
article-no=
start-page=2511
end-page=2518
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220426
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Preparation of cellulose nanocrystals coated with polymer crystals and their application in composite films
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Increasing the dispersibility of cellulose nanocrystals (CNCs) as a reinforcing material is highly desirable to obtain composites with enhanced mechanical properties. To this aim, nanocomposite fibers (NCF(CNC/polymer)) were fabricated by coating CNCs with polymer crystals. Poly(vinyl alcohol) (PVA) and poly(vinyl alcohol-co-ethylene) (EVOH) were crystallized from a dilute solution in the presence of CNCs, which acted as nucleating agents for polymer crystal growth on the CNC surface. NCF consisting of CNCs coated with PVA crystals (NCF(CNC/PVA)) and CNCs coated with EVOH crystals (NCF(CNC/EVOH)) were successfully obtained. Both NCF(CNC/polymer) showed good dispersibility in water, even after drying, and were used to prepare composite films with improved mechanical properties. The reinforcement effect of NCF(CNC/PVA) was greater than that of pure CNCs and NCF(CNC/EVOH). The PVA composite films exhibited optical transmittance above 99% compared with additive-free PVA films. No agglomerates appeared even under the optical microscopic observation of the PVA composite film, and NCF(CNC/PVA) dispersibility was extremely good. NCF(CNC/PVA)-added PVA composite films exhibited increased glass transition temperature compared with additive-free PVA films, and the crystallinity of the PVA film increased. The highly dispersed CNCs in the PVA matrix and the increase in the glass transition temperature and crystallinity caused an increase in the mechanical modulus of the PVA composite film at low NCF(CNC/PVA) content. Furthermore, the effect of the morphologies of cellulose nanofibers and CNCs on the mechanical properties of the composite films prepared using the respective NCFs was investigated.
en-copyright=
kn-copyright=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=NishiokaRyohei
en-aut-sei=Nishioka
en-aut-mei=Ryohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=YanaiRisa
en-aut-sei=Yanai
en-aut-mei=Risa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=3
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=cellulose nanocrystals
kn-keyword=cellulose nanocrystals
en-keyword=composite
kn-keyword=composite
en-keyword=dispersibility
kn-keyword=dispersibility
en-keyword=mechanical properties
kn-keyword=mechanical properties
en-keyword=surface treatment
kn-keyword=surface treatment
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=1
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202199
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Preparation of highly porous heat]resistant polybenzoxazole network films and their electrical conductivities
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Highly porous rigid polybenzoxazole (PBO) network films were prepared using a precursor-mediated fabrication method. The obtained PBO network films possessed high porosities of similar to 40%, as calculated from their apparent densities. In addition, the 5%-weight-loss temperatures of the films were >= 570 degrees C under nitrogen atmosphere, demonstrating an excellent thermal stability. The electrical conductivities of the obtained PBO network films and phosphoric-acid-doped PBO network films were also evaluated. In addition, PBO network films containing pyridine rings were prepared and subjected to phosphoric acid doping. The resultant films were found to exhibit the highest conductivities of the films considered in this study owing to proton conduction both between phosphate groups and between the pyridine rings. The highest conductivity was found for a film prepared from a phosphoric-acid-doped PBO network containing pyridine rings, that is, 2.09 x 10(-1) S/cm at 150 degrees C, which was higher than that of Nafion (TM). Therefore, these films can be used at higher temperatures than that of Nafion (TM).
en-copyright=
kn-copyright=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=OnishiYukihisa
en-aut-sei=Onishi
en-aut-mei=Yukihisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=GotoAtsu
en-aut-sei=Goto
en-aut-mei=Atsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=Graduate School of Natural Science and Technology Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Natural Science and Technology Okayama University
kn-affil=
affil-num=3
en-affil=Graduate School of Natural Science and Technology Okayama University
kn-affil=
en-keyword=rigid polymer network
kn-keyword=rigid polymer network
en-keyword=heat resistance
kn-keyword=heat resistance
en-keyword=proton
kn-keyword=proton
en-keyword=phosphoric acid
kn-keyword=phosphoric acid
en-keyword=conductivity
kn-keyword=conductivity
END
start-ver=1.4
cd-journal=joma
no-vol=11
cd-vols=
no-issue=6
article-no=
start-page=591
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=2021621
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Photoelectric Dye, NK-5962, as a Potential Drug for Preventing Retinal Neurons from Apoptosis: Pharmacokinetic Studies Based on Review of the Evidence
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=NK-5962 is a key component of photoelectric dye-based retinal prosthesis (OUReP). In testing the safety and efficacy, NK-5962 was safe in all tests for the biological evaluation of medical devices (ISO 10993) and effective in preventing retinal cells from death even under dark conditions. The long-term implantation of the photoelectric dye-coupled polyethylene film in the subretinal space of hereditary retinal dystrophic (RCS) rats prevented neurons from apoptosis in the adjacent retinal tissue. The intravitreous injection of NK-5962 in the eyes of RCS rats, indeed, reduced the number of apoptotic cells in the retinal outer nuclear layer irrespective of light or dark conditions. In this study, we reviewed the in vitro and in vivo evidence of neuroprotective effect of NK-5962 and designed pharmacokinetic experiments. The in vitro IC50 of 1.7 ƒÊM, based on the protective effect on retinal cells in culture, could explain the in vivo EC50 of 3 ƒÊM that is calculated from concentrations of intravitreous injection to prevent retinal neurons from apoptosis. Pharmacokinetics of NK-5962 showed that intravenous administration, but not oral administration, led to the effective concentration in the eye of rats. NK-5962 would be a candidate drug for delaying the deterioration of retinal dystrophy, such as retinitis pigmentosa.
en-copyright=
kn-copyright=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=LiuShihui
en-aut-sei=Liu
en-aut-mei=Shihui
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=OnoueSatomi
en-aut-sei=Onoue
en-aut-mei=Satomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=NakagawaShinsaku
en-aut-sei=Nakagawa
en-aut-mei=Shinsaku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=IshiiMayumi
en-aut-sei=Ishii
en-aut-mei=Mayumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=KanamitsuKayoko
en-aut-sei=Kanamitsu
en-aut-mei=Kayoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
affil-num=1
en-affil=Department of Ophthalmology, Okayama University Hospital
kn-affil=
affil-num=2
en-affil=Okayama University Graduate School of Interdisciplinary Science and Engineering in Health Systems
kn-affil=
affil-num=3
en-affil=Polymer Materials Science, Okayama University Graduate School of Natural Science and Technology
kn-affil=
affil-num=4
en-affil=Laboratory of Biopharmacy, School of Pharmaceutical Sciences, University of Shizuoka
kn-affil=
affil-num=5
en-affil=Laboratory of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University
kn-affil=
affil-num=6
en-affil=Drug Discovery Initiative, The University of Tokyo
kn-affil=
affil-num=7
en-affil=Drug Discovery Initiative, The University of Tokyo
kn-affil=
en-keyword=NK-5962
kn-keyword=NK-5962
en-keyword=photoelectric dye
kn-keyword=photoelectric dye
en-keyword=apoptosis
kn-keyword=apoptosis
en-keyword=retinal neuron
kn-keyword=retinal neuron
en-keyword=neuroprotection
kn-keyword=neuroprotection
en-keyword=pharmacokinetics
kn-keyword=pharmacokinetics
en-keyword=ADME
kn-keyword=ADME
en-keyword=phototoxic/photosensitive assay
kn-keyword=phototoxic/photosensitive assay
en-keyword=reactive oxygen species assay
kn-keyword=reactive oxygen species assay
en-keyword=photosafety
kn-keyword=photosafety
END
start-ver=1.4
cd-journal=joma
no-vol=18
cd-vols=
no-issue=4
article-no=
start-page=045006
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202155
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Modelling the visual response to an OUReP retinal prosthesis with photoelectric dye coupled to polyethylene film
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Objective. Retinal prostheses have been developed to restore vision in blind patients suffering from diseases like retinitis pigmentosa. Approach. A new type of retinal prosthesis called the Okayama University-type retinal prosthesis (OUReP) was developed by chemically coupling photoelectric dyes to a polyethylene film surface. The prosthesis works by passively generating an electric potential when stimulated by light. However, the neurophysiological mechanism of how OUReP stimulates the degenerated retina is unknown. Main results. Here, we explore how the OUReP affects retinal tissues using a finite element model to solve for the potential inside the tissue and an active Hodgkin-Huxley model based on rat vision to predict the corresponding retinal bipolar response. Significance. We show that the OUReP is likely capable of eliciting responses in retinal bipolar cells necessary to generate vision under most ambient conditions.
en-copyright=
kn-copyright=
en-aut-name=YamashitaKoichiro
en-aut-sei=Yamashita
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=SundaramPrathima
en-aut-sei=Sundaram
en-aut-mei=Prathima
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=WongWilly
en-aut-sei=Wong
en-aut-mei=Willy
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Electrical and Computer Engineering, University of Toronto
kn-affil=
affil-num=3
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=4
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=5
en-affil=Department of Electrical and Computer Engineering, University of Toronto
kn-affil=
en-keyword=retinal prosthesis
kn-keyword=retinal prosthesis
en-keyword=photoelectric dye
kn-keyword=photoelectric dye
en-keyword=boundary value problem
kn-keyword=boundary value problem
en-keyword=biophysical models of retinal cells
kn-keyword=biophysical models of retinal cells
END
start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=4
article-no=
start-page=045001
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202138
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Development of highly durable retinal prosthesis using photoelectric dyes coupled to polyethylene film and quantitative in vitro evaluation of its durability
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Retinal prostheses have been developed to restore vision in blind patients suffering from such diseases as retinitis pigmentosa. In our previous studies, we developed a retinal prosthesis called dye-coupled film by chemical coupling of photoelectric dyes, which absorb light and then generate electrical potential, with a polyethylene film surface. The dye-coupled film is nontoxic, and we recovered the vision of a monkey with macular degeneration. The amount of dye on the dye-coupled film, however, decreased to one-third after five months in the monkeyfs eye. The photoelectric dye consists of a cation with photoresponsivity and a bromide ion (Br?). Therefore, an anion-exchange reaction could be applied to the dye-coupled film to improve its durability. In this study, the anion-exchange reaction was conducted using bis(trifluoromethanesulfonyl)imide ion (TFSI?), which has lower nucleophilicity than Br?. First, the long-term durability was examined without using animal subjects and in a short period. Subsequently, an elemental analysis was performed to confirm the exchange between Br? and TFSI?, and chemical properties, such as photoresponsivity and durability, before and after the anion exchange, were evaluated. It was quantitatively confirmed that the long-term durability of dye-coupled films can be evaluated in an in vitro environment and in a short period of one-thirtieth by utilizing a saline solution at 60‹C, compared with an in vivo environment. In addition, the durability of the dye-coupled film with TFSI? was improved to 270%?320% compared with that of the dye-coupled film with Br?.
en-copyright=
kn-copyright=
en-aut-name=YamashitaKoichiro
en-aut-sei=Yamashita
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TanakaTenu
en-aut-sei=Tanaka
en-aut-mei=Tenu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=3
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems
kn-affil=
affil-num=4
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=retinal prosthesis
kn-keyword=retinal prosthesis
en-keyword=photoelectric dye
kn-keyword=photoelectric dye
en-keyword=polyethylene film
kn-keyword=polyethylene film
en-keyword=retinitis pigmentosa
kn-keyword=retinitis pigmentosa
en-keyword=durability
kn-keyword=durability
END
start-ver=1.4
cd-journal=joma
no-vol=307
cd-vols=
no-issue=
article-no=
start-page=130978
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20211001
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Clarification of degradation mechanism on retinal prosthesis using photoelectric dyes coupled to polyethylene film by mass spectrometry
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Photoelectric dyes have stimulated retinal neurons by absorbing light and generating an electric potential. Therefore, a photoelectric dye was used to develop a retinal prosthesis to restore vision loss due to retinitis pigmentosa. The retinal prosthesis, referred to as a dye-coupled film, was prepared by chemically coupling the dyes to a polyethylene film surface through amide bonds. However, the coupled dyes decreased during an implantation test in a monkey eye. This study clarifies the degradation mechanism of the dye-coupled film. Since the dyes were selectively coupled to the film surface, it is difficult to detect the eliminated dyes in a solution. Therefore, a model compound that mimicked the chemical structure of the dye-coupled film by converting the carboxylic acid of dye to the amide bond is used. It was found that the elimination of the side chain, including the amide bond, occurred before the degradation of the conjugated structure according to mass spectrometry of the model compound. The degradation mechanism of the dye-coupled film was predicted based on that of the model compound. It was concluded that chemical bonds between the dye and the film surface were preferentially decomposed, leading to the detachment of the dyes from the film surface.
en-copyright=
kn-copyright=
en-aut-name=YamashitaKoichiro
en-aut-sei=Yamashita
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MitsuiMayu
en-aut-sei=Mitsui
en-aut-mei=Mayu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=3
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=4
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=Degradation
kn-keyword=Degradation
en-keyword=Photoelectric dye
kn-keyword=Photoelectric dye
en-keyword=Benzothiazole
kn-keyword=Benzothiazole
en-keyword=Mass spectrometry
kn-keyword=Mass spectrometry
en-keyword=Retinal prosthesis
kn-keyword=Retinal prosthesis
END
start-ver=1.4
cd-journal=joma
no-vol=7
cd-vols=
no-issue=1
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=2021
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Photoelectric dye-based retinal prosthesis (OUReP) as a novel type of artificial retina
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We have developed the world's first novel type of artificial retina, OUReP (Okayama University Retinal Prosthesis), in which a photoelectric dye that converts light energy into electric potential is covalently bonded to the surface of a polyethylene thin film as an insulator. The receptor that absorbs light and the output device that generates displacement current to stimulate nearby neurons are integrated in a sheet of thin film. It has become possible to measure the surface potential of the artificial retina OUReP using a Kelvin probe that measures the surface potential of semiconductors. When light is turned on and off to the artificial retina OUReP, the surface potential changes rapidly. As the light intensity is increased, the potential change on the surface of the artificial retina becomes larger. As for safety, the artificial retina OUReP was not toxic in all tests for biological evaluation of medical devices. As for efficacy, the artificial retina OUReP was implanted under the retina by vitreous surgery in monkey eyes which had chemically-induced macular degeneration with photoreceptor cell loss. Over the next 6 months, retinal detachment did not occur during the course, and the artificial retina was in contact with the retinal tissue. The amplitude of the visual evoked potential attenuated by macular degeneration recovered 1 month after implantation of the artificial retina, and the recovery of amplitude was maintained until 6 months after the implantation. By using multielectrode array-mounted dish recording system, it has been proved that action potential spikes are induced when the artificial retina is placed on degenerative retinal tissue of retinal dystrophic rats or mice and exposed to light, which is used as an index of the effectiveness of the artificial retina. We have established manufacturing and quality control of the device in a clean room facility, proved the safety and efficacy, and are preparing for first-in-human investigator-initiated clinical trials.
en-copyright=
kn-copyright=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
affil-num=1
en-affil=Ophthalmology, Okayama University Hospital
kn-affil=
affil-num=2
en-affil=olymer Materials Science, Okayama University Graduate School of Natural Science and Technology,
kn-affil=
en-keyword=artificial retina
kn-keyword=artificial retina
en-keyword=retinal prosthesis
kn-keyword=retinal prosthesis
en-keyword=photoelectric dye
kn-keyword=photoelectric dye
en-keyword=polyethylene film
kn-keyword=polyethylene film
en-keyword=monkey surgery
kn-keyword=monkey surgery
en-keyword=multielectrode array dish recording
kn-keyword=multielectrode array dish recording
en-keyword=vitrectomy
kn-keyword=vitrectomy
en-keyword=disposable injector
kn-keyword=disposable injector
en-keyword=sustainable development goals
kn-keyword=sustainable development goals
END
start-ver=1.4
cd-journal=joma
no-vol=4
cd-vols=
no-issue=3
article-no=
start-page=1040
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200923
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Curved-Tip Disposable Injector (OUReP Injector) to Insert Photoelectric Dye-Coupled Polyethylene Film (OUReP) as Retinal Prosthesis into Subretinal Space of Rabbit Eyes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The photoelectric dye-coupled thin polyethylene film functions as a novel type of retinal prosthesis in subretinal space of the eye. We previously reported a novel disposable injector to insert the thin film into subretinal space of the rabbit eye by vitreous surgery. The injection system composed of two separate parts, injector and loader. A circular film in 5 mm to 10 mm diameter was pulled into a transparent tube of the loader with a commercial 25-gauge forceps. The loader tube was joined with a sleeve to tube tip of the injector. The film in the loader was pushed with a plunger for the loader into the injector tube tip. The loader with the sleeve was removed from the injector tip, and the tube tip with the film was filled with solution. This study reported a small-bore curved-tip disposable injector with outer diameter of 1.6 mm. A curved-tip polypropylene tube was formed by the process of heating and cooling of the tube inserted with a curved guide wire. The plunger for the curved-tip tube was made of a polyphenylsulfone tip connected with a press-fitting inner polypropylene tube to a nylon rod. Bleb retinal detachment in 4 surgically aphakic eyes of rabbits were induced by infusing solution into subretinal space with a 38-gauge polyimide tip, and a retinal tear was made at the edge of retinal detachment with 25-gauge diathermy. The injector tip with the rolled film in 6 mm diameter was inserted from 2 mm wide scleral incision into vitreous and then into subretinal space. The rolled films were released into subretinal space with the tip end inserted into the retinal tear, and the released films were confirmed to stay under the retina with no additional aid. Dissection one month after surgeries confirmed successful implantation of 4 films into subretinal space of each rabbit eye. The curved-tip injector could release the rolled film into the subretinal space without additional effort, compared with the straight-tip injector, and would help surgeons implant photoelectric dye-coupled thin film retinal prosthesis easily at vitreous surgery.
en-copyright=
kn-copyright=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=matsuoChie
en-aut-sei=matsuo
en-aut-mei=Chie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=YamashitaKoichiro
en-aut-sei=Yamashita
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=TanakaTenu
en-aut-sei=Tanaka
en-aut-mei=Tenu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=KawakamiYusuke
en-aut-sei=Kawakami
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=HitomiToshiya
en-aut-sei=Hitomi
en-aut-mei=Toshiya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=TagaKanji
en-aut-sei=Taga
en-aut-mei=Kanji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=SanadaTatsuya
en-aut-sei=Sanada
en-aut-mei=Tatsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=YamashitaYusuke
en-aut-sei=Yamashita
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Department of Regenerative and Reconstructive Medicine (Ophthalmology), Okayama University Graduate School of Interdisciplinary Science and Engineering in Health Systems
kn-affil=
affil-num=2
en-affil=Department of Regenerative and Reconstructive Medicine (Ophthalmology), Okayama University Graduate School of Interdisciplinary Science and Engineering in Health Systems
kn-affil=
affil-num=3
en-affil=Department of Polymer Materials Science, Okayama University Faculty of Engineering and Graduate School of Natural Science and Technology
kn-affil=
affil-num=4
en-affil=Department of Polymer Materials Science, Okayama University Faculty of Engineering and Graduate School of Natural Science and Technology
kn-affil=
affil-num=5
en-affil=Department of Polymer Materials Science, Okayama University Faculty of Engineering and Graduate School of Natural Science and Technology
kn-affil=
affil-num=6
en-affil=Minori Industry Co. Ltd.
kn-affil=
affil-num=7
en-affil=Minori Industry Co. Ltd.
kn-affil=
affil-num=8
en-affil=Minori Industry Co. Ltd.
kn-affil=
affil-num=9
en-affil=Minori Industry Co. Ltd.
kn-affil=
affil-num=10
en-affil=Shin Nippon Biomedical Laboratories Ltd.
kn-affil=
en-keyword=Dye-coupled thin film retinal prosthesis
kn-keyword=Dye-coupled thin film retinal prosthesis
en-keyword=Curved-tip injector
kn-keyword=Curved-tip injector
en-keyword=Photoelectric dye
kn-keyword=Photoelectric dye
en-keyword=Rabbit
kn-keyword=Rabbit
en-keyword=25G vitrectomy
kn-keyword=25G vitrectomy
END
start-ver=1.4
cd-journal=joma
no-vol=202
cd-vols=
no-issue=
article-no=
start-page=122672
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200812
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Preparation of solution-grown lozenge-shaped poly(p-phenylene terephthalamide) single crystals and their structural stabilization by heat treatment
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In this study, the preparation of poly (p-phenylene terephthalamide) (PPTA) single crystals was examined using crystallization from dilute solutions in concentrated sulfuric acid. Lozenge-shaped PPTA single crystals were successfully prepared using a self-seeding method with a low degree of supercooling, and they consisted of modification I crystals. The a-axis direction of the crystal corresponded to the long diagonal direction of the rhombus, the b-axis direction with the short diagonal direction, and the PPTA molecular chain direction (the c-axis direction) with the crystal's thickness direction. In addition, the PPTA single crystals had a (110) growth plane, where the thickness of each single crystal was approximately equal to the molecular chain length of the PPTA. Upon heat treatment of the PPTA single crystals, the symmetry changed from P1a1 to the more stable P11n. In addition, the heat treatment caused a difference in the density of each symmetric crystal, resulting in crack formation along the b-axis direction, which is the hydrogen-bonding direction. However, the heat treatment did not change the thickness of the PPTA single crystals. Conversely, the isothermal crystallization of the PPTA caused progression in the crystallization only under a high degree of supercooling, thus yielding plate-like PPTA crystals that consisted of modification II crystals. In these plate-like PPTA crystals, the length corresponded to the crystal a-axis direction, and the electron diffraction pattern was broad. Furthermore, the equilibrium dissolution temperature of the PPTA single crystals was discussed.
en-copyright=
kn-copyright=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HaraYutaro
en-aut-sei=Hara
en-aut-mei=Yutaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TakakiTomoyasu
en-aut-sei=Takaki
en-aut-mei=Tomoyasu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=3
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=Poly(p-phenylene terephthalamide)
kn-keyword=Poly(p-phenylene terephthalamide)
en-keyword=Single crystals
kn-keyword=Single crystals
en-keyword=Heat treatment
kn-keyword=Heat treatment
END
start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=
article-no=
start-page=2903
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Step-by-Step Procedure to Test Photoelectric Dye-Coupled Polyethylene Film as Retinal Prosthesis to Induce Light-Evoked Spikes in Isolated Retinal Dystrophic Tissue of rd1 Mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Purpose: Multielectrode array recording for electric activity in cardiac and neuronal cells has been developed as preclinical tests for drug screening. This study aims to establish an in vitro assay system, using the multielectrode array, to record light-evoked spikes in isolated degenerative retinal tissues of retinal dystrophic rd1 mouse, as a preclinical test to examine the efficacy of photoelectric
dye-coupled thin film retinal prosthesis.
Methods: Light-evoked spike response was tested for 1 min at first step in the isolated degenerative retinal tissue of retinal dystrophic rd1 mouse only on the multielectrode array, tested in the same retinal tissue overlain with a plain control film for light-off and light-on 10 min each at second step, and tested in the same tissue overlain with a dye-coupled film at third step. The retinal tissues which showed light-evoked response at first or second step were not used for evaluation at third step.
Results: Residual light-evoked spikes were recorded at first or second step in 18 of 35 retinal tissues (51%) at 6 weeks of the age in rd1 mice, 16 of 44 tissues (36%) at 7 weeks, and 10 of 39 tissues (25%) at 8 weeks. At third step, light-evoked spikes were recorded with dye-coupled films in 8 of 17 retinal tissues (47%) at 6 weeks, 10 of 28 tissues (35%) at 7 weeks, and 8 of 29 tissues (27%) at 8 weeks.
Conclusion: A step-by-step procedure with internal control was established to measure light-evoked spikes by the multielectrode array in the isolated degenerative retinal tissue to evaluate photoelectric dye-coupled thin films. This preclinical study would present one line of evidence for the efficacy of photoelectric dye-coupled thin film retinal prosthesis towards a first-in-human clinical trial.
en-copyright=
kn-copyright=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TeradaKeiko
en-aut-sei=Terada
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=SakuraiMikako
en-aut-sei=Sakurai
en-aut-mei=Mikako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=LiuShihu
en-aut-sei=Liu
en-aut-mei=Shihu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=YamashitaKoichiro
en-aut-sei=Yamashita
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
affil-num=1
en-affil=Regenerative and Reconstructive Medicine (Ophthalmology), Okayama University Medical School and Graduate School of Interdisciplinary Science and Engineering in Health Systems
kn-affil=
affil-num=2
en-affil=Research Center, Techno Pro, Inc.
kn-affil=
affil-num=3
en-affil=Research Center, Techno Pro, Inc.
kn-affil=
affil-num=4
en-affil=Regenerative and Reconstructive Medicine (Ophthalmology), Okayama University Medical School and Graduate School of Interdisciplinary Science and Engineering in Health Systems
kn-affil=
affil-num=5
en-affil=Polymer Materials Science, Okayama University Faculty of Engineering and Graduate School of Natural Science and Technology
kn-affil=
affil-num=6
en-affil=Polymer Materials Science, Okayama University Faculty of Engineering and Graduate School of Natural Science and Technology
kn-affil=
en-keyword=Photoelectric dye
kn-keyword=Photoelectric dye
en-keyword=Polyethylene thin film
kn-keyword=Polyethylene thin film
en-keyword=Spike
kn-keyword=Spike
en-keyword=Degenerative retina
kn-keyword=Degenerative retina
en-keyword=Retinal dystrophic rd1 mouse
kn-keyword=Retinal dystrophic rd1 mouse
END
start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=6
article-no=
start-page=e01936
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Vision evaluation by functional observational battery, operant behavior test, and light/dark box test in retinal dystrophic RCS rats versus normal rats
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=BACKGROUND:
Vision plays a key role in some behavior tests for rats. Okayama University-type retinal prosthesis (OUReP) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to assess vision in retinal dystrophic (RCS) rats, in comparison with normal rats, by selected behavior tests. We also examined whether the tests could detect vision changes in RCS rats with dye-coupled film implantation.
METHODS:
Data sets were 5 normal rats, 4 untreated RCS rats, 7 RCS rats with dye-coupled films implanted at the age of 7 weeks after excluding unsuccessful implantation at autopsy. Behavior tests chosen were landing foot splay and visual forelimb-placing response in the menu of functional observational battery, operant-conditioning lever-press response and light/dark box test.
RESULTS:
Normal visual placing response was significantly less frequent in untreated RCS rats at the age of 9 and 11 weeks, compared with normal rats (P = 0.0027, chi-square test) while normal response was significantly more frequent at the age of 9 weeks in RCS rats with dye-coupled film implantation, compared with untreated RCS rats (P = 0.0221). In operant-conditioning lever-press test, the correct response rate was significantly lower in untreated RCS rats than in normal rats at the age of 9 weeks (P < 0.05, Tukey-Kramer test) while the rate was not significantly different between normal rats and RCS rats with dye-coupled film implantation. In light/dark box test, the time to enter dark box was significantly shorter in normal rats, compared with untreated RCS rats or RCS rats with dye-coupled film implantation (P < 0.05, Tukey-Kramer test).
CONCLUSIONS:
Behavior tests of functional observational battery, operant-conditioning lever-press response and light/dark box test discriminated vision between normal rats and RCS rats. The visual placing response and operant-conditioning lever-press test might have sensitivity to detect vision recovery in RCS rats with OUReP implantation.
en-copyright=
kn-copyright=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=YamashitaKoichiro
en-aut-sei=Yamashita
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=Takei Shigiko
en-aut-sei=Takei
en-aut-mei= Shigiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=Ido Daisuke
en-aut-sei=Ido
en-aut-mei= Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=Fujiwara Atsushi
en-aut-sei=Fujiwara
en-aut-mei= Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=Iino Masahiko
en-aut-sei=Iino
en-aut-mei= Masahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=Oguchi Masao
en-aut-sei=Oguchi
en-aut-mei= Masao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=Ophthalmology, Okayama University Medical School and Okayama University Hospital
kn-affil=
affil-num=2
en-affil=Polymer Materials Science, Okayama University Graduate School of Natural Science and Technology
kn-affil=
affil-num=3
en-affil=Polymer Materials Science, Okayama University Graduate School of Natural Science and Technology
kn-affil=
affil-num=4
en-affil=Ina Research
kn-affil=
affil-num=5
en-affil=Ina Research
kn-affil=
affil-num=6
en-affil=Ina Research
kn-affil=
affil-num=7
en-affil=Ina Research
kn-affil=
affil-num=8
en-affil=Ina Research
kn-affil=
en-keyword=Bioengineering
kn-keyword=Bioengineering
en-keyword=Neuroscience
kn-keyword=Neuroscience
en-keyword=Physiology
kn-keyword=Physiology
en-keyword=Zoology
kn-keyword=Zoology
END
start-ver=1.4
cd-journal=joma
no-vol=127
cd-vols=
no-issue=3
article-no=
start-page=223
end-page=229
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20151201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Investigator (doctor)-initiated clinical trial of photoelectric dye-coupled thin film (Okayama University-type) retinal prosthesis
kn-title=F‘fŒ‹‡”––ŒŒ^i‰ªŽR‘åŠw•ûŽ®j‚ÌlH–Ô–ŒiOURePTMj‚̈ãŽtŽå“±Ž¡Œ±
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=¼”ör•F
kn-aut-sei=¼”ö
kn-aut-mei=r•F
aut-affil-num=1
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=“à“c“N–ç
kn-aut-sei=“à“c
kn-aut-mei=“N–ç
aut-affil-num=2
ORCID=
affil-num=1
en-affil=
kn-affil=‰ªŽR‘åŠw‘åŠw‰@ˆãŽ•–òŠw‘‡Œ¤‹†‰È
affil-num=2
en-affil=
kn-affil=‰ªŽR‘åŠw‘åŠw‰@Ž©‘R‰ÈŠwŒ¤‹†‰È
en-keyword=lH–Ô–Œ
kn-keyword=lH–Ô–Œ
en-keyword=OUReP
kn-keyword=OUReP
en-keyword=ˆãŽtŽå“±Ž¡Œ±
kn-keyword=ˆãŽtŽå“±Ž¡Œ±
en-keyword=F‘fŒ‹‡”––Œ
kn-keyword=F‘fŒ‹‡”––Œ
en-keyword=–Ô–ŒF‘f•Ï«
kn-keyword=–Ô–ŒF‘f•Ï«
END
start-ver=1.4
cd-journal=joma
no-vol=3
cd-vols=
no-issue=
article-no=
start-page=317
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140625
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Glass transition temperature of dried lens tissue pretreated with trehalose, maltose, or cyclic tetrasaccharide
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=[PURPOSE]
Glass transition temperature is a main indicator for amorphous polymers and biological macromolecules as materials, and would be a key for understanding the role of trehalose in protecting proteins and cells against desiccation. In this study, we measured the glass transition temperature by differential scanning calorimetry of dried lens tissues as a model of a whole biological tissue to know the effect of pretreatment by trehalose and other sugars.
[METHODS]
Isolated porcine lenses were incubated with saline, 100 or 1000 mM concentration of trehalose, maltose, or cyclic tetrasaccharide dissolved in saline at room temperature for 150 minutes. The solutions were removed and all samples were dried at room temperature in a desiccator until no weight change. The dried tissues were ground into powder and placed in a measuring pan for differential scanning calorimetry.
[RESULTS]
The glass transition temperature of the dried lens tissues, as a mean and standard deviation, was 63.0?}?6.4‹C (n?=?3) with saline pretreatment; 53.0?}?0.8‹C and 56.3?}?2.7‹C (n?=?3), respectively, with 100 and 1000 mM trehalose pretreatment; 56.0?}?1.6‹C and 55.8?}?1.1‹C (n?=?3), respectively, with 100 and 1000 mM maltose pretreatment; 60.0?}?8.8‹C and 59.2?}?6.3‹C (n?=?3), respectively, with 100 and 1000 mM cyclic tetrasaccharide pretreatment. The glass transition temperature appeared lower, although not significantly, with trehalose and maltose pretreatments than with saline and cyclic tetrasaccharide pretreatments (P?>?0.05, Kruskal-Wallis test). The glass transition temperature of the dried lens tissues with trehalose pretreatment appeared more noticeable on the thermogram, compared with other pretreatments.
[CONCLUSIONS]
The glass transition temperature was measured for the first time in the dried lens tissues as an example of a whole biological tissue and might provide a basis for tissue preservation in the dried condition.
en-copyright=
kn-copyright=
en-aut-name=KawataTetsuhiro
en-aut-sei=Kawata
en-aut-mei=Tetsuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Medical School and Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
affil-num=2
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Medical School and Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
affil-num=3
en-affil=
kn-affil=Division of Polymer Materials Science, Faculty of Engineering and Okayama University Graduate School of Natural Science and Technology
en-keyword=Trehalose
kn-keyword=Trehalose
en-keyword=Glass transition temperature
kn-keyword=Glass transition temperature
en-keyword=Lens
kn-keyword=Lens
en-keyword=Differential scanning calorimetry
kn-keyword=Differential scanning calorimetry
en-keyword=Desiccation; Maltose
kn-keyword=Desiccation; Maltose
en-keyword=Cyclic tetrasaccharide
kn-keyword=Cyclic tetrasaccharide
en-keyword=Biological tissue
kn-keyword=Biological tissue
en-keyword=Tissue preservation
kn-keyword=Tissue preservation
en-keyword=Regenerative medicine
kn-keyword=Regenerative medicine
END
start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=3
article-no=
start-page=343
end-page=351
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201309
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Behavior tests and immunohistochemical retinal response analyses in RCS rats with subretinal implantation of Okayama-University-type retinal prosthesis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We have developed a photoelectric dye-coupled polyethylene film as a prototype of retinal prosthesis, which we named Okayama University-type retinal prosthesis. The purposes of this study are to conduct behavior tests to assess vision in Royal College of Surgeons (RCS) rats that underwent subretinal implantation of the dye-coupled film and to reveal retinal response to the dye-coupled film by immunohistochemistry. Polyethylene films were made of polyethylene powder at refined purity, and photoelectric dyes were coupled to the film surface at higher density compared with the prototype. Either dye-coupled film or dye-uncoupled plain film used as a control was implanted subretinally from a scleral incision in both eyes of an RCS rat at 6 weeks of the age. Behavior tests 2, 4, 6, and 8 weeks after implantation were conducted by observing head turning or body turning in the direction consistent with clockwise or counterclockwise rotation of a black-and-white-striped drum around a transparent cage housed with the rat. After the behavior tests at 8 weeks, rats' eyes were enucleated to confirm subretinal implantation of the films and processed for immunohistochemistry. In the behavior tests, the number of head turnings consistent with the direction of the drum rotation was significantly larger in RCS rats with dye-coupled- compared with plain-film implantation [P < 0.05, repeated-measure analysis of variance (ANOVA), n = 7]. The number of apoptotic neurons was significantly smaller in eyes with dye-coupled- compared with plain-film implantation (P < 0.05, Mann-Whitney U test, n = 6). In conclusion, subretinal implantation of photoelectric dye-coupled films restored vision in RCS rats and prevented the remaining retinal neurons from apoptosis.
en-copyright=
kn-copyright=
en-aut-name=Alamusi
en-aut-sei=Alamusi
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=HosoyaOsamu
en-aut-sei=Hosoya
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=TsutsuiKimiko M.
en-aut-sei=Tsutsui
en-aut-mei=Kimiko M.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=
kn-affil=Okayama Univ Med Sch, Dept Ophthalmol
affil-num=2
en-affil=
kn-affil=Okayama Univ Med Sch, Dept Ophthalmol
affil-num=3
en-affil=
kn-affil=Okayama Univ Med Sch, Dept Neurogen
affil-num=4
en-affil=
kn-affil=Okayama Univ Med Sch, Dept Neurogen
affil-num=5
en-affil=
kn-affil=Okayama Univ Fac Engn, Div Polymer Mat Sci
en-keyword=Retinal prosthesis
kn-keyword=Retinal prosthesis
en-keyword=Photoelectric dye
kn-keyword=Photoelectric dye
en-keyword=Polyethylene film
kn-keyword=Polyethylene film
en-keyword=RCS rat
kn-keyword=RCS rat
en-keyword=Behavior test
kn-keyword=Behavior test
en-keyword=Apoptosis
kn-keyword=Apoptosis
en-keyword=Immunohistochemistry
kn-keyword=Immunohistochemistry
END
start-ver=1.4
cd-journal=joma
no-vol=39
cd-vols=
no-issue=1
article-no=
start-page=16
end-page=20
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2005
dt-pub=200501
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Immobilization of Photoelectric Dye on the Polyethylene FilmSurface
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=PE film was treated with fuming nitric acid at 80Ž for 20 min, resulting in introduction of COOH moieties on the film surface. The COOHfs were reacted with ethylenediamine,
whose amino groups were used for linking with (2-[4-(dibutylamino)phenyl]ethenyl)-3-carboxy-methylbenzo-thiazolium, photoelectric dye (NK-5962), which absorbs visible light and converts the photon energy to electric potentials. The dye molecules were immobilized
on the PE film surface and they were able to stimulate chick retinal tissues on incidence of visible light. These facts hopefully lead to development of an artificial retinal prosthesis.
en-copyright=
kn-copyright=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=“à“c“N–ç
kn-aut-sei=“à“c
kn-aut-mei=“N–ç
aut-affil-num=1
ORCID=
en-aut-name=IshimaruSanae
en-aut-sei=Ishimaru
en-aut-mei=Sanae
kn-aut-name=Ίۑ•c
kn-aut-sei=ΊÛ
kn-aut-mei=‘•c
aut-affil-num=2
ORCID=
en-aut-name=ShimamuraKaoru
en-aut-sei=Shimamura
en-aut-mei=Kaoru
kn-aut-name=“‡‘ºŒO
kn-aut-sei=“‡‘º
kn-aut-mei=ŒO
aut-affil-num=3
ORCID=
en-aut-name=UjiAkihito
en-aut-sei=Uji
en-aut-mei=Akihito
kn-aut-name=‰FŽ¡Íl
kn-aut-sei=‰FŽ¡
kn-aut-mei=Íl
aut-affil-num=4
ORCID=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=¼”ör•F
kn-aut-sei=¼”ö
kn-aut-mei=r•F
aut-affil-num=5
ORCID=
en-aut-name=OhtsukiHiroshi
en-aut-sei=Ohtsuki
en-aut-mei=Hiroshi
kn-aut-name=‘匎—m
kn-aut-sei=‘匎
kn-aut-mei=—m
aut-affil-num=6
ORCID=
affil-num=1
en-affil=
kn-affil=
affil-num=2
en-affil=
kn-affil=
affil-num=3
en-affil=
kn-affil=
affil-num=4
en-affil=
kn-affil=
affil-num=5
en-affil=
kn-affil=
affil-num=6
en-affil=
kn-affil=
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=20020930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=„’¼‚•ªŽq‚ÌŒ‹»‚¨‚æ‚щˋ´‘Ì‚Ì\‘¢‚ÉŠÖ‚·‚錤‹†
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=“à“c“N–ç
kn-aut-sei=“à“c
kn-aut-mei=“N–ç
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=‰ªŽR‘åŠw
END
start-ver=1.4
cd-journal=joma
no-vol=35
cd-vols=
no-issue=1-2
article-no=
start-page=29
end-page=34
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2001
dt-pub=20010327
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Synthesis of Poly(ethylene-block-vinylalcohol)for Use as Amphiphilic Film Surface at High Temperature
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Poly (ethylene-block-vinyl alcohol), which consisted of both hydrophobic and hydrophilic blocks, was prepared by using polyethylene single crystals as starting material. Polyethylene single crystals reacted with fuming nitric acid resulting in long methylene chains with functional groups such as COOH and NO(2) at the ends (the chain length were almost same as the lamellar thickness of polyethylene single crystal). The functionalized methylene chains were allowed to react with 4-aminostyrene to give corresponding amides, i.e., methylene chains with vinyl groups at the ends (macromer). The macromers were extended by block-copolymerization with vinyl acetate, then saponified resulting in PE/PVA block co-polymer. The block copolymer was molded into sheets which were subsequently heat-treated in contact with hydrophilic or hydrophobic media. Depending on the media, the sheet surface changed at high temperature reversibly from hydrophilic to hydrophobic and vice versa. The surface property was fixed by quenching because both blocks were able to crystallize. Thus the surface of this material can be tailored for various purposes at high temperature, and then used in stable at room temperature.
en-copyright=
kn-copyright=
en-aut-name=ShimamuraKaoru
en-aut-sei=Shimamura
en-aut-mei=Kaoru
kn-aut-name=“‡‘ºŒO
kn-aut-sei=“‡‘º
kn-aut-mei=ŒO
aut-affil-num=1
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=MunesawaYuhji
kn-aut-sei=Munesawa
kn-aut-mei=Yuhji
aut-affil-num=2
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=“à“c“N–ç
kn-aut-sei=“à“c
kn-aut-mei=“N–ç
aut-affil-num=3
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Applied Chemistry
affil-num=2
en-affil=
kn-affil=Department of Applied Chemistry
affil-num=3
en-affil=
kn-affil=Department of Applied Chemistry
END
start-ver=1.4
cd-journal=joma
no-vol=35
cd-vols=
no-issue=1-2
article-no=
start-page=35
end-page=40
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2001
dt-pub=20010327
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Scanning Probe Microscopy of Poly(p-phenylene benzobisthiazole) Lamellar Crystal
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Rigid polymer, poly(p-phenylene benzobisthiazole), formed lamellar crystals where the molecular chains were oriented perpendicular to the lamellae. It was supposed that, because of wide distribution in the chain length, the lamellar surface bristled with the chain cilia among which many voids were included. Crystallographically, this region
afforded us a transitional structure from full to deficient packings of chains. The structure was analyzed using the scanning probe microscope. In the course the method for imaging one molecular chain end was developed. From the images it was concluded that an isolated long cilius did not move so violently at room temperature.
en-copyright=
kn-copyright=
en-aut-name=ShimamuraKaoru
en-aut-sei=Shimamura
en-aut-mei=Kaoru
kn-aut-name=“‡‘ºŒO
kn-aut-sei=“‡‘º
kn-aut-mei=ŒO
aut-affil-num=1
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=“à“c“N–ç
kn-aut-sei=“à“c
kn-aut-mei=“N–ç
aut-affil-num=2
ORCID=
en-aut-name=InoueTomohiro
en-aut-sei=Inoue
en-aut-mei=Tomohiro
kn-aut-name=ˆäã’q”Ž
kn-aut-sei=ˆäã
kn-aut-mei=’q”Ž
aut-affil-num=3
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Applied Chemistry
affil-num=2
en-affil=
kn-affil=Department of Applied Chemistry
affil-num=3
en-affil=
kn-affil=Department of Applied Chemistry
END
start-ver=1.4
cd-journal=joma
no-vol=31
cd-vols=
no-issue=1
article-no=
start-page=5
end-page=10
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=19961227
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Solution-Grown Crystals of Poly(p-phenylene benzobisthiazole)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Rigid polymer poly(p-phenylene benzobisthiazole) was crystallized from dilute solution. Electron microscopy showed that upon quenching, flat fibrils with several nm thick were produced. Subsequent heat treatment in solvent changed the fibril into "shish-kebab". On the other hand, by isothermal crystallization, an aggregate of parallel rod-like crystals was obtained. The molecular chains were accommodated normal to the rod. Based on the observation of crystal morphology, the isothermal crystallization mechanism was proposed. Because of regidity of polymer chains and wide distribution of the molecular length, the chain ends were inevitably included within the crystals resulting in crystal defects such as axial shift, lattice curvature and edge dislocation which were directly observed by lattice imaging.
en-copyright=
kn-copyright=
en-aut-name=ShimamuraKaoru
en-aut-sei=Shimamura
en-aut-mei=Kaoru
kn-aut-name=“‡‘ºŒO
kn-aut-sei=“‡‘º
kn-aut-mei=ŒO
aut-affil-num=1
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=ZhangChunxiao
kn-aut-sei=Zhang
kn-aut-mei=Chunxiao
aut-affil-num=2
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=“à“c“N–ç
kn-aut-sei=“à“c
kn-aut-mei=“N–ç
aut-affil-num=3
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Applied Chemistry
affil-num=2
en-affil=
kn-affil=Department of Applied Chemistry
affil-num=3
en-affil=
kn-affil=Department of Applied Chemistry
END
start-ver=1.4
cd-journal=joma
no-vol=31
cd-vols=
no-issue=1
article-no=
start-page=11
end-page=17
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=19961227
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Synthesis and Characterization of Poly[(benzo[1,2-d:5,4-d']bisthiazole-2,6-diyl)-1,4-phenylene]
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Poly[(benzo[1,2-d:5,4-d']bissthiazole-2,6-diyl)-1,4-phenylene](cis-PBZT)with a relatively high molecular weight was prepared by a new synthesis route. Properties of the synthesized polymer, such as thermostability, liquid crystallinity etc. were investigated and compared with those of trans-PBZT. cis-PBZT was crystallized from dilute solution and the electron microscopy showed that the precipitate was a rod-like crystal similar to that of trans form. In spite of rigid nature of the back bone, cis-PBZT showed poor crystallinity.
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=ZhangChunxiao
kn-aut-sei=Zhang
kn-aut-mei=Chunxiao
aut-affil-num=1
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=TanigawaSatoshi
kn-aut-sei=Tanigawa
kn-aut-mei=Satoshi
aut-affil-num=2
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=“à“c“N–ç
kn-aut-sei=“à“c
kn-aut-mei=“N–ç
aut-affil-num=3
ORCID=
en-aut-name=ShimamuraKaoru
en-aut-sei=Shimamura
en-aut-mei=Kaoru
kn-aut-name=“‡‘ºŒO
kn-aut-sei=“‡‘º
kn-aut-mei=ŒO
aut-affil-num=4
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Applied Chemistry
affil-num=2
en-affil=
kn-affil=Department of Applied Chemistry
affil-num=3
en-affil=
kn-affil=Department of Applied Chemistry
affil-num=4
en-affil=
kn-affil=Department of Applied Chemistry
END