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ID 61291
Author
Tumewu, Stephany Angelia Graduate School of Environmental and Life Science, Okayama University
Ogawa, Yujiro Faculty of Agriculture, Okayama University
Okamoto, Takumi Faculty of Agriculture, Okayama University
Sugihara, Yuka Faculty of Agriculture, Okayama University
Yamada, Hajime Faculty of Agriculture, Okayama University
Taguchi, Fumiko Graduate School of Environmental and Life Science, Okayama University
Matsui, Hidenori Graduate School of Environmental and Life Science, Okayama University
Yamamoto, Mikihiro Graduate School of Environmental and Life Science, Okayama University Kaken ID publons researchmap
Noutoshi, Yoshiteru Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Toyoda, Kazuhiro Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Ichinose, Yuki Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Abstract
Pseudomonas syringae pv. tabaci 6605 (Pta6605) is a causal agent of wildfire disease in host tobacco plants and is highly motile. Pta6605 has multiple clusters of chemotaxis genes including cheA, a gene encoding a histidine kinase, cheY, a gene encoding a response regulator, mcp, a gene for a methyl-accepting chemotaxis protein, as well as flagellar and pili biogenesis genes. However, only two major chemotaxis gene clusters, cluster I and cluster II, possess cheA and cheY. Deletion mutants of cheA or cheY were constructed to evaluate their possible role in Pta6605 chemotaxis and virulence. Motility tests and a chemotaxis assay to known attractant demonstrated that cheA2 and cheY2 mutants were unable to swarm and to perform chemotaxis, whereas cheA1 and cheY1 mutants retained chemotaxis ability almost equal to that of the wild-type (WT) strain. Although WT and cheY1 mutants of Pta6605 caused severe disease symptoms on host tobacco leaves, the cheA2 and cheY2 mutants did not, and symptom development with cheA1 depended on the inoculation method. These results indicate that chemotaxis genes located in cluster II are required for optimal chemotaxis and host plant infection by Pta6605 and that cluster I may partially contribute to these phenotypes.
Keywords
Bacterial virulence
cheA
Chemotaxis
cheY
Flagellar motility
Pseudomonas
Note
This is a post-peer-review, pre-copyedit version of an article published in Molecular Genetics and Genomics. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00438-020-01745-y.
This fulltext is available in Jan. 2022.
Published Date
2021-01-02
Publication Title
Molecular Genetics and Genomics
Volume
volume296
Publisher
Springer
Start Page
299
End Page
312
ISSN
1617-4615
NCID
AA11547886
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
File Version
author
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1007/s00438-020-01745-y
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
助成番号
26660035
19H02956