ID | 34114 |
FullText URL | |
Author |
Sasabe, Michiko
Naito, Kana
Suenaga, Hiroko
Ikeda, Takako
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Abstract | The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 ( NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow ( BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein ( GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells. |
Keywords | defense response
elicitin
lectin
receptor-like kinase
tobacco BY-2
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Note | Published with permission from the copyright holder.
This is published in DNA Sequence, 2007 Vol.18 Issue.2 pp.152-159 Publisher URL: http://dx.doi.org/10.1080/10425170601060905 Direct access to Thomson Web of Science record (C)2008 by Informa plc. |
Published Date | 2007-01-01
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Publication Title |
DNA Sequence
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Volume | volume18
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Issue | issue2
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Start Page | 152
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End Page | 159
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Content Type |
Journal Article
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language |
English
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Refereed |
True
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DOI | |
Web of Science KeyUT | |
Submission Path | microbiology_and_immunology/11
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