ID | 57955 |
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Author |
Saigusa, Daisuke
Tohoku Medical Megabank Organization, Tohoku University
Hyodo, Yuki
Department of Child Neurology, Okayama University Hospital
Umeda, Keiko
Tohoku Medical Megabank Organization, Tohoku University
Saijo, Reina
Tohoku Medical Megabank Organization, Tohoku University
Koshiba, Seizo
Tohoku Medical Megabank Organization, Tohoku University
Kobayashi, Katsuhiro
Department of Child Neurology, Okayama University Hospital
Kaken ID
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Abstract | To characterize metabolic profiles within the central nervous system in epilepsy, we performed gas chromatography-tandem mass spectrometry (GC-MS/MS)-based metabolome analysis of the cerebrospinal fluid (CSF) in pediatric patients with and without epilepsy. The CSF samples obtained from 64 patients were analyzed by GC-MS/MS. Multivariate analyses were performed for two age groups, 0-5 years of age and 6-17 years of age, to elucidate the effects of epilepsy and antiepileptic drugs on the metabolites. In patients aged 0-5 years (22 patients with epilepsy, 13 without epilepsy), epilepsy patients had reduced 2-ketoglutaric acid and elevated pyridoxamine and tyrosine. In patients aged 6-17 years (12 with epilepsy, 17 without epilepsy), epilepsy patients had reduced 1,5-anhydroglucitol. Valproic acid was associated with elevated 2-aminobutyric acid, 2-ketoisocaproic acid, 4-hydroxyproline, acetylglycine, methionine, N-acetylserine, and serine. Reduced energy metabolism and alteration of vitamin B6 metabolism may play a role in epilepsy in young children. The roles of 1,5-anhydroglucitol in epilepsy in older children and in levetiracetam and zonisamide treatment remain to be explained. Valproic acid influenced the levels of amino acids and related metabolites involved in the metabolism of serine, methionine, and leucine.
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Keywords | antiepileptic drugs
gas chromatography-tandem mass spectrometry
metabolome analysis
metabolomics
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Amo Type | Original Article
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Publication Title |
Acta Medica Okayama
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Published Date | 2020-02
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Volume | volume74
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Issue | issue1
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Publisher | Okayama University Medical School
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Start Page | 65
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End Page | 72
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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Copyright Holders | CopyrightⒸ 2020 by Okayama University Medical School
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File Version | publisher
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Refereed |
True
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