Blood cells of schizophrenics differ in many points from those of normal subjects. First of all the shape of them is flat and thin. This tendency is more marked in old group than in new group; the volume is small; flat corpuscles are more numerous in them in the normal; and the blood resistance against diluted saline solution is stronger than that of the normal. It has long since been known that the rate of corpuscle sedimentation is being accelerated in schizophrenics. A simple physical cause that blood corpuscles are flat and numerous can explain this phenomenon. It is said that there is an antisphering substance among the factors controlling the thickness and roundness of blood corpuscles. Yet it has not been determined whether this substance on the surface of the blood of schizophrenics is large or small. Blood corpuscles are said to lose their peculiar disc-shape and to be completely destroyed at the pH of 9.2 when the antisphering substance is removed from the surface of blood corpuscles. The lower the pH is the better is the absorption of this substance on the surface of blood corpuscles; and it seems that the more this substance attaches itself to blood corpuscles the greater is the degree of flatness and in this connection it is interesting to note that the pH of schizophrenic blood is low in low in general. On the other hand, however, sphericity is increased at the time when the acidity of blood is increased due to a sudden movement of acidic substances immediately after ECT. Again in the case of coma of insulin treatment, blood tends to be alkaline and even then an increase in the sphericity of corpuscles is indicated. Consequently it seems tnat the roundness of blood corpuscles is not solely dependent upon antisphering substance and pH.
en-copyright= kn-copyright= en-aut-name=KosakaMutsutoshi en-aut-sei=Kosaka en-aut-mei=Mutsutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=4 article-no= start-page=227 end-page=252 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195609 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on bile pigments IV. Direct bilirubin in canine bile en-subtitle= kn-subtitle= en-abstract= kn-abstract=Two forms of the direct bilirubin separated from the dried canine cholecystic bile were subjected to paper chromatography and emission or infra-red spectroscopy, and the following results were obtained: 1. The two forms of the direct bilirubin contain plenty of bile acid or its salt, and benzidine- and ninhydrine-positive substances together with various inorganic elements were also detected. 2. The ester-form bilirubin had carboxyl radical by infrared spectroscopy. But it will not be easily concluded that an existence of carboxyl radical will owe to free carboxyl radical of the dibasic acid bilirubin by the reason why an existence of plenty of bile acid in the sample may inhibit the characteristic absorption of ester. 3. It may be suggested that the two forms of the direct bilirubin combine with bile acid or its salt, and that the affinity between them is stronger in the salt-form bilirubin. 4. It seems probable that properties of the salt-form and ester-form bilirubins are not influenced by an existence of bile acid or its salt, and further by acornbination with it.
en-copyright= kn-copyright= en-aut-name=SakamotoTakeshi en-aut-sei=Sakamoto en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=4 article-no= start-page=253 end-page=260 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195609 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on bile pigments V. A method to isolate indirect bilirubin en-subtitle= kn-subtitle= en-abstract= kn-abstract=For the purpose of obtaining the dibasic acid indirect bilirubin in a pure state from the dried canine cholecystic bile, an optimal developing solvent was selected by paper partition chromatography as a preliminary experiment, and it was isolated on cellulose column as an applied experiment. 1. The dibasic acid indirect bilirubin was separable at the starting point in a pure state by paper chromatography under development with the top layer of a n-butanol, acetic acid, water mixture (4:1:5). 2. The dibasic acid indirect bilirubin formed a fixed band at the upper starting place on cellulose column under development with the top layer of a n-butanol, acetic acid, water mixture (4:1:5), and no other substance could be detected there. 3. The dibasic acid indirect bilirubin existing in the fixed band could be eluted out into chloroform with a 1% acetic acid solution. An orange yellow powder was obtained from the eluate by evaporating the solvent in vacuo. 4. Thus separated orange yellow powder agreed well with the crystalline bilirubin in the solubility into organic or inorganic solvents and in the spectrochemical characteristics as well as in the chemical properties.
en-copyright= kn-copyright= en-aut-name=SakamotoTakeshi en-aut-sei=Sakamoto en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=4 article-no= start-page=175 end-page=213 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195609 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Pathology of "landry-guillain-barré syndrome" in children, prevailing in the regions surrounding the Inland Sea of Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=Histopathological investigations were carried out on five fatal cases of a type of polyneuritis of unknown etiology diagnosed as Landry-Guillain-Barre syndrome, which endemically occurred in children in the regions surrounding the Inland Sea of Japan. The most characteristic pathologic feature in the nervous system was pronounced patchy degenerative changes with slight or moderate degree of inflammatory cell response of focal type in the peripheral and cranial nerves, predominantly in the nerve fibers of the spinal and cranial roots. In the spinal cord, medulla, pons, and in some portions of the cerebrum and cerebellum, engorgement of the small blood vessels as well as edema and the less predominant scattered degenerative changes of ganglioncells and nerve fibers with extremely slight degree of glial response and sparse perivascular cell collections were encountered. The cerebrospinal meninges displayed edema and congestion of the pial blood vessels with focal collections of a small number of lymphocytes and/or monocytes. No advanced involvement of the anterior horn of the spinal cord in a strict sense of anterior poliomyelitis was, however, recognized. These changes may lead the histopathologic diagnosis of the present disease to infectious encephalomyelo-polyradiculoneuritis or a type of infectious polyneuritis. The main histopathologic changes in the visceral organs were a moderate degree of engorgement of the small blood vessels, degeneration of parencymatous organs such as the liver and kidney, hyperplasia or follicular atrophy of the lymphatic tissues, interalveolar pneumonia, focal myositis, and slight degree of round cell infiltrations in the interstitial tissues of the other viscera, such as the liver, heart, and gastrointestinal canal. Based upon the observations on the histopathological changes as well as clinical manifestations, discussions were made on the pathogenesis and etiologic factor of the present endemic disease with critique on the literatures.
en-copyright= kn-copyright= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkagiSeiji en-aut-sei=Akagi en-aut-mei=Seiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=2 article-no= start-page=62 end-page=69 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195604 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=On the Inoculation of the HST (Hamazaki) Virus Isolated From Yoshida Tumor on Emb-ryonated Eggs en-subtitle= kn-subtitle= en-abstract= kn-abstract=HST virus which was isolated in 1950 from the roshida ascites tumor by Hamazaki and his associates is a pantropic virus which creates a unique inflammatory granulation in mice. When virus of an acute infections disease was inoculated on embryonated eggs, not only the egg membrane but also the chick embryo were infected more or less, and when the number of virus increased the chick embryo died, terminating the development of the egg. However, the tumor inducing virus which represents the Rous virus does not cause heavy disturbances in the embryo and it is well known that chick hatched from this egg can long maintain health unless it is subjected to a provocative factor. HST virus is no exception to this example and though it is inoculated on an embryonated egg it does not cause any serious disturbance on the embryo. The tissue changes of the chorio allantois infected by the "Virus were the focal proliferation and necrosis of ectodermal epithelium, the proliferation of the mesenchymal cells of the mesodermal layer adjacent to these foci, accompaning infiltration of lymphoid cells and leukocytes with edema, especially eosinophilic leukocytes. By these tissue changes a terrace-shaped thickening of the membrane was the result. In the viscera of the chick embryo a special change in the liver was seen, i. e., along the edge of the liver greyish white nodules submacroscopic to miliary in size appeared. The principal pathologic change of the foci is the coagulation necrosis of the liver parenchyma and only a slight infiltration about the periphery of the foci was observed. Moreover, proliferation of mesenchymal cells occurred next to the walls of the large blood vessels of the liver (principally, the portal veins) and with the added infiltration of a small number of lymphoid cells and leukocytes sharply defined nodular foci were formed. Though this was a rare instnace, similar pathologic changes were seen also in the walls of the blood vessels of the cerebrum stem of the embryo and along the periphery local gliosis was observed.
en-copyright= kn-copyright= en-aut-name=HamazakiYukio en-aut-sei=Hamazaki en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshiiShusaku en-aut-sei=Ishii en-aut-mei=Shusaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KajiyamaYutaka en-aut-sei=Kajiyama en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=2 article-no= start-page=82 end-page=88 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195604 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The Fate in Guinea-Pigs of Intraven-ously Injected I131-γ-Globulin en-subtitle= kn-subtitle= en-abstract= kn-abstract=1) The fate and rate of degradation of I131 labelled rabbit γ-globulin, which retained its native antigenicity and antibody specificity was studied in the guinea-pigs. 2) Blood elimination rate of heterologous γ-globulin is higher than that of homologous γ-globulin. 3) Denatured and digested γ-globulin departs from the blood more rapidly than the native one, and urinary excretion rates of denaturated and digested γ-globulin are higher than that of the native one. It is inferred, therefore, that the denatured and digested γ-globnlin is more liable to be resolved and decomposed in the reticulo-endothelial organs than the native one. And the value obtained from the urinary excretion reflects the rate of protein break down in some cellular compartments. 4) Following the plasmaphresis the increase in antigen elimination was lessened and delayed as compared with control animals. 5) The organ distribution of heterologous I131-γ-globulin is to the lymphnode> the spleen> the liver> the lung> the kidney> the intestine in descending order. Heterologous I 131 -γ- globulin is deposited in greater quantity in the reticulo-enclotherial organ than other single organ. 6) Following the intravenous injection of I131 labelled antigens, the ratio of the specific activity of mitochondria and microsom to that of whole liver homogenate was determined over a period from 15 minutes to 3 hours in guinea-pigs, and following results were obtained. a) Organ and intracellular distribution of Il3l labelled homologous γ-globulin shows no great difference compared to that of heterologous one. b) The intracellular distribution of heterologous γ-globulin is in mitochondrial> microsomal> nuclear fraction in descending order. c) The heterologous γ-globulin quantity of mitochondrial fraction or microsomal fraction in the spleen is higher than that of the liver. 7) The antibody distribution of intracellular glanules measured in terms of radioactivity with a Geiger-Muller counter, after the reaction of I131 labelled antigen. The quantity of distribution of intracellular glanules decreases in mitochondrial fraction> microsomal fraction> nuclear fraction in descending order.
en-copyright= kn-copyright= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MochizukiYoshio en-aut-sei=Mochizuki en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=2 article-no= start-page=82 end-page=88 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195604 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on the Inhibitory Action of Sugar and Polyatomic Alcohol for the Heat-inactivation of Complement en-subtitle= kn-subtitle= en-abstract= kn-abstract=1) When guinea pig serum was warmed at about 65°C, part of its protein became denatured resulting in the formation of a colloidal component C, which has approximately the same electrophoretic mobility as that of α-globulin. 2) The electrophoretic examination shows that the serum heated at 65°C after adding the glucose is devoid of colloidal component C. 3) The inhibitory action of sugar and polyatomic alcohol on the heat inactivation of complement is galactose> saccharose > glucose> sorbit > mannit > glycocoll> glycerin in descending order. The inhibitory action of sugar and poly atomic alcohol on molecular aggregation by heating is of the same order as complement activity.
en-copyright= kn-copyright= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MochizukiYoshio en-aut-sei=Mochizuki en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=2 article-no= start-page=57 end-page=61 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195604 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=On the function of the Megakaryocyte (Motility, Separation of the Platelet and Phagocytosis), Observations Both in Idiopathic Thrombo-cytopenic Purpura and in Normal Adult en-subtitle= kn-subtitle= en-abstract= kn-abstract=The idiopathic thrombocytopenic purpura was described by Werlhof as an independent disease first in 1738. Kaznelson reported the excellent effect of splenectomy for its chronic type in 1915. For the genesis of its thrombocytopenia, there have been many theories to be concluded into the followings, 1) the development of an auto-immune mechanism resulting in platelet destruction, 2) increased platelet destruction in the spleen, 3) the inhibition of platelet production from the marrow megakaryocytes by a humoral factor produced in the spleen, 4) both increased destruction and decreased production of the platelet. Among the above four theories, the third one is the most popular in the chronic type.
en-copyright= kn-copyright= en-aut-name=HirakiK. en-aut-sei=Hiraki en-aut-mei=K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OfujiT. en-aut-sei=Ofuji en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KobayashiT. en-aut-sei=Kobayashi en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SunamiH. en-aut-sei=Sunami en-aut-mei=H. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=AwaiK. en-aut-sei=Awai en-aut-mei=K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=2 article-no= start-page=89 end-page=98 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195604 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A Simplified Hot-Plate Apparatus for Evaluation of Analgesic Effect en-subtitle= kn-subtitle= en-abstract= kn-abstract=1. A. simple hot-plate apparatus, with comparatively good accuracy in the control of temperature, chiefly composed of a circular lead plate embedded with nicrome-wire heater was devised in order to improve the complications and inconveniences of the hot-plate apparatus used to date in algesimetric determination with mice. 2. The reaction times measured with this hot-plate maintained at 55°C with 500 normal mice showed an average of 9.96 ± 1.58 seconds and about 90% of the total mice exhibited reaction time of 7-13 seconds. With animals showing reaction time within this range, the daily mean reaction time did not vary with measurement once a day for consecutive days and the effect of drugs with comparatively weak analgesic effect was well reproduced with small number examples, such as 12 mice to a group.
en-copyright= kn-copyright= en-aut-name=SanukiKazumasa en-aut-sei=Sanuki en-aut-mei=Kazumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhnoHiroyuki en-aut-sei=Ohno en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=3 article-no= start-page=110 end-page=129 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195607 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Observations of various living blood cells by tissue culture of the bone marrow en-subtitle= kn-subtitle= en-abstract= kn-abstract=(1) The movement of the blood cells in the bone marrow was classified into 9 types. (2) The characteristics of moving types are so distinct according to the kinds of blood cells, that the differential diagnosis of the cells by moving types is easily and certainly made. In this way, (by the kind of blood cells), we have classified leukemia, as is described in our other articles. (3) The phagocytosis and vital staining of the blood cells in the bone marrow is different in degree and mode, according to the kind of blood cells, and thus becomes valuable ground for the differential diagnosis of the cells.
en-copyright= kn-copyright= en-aut-name=HirakiKiyoshi en-aut-sei=Hiraki en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OfujiTadashi en-aut-sei=Ofuji en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatariZenji en-aut-sei=Watari en-aut-mei=Zenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=3 article-no= start-page=130 end-page=150 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195607 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Diagnosis of leukemia and aplastic anemia by the bone marrow culture en-subtitle= kn-subtitle= en-abstract= kn-abstract=1) As the boundary of the outgrowth zone of the bone marrow culture is sharply defined from the surrounding area and the cellular density becomes very high in leukemia, diagnosis can be made with ease and certainty. 2) The differential diagnosis between aleukemic leukemia and aplastic anemia becomes easy by application of the tissue cuIture method. 3) The vital observation of blood cells were used in classification of leukemia by cellular systems, and it was discovered that there were a lot of patients with monocytic leukemia and relatively many with acute lymphatic leukemia. 4) Because the cellular growth in the bone marrow culture of aplastic anemia declines extremely and abundant fat cells are observed, the diagnosis of this disease is easily made by employment of this method.
en-copyright= kn-copyright= en-aut-name=HirakiKiyoshi en-aut-sei=Hiraki en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OfujiTadashi en-aut-sei=Ofuji en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HattoriYoshiyuki en-aut-sei=Hattori en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=3 article-no= start-page=151 end-page=163 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195607 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=On the excitation and inhibition of the inspiratory neuron in the respiratory centers en-subtitle= kn-subtitle= en-abstract= kn-abstract=In decerebrated cats the impulse discharges were detected by means of an unipolar microelectrode from a single inspiratory neuron in the respiratory centers, and the change in discharge produced by administration of stimulating, as well as depressant agents, was studied. The results were summarized as follows. 1) Inhalation of the air containing all. excess of carbon dioxide, as well as the air deficient of oxygen and the intravenous administration of a small dose of caffeine, aminocordine and lobeline produced a) a remarkable increase of frequency of impulses in the stage of constant frequency of the volley, b) a rapid increase of frequency of impulses in the stage of crescent frequency of the volley and c) a shortening of the duration of the volleys, as well as of silent periods. 2) In narcosis by a moderate dose of morphine, as well as in the recovery stage from apnea produced by over-ventilation, there were observed the phenomena which were exactly opposite to those described in 1). 3) It was concluded that a most essential sign by which one can discern whether the activity of the respiratory Genters is raised or depressed, is the changes of the frequency of impulses produced from an inspiratory neuron. The expense of this research was defrayed from the grant in aid of the Ministry of Education.
en-copyright= kn-copyright= en-aut-name=HukuharaTakesi en-aut-sei=Hukuhara en-aut-mei=Takesi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkadaHiromasa en-aut-sei=Okada en-aut-mei=Hiromasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=3 article-no= start-page=99 end-page=109 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195607 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The method of tissue culture (mainly of the bone marrow) and a simple method of observing living tissue en-subtitle= kn-subtitle= en-abstract= kn-abstract=Our methods of tissue culture of the bone marrow, lymphnodes, and peripheral blood were described. Furthermore, for the purpose of promoting wide clinical application of bone marrow tissue culture, our simple vital inspection method was also stated.
en-copyright= kn-copyright= en-aut-name=HirakiKiyoshi en-aut-sei=Hiraki en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OfujiTadashi en-aut-sei=Ofuji en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SunamiHiroshi en-aut-sei=Sunami en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=3 article-no= start-page=164 end-page=172 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195607 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The effect of an antihistamine agent on the gastric secretion induced by sinomenine and irgapyrin en-subtitle= kn-subtitle= en-abstract= kn-abstract=1. Sinomenine and Irgapyrin, the two antirheumatics known to be capable of releasing histamine, caused a marked gastric secretion in the unanesthetized dog. 2. The facial edema and itching associated with histamine release by sinomenine was almost completely eliminated by NeoAntergan, but the gastric secretion was not suppressed, or rather increased - an observation also reported by Paton and Schachter with Compound 48/80. This indicates that the histamine release cannot be markedly prevented by antihistamine agents in this animal. 3. The gastric secretion induced by Irgapyrin was not suppressed by Neo-Antergan but Irgapyrin originally never caused other symptoms associated with histamine release. This is probably due to the antihistamine action inherent in this compound itself. 4. No such histamine-releasing activity, as determined by gastric secretion, could be observed in aminopyrine or butazolidine sodium, the components of Irgapyrin. 5. Sinomenine, differing from Irgapyrin and Compound 48/80, was ineffective by intramuscular injection.
en-copyright= kn-copyright= en-aut-name=NishiyamaRyosaku en-aut-sei=Nishiyama en-aut-mei=Ryosaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=1 article-no= start-page=6 end-page=10 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195601 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of Radioactive P32 Upon Japanese B Encephalitis in Mice en-subtitle= kn-subtitle= en-abstract= kn-abstract=<P>The effect of P³² in the experimental Japanese B Encephalitis infection was investigated. A remarkable delay of infection was observed, when a therapeutic dose of P³² was administered intraperitoneally to the mouse which had been inoculated intracerebrally with the virus suspension at the concentration of about LD50. Almost equal results were obtained by intraperitoneal administrations to the mice when they had deen inoculated intravenously or intraperitoneally.
en-copyright= kn-copyright= en-aut-name=OsadaTakahisa en-aut-sei=Osada en-aut-mei=Takahisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=1 article-no= start-page=11 end-page=29 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195601 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on Bile Pigments I. Separation of Natural Indirect Bilirubins en-subtitle= kn-subtitle= en-abstract= kn-abstract=Descriptions are carried on the method how to separate the indirect bilirubin from the chloroform extracts of the dried dog's gallbeadder bile by adsorption chromatography. 1. The optimal concentrations of the bilirubin content were 2 to 4 mg/100 ml when 1 ml of the sample was adsorbed on the Tswett tube of about 10 mm diameter. 2. Though several zones of the indirect bilirubin were separated on the column of silica gel when developed with various solvents, these zones were proved to be mingled with some oxidized or other intermediate products and the separation like this was thought to owe to the activity of the adsorbents. 3. The chromatogram of the crystalline bilirubin resembled to the one formed by the indirect bilirubin in the chloroform extracts. 4. The chromatogram of the chromatographically separated indirect bilirubin was similar to the former. 5. The absorption maxima of a chloroform solution of the natural indirect bilirubin existed at 450 mμ in the visible range, and it was the same as the maxima of the crystalline bilirubins.
en-copyright= kn-copyright= en-aut-name=SakamotoTakeshi en-aut-sei=Sakamoto en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=1 article-no= start-page=30 end-page=46 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195601 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on Bile Pigments II. Seperation of Natural Direct Bilirubins en-subtitle= kn-subtitle= en-abstract= kn-abstract=Separation of both forms of the direct bilirubin were carried out from the dog's gallbladder bile, and further isolations of them were also done. 1. The natural salt-form bilirubin was isolated after separation on the column of aluminium oxide with a n-propanolic aqueous solution. 2. The natural salt-form bilirubin was obtained in amorphous yellow powders which were strongly hygroscopic and easily soluble in water and methanol but not in chloroform or carbon tetrachloride. An aqueous solution of these powders showed both the direct diazo and Gmelin reaction, but neither Ehrlich's aldehyde nor Schlesinger reaction. The salt-form bilirubin was transferred into chloroform only when some quantities of hydrochloric acid were added to a mixture of chloroform and an aqueous solution of it. 3. The absorption maxima of the natural salt-form bilirubin existed at 420 to 430 mμ in a methanolic solution and at 425 or 435mμ in 50% or 10% n-propanol. 4. The natural ester-form bilirubin was isolated after separating on the column of silica gel with a chloroformethanolic mixture. 5. The natural ester-form bilirubin was obtained in amorphous greenish yellow powders. It was further hygroscopic and easily soluble in water and methanol but not in chloroform or carbon tetrachloride. An aqueous solution of it showed the direct diazo and Gmelin reaction, but neither Ehrlich's aldehyde nor Schlesinger's reaction. No pigment was transferred into chloroform even if some quantities of hydrochloric acid were added to a mixture of chloroform and an aqueous solution of it, but did by saponification with 5% methanolic potash. 6. The absorption maxima of the natural ester-form bilirubin existed at 415 mμ in both methanolic and aqueous solutions.
en-copyright= kn-copyright= en-aut-name=SakamotoTakeshi en-aut-sei=Sakamoto en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=1 article-no= start-page=1 end-page=5 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195601 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Diacetyl as Acetyl Donor en-subtitle= kn-subtitle= en-abstract= kn-abstract=Diacetyl can serve as an acetyl donor for the forlnation of citrate and the acetylation of sulfanilamide in the dog heart homogenate. Diphosphothiamine and coenzyme A are essential for these reactions.
en-copyright= kn-copyright= en-aut-name=ShinagawaToshiro en-aut-sei=Shinagawa en-aut-mei=Toshiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujiiTakao en-aut-sei=Fujii en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OoishiTetsuya en-aut-sei=Ooishi en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=1 article-no= start-page=47 end-page=55 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195601 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on Bile Pigments III. Biliverdins Derived From Natural Bilirubins en-subtitle= kn-subtitle= en-abstract= kn-abstract=1. Absorption maxima of hydrochloric biliverdins derived from the natural indirect bilirubin existed at 680 mμ and 375 mμ, but the maxima of biliverdins purified on the column of silica gel existed at 640 mμ and 390 mμ. 2. The natural salt-form bilirubin was oxidized by hydrochloric acid to biliverdin, of which absorption maxima existed at 685 mμ and 370 mμ in a methanolic solution as well as in 5% hydrochloric methanol, but the purified biliverdin in chloroform solution showed the maxima at 640 mμ and 390 mμ. 3. The natural ester-form bilirubin could be transformed into biliverdin by oxidation of its alcoholic solution in the presence of hydrochloric acid. The crude biliverdin had absorption maxima at 645 to 655 mμ, 600 mμ and 320 mμ, and the crude hydrochloric biliverdin had the maxima at 665 to 675 mμ, 620 mμ and in the near ultra-violet range, while the purified biliverdin in chloroform solution had the maxima at 640 mμ and 380 mμ. 4. The biliverdins derived from the indirect, salt-form and ester-form bilirubin had quite similar absorption maxima after purifications by adsorption chromatography.
en-copyright= kn-copyright= en-aut-name=SakamotoTakeshi en-aut-sei=Sakamoto en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=367 end-page=374 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The inhibition of substance P-induced histamine release from mast cells by 6, 7-dihydro-6, 8, 8, 10-tetramethyl-8H-pyrano-[3, 2-g] chromone-2-carboxylic acid (EAA). en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the presence of extracellular Ca2+, 6,7-dihydro-6,8,8, 10-tetramethyl-8H-pyrano [3, 2-g] chromone-2-carboxylic acid (EAA) had an inhibitory effect on the substance P-induced histamine release from rat peritoneal mast cells. Not only Ca2+ but also Mg2+, Sr2+ and Ba2+ were effective in enhancing the activity of EAA. Marked tachyphylaxis to EAA developed irrespective of the presence or absence of extracellular Ca2+. Cross-tachyphylaxis was observed between EAA and disodium cromoglycate (DSCG). These results indicate that the mode of action of EAA is similar, but not identical, with that of DSCG.
en-copyright= kn-copyright= en-aut-name=TsutsumiKoji en-aut-sei=Tsutsumi en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishiboriMasahiro en-aut-sei=Nishibori en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SaekiKiyomi en-aut-sei=Saeki en-aut-mei=Kiyomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univresity en-keyword=6 kn-keyword=6 en-keyword=7-dihydro-6 kn-keyword=7-dihydro-6 en-keyword=8 kn-keyword=8 en-keyword=8 kn-keyword=8 en-keyword=10-tetramethyl-8H-pyrano(3 kn-keyword=10-tetramethyl-8H-pyrano(3 en-keyword= 2-g) chromone-2-carboxylic acid (EAA) kn-keyword= 2-g) chromone-2-carboxylic acid (EAA) en-keyword=disodium cromoglycate kn-keyword=disodium cromoglycate en-keyword=histamine release kn-keyword=histamine release en-keyword=alkaline-earth metal ions kn-keyword=alkaline-earth metal ions en-keyword=substance P kn-keyword=substance P END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=375 end-page=380 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Transaminative metabolism of L-cysteine in guinea pig liver and kidney. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Transaminative metabolism of L-cysteine was investigated using homogenates of guinea pig liver and kidney. L-Cysteine was transaminated in the presence of 2-oxoglutarate and the homogenate of either liver or kidney. S-(2-Hydroxy-2-carboxyethylthio)cysteine (HCETC) (3-mercaptolactate-cysteine disulfide) was formed by liver homogenate, but the amount was very small. On the other hand, a relatively large amount of HCETC was formed in the presence of kidney homogenate. Transamination between 3-mercaptopyruvate and certain amino acids was catalyzed actively by both liver and kidney homogenates in the presence of L-glutamate. However, more half-cysteine was formed by liver than kidney, and more HCETC was produced by kidney than liver. L-Glutamate was the most potent amino donor, and L-aspartate strongly inhibited the reaction. Results indicate that L-cysteine can be transaminated both in liver and kidney of the guinea pig, and that kidney is more active than liver. 2-Oxoglutarate is the most active 2-oxo acid for cysteine transamination. Oxaloacetate (and aspartate in the reverse reaction) is inhibitory to the reaction. These results are in agreement with the previous conclusion that cysteine aminotransferase is identical with aspartate aminotransferase.
en-copyright= kn-copyright= en-aut-name=TaniguchiMiyabi en-aut-sei=Taniguchi en-aut-mei=Miyabi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HosakiYasuhiro en-aut-sei=Hosaki en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UbukaToshihiko en-aut-sei=Ubuka en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=cysteine kn-keyword=cysteine en-keyword=transamination kn-keyword=transamination en-keyword=guinea pig kn-keyword=guinea pig en-keyword=mercaptopyruvate kn-keyword=mercaptopyruvate en-keyword=mercaptolactate-cysteine disulfide kn-keyword=mercaptolactate-cysteine disulfide END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=409 end-page=413 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Electrophoresis and electro-affinity transfer with specific antibodies to alpha-fetoprotein for detection of circulating immune complexes of alpha-fetoprotein. en-subtitle= kn-subtitle= en-abstract= kn-abstract=<p>A combination of agarose gel electrophoresis and a newly developed technique of electro-affinity transfer was applied to the detection of circulating immune complexes of human alpha-fetoprotein (AFP) and anti-AFP. After electrophoretic transfer to nitrocellulose membrane, to which affinity-purified polyclonal horse antibodies to human AFP were bound, the membranes were treated with or without rabbit immunoglobulins to human AFP, followed by overlaying with horseradish peroxidase-labeled goat anti-rabbit IgG for color development. Artificial complexes formed in vitro from human AFP and rabbit anti-AFP were clearly separated from free AFP by the agarose electrophoresis. The complexes were stained 20-40% as dark as the equivalent amount of free AFP by treatment with rabbit anti-AFP, and 10-20% as dark without the antibody treatment over a wide range of antigen-antibody ratios.</p>
en-copyright= kn-copyright= en-aut-name=TaketaKazuhisa en-aut-sei=Taketa en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IchikawaEriko en-aut-sei=Ichikawa en-aut-mei=Eriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TagaHiroko en-aut-sei=Taga en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HiraiHidematsu en-aut-sei=Hirai en-aut-mei=Hidematsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Kagawa University affil-num=2 en-affil= kn-affil=Kagawa University affil-num=3 en-affil= kn-affil=Tumour Laboratory affil-num=4 en-affil= kn-affil=Tumour Laboratory en-keyword=circulating immune complexes kn-keyword=circulating immune complexes en-keyword= ?-fetoprotein kn-keyword= ?-fetoprotein en-keyword=electrophoresis kn-keyword=electrophoresis en-keyword=electroaffinity transfer kn-keyword=electroaffinity transfer en-keyword=enzyme immuno-detection kn-keyword=enzyme immuno-detection END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=325 end-page=340 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Brain catecholamines in spontaneously hypertensive and DOCA-salt hypertensive rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The concentrations and alpha-methyl-p-tyrosine (alpha-MPT) induced disappearance of catecholamines, adrenaline, noradrenaline and dopamine, were measured in selected areas of the brainstem and hypothalamus of spontaneously hypertensive rats (SHR) and deoxycorticosterone acetate (DOCA)-salt hypertensive rats. The catecholamine levels were measured by a sensitive radioenzymatic assay method combined with microdissection of the rat brain. The adrenaline concentration was higher in the area A1 of young SHR, but not in adult SHR, than in age-matched control rats. Noradrenaline concentrations and the alpha-MPT induced noradrenaline disappearance were less in the rostral part of the nucleus tractus solitarii (NTS) and the nucleus hypothalamic anterior of young SHR, and in the rostral part of the NTS of adult SHR. On the other hand in DOCA-salt hypertensive rats, the concentrations of adrenaline and noradrenaline were the same as in control rats in the examined areas. The alpha-MPT induced noradrenaline disappearance was less in the rostral part of the NTS of DOCA-salt hypertensive rats. Dopamine concentrations and the alpha-MPT induced dopamine disappearance were the same in the examined areas of SHR and DOCA-salt hypertensive rats. The results suggest that SHR have a change in adrenergic neural activity in the brainstem and a decrease in noradrenergic neural activity in the brainstem and hypothalamus while DOCA-salt hypertensive rats have a decrease in noradrenergic neural activity in the brainstem. Such changes in brain catecholaminergic neurons may have played an important role in the development of hypertension in these rats.
en-copyright= kn-copyright= en-aut-name=FujinoKazuyuki en-aut-sei=Fujino en-aut-mei=Kazuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=catecholamines kn-keyword=catecholamines en-keyword=brainstem kn-keyword=brainstem en-keyword=hypothalamus kn-keyword=hypothalamus en-keyword= spontaneously hypertensive rats(SHR) kn-keyword= spontaneously hypertensive rats(SHR) en-keyword=DOCA-salt hypertensive rats kn-keyword=DOCA-salt hypertensive rats END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=389 end-page=401 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Clinical and histological features of sporadic non-A, non-B hepatitis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The incidence of hepatitis A (HA), hepatitis B (HB), and non-A, non-B hepatitis (NANBH) was 27%, 30% and 43% among 73 patients with sporadic hepatitis. Epidemiological data (geographical distribution, seasonal variation, age, sex, and occupation) were not distinguishing of the type of hepatitis. Neither intrafamilial infection nor previous contact with viral hepatitis patients could be demonstrated in the NANBH cases. Fever and jaundice were less frequent in NANBH than in HA. Maximum levels of SGPT, serum bilirubin, ZTT, and gamma-globulin were significantly lower in NANBH than in HA and HB. Ten of 29 NANBH patients (35%) presented abnormal SGPT activities for more than 6 months, and four (14%) more than 12 months. In the ten patients with prolonged courses, jaundice was more frequent and maximum levels of SGPT were higher than in patients with transient courses. Histopathologic findings were not markedly different from those of HA and HB. Bile duct damage, fatty deposition, and giant multi-nucleated cells were recognized in 6, 12, and 2 NANBH patients, respectively. There were no characteristic ultrastructural changes in NANBH.
en-copyright= kn-copyright= en-aut-name=HyodoIchinosuke en-aut-sei=Hyodo en-aut-mei=Ichinosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamadaGotaro en-aut-sei=Yamada en-aut-mei=Gotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishiharaTakashi en-aut-sei=Nishihara en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkushinHiroaki en-aut-sei=Okushin en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KinoyamaShingo en-aut-sei=Kinoyama en-aut-mei=Shingo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SakamotoYuji en-aut-sei=Sakamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TobeKazuo en-aut-sei=Tobe en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=acute hepatitis kn-keyword=acute hepatitis en-keyword=sporadic non-A kn-keyword=sporadic non-A en-keyword=non-B hepatitis kn-keyword=non-B hepatitis en-keyword=liver histopathology kn-keyword=liver histopathology en-keyword=liver ultrastructure kn-keyword=liver ultrastructure END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=341 end-page=347 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Partial purification of Simian virus 40 large T antigen by immunoaffinity chromatography and its detection by enzyme-linked immunosorbent assay. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Simian virus 40 (SV40) large T antigen was partially purified from small amounts of SV40-infected and SV40-transformed cells by immunoaffinity chromatography with high recovery. T antigen, in both crude and partially purified states, was detected rapidly by a sensitive and quantitative enzyme-linked immunosorbent assay (ELISA). Stability of the partially purified T antigen was found to increase by addition of 0.01% bovine serum albumin (BSA).
en-copyright= kn-copyright= en-aut-name=IkedaShogo en-aut-sei=Ikeda en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HatsushikaMasao en-aut-sei=Hatsushika en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShigeharaTsuguya en-aut-sei=Shigehara en-aut-mei=Tsuguya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WatanabeSekiko en-aut-sei=Watanabe en-aut-mei=Sekiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OmuraSachiko en-aut-sei=Omura en-aut-mei=Sachiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TsutsuiKen en-aut-sei=Tsutsui en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=SV40 T antigen kn-keyword=SV40 T antigen en-keyword=affinity chromatography kn-keyword=affinity chromatography en-keyword=ELISA kn-keyword=ELISA END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=349 end-page=355 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of angiotensin II, catecholamines and glucocorticoid on corticotropin releasing factor (CRF)-induced ACTH release in pituitary cell cultures. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of angiotensin II, catecholamines and glucocorticoid on CRF-induced ACTH release were examined using rat anterior pituitary cells in monolayer culture. Synthetic ovine CRF induced a significant ACTH release in this system. Angiotensin II produced an additive effect on CRF-induced ACTH release. The ACTH releasing activity of CRF was potentiated by epinephrine and norepinephrine. Dopamine itself at 0.03-30 ng/ml did not show any significant effect on ACTH release, but it inhibited CRF-induced ACTH release. Corticosterone at 10(-7) and 10(-6)M inhibited CRF-induced ACTH release. These results indicate that angiotensin II, catecholamines and glucocorticoid modulate ACTH release at the pituitary level.
en-copyright= kn-copyright= en-aut-name=MurakamiKazuharu en-aut-sei=Murakami en-aut-mei=Kazuharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HashimotoKozo en-aut-sei=Hashimoto en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=corticotropin releasing factor(CRF) kn-keyword=corticotropin releasing factor(CRF) en-keyword=ACTH release kn-keyword=ACTH release en-keyword= angiotensin ? kn-keyword= angiotensin ? en-keyword=catecholamines kn-keyword=catecholamines en-keyword=glucocorticoid kn-keyword=glucocorticoid END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=321 end-page=324 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Genetic polymorphism of factor B (Bf) in Okayama Prefecture, Japan. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The genetic polymorphism of factor B (Bf) was investigated in Okayama Prefecture, Japan. Cellogel immunofixation electrophoresis was employed according to Martin and Ziegler (1981) with minor modifications. In 316 non-blood related Japanese, the Bf was: Bf S, 70.6%; Bf FS, 27.8%; and Bf F, 1.6%. No rare variants were observed. The gene frequencies of Bfs and BfF were 0.845 and 0.155, respectively. The gene frequencies in Okayama Prefecture were quite similar to those in other districts of Japan. Considering the phenotype distribution in Japan, the Bf system might be a useful marker for personal identification and in disputed paternity cases.
en-copyright= kn-copyright= en-aut-name=AmenoSetsuko en-aut-sei=Ameno en-aut-mei=Setsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NanikawaRyo en-aut-sei=Nanikawa en-aut-mei=Ryo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=factor B kn-keyword=factor B en-keyword=polymorphism kn-keyword=polymorphism en-keyword=cellogel immunofixation electrophoresis kn-keyword=cellogel immunofixation electrophoresis END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=403 end-page=407 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Relationship of tyrosine concentration to catecholamine levels in rat brain. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Rats were fed a choline-free low protein diet for 12 or 26 weeks. In the 12-week group, the brain tyrosine concentration did not change. Dopamine levels were low in both the cerebral cortex and striatum. Norepinephrine level was low in the diencephalon. In the 26-week group, the tyrosine concentration was high in the brain. However, the dopamine and norepinephrine levels did not change in the cerebral cortex, striatum and hypothalamus. Furthermore, in another group of rats which were intraperitoneally injected with tyrosine, the brain tyrosine concentration was high, whereas the dopamine and norepinephrine levels in the hypothalamus were not significantly different from control levels.
en-copyright= kn-copyright= en-aut-name=KaneyukiTakao en-aut-sei=Kaneyuki en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MorimasaTadaomi en-aut-sei=Morimasa en-aut-mei=Tadaomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShohmoriToshikiyo en-aut-sei=Shohmori en-aut-mei=Toshikiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=brain tyrosine kn-keyword=brain tyrosine en-keyword=catecholamine kn-keyword=catecholamine en-keyword=malnutrition kn-keyword=malnutrition en-keyword=liver injury kn-keyword=liver injury END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=381 end-page=387 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of a serum factor on IgE-mediated histamine release from whole blood. en-subtitle= kn-subtitle= en-abstract= kn-abstract=IgE-mediated histamine release from whole blood was analyzed in 44 patients with bronchial asthma by observing maximum present release and dose-response curves of histamine release induced by anti-IgE and house dust extract. The maximum histamine release from whole blood induced by anti-IgE correlated with total serum IgE levels. There was a close correlation between allergen-induced release from whole blood and the serum levels of specific IgE antibodies. In the maximum histamine release from whole blood induced by both anti-IgE and allergen, the interaction with a serum factor was not clearly recognized. Effect of a serum factor was shown in the dose-response curves of anti-IgE-induced histamine release, but not in those of allergen-induced histamine release. The dose-response curves caused by anti-IgE showed that basophils from cases with a high serum IgE level require much more anti-IgE to produce maximum histamine release than basophils from cases with a low serum IgE level. The results showed that IgE molecules contained in the serum participate in anti-IgE-induced histamine release from whole blood.
en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakagawaSaburo en-aut-sei=Nakagawa en-aut-mei=Saburo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KitaniHikaru en-aut-sei=Kitani en-aut-mei=Hikaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=histamine release kn-keyword=histamine release en-keyword=whole blood kn-keyword=whole blood en-keyword=serum factor kn-keyword=serum factor en-keyword=allergen kn-keyword=allergen en-keyword=anti-IgE kn-keyword=anti-IgE END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=357 end-page=365 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Inhibition by drugs of passive cutaneous anaphylaxis-induced skin histamine decrease. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Passive cutaneous anaphylaxis (PCA) was produced in the rat with mouse IgE-rich antiserum. The effect of drugs on the PCA-induced skin histamine decrease and leakage of protein-bound dye was studied. Salbutamol (0.5 mg/kg i.v. or 1.0 mg/kg s.c.) and cromoglycate (10 mg/kg i.v.) significantly inhibited the skin histamine decrease. A combination of salbutamol (0.5 mg/kg i.v. or 1.0 mg/kg s.c.) and aminophylline (25 mg/kg i.v. or 75 mg/kg s.c.) had an additive or greater than additive effect on the histamine decrease. Salbutamol (1.0 mg/kg s.c.) inhibited the dye leakage markedly, and aminophylline (75 mg/kg s.c.) slightly. These results indicate that the decrease in the skin histamine content is useful as an index of the in vivo inhibitory effect of antiallergic drugs on the antigen-induced histamine release.
en-copyright= kn-copyright= en-aut-name=YokoyamaHiroshi en-aut-sei=Yokoyama en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShimamuraKazuhiro en-aut-sei=Shimamura en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SaekiKiyomi en-aut-sei=Saeki en-aut-mei=Kiyomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=passive cutaneous anaphylaxis(PCA) kn-keyword=passive cutaneous anaphylaxis(PCA) en-keyword=antiallergic drugs kn-keyword=antiallergic drugs en-keyword=histamine release kn-keyword=histamine release en-keyword=dye leakage kn-keyword=dye leakage END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=6 article-no= start-page=533 end-page=539 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Nonicteric liver damage with a gamma-glutamyl transpeptidase level of 5,609 units/l in a renal-transplant recipient receiving azathioprine. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 26-year-old male with renal allograft, who received immunosuppressive treatment with azathioprine, presented marked elevations of serum biliary tract enzymes, such as gamma-glutamyl transpeptidase (5,609 units/l) and alkaline phosphatase (60.5 Bessey-Lowry units), 14 months after transplantation. Two months later the patient became icteric; he died of respiratory failure 19 months after the renal allograft. Postmortem examination revealed intrahepatic cholestasis with minimal inflammatory cell infiltration, indicating drug hepatotoxicity.
en-copyright= kn-copyright= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ObataTakahiro en-aut-sei=Obata en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SakagamiKenichi en-aut-sei=Sakagami en-aut-mei=Kenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=renal transplantation kn-keyword=renal transplantation en-keyword= ?-GTP kn-keyword= ?-GTP en-keyword=intrahepatic cholestasis kn-keyword=intrahepatic cholestasis en-keyword=liver injury kn-keyword=liver injury en-keyword=azathioprine kn-keyword=azathioprine END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=6 article-no= start-page=541 end-page=556 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The present status of childhood cancer therapy in Korea. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We have studied the incidence pattern of childhood cancers in Korea. Although the incidence of many tumors in Korea is similar to that in other countries, the incidence of acute myelogenous leukemia, non-Hodgkin's lymphoma and hepatoma is greater in Korean children. Yonsei Cancer Center commenced a study of multi-modality treatment of childhood cancers in July 1974. The most striking improvement of survival rate was seen in patients with acute lymphocytic leukemia (50% at 5 years), Wilms' tumor (65% at 5 years), neuroblastoma (45% at 2 years), osteogenic sarcoma (55% at 2 years) and malignant histiocytosis (20% at 5 years). This study is an attempt to create a basic framework providing the best possible treatment of childhood cancer in Korea. The data obtained in Korea are briefly compared with those in Japan and the United States.
en-copyright= kn-copyright= en-aut-name=KimByung Soo en-aut-sei=Kim en-aut-mei=Byung Soo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Ronsei Cancer Center en-keyword=childwood cancer kn-keyword=childwood cancer en-keyword=multimodality treatment kn-keyword=multimodality treatment en-keyword=survival rate kn-keyword=survival rate END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=6 article-no= start-page=501 end-page=504 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Interaction between human lymphoblastoid interferon and chemotherapeutic agents in vitro. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The combined effect of human lymphoblastoid interferon (HLBI) and anticancer agents on the growth of MOLT-4 was studied in vitro. The interferon showed a strikingly synergistic interaction in combination with aclarubicin, cytosine arabinoside or prednisolone. It was moderately synergistic in combination with adriamycin or 5-fluorouracil and tended to show additive effects with daunorubicin or vincristine. In vitro studies of combination chemotherapy with interferon and anticancer agents should yield valuable information as to the best combination for man.
en-copyright= kn-copyright= en-aut-name=TakahashiIsao en-aut-sei=Takahashi en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OdaYasuhiro en-aut-sei=Oda en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=LaiMinyu en-aut-sei=Lai en-aut-mei=Minyu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FukumotoMitsuhiro en-aut-sei=Fukumoto en-aut-mei=Mitsuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NishimuraMasataka en-aut-sei=Nishimura en-aut-mei=Masataka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YorimitsuSeiichi en-aut-sei=Yorimitsu en-aut-mei=Seiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KitajimaKoichi en-aut-sei=Kitajima en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=human lymphoblastoid interferon kn-keyword=human lymphoblastoid interferon en-keyword=in vitro chemotherapy kn-keyword=in vitro chemotherapy en-keyword=interaction of anticancer agents kn-keyword=interaction of anticancer agents END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=6 article-no= start-page=565 end-page=567 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Two cases of constitutional unconjugated hyperbilirubinemia with marked retention of indocyanine green. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Two cases of chronic unconjugated hyperbilirubinemia and marked retention of indocyanine green (ICG) are described. Since bilirubin uridine diphosphate (UDP)-glucuronyl transferase activities were depressed in their liver, the patients seemed to have bilirubin metabolism similar to that in Gilbert's syndrome. However, the ICG fractional disappearance rates of the cases were rather low (0.018 and 0.019) compared to the rates reported for Gilbert's syndrome. These results suggest that the patients had a new metabolic disorder which results in constitutional unconjugated hyperbilirubinemia and ICG intolerance.
en-copyright= kn-copyright= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ItoshimaTatsuya en-aut-sei=Itoshima en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=yamamotoHiroshi en-aut-sei=yamamoto en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakahashiKenji en-aut-sei=Takahashi en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=Gilbert's syndrome kn-keyword=Gilbert's syndrome en-keyword=indocyanine green kn-keyword=indocyanine green en-keyword=bilirubin kn-keyword=bilirubin END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=6 article-no= start-page=505 end-page=510 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Detection of HLA-D-region-associated antigens on the surface of adult T-cell leukemia virus particles by immunoelectron microscopy. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To search for lymphocyte marker antigens on the surface of human T-cell leukemia virus (HTLV), an immunoelectron microscopic study was performed on a HTLV-producing human T-cell line, MT-2, using monoclonal antibodies, such as anti-Leu-1, -Leu-2b, -Leu-3a, -Leu-5, -Leu-10 and -HLA-DR and OKIal. The reactivity of each antibody with MT-2 cells was tested by the immunoperoxidase method at the light microscopic level. OKIal, anti-HLA-DR and -Leu-10 gave positive results. At the ultrastructural level, the surface of HTLV as well as the plasma membranes of MT-2 cells were labeled with ferritin by the monoclonal antibodies OKIal, anti-HLA-DR and -Leu-10, but not by anti-Leu-1 and -Leu-3a. These findings suggest that HLA-D region -associated antigens are common antigenic determinants shared by the surface of HTLV and the plasma membranes of MT-2 cells. These antigens on the virus surface are probably picked up selectively from the plasma membranes and may play an important role in the interaction of HTLV and target T-cells.
en-copyright= kn-copyright= en-aut-name=OhtsukiYuji en-aut-sei=Ohtsuki en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkagiTadaatsu en-aut-sei=Akagi en-aut-mei=Tadaatsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakedaIsao en-aut-sei=Takeda en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyoshiIsao en-aut-sei=Miyoshi en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Kochi Medical School en-keyword=adult T-cell leukemia kn-keyword=adult T-cell leukemia en-keyword=monoclonal antibodies kn-keyword=monoclonal antibodies en-keyword=HLA-D antigens kn-keyword=HLA-D antigens en-keyword=immunoelectron microscopy kn-keyword=immunoelectron microscopy en-keyword=human T-cell leukemia virus kn-keyword=human T-cell leukemia virus END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=6 article-no= start-page=511 end-page=524 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of carbon tetrachloride and azathioprine on diethylnitrosamine and N-2-fluorenylacetamide-induced hyperplastic liver nodule and hepatocellular carcinoma en-subtitle= kn-subtitle= en-abstract= kn-abstract=Effects of carbon tetrachloride (CCl4) and azathioprine (AZP) on the evolution of hyperplastic liver nodules and foci and hepatocellular carcinoma (HCC) were tested in short- and long-term in vivo experiments. In diethylnitrosamine (DEN)-treated rats, which were fed a N-2-fluorenylacetamide (FAA)-containing diet and additionally treated with repeated CCl4 injections, gamma-glutamyl transpeptidase (gamma-GTP)-positive hyperplastic nodules were markedly developed in the 8th week of the experiment. However, their number and area in liver sections were remarkably small in DEN-treated rats fed a diet containing both FAA and AZP. Increased area of gamma-GTP-positive foci was also observed in the 12th week in DEN-injected rats fed a choline-devoid died alone or treated with repeated doses of CCl4 alone. Hepatocellular carcinoma in DEN-injected rats treated with both FAA and CCl4 was first detected in the 21st week, and the incidence up to the 36th week was very high. However, no hepatocellular carcinoma developed in DEN-injected rats treated with both FAA and AZP. The increased activity of liver aniline hydroxylase observed 12 h after the administration of FAA, AZP or DEN alone was not observed when AZP was administered simultaneously with FAA to DEN-injected rats. The mechanisms of the effects of CCl4 and AZP on hepatocarcinogenesis are discussed with special reference to drug interaction.
en-copyright= kn-copyright= en-aut-name=SakataTatsuro en-aut-sei=Sakata en-aut-mei=Tatsuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma en-keyword=hyperplastic liver nodule kn-keyword=hyperplastic liver nodule en-keyword= ?-glitamyl transpeptidase kn-keyword= ?-glitamyl transpeptidase en-keyword=azathioprine kn-keyword=azathioprine en-keyword=carbon tetrachloride kn-keyword=carbon tetrachloride END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=6 article-no= start-page=493 end-page=499 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of sake and bourbon on liver histopathology and function in rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Sake or bourbon (8g ethanol/kg body weight) was intragastrically administered to rats for 12 days. An equal dose of ethanol in water or an isocaloric glucose solution was administered to control groups. Food was withheld, but water freely provided. Neither mortality nor liver and body weights were different between the alcohol-treated groups. Glutamic oxaloacetic transaminase and glutamic pyruvic transaminase were more elevated in the sake group than in the other groups. Additionally, liver fibrosis was more pronounced, and vacuole formation or steatosis was less in this group. These results suggest that sake is more fibrogenic. Some components other than ethanol, such as long-alkyl chain alcohols, may have been responsible for the differential histopathology.
en-copyright= kn-copyright= en-aut-name=KitadaiMasahiro en-aut-sei=Kitadai en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HattoriShuzo en-aut-sei=Hattori en-aut-mei=Shuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ItoshimaTatsuya en-aut-sei=Itoshima en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UkidaMinoru en-aut-sei=Ukida en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ItoToshio en-aut-sei=Ito en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OgawaHiromichi en-aut-sei=Ogawa en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MizutaniShigeki en-aut-sei=Mizutani en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KitaKeiji en-aut-sei=Kita en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TanakaRyoji en-aut-sei=Tanaka en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Okayama University en-keyword=alcoholic liver injury kn-keyword=alcoholic liver injury en-keyword=alcoholic beverages kn-keyword=alcoholic beverages en-keyword=alcoholic liver fibrosis kn-keyword=alcoholic liver fibrosis en-keyword=longchain alcohols kn-keyword=longchain alcohols en-keyword=rats kn-keyword=rats END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=6 article-no= start-page=557 end-page=563 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The evaluation of asymptomatic arterial occlusive disease of the legs using an exercise test. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The Doppler-derived ankle pressure index (API) is a useful indicator of the necessity for peripheral vascular reconstruction of the lower extremities. But the API at rest dose not reflect the functional capacity of leg circulation, especially in the early stage of disease. Therefore, an asymptomatic but hemodynamically significant lesion in one leg is sometimes missed by pressure measurement at rest when there is a severe lesion with symptoms in the other leg. In this study, the API not only at rest but also after exercise was measured in twenty normal subjects and thirty-two patients with angiographically proven arteriosclerosis obliterans. About 60% of the patients had unilateral symptoms, although they had significant disease bilaterally. The API after exercise proved to be more sensitive than the API at rest and may be useful in assessing asymptomatic legs of such patients and determining their surgical indication.
en-copyright= kn-copyright= en-aut-name=UsuiYoshiyuki en-aut-sei=Usui en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShimizuYasuhiro en-aut-sei=Shimizu en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UchidaHatsuzo en-aut-sei=Uchida en-aut-mei=Hatsuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TeramotoShigeru en-aut-sei=Teramoto en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=arteriosclerosis obliterans kn-keyword=arteriosclerosis obliterans en-keyword=asymptomatic legs kn-keyword=asymptomatic legs en-keyword=Doppler kn-keyword=Doppler en-keyword=exercise test kn-keyword=exercise test en-keyword=ankle pressure index kn-keyword=ankle pressure index END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=6 article-no= start-page=525 end-page=531 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Blood eosinophilia in bronchial asthma and its relationship to IgE-mediated reactions en-subtitle= kn-subtitle= en-abstract= kn-abstract=The correlation between blood eosinophilia and anti-IgE-mediated histamine release was investigated in 22 bronchial asthma patients with peripheral eosinophilia (over 8%). In the cases (Group A-1 and Group A-2) in which house dust was the specific antigen, significant histamine release from basophils was induced by anti-IgE and house dust. The result indicates a relationship between eosinophilia and the IgE-mediated mechanism of disease onset. In the cases (Group A-3) with RAST scores of 0+ and 1+ to house dust, the anti-IgE-induced histamine release varied from low to high percentages, and the participation of the IgE-mediated pathway was indicated in some cases. In the cases (Group B) with negative skin reactions, few patients had a family history of allergic disease. Their ages at onset were higher, and they demonstrated lower total IgE levels. These cases showed an extremely low percent of histamine release from basophils, which indicated the absence of a correlation between eosinophilia and IgE-mediated mechanisms.
en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitaniHikaru en-aut-sei=Kitani en-aut-mei=Hikaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TadaShinya en-aut-sei=Tada en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=blood eosinophilia kn-keyword=blood eosinophilia en-keyword=histamine release kn-keyword=histamine release en-keyword=IgE-mediated reaction kn-keyword=IgE-mediated reaction en-keyword=asthma kn-keyword=asthma END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=175 end-page=180 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Detection of serum blocking factors and antibodies to the albumin receptor on HBsAG particles in healthy persons and patients with liver diseases. en-subtitle= kn-subtitle= en-abstract= kn-abstract=An enzyme-linked immunosorbent assay (ELISA) for the detection of serum blocking factors (BF), or antibodies to the albumin receptor on HBsAg particles, was developed, and its clinical usefulness was examined in healthy persons and patients with liver diseases. Thirteen of 80 anti-HBs-positive female (16.3%) had BF, but all 25 male anti-HBs-positive, 41 female and 32 male anti-HBs-negative subjects were negative for BF. The activity of BF in BF-positive cases was not associated with the positive reciprocal hemagglutination titer of anti-HBs. For a neutralization test of BF, the BFs from 5 cases were absorbed with IgG-immunobeads. It was determined that these IgG-BFs were antibodies to the albumin receptors on HBsAg particles. No significance between positive-BF and abnormal S-GPT levels was recognized. These results suggest that the present test for the detection of BF, or anti-albumin receptor antibody, different from anti-HBs, might be useful for diagnosis of hepatitis B and as a marker for HB virus.
en-copyright= kn-copyright= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakahashiKenji en-aut-sei=Takahashi en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NaitoKunihiko en-aut-sei=Naito en-aut-mei=Kunihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Kumayama Municipal Hospital affil-num=4 en-affil= kn-affil=Okayama University en-keyword=HBV kn-keyword=HBV en-keyword=blocking factor to albumin receptor kn-keyword=blocking factor to albumin receptor en-keyword=antibody to albumin receptor kn-keyword=antibody to albumin receptor en-keyword=albumin receptor kn-keyword=albumin receptor en-keyword=ELISA kn-keyword=ELISA END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=147 end-page=158 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Prostacyclin and thromboxane in cerebral vasospasm: effects of prostacyclin on experimentally-induced cerebral vasospasm. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The basilar artery was exposed transclivally , and a vascular spasm was produced by topical application of a lysed erythrocyte solution. The maximum fall in the mean arterial blood pressure (MABP) after administering of 2 micrograms/ kgBW and 15 micrograms/ kgBW of PGI2, ranged from 35 to 45 mmHg and from 65 to 85 mmHg, respectively. The drop in MABP after an injection of papaverine hydrochloride (1.5 mg/ kgBW ) was between 30 and 40 mmHg. If MABP did not fall, the vessel diameter did not change. Although papaverine elicited marked dilation of both normal and spastic basilar arteries, PGI2 did not dilate normal basilar arteries and produced only a slight dilation of spastic basilar arteries. Subarachnoid hemorrhage (SAH) was simulated by an intracisternal injection of fresh autologous arterial blood 3 days prior to experimentation. Changes in regional cerebral blood flow (rCBF) were measured by the heat clearance method, before and after an intravenous administration of either PGI2 or papaverine hydrochloride. Changes in rCBF fell into 3 categories: Type A, no change; Type B, a change which varied with the arterial blood pressure, and Type C, an increase rCBF despite systemic hypotension. Type A or B was observed in 17 out of 19 cats with SAH in which PGI2 was administered intravenously, and Type C was observed in only 2 cats. Thirteen untreated control cats produced a Type A or B response in 12, and Type C response in only one cat. There were no significant differences between the control and SAH groups. When 15-hydroperoxy-5, 8, 11, 13-eicosatetraenoic acid (15-HPETE) was infused, the same results prevailed. Papaverine hydrochloride increased rCBF either transiently or continuously in all cats. These results suggest that PGI2 dilates extracranial rather than intracranial vessels regardless of the presence or absence of cerebral vasospasm.
en-copyright= kn-copyright= en-aut-name=YabunoNobuyoshi en-aut-sei=Yabuno en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=cerebral vasospasm kn-keyword=cerebral vasospasm en-keyword= thromboxane A2 kn-keyword= thromboxane A2 en-keyword= prostaglandin I2 kn-keyword= prostaglandin I2 en-keyword=papaverine kn-keyword=papaverine END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=159 end-page=168 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Diagnosis of. Hepatocellular Carcinoma in Patients with Liver Cirrhosis Using Liver Function Assays en-subtitle= kn-subtitle= en-abstract= kn-abstract=Sex, age and 21 routine liver function assays were analyzed by stepwise selection and the best-of-all-possible-combinations method to identify a small group of assays valuable in establishing which liver cirrhosis (LC) patients have a high risk of hepatocellular carcinoma (HCC), when alpha-fetoprotein (AFP) is not elevated. Data was obtained from 115 HCC and 122 LC patients on admission. Tumor size correlated with AFP (0.73), alkaline phosphatase (ALP, 0.47), leucine aminopeptidase (LAP, 0.42), lactic dehydrogenase (LDH, 0.42), and the glutamic oxaloacetic transaminase (GOT)/glutamic pyruvic transaminase (GPT) ratio (GOT/GPT, 0.41). The mean of the correct diagnosis rates (CDR) of HCC and LC utilizing AFP as the sole parameter (89%) was markedly higher than those of the other parameters. The best-of-all-possible-combinations method presented a more powerful combination than stepwise selection. The best combination of 7 parameters (LAP, GOT/GPT, choline esterase, one-hour erythrocyte sedimentation rate, age, albumin/globulin ratio, and total bilirubin) presented a mean CDR of 80%, HCC CDR of 77%, and false positive rate of 18%. LC patients statistically diagnosed as having HCC by these 7 parameters are proposed as high risk patients. Fourteen (78%) of 18 HCC patients who were AFP-negative were statistically diagnosed. This analysis can be applied to LC patients to distinguish those that should be followed closely by imaging diagnostic techniques.
en-copyright= kn-copyright= en-aut-name=ItoshimaTatsuya en-aut-sei=Itoshima en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KawaguchiKenji en-aut-sei=Kawaguchi en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UkidaMinoru en-aut-sei=Ukida en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ItoToshio en-aut-sei=Ito en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HattoriShuzo en-aut-sei=Hattori en-aut-mei=Shuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KitadaiMasahiro en-aut-sei=Kitadai en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OgawaHiromichi en-aut-sei=Ogawa en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MizutaniShigeki en-aut-sei=Mizutani en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KitaKeiji en-aut-sei=Kita en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TanakaRyoji en-aut-sei=Tanaka en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Okayama University affil-num=11 en-affil= kn-affil=Okayama University en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma en-keyword=liver cirrhosis kn-keyword=liver cirrhosis en-keyword=high risk hepatocellular carcinoma kn-keyword=high risk hepatocellular carcinoma en-keyword=liver function tests kn-keyword=liver function tests en-keyword=differentical diagnosis kn-keyword=differentical diagnosis END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=207 end-page=213 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Activation of NK activity and auto-reactive cytotoxicity after hepatectomy. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Spleen cells serially sampled from normal mice following partial hepatectomy were tested for antibody-dependent cellular cytotoxicity (ADCC) and natural killer (NK) activity. There was a marked augmentation of these activities of spleen cells from the hepatectomized animals, compared to cells from controls with a simple laparotomy. The augmentation of ADCC in the hepatectomized mice was largely attributable to the activity of T lymphocytes. When cultured with interleukin-2 (IL-2), the spleen cells from hepatectomized mice exhibited cytotoxicity to syngeneic lymphoblasts, which was found to be effected by T cells.
en-copyright= kn-copyright= en-aut-name=OnoMinoru en-aut-sei=Ono en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=antibody-dependent cellular cytotoxicity (ADCC) kn-keyword=antibody-dependent cellular cytotoxicity (ADCC) en-keyword=natural killer(NK) activity kn-keyword=natural killer(NK) activity en-keyword=auto-reactive cytotoxicity kn-keyword=auto-reactive cytotoxicity en-keyword= interleukin-2(IL-2) kn-keyword= interleukin-2(IL-2) en-keyword=hepatectomy kn-keyword=hepatectomy END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=101 end-page=115 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Inhibition of platelet aggregation by cepharanthine is accomplished during the early, membrane-related activation process. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cepharanthine, a biscoclaurine alkaloids which interact with biomembranes, has been found to inhibit platelet aggregation. The effects of this drug on morphological and physiochemical phenomena following collagen-induced platelet stimulation were investigated. In the presence of cepharanthine, stimulated platelets became spherical, but did not form pseudopoda , nor did they become aggregated. Physiochemical reactions such as accelerated oxygen consumption, release of membrane-bound Ca2+, release of Ca2+ into the extracellular medium and deporalization of the membrane potential were all inhibited by cepharanthine. Using D,L-dipalmitoyl phosphatidylcholine liposomes as the substrate, cepharanthine was shown to inhibit phospholipase A2 activity. These results suggest that the changes in the membrane following the interaction of collagen with its receptor are important for platelet activation. Cepharanthine may inhibits these membrane state changes, thus blocking all subsequent reactions.
en-copyright= kn-copyright= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=platelet aggregation kn-keyword=platelet aggregation en-keyword=cepharanthine kn-keyword=cepharanthine en-keyword=electron microscopy kn-keyword=electron microscopy en-keyword= phospholipase A2 kn-keyword= phospholipase A2 en-keyword=liposome kn-keyword=liposome END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=181 end-page=191 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Non-B hepatocellular carcinoma: influence of age, sex, alcohol, family clustering, blood transfusion and chronic liver disease. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In 144 cases of hepatocellular carcinoma (HCC), 166 cases of cirrhosis without HCC and 142 cases of chronic hepatitis, we examined HBsAg, anti-HBs and anti-HBc in sera and compared the following factors between hepatitis B virus marker-negative and -positive patients: age, sex, alcohol consumption, family clustering of liver diseases, and histories of blood transfusion and post-transfusion hepatitis. Results of this study demonstrated several distinct differences in clinical backgrounds between non-B (negative for HBsAg, anti-HBs and anti-HBc) and B (positive for HBsAg) patients with HCC. Non-B patients were significantly older, had a lower frequency of familial tendencies for liver diseases, and more frequently had cancers other than HCC in their families. Some of these differences were also observed between non-B and B patients with cirrhosis and chronic hepatitis. Among patients with chronic hepatitis, the non-B patients had received blood transfusion or had post-transfusion hepatitis more frequently than the B patients. However, this difference was not apparent in patients with liver cirrhosis or HCC, suggesting that progression of non-A, non-B post-transfusion hepatitis to cirrhosis and HCC may not be as frequent as progression to chronic hepatitis.
en-copyright= kn-copyright= en-aut-name=YamadaGotaro en-aut-sei=Yamada en-aut-mei=Gotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MizunoMotowo en-aut-sei=Mizuno en-aut-mei=Motowo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KinoyamaShingo en-aut-sei=Kinoyama en-aut-mei=Shingo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishiharaTakashi en-aut-sei=Nishihara en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OkushinHiroaki en-aut-sei=Okushin en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HyodoIchinosuke en-aut-sei=Hyodo en-aut-mei=Ichinosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SakamotoYuji en-aut-sei=Sakamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama Univeristy affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=non-B hepatocellular carcinoma kn-keyword=non-B hepatocellular carcinoma en-keyword=type non-A kn-keyword=type non-A en-keyword=non-B hepatitis kn-keyword=non-B hepatitis en-keyword=type B hepatitis kn-keyword=type B hepatitis en-keyword=post-transfusion hepatitis kn-keyword=post-transfusion hepatitis END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=117 end-page=124 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Production of a macromomycin (MCR)-monoclonal antibody conjugate and its biological activity. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Macromomycin (MCR), an unique membrane-reactive anticancer antibiotic, was incubated with murine monoclonal anti-HLA IgG1 antibody (H-1) in the presence of carbodiimide. The resulting mixture was fractionated with a Sephadex G-200 column. The first and second fractions were shown to contain MCR-(H-1) conjugate by the elution profile, as well as by the Sarcina lutea growth inhibition assay and Ouchterlony double-diffusion method. A membrane immunofluorescence test with anti-MCR and anti-mouse IgG antibodies demonstrated specific localization of MCR-(H-1) on the surface of HLA-bearing NALL -1 cells. MCR-(H-1) inhibited the growth of HLA-lacking NS-1 cells statistically less effectively than MCR alone (p less than 0.01). On the other hand, the conjugate and free MCR equally inhibited the growth and 3H-TdR incorporation of HLA-bearing NALL -1 cells. These results indicate that the antibody-bound MCR retained both MCR and antibody activities, and thus exerted antibody-targeting MCR cytotoxicity in vitro.
en-copyright= kn-copyright= en-aut-name=ManabeYuichi en-aut-sei=Manabe en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsubotaTeruhiko en-aut-sei=Tsubota en-aut-mei=Teruhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HarutaYuro en-aut-sei=Haruta en-aut-mei=Yuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KataokaKiyoshi en-aut-sei=Kataoka en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OkazakiMorihiro en-aut-sei=Okazaki en-aut-mei=Morihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HaisaShigeru en-aut-sei=Haisa en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NakamuraKunihiko en-aut-sei=Nakamura en-aut-mei=Kunihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=macromomycin(MCR) kn-keyword=macromomycin(MCR) en-keyword=anti-HLA monoclonal antibody kn-keyword=anti-HLA monoclonal antibody en-keyword=carbodiimide kn-keyword=carbodiimide END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=125 end-page=133 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Immunohistochemical demonstration of lysozyme in normal, reactive and neoplastic cells of the mononuclear phagocyte system. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Using the peroxidase antiperoxidase (PAP) method, lysozyme (LZM) was shown to exist in normal, reactive and neoplastic cells belonging to the mononuclear phagocyte system (MPS), but was not detected in histiocytosis X cells. Immunostaining for cytoplasmic LZM by the PAP method is useful for identification of mononuclear phagocytes and for diagnosis of the diseases in which these cells participate.
en-copyright= kn-copyright= en-aut-name=MotoiMakoto en-aut-sei=Motoi en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshinoTadashi en-aut-sei=Yoshino en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawabataKenji en-aut-sei=Kawabata en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IkeharaIkuko en-aut-sei=Ikehara en-aut-mei=Ikuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhsumiShozo en-aut-sei=Ohsumi en-aut-mei=Shozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OgawaKatsuo en-aut-sei=Ogawa en-aut-mei=Katsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=lysozyme kn-keyword=lysozyme en-keyword=PAP method kn-keyword=PAP method en-keyword=mononuclear phagocyte system kn-keyword=mononuclear phagocyte system END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=93 end-page=99 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Influence of liver injury on the catecholamine metabolism in rat brain. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The present study investigated the brain catecholamine metabolism of rats with liver injury induced either by malnutrition or with CCl4. In the malnutrition group, the plasma tyrosine concentration was low, while it showed a tendency to be high in the cerebral cortex. Dopamine concentrations were low in both the cerebral cortex and diencephalon. Norepinephrine concentrations were low in the cerebral cortex, striatum and diencephalon. Tyrosine hydroxylase activity was elevated while monoamine oxidase activity was decreased in the striatum. In the CCl4 group, tyrosine concentrations in the plasma and cerebral cortex did not change. The dopamine concentration in the cerebral cortex increased five days after, and the norepinephrine concentration in the diencephalon increased 24 h after the last administration of CCl4. These data suggest that catecholaminergic neurons in the brain may be substantially affected by liver injury. It may be considered that malnutrition disturbs brain development, particularly in young rats.
en-copyright= kn-copyright= en-aut-name=KaneyukiTakao en-aut-sei=Kaneyuki en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShohmoriToshikiyo en-aut-sei=Shohmori en-aut-mei=Toshikiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=brain kn-keyword=brain en-keyword=catecholamine kn-keyword=catecholamine en-keyword=malnutrition kn-keyword=malnutrition en-keyword=carbon tetrachloride kn-keyword=carbon tetrachloride en-keyword=liver injury kn-keyword=liver injury END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=193 end-page=205 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Quantitative psychometric testing and subclinical hepatic encephalopathy--comparative study between encephalopathic and non-encephalopathic patients with liver cirrhosis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Six quantitative psychometric tests were performed on 51 healthy subjects, 19 cirrhotic patients with subclinical hepatic encephalopathy (SHE), 32 cirrhotic patients without SHE, and 26 patients with other diseases. Strong correlations between age and the results of all the psychometric tests were observed in the healthy subjects (p less than 0.005). Sex and etiology of liver cirrhosis did not affect the test results. SHE patients, compared with non-SHE and health subjects, presented impairment in the ability to perform the tests, even in the absence of obvious clinical and electroencephalogram findings. In SHE patients, trailmaking test A (TMT A) yielded the highest frequency of abnormal values, 63%. TMT A results were abnormal in 80% of SHE patients with abnormal scores in other tests, and thus it seemed to be the most sensitive test. Liver function tests did not correlate with psychometric testing in any of the groups. Blood ammonia levels in SHE patients with abnormal TMT A scores correlated with TMT A scores (r = 0.752, p less than 0.01); this was not the case in SHE nor non-SHE patients with normal TMT A results. These data demonstrate the usefulness of psychometric tests in detecting SHE.
en-copyright= kn-copyright= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=psychometric test kn-keyword=psychometric test en-keyword=trailmaking test kn-keyword=trailmaking test en-keyword=reaction time kn-keyword=reaction time en-keyword=subclinical hepatic encephalopathy kn-keyword=subclinical hepatic encephalopathy END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=169 end-page=174 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A case of chronic pancreatitis successfully treated by endoscopic removal of protein plugs. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 56 years old male with chronic pancreatitis complained of intractable abdominal pain, anorexia, emaciation and peripheral edema. Medical treatment initiated only partial improvement in the general condition and hypoproteinemia. Endoscopic retrograde cholangiopancreatography revealed multiple filling defects in the dilated main pancreatic duct. Endoscopic aspiration of pure pancreatic juice yielded numerous protein plugs. The endoscopic removal of protein plugs from the pancreatic duct resulted in remarkable improvement in symptoms, laboratory findings and ERCP findings. We consider this procedure to be an important new treatment of chronic pancreatitis.
en-copyright= kn-copyright= en-aut-name=TsurumiTetsuya en-aut-sei=Tsurumi en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujiiYasuhisa en-aut-sei=Fujii en-aut-mei=Yasuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakedaMasahiko en-aut-sei=Takeda en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TanakaJuntaro en-aut-sei=Tanaka en-aut-mei=Juntaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HaradaHideo en-aut-sei=Harada en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OkaHiroo en-aut-sei=Oka en-aut-mei=Hiroo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Ehime Prefectural Central Hospital affil-num=2 en-affil= kn-affil=Ehime Prefectural Central Hospital affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=chronic pancreatitis kn-keyword=chronic pancreatitis en-keyword=protein plugs kn-keyword=protein plugs en-keyword=treatment of chronic pancreatitis kn-keyword=treatment of chronic pancreatitis en-keyword=pure pancreatic juice kn-keyword=pure pancreatic juice END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=2 article-no= start-page=135 end-page=146 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of induced hypertension on experimentally-induced cerebral arterial spasm. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ten adult cats were anesthetized and ventilated by respirator. After the basilar artery was exposed transclivally and visualized with an operative microscope, mean arterial blood pressure (MABP) was raised gradually by intravenous drip infusion of norepinephrine (5-20 micrograms/kg) or angiotensin-II-amide (0.3-1.0 micrograms/kg). At various blood pressures, microphotographs were taken. There was no appreciable change in vessel diameter at a MABP ranging from 78 to 191 mmHg. The blood pressure was allowed to return to the initial baseline level. Arterial spasm was produced by the topical application of 0.2 M calcium gluconate, which decreased the arterial diameter by 13 to 58 percent for more than 60 min. Blood pressure was increased again after the production of the arterial spasm. Significant increases in the diameter of the arteries were produced by the drug-induced hypertension at levels of MABP ranging from 82 to 192 mmHg. The maximum arterial dilations ranged from 123 to 208 percent of the untreated control. The degree of dilation of the arteries almost paralleled the rise in MABP. Norepinephrine and angiotensin-II had a similar effect on both the blood pressure and the arterial diameter. Induced hypertension would be expected to improve blood flow parameters in the case of spastic cerebral arteries.
en-copyright= kn-copyright= en-aut-name=ShimataKenji en-aut-sei=Shimata en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=cerebral arterial spasm kn-keyword=cerebral arterial spasm en-keyword=induced hypertension kn-keyword=induced hypertension en-keyword=norepinephrine kn-keyword=norepinephrine en-keyword=angiotensin-?-amide kn-keyword=angiotensin-?-amide END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=461 end-page=469 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Quantitative analysis of the production of heat-labile enterotoxin by enterotoxigenic Escherichia coli. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A modified method of passive immune hemolysis (PIH) was applied to the quantitative assay of heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli. The method enabled the measurement of 0.2 to 1.2 ng LT. The production of LT by enterotoxigenic E. coli under various conditions was analyzed using the modified method. LT production was intense during the logarithmic growth phase and decreased during the stationary growth phase. Lincomycin (50 to 100 micrograms/ml) affected cell growth slightly, but enhanced production of LT until the late-stationary growth phase. About 90% of the LT produced was retained in the cell, and the rest was excreted into the culture medium. The initial pH of the culture medium affected LT production. Alkaline pH enhanced LT production, though growth was depressed. Aeration enhanced both growth and LT production.
en-copyright= kn-copyright= en-aut-name=MinamiJunzabro en-aut-sei=Minami en-aut-mei=Junzabro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkabeAkinobu en-aut-sei=Okabe en-aut-mei=Akinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagataAkihide en-aut-sei=Nagata en-aut-mei=Akihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HayashiHideo en-aut-sei=Hayashi en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Kagawa Medical School affil-num=2 en-affil= kn-affil=Kagawa Medical School affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Kagawa Medical School en-keyword=heat-labile enterotoxin kn-keyword=heat-labile enterotoxin en-keyword=E kn-keyword=E en-keyword=coli kn-keyword=coli en-keyword=passive immune hemolysis kn-keyword=passive immune hemolysis END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=471 end-page=477 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Classification of asthma based on clinical symptoms: asthma type in relation to patient age and age at onset of disease. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Seventy-one cases of bronchial asthma were classified into three types: bronchospasm, bronchospasm-hypersecretion and bronchiolar obstruction types. The characteristics of each type were studied in relation to patient age and age at onset of the disease. In the 71 subjects studied, the most frequent type was the bronchospasm type followed by the bronchospasm-hypersecretion type and bronchiolar obstruction type. Intractable asthma was most frequently observed in the bronchiolar obstruction type and least in the bronchospasm type. Most of the patients under 50 years of age showed the bronchospasm type. The bronchospasm-hypersecretion type was characteristically accompanied by blood eosinophilia when the patient age was under 50 years. In the bronchospasm-hypersecretion type, the incidence of intractable asthma was high in patients under 50 years of age, but not remarkable in those over 50. A large proportion of the patients over 50 years of age were of the bronchiolar obstruction type. There was no difference in the incidence of intractable asthma between the two groups classified by age at onset.
en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShiotaYutaro en-aut-sei=Shiota en-aut-mei=Yutaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TadaShinya en-aut-sei=Tada en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=asthma classification kn-keyword=asthma classification en-keyword=clinical symptoms kn-keyword=clinical symptoms en-keyword=patient age kn-keyword=patient age en-keyword=age at onset kn-keyword=age at onset END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=423 end-page=437 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Degranulation effect of ferric nitrilotriacetate (Fe3+-NTA) on the pancreatic islet beta-cells: its acute toxic effect on glucose metabolism. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A single injection of ferric nitrilotriacetate (Fe3+-NTA) caused a transitory increase in plasma immunoreactive insulin (IRI) and plasma immunoreactive glucagon (IRG) in rats. They reached maximum levels at 2 days after injection and returned to the normal range at 10 days. At 2 days after Fe3+-NTA injection, blood glucose level was normal but the glucose tolerance test (GTT) was impaired. There was a further increase in plasma IRI level and IRG level was suppressed after glucose loading. At 10 days after Fe3+-NTA injection, glucose tolerance was normal and IRI also returned to the normal range. No degenerative changes were found on H.E.-stained rat pancreatic tissue sections after Fe3+-NTA injection. Histochemical staining, however, showed a reduction in beta-granules and heavy metals (Timm's granules) from islet cells in the central area of the rat pancreatic islet 1 to 3 days after injection of Fe3+-NTA. The fading remained in some islets even at 10 days after injection, but by then the beta-granule distribution was restored in most islet cells. The results indicate a single Fe3+-NTA injection induced transitory instability of the pancreatic islet beta-cell granules and the glucose intolerance with a hyperresponse of IRI.
en-copyright= kn-copyright= en-aut-name=YamanoiYasuhiro en-aut-sei=Yamanoi en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AwaiMichiyasu en-aut-sei=Awai en-aut-mei=Michiyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SenoSatimaru en-aut-sei=Seno en-aut-mei=Satimaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Shigei Medical Research Institute en-keyword=ferric nitrilotriacetate kn-keyword=ferric nitrilotriacetate en-keyword=glucose metabolism kn-keyword=glucose metabolism en-keyword=pancreatic islet cells kn-keyword=pancreatic islet cells en-keyword=pancreatic islet zinc kn-keyword=pancreatic islet zinc END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=483 end-page=486 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Production of monoclonal antibodies to human glomerular basement membrane. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Using the technique of somatic cell fusion, we produced monoclonal antibodies to collagenase-digested human glomerular basement membrane (GBM). Fourteen monoclonal antibodies which reacted with normal human kidney in indirect immunofluorescence (IIF) studies were produced. An analysis of the binding patterns indicated that the antigens recognized could be divided into six broad groups. Monoclonal antibody B3-H10 (Group 1) reacted with only GBM in a fine granular pattern. A5-B12 and B5-C2 (Group 2) reacted with GBM and peritubular capillary in a linear pattern. B2-A12 (Group 3) reacted with only epithelial cells. Al-C9 and A4-E2 (Group 4) showed a mesangial pattern in glomerulus and a lineal pattern in tubular basement membrane (TBM), Bowman's capsule and peritubular capillary. A1-E1, A1-E11, A2-E6, A3-B6, A4-F8 and B5-H2 (Group 5) recognized determinants common to GBM, TBM, Bowman's capsule and/or peritubular capillary. A3-F1 and B5-E10 (Group 6) reacted with TBM and Bowman's capsule. The staining pattern of B3-H10 (Group 1) was characteristic because it was not linear, but finely granular along the GBM. The staining pattern of B2-A12 (Group 3) was also characteristic because only epithelial cells were stained, and processes of epithelial cells were observed as fine fibrils. To the best of our knowledge, these two types of monoclonal antibodies have not been reported previously.
en-copyright= kn-copyright= en-aut-name=MinoYasuaki en-aut-sei=Mino en-aut-mei=Yasuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HirakawaShuzo en-aut-sei=Hirakawa en-aut-mei=Shuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=monoclonal antibodies kn-keyword=monoclonal antibodies en-keyword=human glomerular bacemant membrance kn-keyword=human glomerular bacemant membrance END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=439 end-page=446 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Suppressive influence of surgical stress on the graft-versus-host reaction in mice. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The influence of surgical stress on the local graft-versus-host reaction (GVHR) in F1 mice was studied. Skin incision 1 day prior to injection of parental spleen cells produced impairment of popliteal lymph node enlargement; however, this effect was not observed when GVHR was induced 3 and 5 days after operation. Strong GVHR suppressive activity of spleen cells was observed three hours after leg amputation before a decrease in thymus weight became evident. The GVHR suppressive activity declined by six hours later, but a second peak of 60% inhibition was observed after 24 h. This suppressive activity completely disappeared by treatment with anti-Thy 1.2 and complement. This shows that the GVHR is suppressed by surgical stress, and that this suppression is due to suppressor T lymphocytes.
en-copyright= kn-copyright= en-aut-name=FujiwaraRyoichi en-aut-sei=Fujiwara en-aut-mei=Ryoichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=surgical stress kn-keyword=surgical stress en-keyword=graft-versus-host reaction kn-keyword=graft-versus-host reaction en-keyword=suppressor T cells kn-keyword=suppressor T cells END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=447 end-page=452 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Brain tumors in hamsters induced by murine sarcoma virus (Moloney). en-subtitle= kn-subtitle= en-abstract= kn-abstract=<p>Murine sarcoma virus, CS-Moloney substrain, was inoculated intracranially into 2 litters of newborn Syrian hamsters within 24 h of birth. Seven of 12 hamsters which survived more than 30 days developed brain tumors in the cerebral cortex 104 to 153 days, 139 days on the average, after the virus inoculation. The tumors consisted of spindle-shaped, round or polygonal astrocytes which showed a positive reaction for glial fibrillary acidic protein by the immunoperoxidase method.</p>
en-copyright= kn-copyright= en-aut-name=MotoiMakoto en-aut-sei=Motoi en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OgawaKatsuo en-aut-sei=Ogawa en-aut-mei=Katsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=brain tumors kn-keyword=brain tumors en-keyword=hamsters kn-keyword=hamsters en-keyword=MSV-CS-Moloney kn-keyword=MSV-CS-Moloney END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=415 end-page=421 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Metabolism of L-cysteine in rats fed low and high protein diets. en-subtitle= kn-subtitle= en-abstract= kn-abstract=L-Cysteine (5.0 mmol per kg of body weight) was intraperitoneally injected into rats fed a 25% casein or 5% casein diet. Concentrations of acidic and neutral amino acids in various tissues were determined 2 h later. In the rats fed the 25% casein diet there was a tendency for tissue amino acid and glutathione levels to be slightly lower than controls. In the 5% casein diet group, however, concentrations of tissue amino acids and glutathione generally increased after L-cysteine administration. S-(2-Hydroxy-2-carboxyethylthio)cysteine (HCETC,3-mercaptolactate-cysteine disulfide), though in trace amounts, was detected in kidney and blood plasma in the 5% casein diet group. Increases in cysteine-glutathione disulfide in liver, kidney and erythrocytes in the 5% casein diet group were considerable. These results indicate that L-cysteine was rapidly metabolized in the 25% casein diet group through the oxidative pathway, while in the 5% casein diet group, in which liver cysteine dioxygenase activity is supposed to be quite low, the oxidative metabolism of L-cysteine decreased and part of the L-cysteine was metabolized through the transaminative pathway. Administration of 15.0 mmol L-cysteine per kg of body weight to rats fed the 25% casein diet resulted in an increase in cysteine-glutathione disulfide in liver, kidney and erythrocytes, and the appearance of HCETC in blood plasma.(ABSTRACT TRUNCATED AT 250 WORDS)
en-copyright= kn-copyright= en-aut-name=MikamiHaruhiko en-aut-sei=Mikami en-aut-mei=Haruhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HosakiYasuhiro en-aut-sei=Hosaki en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UbukaToshihiko en-aut-sei=Ubuka en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=cysteine kn-keyword=cysteine en-keyword=mercaptolactate-cysteine disulfide kn-keyword=mercaptolactate-cysteine disulfide en-keyword=cysteine-glutathione disulfide kn-keyword=cysteine-glutathione disulfide en-keyword=oxidative pathway kn-keyword=oxidative pathway en-keyword=transaminative pathway kn-keyword=transaminative pathway END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=487 end-page=491 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Depolarization-elicited accumulation of cyclic AMP in slices of rat cerebral cortex with a chronic epileptic focus en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ferrous chloride solution was injected unilaterally into the sensorimotor cortex of rats to induce a chronic epileptic focus. Accumulation of cyclic AMP elicited by depolarizing agents was determined in slices from different cortical areas of rats 30-60 days after the injection. In anterior cortical areas which include the sensorimotor cortex, the cyclic AMP accumulation elicited by ouabain or a high concentration of potassium ion was greater in electrographic spike activity on the dominant side than on the other. In posterior cortical areas, no difference in cyclic AMP accumulation was detected. The regional difference in the depolarization-elicited accumulation of cyclic AMP is discussed with regard to the process of epileptic focus.
en-copyright= kn-copyright= en-aut-name=HattoriYukio en-aut-sei=Hattori en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MoriwakiAkiyoshi en-aut-sei=Moriwaki en-aut-mei=Akiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YasuharaHiromichi en-aut-sei=Yasuhara en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishidaNobuyoshi en-aut-sei=Nishida en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HoriYasuo en-aut-sei=Hori en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=cyclic AMP kn-keyword=cyclic AMP en-keyword=depolarization kn-keyword=depolarization en-keyword=cortical slices kn-keyword=cortical slices en-keyword=ferrous chloride kn-keyword=ferrous chloride en-keyword=focal epilepsy kn-keyword=focal epilepsy en-keyword=rat kn-keyword=rat END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=479 end-page=482 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Prevention of methionine and ammonia-induced coma by intravenous infusion of a branched chain amino acid solution to rats with liver injury. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The prevention of hepatic encephalopathy by the intravenous infusion of a branched chain amino acid (BCAA)-enriched solution was investigated in methionine and ammonium acetate-treated rats whose liver was already injured with carbon tetrachloride. A BCAA-enriched solution protected the rats from entering a coma. The brain BCAA contents became higher, and the brain methionine and tyrosine levels and the ratio of glutamine to glutamic acid in the brain diminished after administering the BCAA-enriched solution.
en-copyright= kn-copyright= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HigashiToshihiro en-aut-sei=Higashi en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=branched chain amino acid kn-keyword=branched chain amino acid en-keyword=ammonia kn-keyword=ammonia en-keyword=methionine kn-keyword=methionine en-keyword=hepatic encephalopathy kn-keyword=hepatic encephalopathy END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=453 end-page=459 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Potentiation of carbon tetrachloride hepatotoxicity by beta-phenethyl alcohol. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Carbon tetrachloride (CCl4)-induced hepatotoxicity was potentiated by pretreatment with beta-phenethyl alcohol, abundantly present in sake. The injury was determined by serum GPT levels and histological examination. Similar results were observed in ethanol- and phenobarbital-pretreated rats. Acetaminophen-induced hepatotoxicity was not accentuated by beta-phenethyl alcohol or ethanol pretreatment. The activities of liver microsomal enzymes, such as cytochrome P-450, cytochrome b5 reductase, aniline hydroxylase and aminopyrine demethylase, were not altered in beta-phenethyl alcohol-pretreated rats. Thus, CCl4-induced hepatotoxicity potentiation by beta-phenethyl alcohol administration is postulated to be due to a mechanism other than increased free radical generation.
en-copyright= kn-copyright= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakatsukasaHarushige en-aut-sei=Nakatsukasa en-aut-mei=Harushige kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KobayashiMichio en-aut-sei=Kobayashi en-aut-mei=Michio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=?-phenethyl alcohol kn-keyword=?-phenethyl alcohol en-keyword=ethanol kn-keyword=ethanol en-keyword=phenobarbital kn-keyword=phenobarbital en-keyword=carbon tetrachloride kn-keyword=carbon tetrachloride en-keyword=acetaminophen kn-keyword=acetaminophen END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=305 end-page=313 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Vagina invasion by cervical carcinoma. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Seventy patients with cervical carcinoma who underwent radical hysterectomy and pelvic lymphadenectomy were evaluated to assess spread to the vagina. The overall vaginal invasion rate was 34.2% (24/70), with 36% (21/58) by squamous cell carcinoma, 25% (2/8) by adenocarcinoma and 25% (1/4) by adenosquamous carcinoma. A high vaginal invasion rate (45.7%) was noted in cases in which the cervical lesion was greater than 21 mm (p less than 0.05). Combined parametrial extention (45%) and combined lymph node metastasis (33.3%) were significantly higher in the vaginal invasion cases. The diagnostic accuracy of colposcopy and the Schiller test was 80% and 67% respectively. Histologically, the course of vaginal invasion by squamous cell carcinoma could be divided into : a) continuous invasion (16/21), b) incontinuous invasion via vessel permeation (3/21) and c) combined invasion (2/21). Both cases of vaginal invasion by adenocarcinoma were noted to spread by vessel permeation. Of the 7 cases of vessel permeation, colposcopic examination was positive in only one case. A high percentage of parametrial involvement and lymph node metastasis was noted in the vessel permeation type.
en-copyright= kn-copyright= en-aut-name=ChenNeng-Jing en-aut-sei=Chen en-aut-mei=Neng-Jing kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=cervical cancer kn-keyword=cervical cancer en-keyword=redical hysterectomy kn-keyword=redical hysterectomy en-keyword=vaginal invasion kn-keyword=vaginal invasion en-keyword=vessel permeation kn-keyword=vessel permeation END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=261 end-page=267 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synergistic interaction of corticotropin releasing factor and arginine vasopressin on adrenocorticotropin and cortisol secretion in Macaca fuscata. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Plasma immunoreactive CRF measured by radioimmunoassay decreased rapidly after intravenous injection of synthetic ovine corticotropin releasing factor (CRF) and showed a bi-exponential decay curve in five macaca fuscatas. Half lives of plasma immunoreactive CRF were 5.8 +/- 1.4 (Mean +/- SEM) min for the fast component and 38.3 +/- 2.4 min for the slow component. A bolus injection of 5 micrograms/kg CRF significantly increased the plasma cortisol level. CRF at 5 micrograms/kg induced a delayed response of ACTH and cortisol. Arginine vasopressin (AVP) at 0.5 micrograms/kg induced a slight increase in plasma ACTH and cortisol, but AVP at 0.1 micrograms/kg evoked no significant increase. When 0.5 micrograms/kg CRF and 0.1 micrograms/kg AVP were administered simultaneously, significant ACTH and cortisol responses occurred. The results indicate that CRF and AVP act synergistically to stimulate ACTH secretion in vivo.
en-copyright= kn-copyright= en-aut-name=HashimotoKozo en-aut-sei=Hashimoto en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurakamiKazuharu en-aut-sei=Murakami en-aut-mei=Kazuharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HattoriTeruhiko en-aut-sei=Hattori en-aut-mei=Teruhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=corticotropin releasing factor kn-keyword=corticotropin releasing factor en-keyword=arginine vasopressin kn-keyword=arginine vasopressin en-keyword=adrenocorticotropin kn-keyword=adrenocorticotropin en-keyword=cortisol kn-keyword=cortisol en-keyword=macaca fuscata kn-keyword=macaca fuscata END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=269 end-page=274 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=An adult case of chronic myelogenous leukemia with myeloblastic involvement of the central nervous system. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 31-year-old female with chronic myelogenous leukemia, who developed myeloblastic involvement of the central nervous system during acute myeloblastic transformation of the disease, was treated with methotrexate intrathecally. The therapy produced prompt clinical response and complete reversal of abnormal cerebrospinal fluid findings. However, the patient expired 10 months following the acute blastic crisis.
en-copyright= kn-copyright= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HattoriShyuzo en-aut-sei=Hattori en-aut-mei=Shyuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatoYasumasa en-aut-sei=Sato en-aut-mei=Yasumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitajimaKoichi en-aut-sei=Kitajima en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=chronic myelogenous leukemia kn-keyword=chronic myelogenous leukemia en-keyword=central nervous system leukemia kn-keyword=central nervous system leukemia en-keyword=methotrexate kn-keyword=methotrexate en-keyword=blastic crisis kn-keyword=blastic crisis END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=301 end-page=304 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Improvement in freezing phenomenon of Parkinson's disease after DL-threo-3, 4-dihydroxyphenylserine. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 77-year-old man with Parkinson's disease of long standing, under treatment with L-DOPA and benserazide, was administered DL-threo-3, 4-dihydroxyphenylserine (DL-threo-DOPS), a precursor of norepinephrine, for 10 days. With this administration the patient's freezing phenomenon was remarkably improved, and his dysarthria also showed improvement. When DL-threo-DOPS was suspended, the frozen gait returned on the third day to almost the former level, even though he continued to receive L-DOPA and benserazide. After administration of DL-threo-DOPS, the CSF level of 3-methoxy-4-hydroxyphenylglycol (MHPG), a major metabolite of norepinephrine, was 127.5% of the pretreatment level. These observations suggest that DL-threo-DOPS can pass through the blood-brain barrier and change to norepinephrine, and that DL-threo-DOPS may be beneficial in the treatment of the freezing phenomenon of Parkinson's disease.
en-copyright= kn-copyright= en-aut-name=OgawaNorio en-aut-sei=Ogawa en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KurodaHiroo en-aut-sei=Kuroda en-aut-mei=Hiroo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamamotoMitsutoshi en-aut-sei=Yamamoto en-aut-mei=Mitsutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NukinaItaru en-aut-sei=Nukina en-aut-mei=Itaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=DL-threo-DOPS kn-keyword=DL-threo-DOPS en-keyword=MHPG kn-keyword=MHPG en-keyword=freezing phenomenon kn-keyword=freezing phenomenon en-keyword=Parkinson's disease kn-keyword=Parkinson's disease END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=219 end-page=225 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Accelerated leucine decarboxylation in the rat brain in relation to increased blood ammonia levels during acute hepatic failure. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Leucine decarboxylation in rat brain was investigated during acute hepatic failure, induced by partial hepatectomy after carbon tetrachloride (CCl4) pretreatment of rats. These rats presented metabolic alkalosis, and had significantly higher levels of arterial blood and brain ammonia than control and CCl4-treated rats. Brain leucine decarboxylation was elevated in rats with hepatic failure. This alteration correlated with arterial blood ammonia concentrations, and probably with elevated brain ammonia levels, as brain ammonia levels were directly related to arterial blood ammonia.
en-copyright= kn-copyright= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=leucine kn-keyword=leucine en-keyword=decarboxylation kn-keyword=decarboxylation en-keyword=acute hepatic failure kn-keyword=acute hepatic failure en-keyword=ammonia kn-keyword=ammonia END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=281 end-page=290 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Clinical studies of resistant ascites in liver cirrhosis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Resistant ascites was studied in 34 patients with liver cirrhosis and ascites. The patients were initially divided into 3 groups on the basis of the weekly cumulative ascites retention curve: patients relieved of ascites within 3 weeks of admission, patients relieved between 4 and 12 weeks and patients with ascites persisting beyond 13 weeks. "Resistant ascites" was defined as "ascites persisting for more than 13 weeks after admission to the hospital". The patients were then reclassified into 3 groups : Group A being those patients relieved of ascites within 12 weeks, Group B being those with resistant ascites and group C being those who died within 12 weeks of admission. There were no differences in age and sex distribution, etiology of liver cirrhosis, past medical history or physical findings among the 3 groups. However, Group B had higher levels of serum creatinine and blood urea nitrogen than Group A on admission. Serum bilirubin was higher and serum albumin was lower in Group C than in Group B, which indicates that Group C had greater liver cell failure.
en-copyright= kn-copyright= en-aut-name=YuasaShiro en-aut-sei=Yuasa en-aut-mei=Shiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ItoshimaTatsuya en-aut-sei=Itoshima en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=liver cirrhosis kn-keyword=liver cirrhosis en-keyword=hepatic ascites kn-keyword=hepatic ascites en-keyword=resistant ascites kn-keyword=resistant ascites END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=227 end-page=237 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Differential sensitivity to aphidicolin of replicative DNA synthesis and ultraviolet-induced unscheduled DNA synthesis in vivo in mammalian cells. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In vivo in mammalian cells, ultraviolet-induced unscheduled DNA synthesis was less sensitive to aphidicolin than was replicative DNA synthesis. Replicative DNA synthesis in HeLa, HEp-2, WI-38 VA-13 and CV-1 cells was inhibited more than 97% by aphidicolin at 10 micrograms/ml, whereas aphidicolin inhibition of DNA synthesis in ultraviolet-irradiated cells varied between 30% and 90% depending on cell types and assay conditions. Aphidicolin inhibition of unscheduled DNA synthesis (UDS) in HeLa cells increased gradually with increasing aphidicolin concentration and reached approximately 90% at 100 micrograms/ml aphidicolin. A significant fraction of UDS in ultraviolet-irradiated HEp-2 cells was resistant to aphidicolin even at 300 micrograms/ml. Considered along with related information reported previously, the present results suggest that both aphidicolin-sensitive and insensitive DNA polymerases, DNA polymerase alpha and a non-alpha DNA polymerase (possibly DNA polymerase beta), are involved in in situ UDS in these ultraviolet-irradiated cells. Comparison of staphylococcal nuclease sensitivity between DNAs repaired in the presence and in the absence of aphidicolin in HEp-2 cells suggested that the involvement of DNA polymerase alpha in UDS favored DNA synthesis in the intranucleosomal region.
en-copyright= kn-copyright= en-aut-name=SekiShuji en-aut-sei=Seki en-aut-mei=Shuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HosogiNobuo en-aut-sei=Hosogi en-aut-mei=Nobuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=unscheduled DNA synthesis (mammalian cells) kn-keyword=unscheduled DNA synthesis (mammalian cells) en-keyword=DNA polymerase kn-keyword=DNA polymerase en-keyword=aphidicolin kn-keyword=aphidicolin en-keyword=ultraviolet irradiation kn-keyword=ultraviolet irradiation END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=239 end-page=250 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Prostacyclin and thromboxane in cerebral vasospasm II: Effects of thromboxane synthetase inhibitor (OKY-1581) on experimentally-induced cerebral vasospasm en-subtitle= kn-subtitle= en-abstract= kn-abstract=OKY-1581, a thromboxane A2 (TXA2) synthetase inhibitor, was administered to cats with normal and constricted basilar arteries. At a dose of 60mg/kg (i.v.), both normal and constricted vessels dilated, and the mean arterial blood pressure (MABP) fell from 55 to 75 mmHg. If MABP remained constant, vessel diameter did not change. Subarachnoid hemorrhage (SAH) was simulated by intracisternal injection of autologous arterial blood. Regional cerebral blood flow (rCBF) was assessed by the heat clearance and H2 clearance methods. The two methods presented similar response profiles. rCBF responses to intravenous OKY-1581 fell into 3 categories: A) no change in rCBF, B) decrease in rCBF related to MABP and C) increase in rCBF in the presence of hypotension. Types A and B were observed in 3 out of 10 control cats and 4 out of 14 SAH-induced cats, with Type C responses in the remainder. There was no significant difference between the groups. While the results do not support a major role for TXA2 in cerebral vasospasm pathogenesis, OKY-1581 may still be useful in the treatment of cerebral vasospasm, as it improves distal and deep circulation and inhibits platelet aggregation.
en-copyright= kn-copyright= en-aut-name=YabunoNobuyoshi en-aut-sei=Yabuno en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=cerebral vasospasm kn-keyword=cerebral vasospasm en-keyword= thromboxane A2 kn-keyword= thromboxane A2 en-keyword=OKY-1581 kn-keyword=OKY-1581 END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=315 end-page=320 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ceruloplasmin in human malignancies. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The ceruloplasmin concentration was determined in 145 cancer patients prior to and after treatment with different radiotherapeutic and chemotherapeutic regimes. The ceruloplasmin concentration was observed to be higher in patients with malignancies than in healthy controls. There was a positive correlation of the values with the clinical condition of the patients. The ceruloplasmin concentration was noted to stop increasing and subsequently fall in patients who responded to therapy, and, in contrast, to remain high or become higher in those who did not respond to therapy. The diagnostic and prognostic value of ceruloplasmin determination is discussed.
en-copyright= kn-copyright= en-aut-name=ChakrabortyPrabir Kishore en-aut-sei=Chakraborty en-aut-mei=Prabir Kishore kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=GhoshAparesh en-aut-sei=Ghosh en-aut-mei=Aparesh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ChowdhuryJ Roy en-aut-sei=Chowdhury en-aut-mei=J Roy kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Chittaranjan Natioinal Cancer Research Center affil-num=2 en-affil= kn-affil=Chittaranjan Natioinal Cancer Research Center affil-num=3 en-affil= kn-affil=Chittaranjan Natioinal Cancer Research Center en-keyword=ceruloplasmin kn-keyword=ceruloplasmin en-keyword=malignant tumors kn-keyword=malignant tumors en-keyword=prognosis kn-keyword=prognosis END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=215 end-page=218 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Identification and prognostic implications of tumor infiltrating lymphocytes--a review. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cancer patients who have many tumor infiltrating lymphocytes (TIL) tend to have better prognoses. A relationship between prognosis and TIL or regional lymph node response is present in several malignant diseases. TIL are mainly T lymphocytes, as ascertained by immunological methods. Results of studies on T-lymphocyte subsets comprising TIL using monoclonal antibodies (OKT series and Leu series) are summarized in this review.
en-copyright= kn-copyright= en-aut-name=MiwaHiroaki en-aut-sei=Miwa en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=tumor infiltrating lymphocytes(TIL) kn-keyword=tumor infiltrating lymphocytes(TIL) en-keyword=lymph node reaction kn-keyword=lymph node reaction en-keyword=cell mediated immunity kn-keyword=cell mediated immunity en-keyword=monoclonal antibodies kn-keyword=monoclonal antibodies en-keyword=local administration of immunomodulator kn-keyword=local administration of immunomodulator END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=291 end-page=299 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Clinical studies of hepatocellular carcinoma with liver cirrhosis and ascites. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A comparison was made of the clinical findings of 59 patients with liver cirrhosis (LC) accompanied with hepatocellular carcinoma (HCC) (of which 35 had ascites and 24 did not at the time of admission) and 164 patients with LC, but without HCC (of which 39 had ascites and 125 did not). HCC patients were older and more often had hepatomegaly, vascular spider and pleural effusion than LC patients. Ascites was more frequently observed in HCC than in LC patients when the serum albumin level and the indocyanine green disappearance rate were relatively well maintained and when peripheral edema was absent. There was no difference in the ascitic protein concentration between LC and HCC patients. Malignant cells were detected in ascites only in 14% of the HCC patients. These facts indicate the presence of ascites-inducing factors in HCC patients which have no direct relation to serum colloid osmotic pressure and effective hepatic blood flow. Almost all of the HCC patients with ascites (96%) died with ascites, whereas 54% of the LC patients with ascites recovered from the ascitic condition.
en-copyright= kn-copyright= en-aut-name=YuasaShiro en-aut-sei=Yuasa en-aut-mei=Shiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ItoshimaTatsuya en-aut-sei=Itoshima en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=liver cirrhosis kn-keyword=liver cirrhosis en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma en-keyword=ascites kn-keyword=ascites END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=275 end-page=280 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Histamine release from whole blood induced by anti-IgE: relationship to patient age, age at onset and serum IgE levels. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Anti-IgE-induced histamine release from basophils was examined in 46 asthmatic subjects using a whole blood method. Basophils from subjects less than 30 years old released more histamine than those from subjects aged 41 to 50. The age at onset of the disease also affected the reactivity of basophils to anti-IgE: basophils showed a high response in subjects whose age at onset was between 0 and 10 years, and low response in the subjects whose age at onset was between 41 and 50 years. There was a correlation between histamine release and serum IgE levels. However, individual dose-response curves of histamine release varied greatly in whom serum IgE levels were low.
en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitaniHikaru en-aut-sei=Kitani en-aut-mei=Hikaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TadaShinya en-aut-sei=Tada en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=histamine release kn-keyword=histamine release en-keyword=whole blood kn-keyword=whole blood en-keyword=patient age kn-keyword=patient age en-keyword=age a? onset kn-keyword=age a? onset en-keyword=serum IgE levels kn-keyword=serum IgE levels END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=251 end-page=260 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Preparation and Primary Culture of Liver Cells Isolated from Adult Rats by Dispase Perfusion en-subtitle= kn-subtitle= en-abstract= kn-abstract=The dispase perfusion technique was used to isolate liver cells from adult rats. The optimum conditions for obtaining many isolated liver cells with high viability were an enzyme concentration of 2000 U/ml, a pH of 7.5 and a perfusion time of 20 min. The population of isolated liver cells prepared with dispase consisted of 43.6% cells with diameters less than 20 micron and 56.4% cells with diameters above 20 micron. The isolated liver cells were cultured in basal culture medium either supplemented with or without dexamethasone (1 X 10(-5)M) and insulin (10 micrograms/ml). The addition of hormones to the culture medium improved the attachment efficiency of the isolated liver cells and delayed the disappearance of mature hepatocytes. Epithelial-like clear cells proliferated early in primary culture even in the presence of hormones. Therefore, functioning mature hepatocytes and proliferating epithelial-like clear cells coexisted well in the hormone-containing medium. Furthermore, the number of cultured cells reached a maximal level earlier in the presence of hormones than in the absence of hormones. The level of TAT activity in primary cultured cells was higher up to 3 days after inoculation in the presence of hormones than in their absence. No difference between G6Pase activity in primary cultured cells in the presence of hormones and that in the absence of hormones was found.
en-copyright= kn-copyright= en-aut-name=WahidSyarifuddin en-aut-sei=Wahid en-aut-mei=Syarifuddin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyazakiMasahiro en-aut-sei=Miyazaki en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatoJiro en-aut-sei=Sato en-aut-mei=Jiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=dispase-liver-perfusion kn-keyword=dispase-liver-perfusion en-keyword=sizu distribution kn-keyword=sizu distribution en-keyword=primary liver cell culture kn-keyword=primary liver cell culture en-keyword=grouwth pattern kn-keyword=grouwth pattern en-keyword=liver-specific functions kn-keyword=liver-specific functions END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=89 end-page=92 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Albumin receptors on HBsAG particles and subsequent sero-conversion designate HBeAG-positive patients among chronic active liver disease patients. en-subtitle= kn-subtitle= en-abstract= kn-abstract=An enzyme-linked immunosorbent assay (ELISA) for the purpose of detecting the albumin receptor on hepatitis B virus surface antigen (HBsAg) particles was developed. Patient sera with moderate to high receptor values demonstrated significant correlations with serum DNA-polymerase activity (p less than 0.005), but not with HBeAg titer. Within one year of the study of 47 HBeAg-positive patients, only in the group of 12 patients with the moderately high values and 9 with low values, did 2 (16.7%) and 6 cases (66.7%) sero-convert, respectively. These results suggest that the albumin receptor might be a useful marker of HBsAg-positive patients.
en-copyright= kn-copyright= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=albumin receptor kn-keyword=albumin receptor en-keyword=HBV kn-keyword=HBV en-keyword=HBeAg kn-keyword=HBeAg en-keyword=sero-conversion of HBeAg kn-keyword=sero-conversion of HBeAg en-keyword=chronic active hepatitis kn-keyword=chronic active hepatitis END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=41 end-page=48 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Progesterone receptor in the human uterine cervix. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A progesterone receptor (PR) in human uterine cervical nuclei was demonstrated by a nuclear exchange assay using a synthetic progestin, promegestone (R5020) as a radio-labeled ligand. Total exchange of previously bound progesterone with R5020 was achieved by incubation at 0 degree C for 3 h. A 0.6 M KCl solution was used to extract the nuclear PR in uterine cervical tissue, and the dextran coated charcoal (DCC) method was used to separate the free [3H] R5020 from the bound form. Scatchard plots of nuclear PR binding showed two components with dissociation constants of Kd = 2.3 X 10(-10) and 4.6 X 10(-9) M. Three histological regions of the uterine cervix was studied as to their nuclear PR contents throughout the menstrual cycle. In the follicular phase, the connective tissue (CT) had the highest PR concentration (658.9 fmole/mg DNA), followed by the columnar epithelium (CE) (253.6 fmole/mg DNA), and the squamous epithelium (SE) (184.7 fmole/mg DNA). In the luteal phase, there was no significant difference among the three regions. Comparing these phases of cycle revealed that the CT had higher PR contents in the follicular phase than in the luteal phase, but no such difference was found in the CE or SE. These three regions had the same Kd value in both phases.
en-copyright= kn-copyright= en-aut-name=LinTai-Tung en-aut-sei=Lin en-aut-mei=Tai-Tung kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=receptor kn-keyword=receptor en-keyword=progesterone kn-keyword=progesterone en-keyword=cervix uterus kn-keyword=cervix uterus END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=49 end-page=55 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antitumor effect of bacterial lipopolysaccharide (LPS) alone and in combination with lentinan on MH-134 tumors in C3H/He mice. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Using C3H/He mice, the antitumor effect of lipopolysaccharide (LPS) alone and in combination with Lentinan extracted from Lentinus edodes was studied. The influence of LPS on cellular immunity and the antitumor effect of the tumor necrosis factor (TNF) were also examined. LPS, which was administered into mice with tumor, induced hemorrhagic necrosis of the tumor within 48 h, demonstrating a high antitumor effect. When LPS was used in combination with Lentinan, the tumor growth was significantly inhibited as compared to that in the control mice. The combination of LPS and Lentinan prevented a decrease in the delayed type hypersensitivity in tumor bearing mice. Application of rabbit serum containing TNF resulted in hemorrhagic necrosis of the tumor within 48 h, as with LPS.
en-copyright= kn-copyright= en-aut-name=MoriyaNaoto en-aut-sei=Moriya en-aut-mei=Naoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NiwaHiroaki en-aut-sei=Niwa en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=antitumor effect kn-keyword=antitumor effect en-keyword=lipopolysaccharide kn-keyword=lipopolysaccharide en-keyword=lentinan kn-keyword=lentinan en-keyword=tumor necrosis factor kn-keyword=tumor necrosis factor END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=79 end-page=87 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Hemangioma of the buccal pad with phlebolithiasis: report of a case. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We report a case of a right buccal cavernous hemangioma with two phleboliths in a 23-year-old man. Consideration of the literature on the cases of hemangioma with phlebolithiasis in the maxillofacial and oral regions in Japan is given in this report.
en-copyright= kn-copyright= en-aut-name=IkegamiNobuyuki en-aut-sei=Ikegami en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishijimaKatsumi en-aut-sei=Nishijima en-aut-mei=Katsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=hemangioma kn-keyword=hemangioma en-keyword=phlebolithiasis kn-keyword=phlebolithiasis en-keyword=maxillofacial and oral regions kn-keyword=maxillofacial and oral regions END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=11 end-page=19 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Scanning electron microscopic analysis of Kupffer cell proliferation after zymosan administration. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Rat Kupffer cells were observed and counted under a scanning electron microscope after a single dose of zymosan. The cell number increased after 24 h and reached 2 times the control after 72 h. The percentage of cells presenting numerous microvilli increased from 24% to 86% over the same period. Carbon clearance, a measure of phagocytic activity, decreased temporarily, regained control values after 12 h, and attained a 4-fold elevation at 72 h. Phagocytic activity paralleled Kupffer cell number, but increased more relatively. This result probably reflected activation of resident Kupffer cells as noted by numerous thick microvilli.
en-copyright= kn-copyright= en-aut-name=KawaguchiKenji en-aut-sei=Kawaguchi en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=kupffer cell kn-keyword=kupffer cell en-keyword=zymosan kn-keyword=zymosan en-keyword=scanning electron microscopy kn-keyword=scanning electron microscopy en-keyword=carbon clearance kn-keyword=carbon clearance en-keyword=phagocytosis kn-keyword=phagocytosis END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=65 end-page=69 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Lack of uptake of indocyanine green and trypan blue by hepatocellular carcinoma. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Experimental hepatocellular carcinoma (HCC) in rats did not take up intravenously administered indocyanine green (ICG) and trypan blue, while surrounding tissue did. The lack of ICG uptake was also observed by peritoneoscopy in patients with HCC. The contrast between ICG-stained cirrhotic nodules and HCC tumors was intensified with infrared photography. Non-uptake of dyes by HCC cells may enable discrimination between tumors and normal cells.
en-copyright= kn-copyright= en-aut-name=ItoshimaTatsuya en-aut-sei=Itoshima en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ItoToshio en-aut-sei=Ito en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UkidaMinoru en-aut-sei=Ukida en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OgawaHiromichi en-aut-sei=Ogawa en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitadaiMasahiro en-aut-sei=Kitadai en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HattoriShunzo en-aut-sei=Hattori en-aut-mei=Shunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MizutaniShigeki en-aut-sei=Mizutani en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KitaKeiji en-aut-sei=Kita en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TanakaRyoji en-aut-sei=Tanaka en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KoideNorio en-aut-sei=Koide en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Okayama University affil-num=11 en-affil= kn-affil=Okayama University en-keyword=indocyanine green kn-keyword=indocyanine green en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma en-keyword=peritoneoscopy kn-keyword=peritoneoscopy en-keyword=trypan blue kn-keyword=trypan blue END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=21 end-page=27 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mechanism of rectal contraction mediated by sympathetic efferents from rectoanal pelvic afferents in guinea pigs. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In guinea pigs whose pelvic nerves were bilaterally sectioned, afferent stimulation of rectoanal branches of the pelvic nerve (PAS) could produce an intense contraction in the rectum similar to propulsive contractions elicited during defecation. The mechanism of this reflex was analyzed. Rectal contraction by PAS was abolished after transecting the spinal cord at T13 or sectioning the lumbar splanchnic nerves (LSN) or lumbar colonic nerves (LCN), but was unaffected by severing the intermesenteric and hypogastric nerves. Rectal contraction induced by PAS was abolished peripherally by atropine, guanethidine or yohimbine, while propranolol had no affect. Yohimbine antagonized the inhibitory effect of LSN or LCN stimulation on atropine-sensitive rectal contractions. It may, therefore, be concluded that PAS blocks the inhibition, by LCN efferents acting through alpha-adrenoreceptors, of cholinergic neurons in the myenteric plexus, thus facilitating recto-rectal propulsive contractions initiated by the defecation reflex.
en-copyright= kn-copyright= en-aut-name=NeyaToshiaki en-aut-sei=Neya en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakakiMiyako en-aut-sei=Takaki en-aut-mei=Miyako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakayamaSosogu en-aut-sei=Nakayama en-aut-mei=Sosogu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=intestine kn-keyword=intestine en-keyword=rectal motility kn-keyword=rectal motility en-keyword=enteric nerves kn-keyword=enteric nerves en-keyword=automic nerves kn-keyword=automic nerves en-keyword=defecation kn-keyword=defecation END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=29 end-page=34 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of cecectomy on feeding and defecation in rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effect of cecectomy on food intake and defecation was studied in Wistar rats fed ad libitum or under restriction. Food intake, fecal output, dry matter and water content of feces, and numbers of scybala increased significantly in both groups of cecectomized rats. The weight of scybala and their dry matter and water content also increased after cecectomy. Water intake did not change significantly. It was considered that changes in the parameters after cecectomy may have resulted from lowered digestion and enhancement of propulsive activity in the gastrointestinal tract.
en-copyright= kn-copyright= en-aut-name=ItanoNoriaki en-aut-sei=Itano en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=feeding kn-keyword=feeding en-keyword=defecation kn-keyword=defecation en-keyword=feces kn-keyword=feces en-keyword=cecum kn-keyword=cecum en-keyword=gastrointestinal motility kn-keyword=gastrointestinal motility END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=1 end-page=9 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Scanning electron microscopy of Ito's fat-storing cells in the rat liver. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The whole body including extended processes of Ito's fat-storing cells was observed by scanning electron microscopy in rat liver injured with lithocholic acid (LCA). Necrotic foci developed in the midlobular zone 48 h after LCA administration. Demonstration of Ito cell bodies around the foci was probably facilitated by easy detachment of hepatocytes from Ito cells. The body and the processes were located mainly between the sinusoidal endothelium and hepatocytes; sometimes they were between hepatocytes. Ito cells often were proximate to collagen fiber bundles and sometimes were attached to them. The cell body was flatly round or elliptic, 7 to 12 micron in diameter. Its surface was finely undulated with microvillous projections about 0.1 micron in length. Branching patterns of the processes resembled a fern-leaf mantling the sinusoidal endothelium. The trunks of the processes were about 2 micron in diameter and 20-30 micron in length. These processes tapered, branching into thinner processes, with the most peripheral being 0.1 micron in diameter. Ito cells and their branching processes likely strengthen sinusoidal walls and control blood flow in the sinusoids.
en-copyright= kn-copyright= en-aut-name=ItoToshio en-aut-sei=Ito en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ItoshimaTatsuya en-aut-sei=Itoshima en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UkidaMinoru en-aut-sei=Ukida en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TobeKazuo en-aut-sei=Tobe en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KiyotoshiShozo en-aut-sei=Kiyotoshi en-aut-mei=Shozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KawaguchiKeiji en-aut-sei=Kawaguchi en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OgawaHiromichi en-aut-sei=Ogawa en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=YamamotoHarumi en-aut-sei=Yamamoto en-aut-mei=Harumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=HattoriShuzo en-aut-sei=Hattori en-aut-mei=Shuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KitadaiMasahiro en-aut-sei=Kitadai en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=MizutaniShigeki en-aut-sei=Mizutani en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TsuchiyaTakahiro en-aut-sei=Tsuchiya en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=KitaKeiji en-aut-sei=Kita en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=TanakaRyoji en-aut-sei=Tanaka en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=NagasimaHideo en-aut-sei=Nagasima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= affil-num=1 en-affil= kn-affil=Okayma University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Okayama University affil-num=11 en-affil= kn-affil=Okayama University affil-num=12 en-affil= kn-affil=Okayama University affil-num=13 en-affil= kn-affil=Okayama University affil-num=14 en-affil= kn-affil=Okayama University affil-num=15 en-affil= kn-affil=Okayama University en-keyword=ito cell kn-keyword=ito cell en-keyword=fat-storing cell kn-keyword=fat-storing cell en-keyword=lithocholic acied kn-keyword=lithocholic acied en-keyword=liver cell necrosis kn-keyword=liver cell necrosis en-keyword=scanning electron microscopy kn-keyword=scanning electron microscopy END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=71 end-page=78 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Successful treatment with interferon of chicken pox in children with acute leukemia. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Childhood leukemia, especially acute lymphocytic leukemia, can now be completely cured by a multimodality approach in one out of every two patients. Since prolonged maintenance therapy with anti-cancer agents for three years is required for complete cure, a significant problem during this course of treatment is death due to secondary infection. Those with childhood leukemia receiving anti-cancer chemotherapy who became secondarily injected with chicken pox can now be treated successfully with interferon in the four cases reported here. Chicken pox was cured even while one of them was in relapse. Therefore, it can be said that a bright prospect, namely interferon, is on the horizon in the treatment of secondary viral diseases associated with acute leukemia.
en-copyright= kn-copyright= en-aut-name=KimByung Soo en-aut-sei=Kim en-aut-mei=Byung Soo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Ronsei University College of Medicine en-keyword=acute leukemia in children kn-keyword=acute leukemia in children en-keyword=interferon kn-keyword=interferon en-keyword=opportunistic infection kn-keyword=opportunistic infection END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=57 end-page=63 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of coenzyme Q10 on the survival time and lipid peroxidation of adriamycin (doxorubicin) treated mice. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effect of coenzyme Q10 (Co Q10) was examined on the survival time and lipid peroxidation of adriamycin (ADM)-treated ICR mice. Co Q10 showed a protective effect against a subacute toxicity in mice induced by two intraperitoneal administrations of ADM (15 mg/kg). The group treated orally with 10 mg/kg of Co Q10 showed the longest survival time of all the groups studied (16.81 +/- 10.29 days, mean +/- S.D.) and a significantly longer survival time (p less than 0.001) than the ADM-alone group (7.48 +/- 1.99 days). The inhibitory effect of Co Q10 on the plasma and tissue lipid peroxidation levels did not correlate with the effect of prolonging the survival time of mice. Co Q10 tended to inhibit rises in plasma and liver lipid peroxidation levels induced by ADM administration, but there was no statistically significant difference between treatments. There was a statistically significant different inhibitory effect in the kidney lipid peroxidation levels, but was not in those of the heart.
en-copyright= kn-copyright= en-aut-name=ShinozawaShinya en-aut-sei=Shinozawa en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EtowoKouhei en-aut-sei=Etowo en-aut-mei=Kouhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ArakiYasunori en-aut-sei=Araki en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=coenzymeQ10 kn-keyword=coenzymeQ10 en-keyword=adriamycin kn-keyword=adriamycin en-keyword=doxorubicin kn-keyword=doxorubicin en-keyword=lipid peroxidation kn-keyword=lipid peroxidation en-keyword=plasma and tissues kn-keyword=plasma and tissues en-keyword=toxicity kn-keyword=toxicity END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=1 article-no= start-page=35 end-page=40 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Clinical significance of eosinophilia in the diagnosis of halothane-induced liver injury. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Three patients with severe halothane-induced liver injury are described. All patients received halothane anesthesia twice within a short period. High fever and jaundice were noticed soon after the second operation. The prothrombin time was less than 40%, and eosinophilia was greater than 7% prior to these symptoms. Other causes of liver injury were excluded. Diagnostic criteria for halothane-induced liver injury are proposed.
en-copyright= kn-copyright= en-aut-name=FujiwaraMasachika en-aut-sei=Fujiwara en-aut-mei=Masachika kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatoYasumasa en-aut-sei=Sato en-aut-mei=Yasumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakatsukasaHarushige en-aut-sei=Nakatsukasa en-aut-mei=Harushige kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YamadaGotaro en-aut-sei=Yamada en-aut-mei=Gotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=halothane kn-keyword=halothane en-keyword=liver injury kn-keyword=liver injury en-keyword=eosinophilia kn-keyword=eosinophilia END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=213 end-page=219 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Blood microvascular organization of the nasal-associated lymphoid tissue of the guinea pig: a scanning electron microscopic study of corrosion casts. en-subtitle= kn-subtitle= en-abstract= kn-abstract=It has previously been confirmed that the guinea pig has aggregations of 10-20 lymphoid follicles at the junction of the nasal cavity and the nasopharyngeal duct. The vascular architecture of this nasal-associated lymphoid tissue (NALT) was studied by the corrosion cast/scanning electron microscope method. The NALT was supplied by branches of the inferior nasal artery. These afferent arterial branches gave off arterioles to the follicles and the interfollicular regions, where the arterioles ramified into capillaries. Some of these arterioles reached the subepithelial region to form a single-layer dense capillary network. The subepithelial capillaries gathered into short collecting venules, which in turn drained into high endothelial venules (HEV) in the interfollicular region. The HEV, which also receives tributaries from the follicular and interfollicular capillary plexuses, descended in the interfollicular regions and finally flowed into the efferent veins at the bottom of the NALT. Indentations impressed by high endothelial cells (HEC) were prominent on the surface of the HEV casts, and their frequency was larger in the upper course or segments than in the lower. This suggests that the incidence of HEC in the upper segments is higher than in the lower segments, and these findings are consistent with the hypothesis that some substances which are taken up into the subepithelial capillaries and transported to the venules induce differentiation and maintain of HEVs.
en-copyright= kn-copyright= en-aut-name=OkadaSatoko en-aut-sei=Okada en-aut-mei=Satoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishizakiKazunori en-aut-sei=Nishizaki en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MasudaYu en-aut-sei=Masuda en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=nasal-associated lymphoid tisse kn-keyword=nasal-associated lymphoid tisse en-keyword=vascular corrosion cast kn-keyword=vascular corrosion cast en-keyword=microvascular architecture kn-keyword=microvascular architecture en-keyword=high endothelial venule kn-keyword=high endothelial venule en-keyword=guinea pig kn-keyword=guinea pig END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=221 end-page=226 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Rotationplasty for patients with osteosarcoma around the knee joint. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The results of rotationplasty for patients with osteosarcoma around the knee joint are presented. After an average observation period of 13.3 months, there has been no local recurrence or metastasis. The ankle joints (the new knee joints) of the patients were able to support their body weight with an average range of motion of 75 degrees. All patients could walk well without crutches and without risk of the giving way phenomenon. The average rate of the functional evaluation according to the re-modified system by Enneking was 84.5% (range, 80.0-86.7%). No patient had psychological trouble in accepting the shortened and rotated extremity. The results show that rotationplasty is a useful reconstructive method for the treatment of osteosarcoma around the knee joint.
en-copyright= kn-copyright= en-aut-name=KawaiAkira en-aut-sei=Kawai en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HamadaMasanori en-aut-sei=Hamada en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SugiharaShinsuke en-aut-sei=Sugihara en-aut-mei=Shinsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HashizumeHiroyuki en-aut-sei=Hashizume en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NagashimaHiroaki en-aut-sei=Nagashima en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=rotationplasty kn-keyword=rotationplasty en-keyword=osteosacoma kn-keyword=osteosacoma en-keyword=reconstructive new knee joint kn-keyword=reconstructive new knee joint en-keyword=function kn-keyword=function END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=187 end-page=194 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of vasopressin V1- and V2-receptor stimulation on blood pressure in DOCA-salt hypertensive rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We recently reported that stimulation of the arginine vasopressin (AVP) V1-receptor enhanced the pressor response in spontaneously hypertensive rats (SHR). In the present study, we investigated acute changes in systolic blood pressure (SBP) and heart rate (HR) after intravenous injections of AVP, OPC-21268 (a V1-receptor antagonist), and OPC-31260 (a V2-receptor antagonist), in anesthetized DOCA-salt hypertensive rats (DOCA) and age-matched sham-operated Wistar rats (control) to determine whether the pressor effect is specific to SHR or is present in other hypertensive animal models. SBP increased significantly in DOCA rats 9 min after injection of AVP 5 ng/kg without a concomitant increase in HR. Neither OPC-21268 3mg/kg nor OPC-31260 3mg/kg caused significant changes in SBP or HR. SBP tended to increase when AVP was administered after injection of OPC-31260. HR increased significantly 15 min after the combined treatment with OPC-31260 and AVP in DOCA rats compared with control rats. SBP did not change significantly when AVP was administered after injection of OPC-21268 in DOCA or control rats, but HR decreased significantly from 1 to 4 min after injection of AVP in DOCA rats. Our results suggest that V1-receptor stimulation does not enhance the pressor response in the DOCA rat, which is a model of volume-dependent hypertension, suggesting that the AVP system, especially V1-receptor, is not as important in the development or maintenance of hypertension in DOCA rats as in SHR.
en-copyright= kn-copyright= en-aut-name=MimuraYukari en-aut-sei=Mimura en-aut-mei=Yukari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OguraToshio en-aut-sei=Ogura en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamauchiTakayoshi en-aut-sei=Yamauchi en-aut-mei=Takayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OtsukaFumio en-aut-sei=Otsuka en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OishiTetsuya en-aut-sei=Oishi en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HaradaKazushi en-aut-sei=Harada en-aut-mei=Kazushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HashimotoMasami en-aut-sei=Hashimoto en-aut-mei=Masami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=vasopressin kn-keyword=vasopressin en-keyword=DOCA-salt hypertensive rat kn-keyword=DOCA-salt hypertensive rat en-keyword=V1-and V2-receptor antagonist kn-keyword=V1-and V2-receptor antagonist en-keyword= spontaneously hypertesive rat(SHR) kn-keyword= spontaneously hypertesive rat(SHR) en-keyword=OPC-21268 kn-keyword=OPC-21268 END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=195 end-page=200 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Histopathological evaluation of gastric mucosal environments in peptic ulcer using the endoscopic 5-point gastric biopsy method. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Although a strong association has been established between chronic Helicobacter pylori infection and peptic ulcers, the role of H. pylori is not necessarily causative because there are many patients infected with H. pylori who do not develop peptic ulcer. Therefore, we studied the relationship between the gastric mucosal environment and the development of peptic ulcers. We examined 165 endoscopic biopsy specimens from the gastric mucosa of 33 patients with peptic ulcers using the 5-point gastric biopsy method. The follow-up biopsies done within 3 weeks were well correlated with the first biopsy samples. We also reviewed the clinicohistopathological findings of 2250 endoscopic biopsy specimens from 450 patients with active gastric and/or duodenal ulcers. Over 90% of the patients with duodenal ulcer, with or without gastric ulcer, had no fundic gland atrophy, and a high incidence of intestinal metaplasia and pyloric mucosal atrophy was found in the patients with gastric ulcer. These findings suggest that patients with concomitant active gastric and duodenal ulcers exhibit severe atrophic changes in the antral mucosa but not in the fundic mucosa.
en-copyright= kn-copyright= en-aut-name=KobayashiHisataka en-aut-sei=Kobayashi en-aut-mei=Hisataka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KajitaniYukio en-aut-sei=Kajitani en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MaekawaTakama en-aut-sei=Maekawa en-aut-mei=Takama kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ItohTsuyoshi en-aut-sei=Itoh en-aut-mei=Tsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MurataRumi en-aut-sei=Murata en-aut-mei=Rumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KanaokaMasaki en-aut-sei=Kanaoka en-aut-mei=Masaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NaokiMasao en-aut-sei=Naoki en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SatakeHiroshi en-aut-sei=Satake en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KogawaKochu en-aut-sei=Kogawa en-aut-mei=Kochu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KonishiJunji en-aut-sei=Konishi en-aut-mei=Junji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Kyoto National Hospital affil-num=2 en-affil= kn-affil=Kyoto National Hospital affil-num=3 en-affil= kn-affil=Kyoto National Hospital affil-num=4 en-affil= kn-affil=Kyoto National Hospital affil-num=5 en-affil= kn-affil=Kyoto University affil-num=6 en-affil= kn-affil=Kyoto National Hospital affil-num=7 en-affil= kn-affil=Kyoto National Hospital affil-num=8 en-affil= kn-affil=Kyoto National Hospital affil-num=9 en-affil= kn-affil=Kyoto National Hospital affil-num=10 en-affil= kn-affil=Kyoto University en-keyword=peptic ulcer kn-keyword=peptic ulcer en-keyword=endoscopy kn-keyword=endoscopy en-keyword=biopsy kn-keyword=biopsy en-keyword=mucosal atrophy kn-keyword=mucosal atrophy en-keyword=intestinal metaplasia kn-keyword=intestinal metaplasia END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=201 end-page=204 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of exposure to cigarette smoke on intestinal propulsion in rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of acute exposure to cigarette smoke and systemic administration of nicotine on intestinal propulsion were investigated in rats. The propulsive activity was measured as migration of charcoal powder in the intestine. This activity was suppressed by acute exposure (10 min) to cigarette smoke and by nicotine (0.5 mg/kg x 2, s.c.) administration. This intestinal suppression was more marked in the rats given nicotine than in those exposed to cigarette smoke, whereas the plasma concentrations of nicotine in both rats were similar. These results suggest that acute exposure to cigarette smoke and nicotine administration delay gastric emptying and/or suppress intestinal propulsion, and that some components other than nicotine contained in cigarette smoke may attenuate the suppression of intestinal propulsion induced by nicotine.
en-copyright= kn-copyright= en-aut-name=FurunoKatsushi en-aut-sei=Furuno en-aut-mei=Katsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkazakiMasatoshi en-aut-sei=Okazaki en-aut-mei=Masatoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=EtoKohei en-aut-sei=Eto en-aut-mei=Kohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univresity affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=cigarette smoke kn-keyword=cigarette smoke en-keyword=nicotine kn-keyword=nicotine en-keyword=intestinal propulsion kn-keyword=intestinal propulsion END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=179 end-page=185 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Possible Neuroprotective Therapy for Parkinson's Disease en-subtitle= kn-subtitle= en-abstract= kn-abstract=Neuroprotective therapy for Parkinson's disease (PD) is a treatment intended to prevent or reduce neuronal degeneration. Since clinical studies to evaluate such an effect would be prolonged, the choice of agents for use as possible neuroprotective therapy is based on the results of in vitro and animal experiments. Free radicals are currently regarded as the most important factor in the progression of PD. One current possible neuroprotective therapy is reduction of levodopa dose, since levodopa is a source of free radical formation. Dopamine (DA) metabolism inhibition, and administration of the DA agonist bromocriptine that eliminates hydroxyl free radicals have neuroprotective effects experimentally. The other candidates for neuroprotective agents are still under development. However, those whose clinical use is permitted should be considered for use, since patients with long-standing PD cannot wait until the neuroprotective efficacy of these agents is confirmed by clinical study.
en-copyright= kn-copyright= en-aut-name=OgawaNorio en-aut-sei=Ogawa en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=free radical kn-keyword=free radical en-keyword=scavengers kn-keyword=scavengers en-keyword=antioxidants kn-keyword=antioxidants en-keyword=antiexcitotoxic kn-keyword=antiexcitotoxic en-keyword=neurotrophic factors kn-keyword=neurotrophic factors END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=227 end-page=230 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Expression of Fas antigen and Bcl-2 protein in hepatocellular carcinoma. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Fas antigen (ag) is a cell surface protein known to trigger apoptosis in a variety of cells upon specific antibody binding. On the other hand, Bcl-2 protein, an oncogene product located at the mitochondrial inner surface, prolongs cell survival by blocking apoptosis. In this study we examined the expression of Fas ag and bcl-2 protein in 17 cases of hepatocellular carcinoma (HCC) to determine their role on HCC. By flow cytometric analysis, mean (SD) value of the expression of Fas ag on hepatocytes derived from normal liver, diseased liver (chronic hepatitis or liver cirrhosis) and HCC was 5.8 (4.7)%, 10.3 (6.9)%, and 24.0 (18.2)%, respectively. Fas ag expression on hepatoma cells was significantly greater than normal and diseased liver cells. The expression of Bcl-2 protein in normal liver, diseased liver and HCC was 4.3 (8.5)%, 0.8 (2.5)% and 2.1 (3.4)%, respectively, and the difference was not significant. These results suggest that induction of apoptosis may be a possible therapy against HCC.
en-copyright= kn-copyright= en-aut-name=HamazakiKeisuke en-aut-sei=Hamazaki en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=GochiAkira en-aut-sei=Gochi en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsubaraNagahide en-aut-sei=Matsubara en-aut-mei=Nagahide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoriMazanobu en-aut-sei=Mori en-aut-mei=Mazanobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=apoptosis kn-keyword=apoptosis en-keyword=Fas antigen kn-keyword=Fas antigen en-keyword=Bcl-2 kn-keyword=Bcl-2 en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=205 end-page=211 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Lipid profiles of Helicobacter pylori and Helicobacter mustelae grown in serum-supplemented and serum-free media. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Many of Helicobacter species have been found to have novel cholesteryl glucosides (CGs). To study the biosynthetic mechanism of CGs, the lipid profiles of H. pylori and H. mustelae grown in serum-supplemented and cholesterol-restricted serum-free media were investigated. In contrast to the serum-supplemented state, helicobacters had less CGs in the serum-free state; a trace amount of CGs and no CG was detected in H. pylori and H. mustelae, respectively. The proportion of total and individual phospholipid also showed significant alteration. Unknown lipids which did not contain phosphate and sugar were detected in the serum-free state, but not in the serum-supplemented state. The CGs were found to be distributed mainly in the membrane fractions, and one of the unknown lipids was found exclusively in the cytosol fraction. Based on these data, it is apparent that the CGs of helicobacters are synthesized by de novo uptake of cholesterol from the media. The unknown lipids detected in the serum-free state may be storage lipids, appearing in response to depletion of nutrients, especially cholesterol, or other factors in the media.
en-copyright= kn-copyright= en-aut-name=HaqueMahmudul en-aut-sei=Haque en-aut-mei=Mahmudul kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiraiYoshikazu en-aut-sei=Hirai en-aut-mei=Yoshikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YokotaKenji en-aut-sei=Yokota en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OgumaKeiji en-aut-sei=Oguma en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=Helicobacter kn-keyword=Helicobacter en-keyword=steryl glycoside kn-keyword=steryl glycoside en-keyword=cholesteryl glucoside kn-keyword=cholesteryl glucoside END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=6 article-no= start-page=309 end-page=312 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of varicella zoster virus or herpes simplex virus type I infection in vitro on response of human peripheral blood mononuclear cells to phytohemagglutinin. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Examination was made of the in vitro response of human peripheral blood mononuclear cells (PBNMCs) to phytohemagglutinin (PHA) following treatment with varicella zoster virus (VZV) or herpes simplex virus type 1 (HSV 1). Cell proliferation was determined by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide. The response to PHA was depressed in all cases by virus infection of PBMNCs prior to PHA treatment. When the infection with the viruses was after PHA treatment, PHA response differed. For VZV infection, the response increased in four out of six samples, but was reduced in the other two. The response to PHA was depressed in all six samples by HSV 1 infection.
en-copyright= kn-copyright= en-aut-name=HoriuchiYasuhiro en-aut-sei=Horiuchi en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkunoToshiomi en-aut-sei=Okuno en-aut-mei=Toshiomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamanishiKoichi en-aut-sei=Yamanishi en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Kitasato University affil-num=2 en-affil= kn-affil=Osaka Univeristy affil-num=3 en-affil= kn-affil=Osaka University en-keyword=virus infection kn-keyword=virus infection en-keyword=nonspecific mitogen kn-keyword=nonspecific mitogen en-keyword=immune response kn-keyword=immune response END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=6 article-no= start-page=313 end-page=315 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Lipid peroxidation in hepatocellular carcinoma. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this study, we measured free radicals and thiobarbituric acid-reactive substances (TBARS) in hepatocellular carcinoma and in non-cancerous liver parenchyma. There was a higher concentration of free radicals in malignant tissue than in non-cancerous tissue. In contrast, the level of TBARS was significantly (P < 0.01) lower than non-cancerous liver parenchyma. These paradoxical results suggested that antioxidative enzyme activity and/or inhibition of lipid peroxidation were higher in hepatocellular carcinoma.
en-copyright= kn-copyright= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HamazakiKeisuke en-aut-sei=Hamazaki en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsubaraNagahide en-aut-sei=Matsubara en-aut-mei=Nagahide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HiramatsuMidori en-aut-sei=Hiramatsu en-aut-mei=Midori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MoriAkitane en-aut-sei=Mori en-aut-mei=Akitane kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma en-keyword=free radicals kn-keyword=free radicals en-keyword=lipid peroxidation kn-keyword=lipid peroxidation END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=6 article-no= start-page=281 end-page=286 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ICRF-193 modifies etoposide-induced apoptosis in thymocytes. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Etoposide (VP-16), one of the topoisomerase II (TopoII) inhibitors, interferes with TopoII by inducing the formation of and stabilizing a cleavable enzyme-DNA complex. VP-16 has been demonstrated to induce apoptosis in murine thymocytes. To clarify the mechanism of action of VP-16, we examined the in vitro effect of a non-cleavable-complex-forming type TopoII inhibitor, ICRF-193 which inhibits the DNA strand breakage induced by VP-16, on murine thymocytes in which apoptosis had been induced with VP-16. DNA fragmentation is characteristic of apoptosis. In the early stages, ICRF-193 decreased DNA fragmentation induced by VP-16, although this inhibitory effect decreased in the later. These data suggest that TopoII inhibitors induce apoptosis in murine thymocytes in two ways: with DNA-strand breaks in the early stage or without them. ICRF-193 itself induced apoptosis in murine thymocytes. The time course of DNA fragmentation caused by ICRF-193 was different from that of VP-16.
en-copyright= kn-copyright= en-aut-name=TanimotoChikako en-aut-sei=Tanimoto en-aut-mei=Chikako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HirakawaShuzo en-aut-sei=Hirakawa en-aut-mei=Shuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawasakiHidetaka en-aut-sei=Kawasaki en-aut-mei=Hidetaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HayakawaNobuhiko en-aut-sei=Hayakawa en-aut-mei=Nobuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=etoposide kn-keyword=etoposide en-keyword=thymocyte kn-keyword=thymocyte en-keyword=apoptosis kn-keyword=apoptosis en-keyword=ICRF-193 kn-keyword=ICRF-193 en-keyword=cleavable complex kn-keyword=cleavable complex END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=6 article-no= start-page=287 end-page=294 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Localization of the glycosaminoglycans in the synovial tissues from osteoarthritic knees. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Localization of the glycosaminoglycans (GAG) was examined in the synovial membranes of patients with osteoarthritis under light microscopy using a fine cationic colloidal iron staining method combined with enzymatic digestion. Our staining method was very useful for demonstrating the difference in the localization of GAG in regions of the inflammatory site in the osteoarthritic synovial membrane. Hyaluronic acid was mainly located in connective tissues in the surface intercellular and perivascular spaces, chondroitin sulfate A/C in the highly fibrous part of and connective tissue around blood vessels, dermatan sulfate (chondroitin sulfate B) in the subsurface interstitium and vascular endothelial cells and heparan sulfate in part of vascular endothelial cells. No keratan sulfate was detected. GAG is reported to have an important role in cell movement, adherence and aggregation in the inflammatory sites. These findings should be useful for understanding the role of GAG in physiological and pathologic processes of secondary synovitis.
en-copyright= kn-copyright= en-aut-name=NishidaKeiichiro en-aut-sei=Nishida en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TodaKazukiyo en-aut-sei=Toda en-aut-mei=Kazukiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univresity affil-num=4 en-affil= kn-affil=Okayama University en-keyword=glycosaminoglycan kn-keyword=glycosaminoglycan en-keyword=synovial tissue kn-keyword=synovial tissue en-keyword=osteoarthritis kn-keyword=osteoarthritis en-keyword=fine cationic colloidal iron kn-keyword=fine cationic colloidal iron END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=6 article-no= start-page=295 end-page=300 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Relationship between retention of the posterior cruciate ligament and postoperative flexion in total knee arthroplasty. en-subtitle= kn-subtitle= en-abstract= kn-abstract=This study was conducted to retrospectively analyzed the outcome of 192 total knee arthroplasties in 132 patients with rheumatoid arthritis (118 women, 14 men). The Okayama Mark II prosthesis, which requires the posterior cruciate ligament (PCL) to be resected, was used in 83 knees (group I), the Mark II prosthesis, which allows the PCL to be retained, was used in 68 knees (group II), and the new Okayama PCL-R prosthesis, which also allows the PCL to be retained, was used in 41 (group III). According to the Japanese Orthopaedic Association knee scoring system, the clinical outcome of groups I, II and III at 1 year after the operation were 64.9, 71.2 and 72.3 points, respectively, and the average flexion angles in each group at 1 year were 78.4, 92.6 and 101.3 degrees. Postoperative flexion in groups III was significantly greater than in groups I and II. These results suggest that postoperative flexion is greater when the posterior cruciate ligament is retained.
en-copyright= kn-copyright= en-aut-name=YokoyamaYoshiki en-aut-sei=Yokoyama en-aut-mei=Yoshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OhtaYusuke en-aut-sei=Ohta en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HayashiTakashi en-aut-sei=Hayashi en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KouraHiroshi en-aut-sei=Koura en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=total knee arthroplasty kn-keyword=total knee arthroplasty en-keyword=posterior cruciate ligament kn-keyword=posterior cruciate ligament en-keyword=PCL resection kn-keyword=PCL resection en-keyword=PCL retention kn-keyword=PCL retention END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=6 article-no= start-page=275 end-page=279 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antioxidant activity of Chinese ant extract preparations. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Chinese ant extract preparations (CAEP) are a Chinese traditional medicine which is mainly used as a health food or drink for the treatment of rheumatism, rheumatoid arthritis, chronic hepatitis, sexual hypofunction, and antiaging in China. The effects on free radicals were examined by electron spin resonance spectrometry using the spin trapping agent 5.5-dimethyl-1-pyrroline-1-oxide (DMPO). Superoxide radicals (3.35 x 10(15) spins/ml) were quenched 50% by the extract at 0.5 mg/ml. The CAEP extract at 0.7 mg/ml inhibited 50% of hydroxyl radicals (52.0 x 10(15) spins/ml) generated by the Fenton reaction. Against DPPH radical, the scavenging action of CAEP was observed at 1.8 mg/ml of the extract and 50% of the DPPH radicals (8.14 x 10(15) spins/ml) were quenched. In vitro tests showed that CAEP inhibited the production of thiobarbituric acid-reactive substances, an index of lipid peroxidation, in rat brain homogenate.
en-copyright= kn-copyright= en-aut-name=ZhaoYi en-aut-sei=Zhao en-aut-mei=Yi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WangXiaoyan en-aut-sei=Wang en-aut-mei=Xiaoyan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawaiMotoko en-aut-sei=Kawai en-aut-mei=Motoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=LiuJiankang en-aut-sei=Liu en-aut-mei=Jiankang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=LiuMiao en-aut-sei=Liu en-aut-mei=Miao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MoriAkitane en-aut-sei=Mori en-aut-mei=Akitane kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Guangxi College of TCM affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=free radical scavenger kn-keyword=free radical scavenger en-keyword=antioxidant kn-keyword=antioxidant en-keyword=hydroxyl radical kn-keyword=hydroxyl radical en-keyword=superoxide radical kn-keyword=superoxide radical en-keyword=lipid peroxidation kn-keyword=lipid peroxidation END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=6 article-no= start-page=301 end-page=308 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Laparoscopic cholecystectomy: a multicenter study of 17 hospitals. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A study of 1,254 laparoscopic cholecystectomies performed at 17 hospitals affiliated with the Liver, Gallbladder, and Pancreas Research Group of the First Department of Surgery at Okayama University was undertaken to assess the current status and safety of this procedure. The data for 336 patients, comprising the initial 20 laparoscopic cholecystectomies performed at each institution, were compared with the data from the remaining 918 patients. Comparison of the two groups revealed the following: 1. the rates of intraoperative conversion to open cholecystectomy were 11.3% and 5.1% (P < 0.05), 2. the complication rates were 5.7% and 3.4%, and 3. the rates of bile duct injury were 2.4% and 1.1%, respectively. Compared with the first group, the bile duct injuries resulting from a lack of experience decreased in the second group, however, the incidence of these injuries occurring during avulsion of the gallbladder in difficult cases increased. These results suggest that the experience acquired during the initial 20 laparoscopic cholecystectomies led to a reduction in the rate of intraoperative conversion to open cholecystectomy, but it did not reduce the rate of complications, and that the risk of bile duct injury was always present.
en-copyright= kn-copyright= en-aut-name=TsugeHiromu en-aut-sei=Tsuge en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HamazakiKeisuke en-aut-sei=Hamazaki en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoriMasanobu en-aut-sei=Mori en-aut-mei=Masanobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MimuraHisashi en-aut-sei=Mimura en-aut-mei=Hisashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=laparoscopic cholecystectomy kn-keyword=laparoscopic cholecystectomy en-keyword=cholelithiasis kn-keyword=cholelithiasis en-keyword=bile duct injury kn-keyword=bile duct injury END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=2 article-no= start-page=97 end-page=106 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Sex differential in life expectancy in Japan and Scotland: age and causes of death. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The contribution of age groups and causes of death to the sex difference in life expectancy (SDLE) at birth in Japan and Scotland was estimated for the period 1965-1990. The purpose was to determine the particular age groups and causes of death responsible for the opposite trend of SDLE in the two countries. SDLE has been widening and narrowing in Japan and Scotland, respectively. The availability of complete and reliable data for these two developed countries facilitated the study. A method of decomposing the total SDLE into age and cause of death components was employed. About 40-60% contribution to SDLE was observed for ages after 65 years. Marked increase in the contribution of the 75+ age group and marked decrease in the contribution of ages 45-64 for Japan and Scotland, respectively, had a major effect on the widening and narrowing of SDLE in the two countries, respectively. The contribution of diseases of the circulatory system was the maximum until 1980 in Japan (< or = 1.8 years or 33.6%; cerebrovascular disease alone < or = 23.4%) and until 1990 in Scotland (< or = 3.1 years or 47.0%; ischemic heart disease alone < or = 42.0%). In Japan, the contribution of malignancy had a marked increased from 0.7 year (12.3%) to 2.0 years (32.6%), particularly for the trachea, bronchus and lung, while there was only a small increase in Scotland from 1.0 year (16.6%) to 1.2 years (19.8%) with an increase in the negative contribution of female breast malignancy. In Japan, the contribution of diseases of the respiratory system increased considerably from 0.5 year (8.5%) to 1.1 years (18.1%) while it decreased in Scotland from 1.0 year (16.5%) to 0.6 year (10.7%). About 60-75% of SDLE is due to the above three groups of causes of death. Malignancy and diseases of the respiratory system had a persistently increased contribution in Japan with resultant widening of SDLE by 0.9 year. Diseases of the circulatory system have always had a high contribution. On the contrary, in Scotland the contribution of diseases of the circulatory system and malignancy was practically unchanged and diseases of the respiratory system had a decrease with a consequent narrowing of SDLE by 0.4 year. Further epidemiological study is necessary to detect and analyze in detail the internal gradients (environmental and genetic-biological) of major contributor diseases to SDLE in Japan and Scotland.
en-copyright= kn-copyright= en-aut-name=QureshiMahboob H en-aut-sei=Qureshi en-aut-mei=Mahboob H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KatohTanekatsu en-aut-sei=Katoh en-aut-mei=Tanekatsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=LibuchiYasuo en-aut-sei=Libuchi en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=University of Ryukyus affil-num=2 en-affil= kn-affil=University of Ryukyus affil-num=3 en-affil= kn-affil=University of Ryukyus en-keyword=contribution to sex difference in life expectancy kn-keyword=contribution to sex difference in life expectancy en-keyword=japan and Scotland kn-keyword=japan and Scotland en-keyword=age kn-keyword=age en-keyword=cause of death kn-keyword=cause of death END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=2 article-no= start-page=107 end-page=112 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Training intensities for aerobic exercise determined on untrained healthy men. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The purpose of this study was to determine the aerobic training intensity from the maximal and submaximal running exercise in 21 untrained adult men. To accomplish this, we evaluated the relationship between physiological (oxygen intake and heart rate) and physical parameters (running speed) of training intensity, and determined the training intensity at the submaximal exercise. Oxygen intake and heart rate were measured by a treadmill test. The maximal oxygen intake (VO2 max), and the aerobic threshold (AerT) and anaerobic threshold (AT) were measured to determine respiratory gas exchange. Running capacity was measured by a 12-min running and treadmill test. For the maximal exercise, there was a significant correlation (r = 0.88, P < 0.01) between VO2 max and 12-min running distance (speed). In addition, the oxygen intake and heart rate at AerT and AT in the submaximal exercise were linearly correlated with running speed. Three levels of training intensity at the submaximal exercise were termed: light, moderate, and heavy. Since AerT was the lower limit intensity and AT was the upper limit, we took the middle of their values as the moderate intensity. The end point for the determination of the training intensity at the submaximal exercise was estimated to be 85% VO2 max and 180 beats.min-1.
en-copyright= kn-copyright= en-aut-name=MiuraMochiyoshi en-aut-sei=Miura en-aut-mei=Mochiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KohdaShigeki en-aut-sei=Kohda en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MinoYoshio en-aut-sei=Mino en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AoyamaHideyasu en-aut-sei=Aoyama en-aut-mei=Hideyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Joetsu University of Education affil-num=2 en-affil= kn-affil=Kochi Medical School affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=aerobic exercise kn-keyword=aerobic exercise en-keyword=training intensity kn-keyword=training intensity en-keyword=aerobic threshold kn-keyword=aerobic threshold en-keyword=anaerobic threshold kn-keyword=anaerobic threshold en-keyword=submaximal exercise kn-keyword=submaximal exercise END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=2 article-no= start-page=69 end-page=73 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Detection of reverse transcriptase activity by enzyme-linked immunosorbent assay in human immunodeficiency virus type 1. en-subtitle= kn-subtitle= en-abstract= kn-abstract=An enzyme-linked immunosorbent assay (ELISA) using biotin-labelled oligo-dT primer and digoxigenin (Dig)-dUTP was designed to measure the reverse transcriptase (RT) activity of human immunodeficiency virus type 1 (HIV-1). The ELISA system involves the selective detection step of a newly synthesized cDNA by two specific bindings, biotin-streptavidin binding and alkaline phosphatase (AP)-conjugated anti-Dig-Dig binding, and the enzymatic amplification step to increase coloring generated by AP. This method was used to measure the activity of RT in the culture supernatants of peripheral leukocytes obtained from four anti-HIV-1-positive persons cocultivated with those from four anti-HIV-1-negative persons. RT activity was detected in all of four anti-HIV-1-positive culture supernatants but not in those cultivated with anti-HIV-1-negative supernatants alone. Thus, our improved ELISA for detection of HIV-1 appears to be sensitive enough and useful for routine laboratory work. This non-radioactive method will also be useful for detecting other retroviruses and for screening of RT inhibitors.
en-copyright= kn-copyright= en-aut-name=TomitaNoriko en-aut-sei=Tomita en-aut-mei=Noriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyaharaMasayuki en-aut-sei=Miyahara en-aut-mei=Masayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatohHiromasa en-aut-sei=Satoh en-aut-mei=Hiromasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SuzukiKazuo en-aut-sei=Suzuki en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitajimaKoichi en-aut-sei=Kitajima en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MiyamotoKanji en-aut-sei=Miyamoto en-aut-mei=Kanji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama Red Cross Blood Center affil-num=2 en-affil= kn-affil=Okayama Red Cross Blood Center affil-num=3 en-affil= kn-affil=Okayama Red Cross Blood Center affil-num=4 en-affil= kn-affil=Biomedical Research Center affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Biomedical Research Center en-keyword=human immunodeficiency virus kn-keyword=human immunodeficiency virus en-keyword=reverse transcriptase kn-keyword=reverse transcriptase en-keyword=ELISA kn-keyword=ELISA END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=2 article-no= start-page=81 end-page=89 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Current status of antimicrobial susceptibility in MRSA isolates typed by coagulase and phage typing in Okinawa. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The incidence of nosocomial infections with methicillin-resistant Staphylococcus aureus is of great concern in Japan and the developed world as a whole. Simple typing techniques like coagulase and phage typing are quick and useful for monitoring and evaluating these organisms. In view of this, the current status of antimicrobial susceptibility in Staphylococcus aureus (S. aureus) isolates in Okinawa typed by coagulase and phage typing was studied. Of 508 isolates, methicillin-resistant S. aureus (MRSA) comprised 54.3% (minimum inhibitory concentration (MIC) > or = 16 micrograms/ml). Coagulase type II and phage group III were the most prevalent, comprising 65.2% and 38%, respectively. These were followed by phage non-typable group and coagulase type III with 36.6% and 12.7%, respectively. Compared to a previous study conducted in 1989, there has been an increase of about 17% in the MRSA isolation rate with a concomitant increase of about 11% in the coagulase type II MRSA isolation rate and a decrease of about 27% in the isolation rate of coagulase type III MRSA. Using a panel of 16 antibiotics, coagulase type II MRSA were resistant to all except Arbekacin and Vancomycin. Arbekacin and Vancomycin were the sole antibiotics to which resistance was not expressed by any of the isolates. With regard to the methicillin-sensitive S. aureus (MSSA), coagulase type III and phase group III were the most prevalent, comprising 25.9% and 32.3%, respectively.
en-copyright= kn-copyright= en-aut-name=LotsuDela Kwasi en-aut-sei=Lotsu en-aut-mei=Dela Kwasi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ImamuraTeisuke en-aut-sei=Imamura en-aut-mei=Teisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakamineFusae en-aut-sei=Takamine en-aut-mei=Fusae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=University of the Ryukyus affil-num=2 en-affil= kn-affil=University of the Ryukyus affil-num=3 en-affil= kn-affil=University of the Ryukyus en-keyword=coagulase type kn-keyword=coagulase type en-keyword=phage type kn-keyword=phage type en-keyword=antibiotic kn-keyword=antibiotic en-keyword=MRSA kn-keyword=MRSA en-keyword=MSSA kn-keyword=MSSA END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=2 article-no= start-page=75 end-page=79 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of Picibanil (OK 432) on the Scavenging Effect of Free Radicals Produced during Liver Regeneration in the Rat en-subtitle= kn-subtitle= en-abstract= kn-abstract=We administered a biological response modifier Picibanil (OK-432), attenuated Streptococcus pyogenes, via the dorsal vein of the penis after 70% hepatectomy in rats, and clarified the scavenging effect of Picibanil on free radicals generated in the regenerating liver. A group of 5 rats was intravenously administered with 25 KE/kg of OK-432 after hepatectomy, while the control group was given saline after hepatectomy. Serum levels of aspartate aminotransferase and alanine aminotransferase and the value of thiobarbituric acid-reactive substances in serum and hepatic tissue after hepatectomy were serially measured, and these values were significantly lower in Picibanil treated animals than in control animals. Free radical production in the regenerating liver was also measured by electron spin resonance spectrometry, and OK-432 injection significantly reduced free radical production. These results suggested that OK-432 reduced hepatocellular damage in regenerating liver by inhibiting lipid peroxidation.
en-copyright= kn-copyright= en-aut-name=OkamotoKo en-aut-sei=Okamoto en-aut-mei=Ko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HamazakiKeisuke en-aut-sei=Hamazaki en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MoriAkitane en-aut-sei=Mori en-aut-mei=Akitane kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=Picibanil kn-keyword=Picibanil en-keyword=free radicals kn-keyword=free radicals en-keyword=hepatectomy kn-keyword=hepatectomy en-keyword=liver damage kn-keyword=liver damage END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=2 article-no= start-page=61 end-page=68 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Changes in dopamine D2 and GluR-1 glutamate receptor mRNAs in the rat brain after treatment with phencyclidine. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In situ hybridization of slide-mounted brain sections from rats subjected to acute and chronic phencyclidine treatment was carried out using synthetic oligonucleotides complementary to dopamine D2-receptor and non-N-methyl-D-aspartate (NMDA) glutamate-receptor-subunit (GluR-1) mRNAs. There was no significant difference in either the D2-receptor or the GluR-1 mRNA levels in any brain region of the acute phencyclidine (10 mg/kg)-treated and control groups. However, chronic administration of phencyclidine (10 mg/kg/day, 14 days) significantly decreased the dopamine D2-receptor mRNA level in the caudate-putamen (by 27%, P < 0.01) and significantly increased the GluR-1 mRNA level in the prefrontal cortex (by 29%, P < 0.001). These results suggest that the chronic pharmaco-behavioral effects of phencyclidine may involve expression of both dopamine- and non-NMDA glutamate-receptor mRNAs.
en-copyright= kn-copyright= en-aut-name=TomitaHiroaki en-aut-sei=Tomita en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HikijiMitsuru en-aut-sei=Hikiji en-aut-mei=Mitsuru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujiwaraYutaka en-aut-sei=Fujiwara en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AkiyamaKazufumi en-aut-sei=Akiyama en-aut-mei=Kazufumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OtsukiSaburo en-aut-sei=Otsuki en-aut-mei=Saburo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=dopamone D2 receptor kn-keyword=dopamone D2 receptor en-keyword=GluR-1 glutamate reseptor kn-keyword=GluR-1 glutamate reseptor en-keyword=mRNA kn-keyword=mRNA en-keyword=phencyclidine kn-keyword=phencyclidine en-keyword=in situ hybridization kn-keyword=in situ hybridization END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=2 article-no= start-page=113 end-page=115 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Surgical treatment for Crohn's disease. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The medical records of 16 consecutive patients with Crohn's disease surgically treated in our department from 1978 to 1993 were retrospectively reviewed. The indication for surgery was obstructive symptoms due to Crohn's strictures that were unresponsive to conservative therapy. The types of operations performed were classified into five categories. Nine patients (56.3%) had small bowel resection only, 4 (25.0%) underwent an ileocolonic resection, 1 (6.3%) had a total colectomy, 1 (6.3%) had Mile's operation and 1 (6.3%) had subtotal gastrectomy with gastrojejunostomy and antral mucosectomy. Of these 16 patients, 13 (81.3%) had resection with a single anastomosis and strictureplasty was concomitantly performed in only 2 cases (12.5%). Crohn's disease recurred in 3 patients (18.8%), 1 of whom required a second operation.
en-copyright= kn-copyright= en-aut-name=PerdomoJose Antonio en-aut-sei=Perdomo en-aut-mei=Jose Antonio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HizutaAkio en-aut-sei=Hizuta en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MizunoMotowo en-aut-sei=Mizuno en-aut-mei=Motowo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakagawaHitoshi en-aut-sei=Nakagawa en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=Crohn's disease kn-keyword=Crohn's disease en-keyword=surgical resection kn-keyword=surgical resection en-keyword=strictureplasty kn-keyword=strictureplasty en-keyword=recurrence kn-keyword=recurrence END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=2 article-no= start-page=91 end-page=95 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Measurement of hepatic blood flow using 111In colloid. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The reduced hepatic blood flow calculated from hepatic scintigram with 198Au colloid was elucidated as the primary responsible factor for postoperative hepatic insufficiency. However 198Au colloid is no longer in use because of the high levels of radiation. Although 99mTc-phytate behaves similarly to 198Au on imaging, there were discrepancies between the hepatic blood flow index (KL) value and the severity of cirrhosis determined by laboratory data or by histology. In the measurement of hepatic blood flow using a radioactive colloid, factors like organ distribution, stability and uniformity of the colloid particles influence the values. In the present study, a 111In colloid was prepared and administered to rats to investigate the usefulness: as much as 95.4 (0.8) [Mean (+/- SD)]% of the colloid accumulated in the liver at pH 6.8. The distribution of particle diameter was within a relatively narrow range with the peak at 0.2 to 0.4 microns. Moreover, the KL values were not affected by condition of the reticuloendothelial system. The values showed a significant correlation with the measurements of the hepatic tissue blood flow obtained by the hydrogen gas clearance method (gamma = 0.83, P < 0.001). Thus, the 111In colloid can be clinically used as a substitute for 198Au colloid in the preoperative examination for estimation of the limit of resection.
en-copyright= kn-copyright= en-aut-name=SakumotoShuichi en-aut-sei=Sakumoto en-aut-mei=Shuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HamazakiKeisuke en-aut-sei=Hamazaki en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MimuraHisashi en-aut-sei=Mimura en-aut-mei=Hisashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=111in colloid kn-keyword=111in colloid en-keyword=hepatic functional reserve kn-keyword=hepatic functional reserve en-keyword=hepatic blood flow kn-keyword=hepatic blood flow END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=261 end-page=265 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Diagnostic sensitivity of motor nerve conduction studies in ulnar neuropathy at the elbow. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Seventy-six patients with ulnar neuropathy at the elbow were divided into 3 classes (Grades I, II, and III) according to their clinical features and the maximal motor nerve conduction velocity (MCV), and the amplitude ratios at the across-elbow segment were retrospectively analyzed. To determine the criteria for abnormality, a control study was conducted on 150 healthy volunteers ranging in age from 20 to 89 years (6 age groups). The normal value for MCV could be set for two age groups: those under 60 and those over 60 years old. The 95% confidence limit was 54m/s for the former and 50m/s for the latter. There was no statistically significant difference in the amplitude ratio among the age groups. The confidence limit was set uniformly at 0.82 (above elbow/below elbow). An abnormality in either MCV or the amplitude ratio was found in 66.7% of Grade I (recent and mild symptoms), 89.7% of Grade II (persistent symptoms), and 100% of Grade III cases (marked intrinsic muscle atrophy). Evaluation using the combination of MCV and the amplitude ratio, considering the age-related normal value, appeared to be useful in establishing a differential diagnosis of ulnar neuropathy at the elbow.
en-copyright= kn-copyright= en-aut-name=YokotaTadaaki en-aut-sei=Yokota en-aut-mei=Tadaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HamadaMasanori en-aut-sei=Hamada en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagashimaHiroaki en-aut-sei=Nagashima en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University Hospital en-keyword=entrapment neuropathy kn-keyword=entrapment neuropathy en-keyword=ulnar nerve kn-keyword=ulnar nerve en-keyword=electrodiagnosis kn-keyword=electrodiagnosis en-keyword=M-wave kn-keyword=M-wave END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=267 end-page=270 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Relationship between body mass index (BMI) and changes in plasma total and HDL-cholesterol levels during treatment of hypertension in African patients. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Eighty-one adult Nigerians with essential hypertension were randomly allocated to receive doxazosin, hydrochlorothiazide/amloride, or amlodipine. In each group, the patients were further classified as obese and non-obese, and total cholesterol as well as high density lipoprotein (HDL) cholesterol was determined before and after the 3-month treatment period. The total cholesterol level was significantly reduced in the non-obese patients, but did not show any significant change in the obese patients after doxazosin therapy, indicating the beneficial effects of doxazosin therapy in non-obese patients. The levels of total cholesterol increased and HDL cholesterol decreased in both the obese and the non-obese patients after hydrochlorothiazide/amloride therapy. Amlodipine treatment did not cause any significant change in the total and HDL cholesterol levels in both the obese and non-obese patients. These findings are worthy of consideration by clinicians and researchers when selecting the most appropriate drug for antihypertensive pharmacotherapy.
en-copyright= kn-copyright= en-aut-name=AhanekuJoseph Eberendu en-aut-sei=Ahaneku en-aut-mei=Joseph Eberendu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AgbedanaOlu Emmanuel en-aut-sei=Agbedana en-aut-mei=Olu Emmanuel kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaylorOladunni Grace en-aut-sei=Taylor en-aut-mei=Oladunni Grace kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Nnamdi Azikiwe University, Nigeria affil-num=2 en-affil= kn-affil=University of Ibadan, Nigeria affil-num=3 en-affil= kn-affil=University of Ibadan, Nigeria en-keyword=body mass index kn-keyword=body mass index en-keyword=cholesterol kn-keyword=cholesterol en-keyword=hypertension kn-keyword=hypertension en-keyword=African patients kn-keyword=African patients END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=237 end-page=240 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of pregnancy on plasma phenobarbital concentrations in rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We examined the pharmacokinetics of phenobarbital before and during pregnancy in rats. Animals were divided into four groups: (a) control, (b) pregnant, (c) phenobarbital-treated, and (d) phenobarbital-treated pregnant groups. The increase in body weight of nonpregnant or pregnant rats was not influenced by long-term phenobarbital treatment. Plasma phenobarbital concentrations during the period of long-term phenobarbital treatment with a fixed dosage by body weight were not significantly affected by pregnancy. Furthermore, pregnancy did not affect pharmacokinetic parameters of phenobarbital between 0.25 and 24h after administration. These results suggest that pregnancy does not influence on the pharmacokinetics of long-term phenobarbital treatment at a fixed dosage by body weight.
en-copyright= kn-copyright= en-aut-name=MoriyamaMasahiro en-aut-sei=Moriyama en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DomotoHaruyo en-aut-sei=Domoto en-aut-mei=Haruyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamashitaSyoichi en-aut-sei=Yamashita en-aut-mei=Syoichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FurunoKatsushi en-aut-sei=Furuno en-aut-mei=Katsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OishiRyozo en-aut-sei=Oishi en-aut-mei=Ryozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Kyushu University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=phenobarbital kn-keyword=phenobarbital en-keyword=pharmacokinetics kn-keyword=pharmacokinetics en-keyword=pregnancy kn-keyword=pregnancy en-keyword=plasma concentrations kn-keyword=plasma concentrations END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=241 end-page=245 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Combined therapy with interleukin 2 and indomethacin in mice inoculated with MH134 hepatoma. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The antitumor effects of indomethacin and interleukin 2 (IL-2) were studied in C3H/HeJ mice inoculated with MH134 hepatoma cells. Combined treatment with indomethacin and IL-2 augmented natural killer (NK) cells in mice with MH134-induced peritoneal carcinomatosis, and the survival of the treated mice was significantly longer than the non-treated mice. In animals with subcutaneous MH134 tumors, the combined therapy with indomethacin and IL-2 significantly suppressed tumor growth and induced complete regression of the tumor in three out of five mice. These results suggest that indomethacin and IL-2 therapy could be effective on human gastrointestinal cancer cells as well.
en-copyright= kn-copyright= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkamotoYasuhisa en-aut-sei=Okamoto en-aut-mei=Yasuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=GotohKiyotoshi en-aut-sei=Gotoh en-aut-mei=Kiyotoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HizutaAkio en-aut-sei=Hizuta en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YunokiShigeru en-aut-sei=Yunoki en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=indomethacin kn-keyword=indomethacin en-keyword=interleukin-2 kn-keyword=interleukin-2 en-keyword=mouse kn-keyword=mouse en-keyword=hepatoma kn-keyword=hepatoma END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=247 end-page=252 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Diuretic effect of cilazapril and dopamine system in the spontaneously hypertensive rat. en-subtitle= kn-subtitle= en-abstract= kn-abstract=<p>To assess the role of the kidney dopamine system on the diuretic state induced by angiotensin-converting enzyme (ACE) inhibitors, we examined the changes in urinary excretion and plasma level of dopamine, and kidney dopamine receptors in spontaneously hypertensive rats (SHR) treated with cilazapril, an ACE inhibitor. We administered cilazapril 10 mg/kg orally to 13-week-old SHR daily for 21 days (CILAZA group). Systolic blood pressure was significantly decreased in the CILAZA group on Day 6 compared with that in vehicle-treated SHR (control group). The urine volume was three- to fivefold higher in the CILAZA group, and total urinary dopamine secretion was also increased compared with the control group. There was no significant difference in affinity and number of kidney dopamine receptors between the CILAZA and the control groups. In conclusion, the diuretic effect caused by cilazapril is partly mediated by inhibition of the water reabsorption via the increase of dopamine production in the kidney.</p>
en-copyright= kn-copyright= en-aut-name=WatanabeHitoshi en-aut-sei=Watanabe en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OguraToshio en-aut-sei=Ogura en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HosoyaMasaharu en-aut-sei=Hosoya en-aut-mei=Masaharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishidaNorikazu en-aut-sei=Nishida en-aut-mei=Norikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=dopamine kn-keyword=dopamine en-keyword=ACE inhibitor kn-keyword=ACE inhibitor en-keyword=cilazapril kn-keyword=cilazapril en-keyword=SHR kn-keyword=SHR en-keyword=kidney kn-keyword=kidney END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=231 end-page=236 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Possible Roles of Nitric Oxide and Vasoactive Intestinal Polypeptide on Relaxation Induced by Isoprenaline in Isolated Muscle Strips of the Mouse Gastric Fundus en-subtitle= kn-subtitle= en-abstract= kn-abstract=The possible role of nitric oxide (NO) and vasoactive intestinal polypeptide on isoprenaline-induced relaxation of the mouse longitudinal gastric fundal strips precontracted with 5.4 x 10(-7) M carbachol was investigated. Isoprenaline (5 x 10(-7) M, 10(-6) M and 5 x 10(-6) M) produced a concentration-dependent relaxations. NG-nitro L-arginine (10(-4) M) partly inhibited isoprenaline-induced relaxation. The inhibitory action of NG-nitro L-arginine was reversed by 4 x 10(-4) M L-arginine but not by 4 x 10(-4) M D-arginine. NG-nitro L-arginine (10(-4) M) did not affect the relaxation caused by sodium nitroprusside (10(-6) M). Vasoactive intestinal polypeptide antibody 7913 (1:160 dilution) partly inhibited isoprenaline-induced relaxation. This inhibition was greater on the response to the higher isoprenaline concentration (5 x 10(-6) M) than to the lower concentration (10(-6) M). The combination of vasoactive intestinal polypeptide antibody and NG-nitro L-arginine significantly enhanced the inhibition on 10(-6) M isoprenaline action. These results suggest that nitric oxide and vasoactive intestinal polypeptide may partly contribute to the relaxation induced by isoprenaline in the mouse gastric fundus precontracted with carbachol.
en-copyright= kn-copyright= en-aut-name=OgulenerNuran en-aut-sei=Ogulener en-aut-mei=Nuran kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=LarabalEda en-aut-sei=Larabal en-aut-mei=Eda kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=BaysalFiruz en-aut-sei=Baysal en-aut-mei=Firuz kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=DikmenAtilla en-aut-sei=Dikmen en-aut-mei=Atilla kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Cukurova University affil-num=2 en-affil= kn-affil=Cukurova University affil-num=3 en-affil= kn-affil=Cukurova University affil-num=4 en-affil= kn-affil=Cukurova University en-keyword=isoprenaline kn-keyword=isoprenaline en-keyword= NG-nitro L-arginine(L-NOARG) kn-keyword= NG-nitro L-arginine(L-NOARG) en-keyword= L-arginine(L-ARG) kn-keyword= L-arginine(L-ARG) en-keyword= D-arginine(D-ARG) kn-keyword= D-arginine(D-ARG) en-keyword=vasoactive intestinal polypeptide (VIP) antibody 7913 kn-keyword=vasoactive intestinal polypeptide (VIP) antibody 7913 en-keyword=isolated mouse gastric fundus kn-keyword=isolated mouse gastric fundus END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=271 end-page=274 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of cigarette smoke on lipid peroxidation and liver function tests in rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effect of cigarette smoke on organ weights, lipid peroxidation and plasma biochemical parameters was investigated in male Wistar rats. Daily exposure (for 20 min twice a day) to cigarette smoke for 27 days caused a significant decrease in liver weight and a significant increase in lung weight. The smoke-exposure group showed increased lipid peroxidation in the liver, but not in the lung. In the smoke-exposure group, the GOT, gamma-GTP, total bilirubin and LDH values were significantly higher than those in the control group, while the plasma glucose value was significantly lower. These results suggest that cigarette smoking might induce liver injury by enhancing lipid peroxidation.
en-copyright= kn-copyright= en-aut-name=WatanabeKazuhide en-aut-sei=Watanabe en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EtoKoehi en-aut-sei=Eto en-aut-mei=Koehi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FurunoKatsushi en-aut-sei=Furuno en-aut-mei=Katsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoriTakaaki en-aut-sei=Mori en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Kawatetsu Mizushima Hospital affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=cigarette smoking kn-keyword=cigarette smoking en-keyword=lipid peroxidation kn-keyword=lipid peroxidation en-keyword=liver function kn-keyword=liver function en-keyword=rats kn-keyword=rats END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=253 end-page=260 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Multivisceral transplantation in pigs: a clinicopathological analysis of tissue rejection. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this study, we established the surgical procedure and postoperative care of multivisceral transplantation (MVTX) in pigs, and examined the functional changes and rejection pattern of transplanted organs in MVTX. Twenty-two MVTXs were performed without immunosuppression, and nine cases (41%) that survived for 5 days or more after MVTX were used for evaluation. Rejection in grafts including the liver, pancreas, and gastrointestinal tract were assessed histopathologically. On day 5 after transplantation, the duodenum and small bowel already showed signs of mild rejection. On the other hand, in the liver, pancreas and stomach, rejection occurred later and was still mild on day 16. Hepatic rejection in MVTX appeared to occur later than in simple liver transplantation (LTX). These results showed that the susceptibility to rejection of individual visceral organs varies.
en-copyright= kn-copyright= en-aut-name=MitsuokaShintaro en-aut-sei=Mitsuoka en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=multivisceral transplantation kn-keyword=multivisceral transplantation en-keyword=allograft kn-keyword=allograft en-keyword=rejection kn-keyword=rejection en-keyword=pigs kn-keyword=pigs END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=153 end-page=159 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Increased urinary excretion of non-albumin antigen detected with YO-2, a novel monoclonal antibody, in diabetic patients. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Monoclonal antibodies were raised against urine proteins from diabetic patients. An antibody, YO-2, stained three protein bands with apparent molecular weights of 66, 49, and 36 kDa. These bands were not reactive with an anti-human albumin antibody. The urine levels of YO-2-reactive antigen in the normal control were 0.97 +/- 0.37 U/g-Cr (units per gram of urine creatinine) (mean +/- SD). Those of the normo-, micro-, and macroalbuminuric diabetic patients, respectively, were 1.38 +/- 1.36, 2.87 +/- 2.07, and 3.92 +/- 3.33 U/g-Cr. They were significantly higher in the micro- and macroalbuminuric patients. The urine levels of YO-2-reactive antigen had no significant correlation with the urine albumin levels and hemoglobin A1c. We concluded that; a) monoclonal antibody YO-2 recognized a non-albumin urine antigen increasingly excreted in diabetic patients with nephropathy, b) recent glycemic control of diabetes would not significantly affect the urinary excretion rate of YO-2-reactive antigen, and c) the excretion rate and probably the mechanism of YO-2-reactive protein differed from those of albumin. The urine levels of YO-2-reactive antigen could be a clinical marker of diabetic nephropathy.
en-copyright= kn-copyright= en-aut-name=YoneiTaiji en-aut-sei=Yonei en-aut-mei=Taiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WataraiShinobu en-aut-sei=Watarai en-aut-mei=Shinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkadaYoshio en-aut-sei=Okada en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YasudaTatsuji en-aut-sei=Yasuda en-aut-mei=Tatsuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=diabetes kn-keyword=diabetes en-keyword=nephropathy kn-keyword=nephropathy en-keyword=monoclonal antibody kn-keyword=monoclonal antibody en-keyword=microalbuminuria kn-keyword=microalbuminuria END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=161 end-page=167 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Gene expression of liver-specific proteins in hepatocyte spheroids in primary culture. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Adult rat hepatocytes assemble to form multicellular spheroids under non-adherent environments such as immobilized chondroitin sulfate-proteoglycan in primary culture. Previously, we demonstrated that hepatocyte spheroids exhibited various differentiated structures as observed in the liver tissue. It was also shown that hepatocyte growth was highly suppressed and several differentiated functions, including albumin production and gluconeogenesis, were well preserved in spheroids. To investigate the differentiated functions of cultured hepatocytes in relation to cell morphology, we compared the expression of the albumin and transferrin genes in spheroids with those in monolayers by Northern blot analysis. Production of these proteins in the culture medium was simultaneously examined by ELISA. Gene expression and protein production of both albumin and transferrin were better preserved in spheroids. We also examined changes in the expression of liver-specific genes in response to IL-6. Reduced mRNA levels of both albumin and transferrin was only found in spheroids and no change was observed in monolayers. These results suggest that the regulation of tissue-specific gene expression is better preserved in spheroids, in which hepatocytes are in close contact with each other. en-copyright= kn-copyright= en-aut-name=TamuraTomoyuki en-aut-sei=Tamura en-aut-mei=Tomoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KoideNorio en-aut-sei=Koide en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HadaHajime en-aut-sei=Hada en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShirahaHidenori en-aut-sei=Shiraha en-aut-mei=Hidenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=hepatocyte kn-keyword=hepatocyte en-keyword=spheroid kn-keyword=spheroid en-keyword=primary culture kn-keyword=primary culture en-keyword=gene expression kn-keyword=gene expression en-keyword=IL-6 kn-keyword=IL-6 END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=123 end-page=127 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Human papillomavirus DNA in cell lines derived from malignancies. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The presence of high-risk types of human papillomavirus (HPV) 16, 18 and 33 in cell lines established from several malignancies including 5 of cervical cancer and 6 of head and neck cancer was studied. HPV DNA, either type 16 or 18, was detected by polymerase chain reaction, and by Southern blot hybridization in all of the cell lines derived from cervical cancers. The hybridization patterns of HPV DNA after endonuclease digestion differed among cell lines, suggesting that all of these cell lines were independent isolates. Accordingly, high-risk types of HPV DNA seem to be ubiquitous in cervical cancer. HPV DNA was not detected in the cell lines derived from head and neck cancers or from any other malignancies besides cervical cancer in this study.
en-copyright= kn-copyright= en-aut-name=ImuraMakoto en-aut-sei=Imura en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujiwaraTazuko en-aut-sei=Fujiwara en-aut-mei=Tazuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OguraHajime en-aut-sei=Ogura en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=human papillomavirus DNA kn-keyword=human papillomavirus DNA en-keyword=polymerase chain reaction kn-keyword=polymerase chain reaction en-keyword=Southern blot hybridization kn-keyword=Southern blot hybridization en-keyword=cell lines kn-keyword=cell lines en-keyword=cervical cancer kn-keyword=cervical cancer END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=175 end-page=178 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=31P nuclear magnetic resonance evaluation of rat liver preserved in UW solution. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A persistent problem in orthotopic liver transplantation is primary nonfunction (PNF) of the hepatic allograft. In an attempt to reduce the incidence of graft failure, the feasibility of pretransplant assessment of graft viability was investigated by 31P nuclear magnetic resonance (NMR) spectroscopy. The level of adenosine triphosphate (ATP) was measured as an indicator of liver function by 31P NMR spectroscopy after a 30 min normothermic reperfusion following cold-storage in University of Wisconsin (UW) solution. The mean +/- SD beta-ATP/Pi ratio after preservation for 0, 12, 24 or 48 h was 1.40 +/- 0.34, 0.85 +/- 0.27, 0.64 +/- 0.14 and 0.38 +/- 0.09, respectively. Significance was observed between 12h and 24h and between 12h and 48h of preservation. These results correlated well with the morphological changes in endothelial cells and sinusoidal lining cells examined by transmission electron microscopy. It is suggested strongly that microcirculatory disturbances due to endothelial cell injury impairs the recovery of ATP levels after reperfusion, and that ATP determination by 31P NMR spectroscopy, as a non-invasive modality, may help in the prediction of PNF after liver transplantation.
en-copyright= kn-copyright= en-aut-name=HamazakiKeisuke en-aut-sei=Hamazaki en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkamotoKo en-aut-sei=Okamoto en-aut-mei=Ko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=GochiAkira en-aut-sei=Gochi en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MatsubaraNagahide en-aut-sei=Matsubara en-aut-mei=Nagahide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MoriMasanobu en-aut-sei=Mori en-aut-mei=Masanobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=31P-NMR kn-keyword=31P-NMR en-keyword=liver preservation kn-keyword=liver preservation en-keyword=UW solution kn-keyword=UW solution END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=129 end-page=135 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Kinesiological Study of Push-up Motion Using a Three-Dimensional Floor Reaction on a Force Plate en-subtitle= kn-subtitle= en-abstract= kn-abstract=To find an effective way to handle wheelchairs, 3-dimensional floor reactions of the hand and angular deviation of the elbow and wrist joints during push-up motion were studied in 10 healthy men. The push-up was carried out using 3 hand positions (fist, finger and palm) and a push-up device. In all hand positions, anteroposterior force (Fx) and the mediolateral force (Fy) appeared after the vertical force (Fz). The end point of Fx and Fy was observed before that of Fz. Among the 4 different hand positions, Fx and Fy appeared first in the palm, followed by the finger and fist positions, and lastly in the push-up devices. The results indicate that the more unstable pushing-up the body is, the earlier and longer Fx and Fy are. Thus, Fx and Fy are considered to be good indicators of body balance during the push-up motion. The elbow joint showed a hyperextended position only when using the palm position in the maintenance phase. The wrist joint showed palmar flexion only when using the fist position.
en-copyright= kn-copyright= en-aut-name=IkawaHarutomo en-aut-sei=Ikawa en-aut-mei=Harutomo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TokuhiroAkihiro en-aut-sei=Tokuhiro en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=force plate kn-keyword=force plate en-keyword=push-up montion kn-keyword=push-up montion en-keyword=body balance kn-keyword=body balance en-keyword=three-dimensional floor reaction of the hand kn-keyword=three-dimensional floor reaction of the hand END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=137 end-page=144 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Virological and serological characterization of asymptomatic blood donors positive for anti-hepatitis C virus antibody. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To study the virological and serological characteristics of asymptomatic hepatitis C virus (HCV) carriers, 165 blood donors positive for antibody against HCV proteins by the second generation assay, were analyzed for their clinical backgrounds, serological reactivity against antigens derived from HCV by recombinant immunoblot assay, and the amount and genotype of HCV by the polymerase chain reaction. Compared with blood donors having abnormal levels of alanine aminotransferase (ALT), sera from the donors with normal levels of ALT reacted less frequently against NS4 antigens (anti-5-1-1: 34.4% vs. 54.5%, P = 0.0609; anti-c100-3: 34.4% vs. 56.1%, P < 0.05). Also the positivity for antibodies against these antigens were more frequent in sera from donors with genotype 1b HCV-RNA than other genotypes (anti-5-1-1: 61.0% vs. 23.5%, P < 0.01; anti-c 100-3: 61.0% vs. 26.5%, P < 0.01). The prevalence of each genotype in blood donors with normal ALT levels was different from that in patients with advanced liver disease (P < 0.05), genotype 1b being less and genotype 2a being more frequent. The number of HCV-RNA copies/0.5 ml in donors with normal ALT was 10(7.9 +/- 1.0) (n = 27) and that in patients with chronic liver disease was 10(7.4 +/- 0.8) (n = 116), the difference being statistically significant (P < 0.05). In conclusion, the results of this study suggest that asymptomatic blood donors carrying HCV have the serological and virological characteristics different from the patients with advanced liver disease.
en-copyright= kn-copyright= en-aut-name=TsujiHideyuki en-aut-sei=Tsuji en-aut-mei=Hideyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShimomuraHiroyuki en-aut-sei=Shimomura en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatoMasaki en-aut-sei=Wato en-aut-mei=Masaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KondoJunichi en-aut-sei=Kondo en-aut-mei=Junichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=hepatitis C virus kn-keyword=hepatitis C virus en-keyword=blood donor kn-keyword=blood donor en-keyword=asymptomatic carrier kn-keyword=asymptomatic carrier END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=117 end-page=121 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Serum bleomycin-detectable iron in patients with thalassemia major with normal range of serum iron. en-subtitle= kn-subtitle= en-abstract= kn-abstract="Free" iron, a potentially radical-generating low mass iron, and not found in normal human blood, was increased in the serum of blood-transfused thalassemia major patients seen in the Yangon General Hospital, Yangon, Myanmar (Burma). The low mass iron was detected by the bleomycin assay. Fifty-one blood samples were analyzed (from 28 males and 23 females). High "free" iron was detected in 47 sera samples from thalassemia patients. Serum ferritin, which reflects the body store iron, was higher than the normal range (10-200 ng/ml) in 49 patients. On the other hand, serum iron of 39 sera samples fell within the normal range (50-150 micrograms/dl). Four were less than 50 micrograms/dl and eight were more than 150 micrograms/dl. Almost all the patients' sera of normal or higher serum iron level contained "free" iron. Thus, almost all the sera from thalassemic patients from Myanmar contain bleomycin-detectable iron, even when serum iron is within the normal range. In developing countries where undernutrition is prevalent (serum albumin in these patients was 3.6 +/- 0.4 g/dl, P < 0.0001 vs. control value of 4.0 - 4.8 g/dl), normal serum iron does not preclude the presence of free iron in the serum.
en-copyright= kn-copyright= en-aut-name=HanKhin Ei en-aut-sei=Han en-aut-mei=Khin Ei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkadaShigeru en-aut-sei=Okada en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=thalassemia kn-keyword=thalassemia en-keyword=free iron kn-keyword=free iron en-keyword=hemochromatosis kn-keyword=hemochromatosis en-keyword=iron overload kn-keyword=iron overload en-keyword=serum iron kn-keyword=serum iron en-keyword=bleomycin-detectable iron kn-keyword=bleomycin-detectable iron en-keyword=free radical kn-keyword=free radical END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=169 end-page=173 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mechanical left ventricular support in patients with Marfan's syndrome: a report of two cases. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Left ventricular assist device (LVAD) was utilized for the treatment of postcardiotomy heart failure in two patients with Marfan's syndrome. Patient 1 (a 22-year-old) with annuloaortic ectasia (AAE) and DeBakey type II dissection had been supported by LVAD for 87h after composite graft replacement of the ascending aorta and aortic valve. Patient 2 (a 52-year-old) with AAE and DeBakey type I dissection had been supported by LVAD for 91 h after aortic valve replacement. During the assist, both patients complicated bleeding from the fragile left atria near the sites of cannulation. Patient 1 died of multiple organ failure on the 62nd postoperative day, but patient 2 returned to work after surgery.
en-copyright= kn-copyright= en-aut-name=IshinoKozo en-aut-sei=Ishino en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurakamiTaiji en-aut-sei=Murakami en-aut-mei=Taiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IrieHiroyuki en-aut-sei=Irie en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KawakamiShunji en-aut-sei=Kawakami en-aut-mei=Shunji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SenooYoshimasa en-aut-sei=Senoo en-aut-mei=Yoshimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TeramotoShigeru en-aut-sei=Teramoto en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=ventricular assist device kn-keyword=ventricular assist device en-keyword=Marfan's syndrome kn-keyword=Marfan's syndrome en-keyword=aortic dissection kn-keyword=aortic dissection END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=145 end-page=151 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Expression of ICAM-I on M cells covering isolated lymphoid follicles of the human colon. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the immunological function of 'M' (microfold or membranous) cells in the large intestine, we examined the expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-class II antigens immunohistochemically in M cells and follicle-associated epithelia (FAE) covering isolated lymphoid follicles of the human colon in comparison with their expression in Peyer's patches of the small intestine. In Peyer's patches of the small intestine, ICAM-1 was not expressed on the epithelial cells covering the lymphoid follicles, but their cell surfaces were stained positively for HLA-DR. In contrast, colonic M cells expressed ICAM-1 on their cell surfaces but were negative for HLA class II antigens. By immunoelectron microscopy, ICAM-1 was seen to be distributed on the surface of microfolds, on the membranes of apical vesicles and on part of the basolateral plasma membranes of M cells, but was not expressed on adjacent FAE. These findings imply that the M cells in the colon and in Peyer's patches have different immunological roles. In addition, identification of ICAM-1 expression on the colonic M cells should help elucidate the pathogenesis of some inflammatory colonic diseases which appear to start in the lymphoid follicles of the colonic mucosa.
en-copyright= kn-copyright= en-aut-name=UekiToru en-aut-sei=Ueki en-aut-mei=Toru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MizunoMotowo en-aut-sei=Mizuno en-aut-mei=Motowo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UesuTokurou en-aut-sei=Uesu en-aut-mei=Tokurou kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KisoTakahiko en-aut-sei=Kiso en-aut-mei=Takahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univresity affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=ICAM-I kn-keyword=ICAM-I en-keyword=M cell kn-keyword=M cell en-keyword=follicle-associated epithelial cells kn-keyword=follicle-associated epithelial cells en-keyword=HLA antigen kn-keyword=HLA antigen en-keyword=immunoelectron microscopy kn-keyword=immunoelectron microscopy END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=35 end-page=42 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A method for determination of total glutathione and total cysteine as S-carboxymethyl derivatives by using an amino acid analyzer, and its application to samples from rat liver, kidney and blood after intraperitoneal administration of 2-(4-carboxy-D-gluco-tetrahydroxybutyl)thiazolidine-4-carboxylic acid. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of intraperitoneal administration of 2-(4-carboxy-D-gluco-tetrahydroxybutyl)thiazolidine-4-carboxylic acid (CGUA), a cysteine derivative conjugated with glucuronic acid, on total glutathione and total cysteine contents in rat tissues were investigated. Total glutathione (GSH and GSSG) and total cysteine (cysteine and cystine) were determined by a new method consisting of preparation of S-carboxymethylglutathione (CMSG) and S-carboxymethylcysteine (CMC), respectively, and subsequent analyses with an amino acid analyzer. CGUA was determined by a coloration method employing an acidic ninhydrin reagent. Total cysteine contents in liver, kidney and plasma rapidly increased to 2.3, 2.7 and 6.5 times the levels of the controls, respectively, after CGUA administration at a dose of 5 mmol/kg of body weight. Total glutathione content did not change significantly in the liver or blood except for the kidney with a significant increase during the first 1-h period after administration. CGUA content increased markedly in these tissues, especially in the kidney, and 30% of administered CGUA was excreted in urine within 2h. These results indicate that CGUA is converted into cysteine in vivo, suggesting the usefulness of this compound for protection of the kidney and the liver.
en-copyright= kn-copyright= en-aut-name=ZhaoYuan-Qing en-aut-sei=Zhao en-aut-mei=Yuan-Qing kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KinutaMasahiro en-aut-sei=Kinuta en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AbeTadashi en-aut-sei=Abe en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YaoWen-Bin en-aut-sei=Yao en-aut-mei=Wen-Bin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=UbukaToshihiko en-aut-sei=Ubuka en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=cysteine kn-keyword=cysteine en-keyword=glutathione kn-keyword=glutathione en-keyword=S-carboxymethylglutathione kn-keyword=S-carboxymethylglutathione en-keyword=S-carboxymethylcysteine kn-keyword=S-carboxymethylcysteine en-keyword=cysteineglucuronic acied kn-keyword=cysteineglucuronic acied END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=43 end-page=51 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Finite element analysis of pathogenesis of osteoarthritis in the first carpometacarpal joint. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Stress distribution in the first carpometacarpal joint was analyzed in 49 cadaveric hands using the finite element method to clarify the pathogenesis of osteoarthritis in the joint. The results of the finite element method analysis were compared with those of the contact pressure distribution in the first carpometacarpal joint of cadaveric specimens using pressure-sensitive film, and with the simple roentgenographical and microradiographical manifestations of spur formation, and with histological findings of osteoarthritis to verify the accuracy of the models of computer simulation models. The comparison of these results showed that osteoarthritic changes of the first carpometacarpal joint were found in areas where stress was concentrated during movement of the joint. The saddle shape of this joint is essentially well-designed for the dispersion of normal stress, however minimal displacement due to instability could easily induce osteoarthritis. Furthermore the shallow trapezial configuration may contribute to the high incidence of osteoarthritis changes. The finite element method helped clarify the relationship between stress patterns and osteoarthritis response.
en-copyright= kn-copyright= en-aut-name=ShiQilin en-aut-sei=Shi en-aut-mei=Qilin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HashizumeHiroyuki en-aut-sei=Hashizume en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyakeToshiyuki en-aut-sei=Miyake en-aut-mei=Toshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NagayamaNoriyuki en-aut-sei=Nagayama en-aut-mei=Noriyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Industrial Technology Center of Okayama Prefecture en-keyword=carpometacarpal joint kn-keyword=carpometacarpal joint en-keyword=onset mechanism of osteoarthritis kn-keyword=onset mechanism of osteoarthritis en-keyword=stress distribution analysis kn-keyword=stress distribution analysis en-keyword=twodimensinoal finite element method kn-keyword=twodimensinoal finite element method en-keyword=pressure-sensitive film kn-keyword=pressure-sensitive film END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=13 end-page=17 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Neurotransmitter interactions in the striatum and hypothalamus of mice after single and repeated ethanol treatment en-subtitle= kn-subtitle= en-abstract= kn-abstract=In single treatment study, ethanol was administered intraperitoneally to ICR mice (about 34 g) in the amounts of 1.0, 2.0, 3.0 or 4.0 g/kg body weight. The 3,4-dihydroxyphenylacetic acid (DOPAC) + homovanillic acid (HVA) concentration in the striatum was elevated with 3.0 and 4.0 g/kg of ethanol. In the hypothalamus, the DOPAC, HVA and 5-hydroxyindoleacetic acid concentrations were increased after injection of 3.0 and 4.0 g/kg of ethanol. Furthermore, the acetylcholine (ACh) and gamma-aminobutyric acid (GABA) concentrations were also increased following the injection of 1.0, 2.0, 3.0 and 4.0 g/kg. To study the effects of repeated administration, mice were injected intraperitoneally with 1.0 or 2.0 g/kg of ethanol once daily for 7 days. The DOPAC + HVA level in the striatum was elevated after injection of 1.0 and 2.0 g/kg of ethanol. The GABA and ACh concentrations in the hypothalamus were decreased after repeated injections of ethanol. These results suggest that ethanol significantly alters the utilization of dopamine, ACh and GABA in the hypothalamus. This may partially explain why ethanol has such profound effects on emotional behavior and mood.
en-copyright= kn-copyright= en-aut-name=KaneyukiTakao en-aut-sei=Kaneyuki en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MorimasaTadaomi en-aut-sei=Morimasa en-aut-mei=Tadaomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShohmoriToshikiyo en-aut-sei=Shohmori en-aut-mei=Toshikiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama Prefectural University affil-num=2 en-affil= kn-affil=Okayama Prefectural University affil-num=3 en-affil= kn-affil=Okayama Prefectural University en-keyword=ethanol kn-keyword=ethanol en-keyword=dopamine kn-keyword=dopamine en-keyword=serotonin kn-keyword=serotonin en-keyword= ?-aminobutyric acid kn-keyword= ?-aminobutyric acid en-keyword=acetylcholine kn-keyword=acetylcholine en-keyword=striatum kn-keyword=striatum en-keyword=hypothalamus kn-keyword=hypothalamus END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=19 end-page=24 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Immunohistochemical studies of PIVKA-II in hepatocellular carcinoma by indirect immunofluorescence en-subtitle= kn-subtitle= en-abstract= kn-abstract=Tissue PIVKA-II was examined in 32 hepatocellular carcinomas and 2 metastatic liver tumors using indirect immunofluorescence, and the results were compared with the size, histological grading and serum PIVKA-II level. The specificity of this method was confirmed by the disappearance of reactivity in PLC/PRF/5 cells after the addition of vitamin K to the culture medium. Positive PIVKA-II staining was observed as a clustered or a single cell pattern only in the HCC nodules, but not in the surrounding cirrhotic tissue. PIVKA-II staining was observed in all HCC groups regardless of histological grade. There was no relationship between PIVKA-II staining and the size of HCC. PIVKA-II was detected immunohistochemically even in small HCC of patients whose plasma PIVKA-II levels were below the detection limit. These results suggest that PIVKA-II production is a specific phenotype of HCC regardless of its histological grading and demonstrate that this immunofluorescent PIVKA-II staining is more sensitive and useful than plasma PIVKA-II assay for the diagnosis of HCC.
en-copyright= kn-copyright= en-aut-name=KuwaharaNaoaki en-aut-sei=Kuwahara en-aut-mei=Naoaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HigashiToshihiro en-aut-sei=Higashi en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NousoKazuhiro en-aut-sei=Nouso en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ItoToshio en-aut-sei=Ito en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma en-keyword=PIVKA-??immunofluorescent staining kn-keyword=PIVKA-??immunofluorescent staining en-keyword=tumor marker kn-keyword=tumor marker END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=25 end-page=28 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Karyotypic analysis in the process of immortalization of human cells treated with 4-nitroquinoline 1-oxide en-subtitle= kn-subtitle= en-abstract= kn-abstract=The establishment of a model system of neoplastic transformation of normal human cells has been attempted with a chemical carcinogen, 4-nitroquinoline 1-oxide (4NQO). In the course of these experiments, it was noticed that immortalization of human cells is a multi-step process involving several mutational genetic events. Thus, chromosomal changes which occurred during the process of immortalization of human fibroblasts were examined. To accomplish immortalization, fibroblasts obtained from an embryo were repeatedly treated with 10-6M4NQO from primary culture to passage 51 (59 treatments in total). Before immortalization, some chromosomes (especially, chromosomes 2, 6, 8, 10, 11, 12, 15, 19, and 20), were lost at a relatively high frequency. After immortalization, the chromosomes distributed so broadly in the triploid to hypotetraploid region without a distinct modal number or without marker chromosomes that it was difficult to identify the specific chromosomes related to the immortalization of human cells. No specific structural chromosomal changes were detected. Although the significance of such chromosome changes in relation to immortalization is not clear, the loss of some specific chromosomes suggests that genes which are involved in cellular aging and which suppress immortalization may have been lost in the immortalization process.
en-copyright= kn-copyright= en-aut-name=JahanIsrat en-aut-sei=Jahan en-aut-mei=Israt kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=BaiLiyan en-aut-sei=Bai en-aut-mei=Liyan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IijimaMikio en-aut-sei=Iijima en-aut-mei=Mikio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KondoTadashi en-aut-sei=Kondo en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NambaMasayoshi en-aut-sei=Namba en-aut-mei=Masayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=human cells kn-keyword=human cells en-keyword=chromosomes kn-keyword=chromosomes en-keyword=aging kn-keyword=aging en-keyword=immortalization kn-keyword=immortalization en-keyword=4NQO kn-keyword=4NQO END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=1 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Molecular and chemical neuropharmacology of dopamine receptor subtypes en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the fields of psychiatry and neurology, the dopaminergic system is one of the most important neurotransmitter systems in the brain. Whereas pharmacological and biochemical studies had initially indicated two subclasses of dopamine receptors (DA-R), recent progress in molecular biology techniques has led to the identification of five distinct genes of DA-Rs (D1-R-D5-R) and splice variants. The gene products are classified into the D1-R family (D1-R and D5-R) and D2-R family (D2-R, D3-R and D4-R) based on their structure and pharmacological features. This review summarizes the structure, localization, function and pharmacology of DA-R subtypes on the basis of knowledge obtained during the past few years. The genes encoding the D1-R family have no intron and the D2-R family genes have introns. The distributions of mRNAs encoding these five DA-R subtypes in the brain were different from their respective receptors. The localization of DA-R subtypes to particular brain regions and specific pharmacological profiles of DA-R subtypes allow new insights to be made into the mechanism of action of DA in the control of psychiatric and motor functions. The availability of detailed information about DA-R subtypes will not only clarify their roles in the brain, but will probably also lead to the development of new therapeutic drugs with more specific actions.
en-copyright= kn-copyright= en-aut-name=OgawaNorio en-aut-sei=Ogawa en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=dopamine receptor subtype kn-keyword=dopamine receptor subtype en-keyword=gene kn-keyword=gene en-keyword=molecular structure kn-keyword=molecular structure en-keyword=localization kn-keyword=localization en-keyword=pharmacology kn-keyword=pharmacology END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=53 end-page=59 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Enhanced pressor response in spontaneously hypertensive rats induced by stimulation of vasopressin-V1 receptors. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To elucidate the effect of the arginine vasopressin (AVP) system in vivo, especially V1 and V2 activity, on blood pressure, we measured the acute changes in blood pressure and heart rate after AVP, OPC-21,268 (a V1 receptor antagonist), and OPC-31,260 (a V2 receptor antagonist) were injected intravenously in anesthetized spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats at the age of 15 weeks. Compared with the control period, single injection of AVP 5 ng/kg significantly increased systolic blood pressure in WKY rats without a concomitant increase in heart rate, but there was no significant increase in blood pressure in SHR. In contrast, single injection of either OPC-21,268 3 mg/kg or OPC-31,260 3 mg/kg did not affect blood pressure or heart rate in either SHR or WKY rats. Injection of AVP after the administration of OPC-31,260 induced a greater increase in blood pressure in SHR than in WKY rats, whereas injection of AVP after the administration of OPC-21,268 did not induce any clear increase in blood pressure in SHR or WKY rats. These results suggest that SHR have enhanced pressor activity mediated by V1 receptors and that this increase may be due to an increase in their number. In conclusion, enhancement of V1 activity may contribute to the development of high blood pressure in SHR.
en-copyright= kn-copyright= en-aut-name=YamauchiTakayoshi en-aut-sei=Yamauchi en-aut-mei=Takayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OguraToshio en-aut-sei=Ogura en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OishiTetsuya en-aut-sei=Oishi en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HaradaKazushi en-aut-sei=Harada en-aut-mei=Kazushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HashimotoMasami en-aut-sei=Hashimoto en-aut-mei=Masami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MimuraYukari en-aut-sei=Mimura en-aut-mei=Yukari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AsanoNaoko en-aut-sei=Asano en-aut-mei=Naoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KageyamaJingo en-aut-sei=Kageyama en-aut-mei=Jingo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univresity affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University en-keyword=vasopressin kn-keyword=vasopressin en-keyword=V1 and V2 receptor antagonist kn-keyword=V1 and V2 receptor antagonist en-keyword=hypertension kn-keyword=hypertension en-keyword=pressor response kn-keyword=pressor response en-keyword=OPC-31260 kn-keyword=OPC-31260 END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=29 end-page=33 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Major Basic Protein, Eosinophil Cationic Protein, and Arylsulfatase in Nasal Secretions of Patients with Japanese Cedar Pollinosis en-subtitle= kn-subtitle= en-abstract= kn-abstract=In 15 patients with Japanese cedar pollinosis and 10 healthy control subjects, levels of major basic protein (MBP), eosinophil cationic protein (ECP), and arylsulfatase B (As) in the nasal secretions were examined before and after challenge with Japanese cedar pollen extract. The MBP and ECP levels in the patients were significantly higher 30 min after challenge than those before challenge (P < 0.005). MBP and ECP levels after challenge were significantly higher in the nasal secretions of patients than in the controls (MBP: P < 0.01, ECP: P < 0.05). The level of As after challenge was significantly higher in the nasal secretions of patients than in the controls. These results suggest that eosinophils activate or modify the immediate, nasal allergic reaction and have a role in regulating immunological responses.
en-copyright= kn-copyright= en-aut-name=NishiokaKeiko en-aut-sei=Nishioka en-aut-mei=Keiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OgawaTeruhiro en-aut-sei=Ogawa en-aut-mei=Teruhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SaitoChisato en-aut-sei=Saito en-aut-mei=Chisato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishiokaSatoko en-aut-sei=Nishioka en-aut-mei=Satoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakagawaFumio en-aut-sei=Nakagawa en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OhomichiTakuya en-aut-sei=Ohomichi en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MasudaYu en-aut-sei=Masuda en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Hiroshima City Hospital affil-num=2 en-affil= kn-affil=Hiroshima City Hospital affil-num=3 en-affil= kn-affil=Okayama City Hospital affil-num=4 en-affil= kn-affil=Okayama University Medical School affil-num=5 en-affil= kn-affil=Okayama University Medical School affil-num=6 en-affil= kn-affil=Okayama University Medical School affil-num=7 en-affil= kn-affil=Okayama University Medical School en-keyword=major basic protein kn-keyword=major basic protein en-keyword=eosinophil cationic protein kn-keyword=eosinophil cationic protein en-keyword=arylsulphatase B kn-keyword=arylsulphatase B en-keyword=pollinosis kn-keyword=pollinosis en-keyword=allergic rhinitis kn-keyword=allergic rhinitis END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=4 article-no= start-page=217 end-page=222 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synergistic Antiproliferative Effects of the Combination of Natural Human Tumor Necrosis Factor-α and Natural Murine Interferon-α/β against Colon-26 Adenocarcinoma Hepatic Metastases in a Murine Model en-subtitle= kn-subtitle= en-abstract= kn-abstract=To prevent the development of hepatic metastases after surgery for colorectal cancer, it is important to inhibit the growth of any micrometastases which occur during the operation. We used a hepatic metastasis model in mice to investigate the effects of combination therapy with natural human tumor necrosis factor-alpha (nHuTNF-alpha) and natural murine interferon-alpha/beta (nMuIFN-alpha/beta). Decreased formation of hepatic metastases by murine colon-26 carcinoma was recognized following a single injection of nHuTNF-alpha, nMuIFN-alpha/beta, or both. These inhibitory effects were synergistic. NK activity was also measured, because notaral lerller cells not only have an anti-tumor effect but are also a representative of the host immune system. Both nHuTNF-alpha and nMuIFN-alpha/beta were able to activate NK cells, and the combination of the cytokines more significantly augmented NK activity. The in vivo elevation of NK activity induced by nHuTNF-alpha, nMuIFN-alpha/beta, or their combination may be one of the mechanisms of their antiproliferative effect on experimental hepatic metastases of murine colon-26 carcinoma.
en-copyright= kn-copyright= en-aut-name=SanadaEiji en-aut-sei=Sanada en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FuchimotoSadanori en-aut-sei=Fuchimoto en-aut-mei=Sadanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univresity en-keyword=nHuTNF-? kn-keyword=nHuTNF-? en-keyword= nMuIFN-?/? kn-keyword= nMuIFN-?/? en-keyword=antiproliferative effect kn-keyword=antiproliferative effect en-keyword=hepatic metastasis kn-keyword=hepatic metastasis en-keyword=NK acitivity kn-keyword=NK acitivity END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=4 article-no= start-page=223 end-page=231 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A new open reduction treatment for congenital hip dislocation: long-term follow-up of the extensive anterolateral approach. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Congenital hip dislocation, which is conservatively unmanageable, has usually been treated using open reduction. However, a long-term follow-up study of the results suggests that this procedure is unsatisfactory. Since 1973, Tanabe has used a new open reduction procedure that circumferentially dissects the joint capsule and produces sufficient concentric reduction of the femoral head in the acetabulum immediately after the surgery. Fifty-six children (65 hips) from the age of 1 to 3 years were treated by this procedure, and fifty-one of them were clinically and roentgenographically followed up from 6.3 to 12.4 years after the surgery. At the final follow-up session, all children had grown to be over 9 years of age, and no patient had clinically significant symptoms. According to Severin's classification, 33 hips were rated in Group I, and 14 hips in Group II. Another 10 hips were in Group III, and one hip was in Group IV. The incidence of avascular necrosis was 5.2 per cent. These data suggest that our procedure is more useful than the previous ones. en-copyright= kn-copyright= en-aut-name=AkazawaHirofumi en-aut-sei=Akazawa en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanabeGozo en-aut-sei=Tanabe en-aut-mei=Gozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyakeYoshimasa en-aut-sei=Miyake en-aut-mei=Yoshimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Orthopaedic Surgery, Okayama University Medical School affil-num=2 en-affil= kn-affil=Department of Orthopaedic Surgery, Okayama University Medical School affil-num=3 en-affil= kn-affil=Department of Orthopaedic Surgery, Okayama University Medical School en-keyword=congenital hip dislocation kn-keyword=congenital hip dislocation en-keyword=new open reduction kn-keyword=new open reduction en-keyword=follow-up study kn-keyword=follow-up study en-keyword=anterolateral approach kn-keyword=anterolateral approach END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=4 article-no= start-page=171 end-page=186 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Carbohydrate metabolism of rats with biliary obstruction. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Carbohydrate metabolism of rats with obstructive jaundice caused by bile duct ligation was studied by intravenous glucose tolerance test (IVGTT) and by liver perfusion. The altered levels of carbohydrate-metabolizing enzyme were examined in relation to the glucose metabolism of the cholestatic rats. In the IVGTT, the rate of fractional glucose removal was increased with increases in plasma insulin and glucagon and with a decrease in non-esterified fatty acid. In liver perfusion, neither the glucose uptake nor insulin extraction by the whole liver of icteric rats was different from the control. The increased rate of glucose removal in IVGTT may be due to enhanced glucose utilization by peripheral tissues resulting from hypersecretion of insulin. In liver perfusate supplemented with glucose, a decrease in the glucose uptake per unit liver weight was observed in relation to the lowered glucokinase activity. Formation of glycogen from glucose and of glucose from lactate was also impaired, indicating inhibition of the gluconeogenic system or relative hyperfunction of the glycolytic system, which may further contribute to the reduction in glycogen content. These metabolic disorders correlated well with the changes in activities of key carbohydrate-metabolizing enzymes, which showed a characteristic pattern consistent with the loss of differentiated hepatic functions. Uptake of glucose and its conversion to glycogen were reduced in the cholestatic liver in close association with altered activities of some of related enzymes. However, due to increased utilization by the peripheral tissues, the total amount of glucose utilized in the whole rat was not reduced.
en-copyright= kn-copyright= en-aut-name=KimHitoshi en-aut-sei=Kim en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MimuraHisashi en-aut-sei=Mimura en-aut-mei=Hisashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=carbohydrate metabolism kn-keyword=carbohydrate metabolism en-keyword=obstructive jaundice kn-keyword=obstructive jaundice en-keyword=liver perfusion kn-keyword=liver perfusion en-keyword=intravenous glucose tolerance test kn-keyword=intravenous glucose tolerance test en-keyword=glycogen kn-keyword=glycogen END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=4 article-no= start-page=187 end-page=201 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cell kinetic analysis of brain tumors using the monoclonal antibody Ki-67: in vitro and in situ study. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ki-67 is a commercially available mouse monoclonal antibody (MoAb), which reacts with a nucleolar antigen (the Ki-67 antigen) expressed in proliferating eukaryotic cells. The author examined the precise localization of the Ki-67 antigen in C-6 cells using immunohistochemical and immunoelectron microscopic methods and estimated the proliferative activity of human brain tumors in situ. Positive nucleoplasmic reactions (early G1 phase) and nucleolar staining (late G1 phase) were observed. The cells showed very weak positive reactions in only one or two nucleoli (S phase) and multiple spicule reactions in the nucleoplasm (G2 phase). During the mitotic phase, the Ki-67 antigen was stained on the surfaces of all chromosomes and finely dispersed in the cytoplasm. By immunoelectron microscopic study, positive reactions were observed on the granular and dense fibrillar components. Therefore, the Ki-67 antigen seems to participate in the processing and assembly of preribosomal particles. In human brain tumors, the Ki-67 score (positive cells/total neoplastic cells), ranging 0 to 36.7%, correlated well with the histopathological grade of malignancy of the tumor. These findings suggest that immunohistochemical staining with the MoAb Ki-67 can be used as a convenient procedure for the simple evaluation of the proliferative activity of brain tumors.
en-copyright= kn-copyright= en-aut-name=ShiraishiTetsuya en-aut-sei=Shiraishi en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=monoclonal antibody Ki-67 kn-keyword=monoclonal antibody Ki-67 en-keyword=immunohistochemistry kn-keyword=immunohistochemistry en-keyword=cell proliferation kn-keyword=cell proliferation en-keyword=brain tumors kn-keyword=brain tumors en-keyword=nucleolar organizer regions kn-keyword=nucleolar organizer regions END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=4 article-no= start-page=203 end-page=208 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fetal calf serum increased the zona pellucida penetrability of rat oocytes matured in vitro. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We examined the effect of fetal calf serum (FCS) on meiotic division, subsequent fertilization, and first cleavage to the 2-cell stage of rat oocytes during in vitro maturation. FCS had no effect on the nuclear progression from dictiate to metaphase of the second maturation in vitro and, FCS had no effect on the first cleavage to the 2-cell stage of fertilized oocytes. However, FCS efficiently increased penetration rate of oocytes and shortened the time required for dissolution of the zona pellucida by alpha-chymotrypsin. These results showed that FCS did not affect cytoplasmic maturation necessary for oocytes to develop to the 2-cell stages. We found that FCS only affects the zona pellucida and does not affect the nucleus or cytoplasm of rat oocytes. FCS may prevent hardening of the zona pellucida.
en-copyright= kn-copyright= en-aut-name=FujiiYoshitaka en-aut-sei=Fujii en-aut-mei=Yoshitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshiokaTamotsu en-aut-sei=Yoshioka en-aut-mei=Tamotsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Center for Adult Diseases en-keyword=in vitro fertilization kn-keyword=in vitro fertilization en-keyword=in vitro maturation kn-keyword=in vitro maturation en-keyword=fetal calf serum kn-keyword=fetal calf serum en-keyword=rat kn-keyword=rat en-keyword=zona pellucida kn-keyword=zona pellucida END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=4 article-no= start-page=161 end-page=169 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Neural regulation of the interrelation between haustral and taenial motility in the rabbit proximal colon. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Neural regulation of the motility between the haustra and taenia coli was studied in the isolated rabbit proximal colon. Four types of haustral and taenial preparations were used: the haustral strip without the taenia coli (type 1), the haustral strip including the taenia coli (type 2), the L-shaped (taenia-haustra) preparations for recording the haustral (circular) response to taenial stimulation (type 3) and the L-shaped (haustra-taenia) preparation for recording the taenial (longitudinal) response to haustral stimulation (type 4). Field electrical stimulation induced a contractile response in the haustra and taenia coli. Hexamethonium reduced the contraction in type 2, 3 and 4 preparations. The desensitization to serotonin reduced the response in type 2 and 3 preparations. After atropinization, the response in types 1 and 4 was reversed to relaxation, and the response in types 2 and 3 was reversed to relaxation followed by contraction which was reduced or abolished by indomethacin. The responses remaining after atropinization in all types of preparations were not affected by other blocking agents tested or desensitization to neuropeptides. Tetrodotoxin abolished all relaxation and contractile responses in all types of preparations. These results suggest that the indirect contractile response to field stimulation is induced mainly via cholinergic and serotonergic neurons, and that the relaxation is mainly mediated by nonadrenergic noncholinergic neurons. The late haustral contractions after atropine may be caused by endogenous prostaglandin.
en-copyright= kn-copyright= en-aut-name=WatanebaYoshinori en-aut-sei=Wataneba en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamasatoTeruhiro en-aut-sei=Yamasato en-aut-mei=Teruhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakayamaSosogu en-aut-sei=Nakayama en-aut-mei=Sosogu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=proximal colon kn-keyword=proximal colon en-keyword=cholinergic neuron kn-keyword=cholinergic neuron en-keyword=serotonergic neuron kn-keyword=serotonergic neuron en-keyword=nonadrenergic noncholinergic neuron kn-keyword=nonadrenergic noncholinergic neuron en-keyword=prostaglandin kn-keyword=prostaglandin END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=4 article-no= start-page=209 end-page=215 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Interaction of Sevoflurane, Isoflurane, Enflurane and Halothane with Non-Depolarizing Muscle Relaxants and their Prejunctional Effects at the Neuromuscular Junction en-subtitle= kn-subtitle= en-abstract= kn-abstract=The interaction of four inhalational anesthetics (sevoflurane, isoflurane, enflurane and halothane) with pancuronium and vecuronium and also their prejunctional actions at the neuromuscular junction were quantitatively studied using rat phrenic nerve-hemidiaphragm preparations. To investigate the prejunctional effects of inhalational anesthetics, a train-of-four ratio (T4/T1) and the tetanus ratio (the ratio of the final response to the initial response during tetanus) were evaluated. All four inhalational anesthetics markedly potentiated the neuromuscular blockade of twitch response caused by either pancuronium or vecuronium with halothane and enflurane being the most potent both on a % concentration basis and on a MAC (minimum alveolar concentration) basis. Although none of the four inhalational anesthetics had any effects on the T4/T1 ratio, they produced variable effects on the tetanus ratio. Sevoflurane had little effect on the tetanus ratio, whereas 1 and 2% isoflurane and 1, 2 and 3% enflurane increased the tetanus ratio and 5% halothane and 5% enflurane significantly reduced the tetanus ratio. Halothane and enflurane had the most potent depressant action of the four inhalational anesthetics both on the % concentration basis and on the MAC basis. These results indicate that the main site of action of inhalational anesthetics is a postjunctional site at the neuromuscular junction and that they do not seem to act on prejunctional sites at the concentrations used in clinical situations. en-copyright= kn-copyright= en-aut-name=KobayashiOsamu en-aut-sei=Kobayashi en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhtaYoshio en-aut-sei=Ohta en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KosakaFutami en-aut-sei=Kosaka en-aut-mei=Futami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Anesthesiology and Resuscitology, Okayama University Medical School affil-num=2 en-affil= kn-affil=Department of Anesthesiology and Resuscitology, Okayama University Medical School affil-num=3 en-affil= kn-affil=Department of Anesthesiology and Resuscitology, Okayama University Medical School en-keyword=inhalational anesthetics kn-keyword=inhalational anesthetics en-keyword=muscle relaxants kn-keyword=muscle relaxants en-keyword=drug interaction kn-keyword=drug interaction en-keyword=neuromuscular transmission kn-keyword=neuromuscular transmission END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=6 article-no= start-page=321 end-page=323 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Successful Chemotherapy on a Pregnant Non-Hodgkin's Lymphoma Patient en-subtitle= kn-subtitle= en-abstract= kn-abstract=We report a case of a non-Hodgkin's lymphoma (NHL) patient treated successfully with combination chemotherapy during pregnancy who delivered a full-term baby. A 29 year-old patient with cervical and inguinal lymphadenopathy in the 27th week of gestation was referred to our hospital. The diagnosis of lymph node biopsy was NHL (diffuse, large cell type with B-cell phenotype). Three courses of CHOP regimen (adriamycin, cyclophosphamide, vincristine and prednisolone) were given before delivery. The patient has been in complete remission for three years and her baby has been in normal development. Our case supports previous reports that chemotherapy in the third trimester may be given safely on NHL patients.
en-copyright= kn-copyright= en-aut-name=TokiHironobu en-aut-sei=Toki en-aut-mei=Hironobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkabeHenichi en-aut-sei=Okabe en-aut-mei=Henichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KameiHaruto en-aut-sei=Kamei en-aut-mei=Haruto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShimokawaTatsuo en-aut-sei=Shimokawa en-aut-mei=Tatsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HiuraMasamichi en-aut-sei=Hiura en-aut-mei=Masamichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KondoMasashi en-aut-sei=Kondo en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HirotaYuichi en-aut-sei=Hirota en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Shikoku Cancer Center Hospital affil-num=2 en-affil= kn-affil=Shikoku Cancer Center Hospital affil-num=3 en-affil= kn-affil=Shikoku Cancer Center Hospital affil-num=4 en-affil= kn-affil=Shikoku Cancer cernter Hospital affil-num=5 en-affil= kn-affil=Shikoku Cancer Center Hospital affil-num=6 en-affil= kn-affil=Matsuyama Red Cross Hospital affil-num=7 en-affil= kn-affil=Matsuyama Red Cross Hospital en-keyword=non-Hodgkin's lymphoma kn-keyword=non-Hodgkin's lymphoma en-keyword=pregnancy kn-keyword=pregnancy en-keyword=chemotherapy kn-keyword=chemotherapy END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=6 article-no= start-page=325 end-page=328 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A case of bulbospinal muscular atrophy with chief complaint of sensory disorder in the lower extremities. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 56-year-old man was admitted to our department with a chief complaint of lower extremity dysesthesia. He described a dull numbness below the ankle and a dull pain in the nates for the past two years. Although the numbness extended to the thigh, he did not notice any muscular weakness or atrophy. Neurological examination revealed weakness and atrophy in the face, tongue and the proximal portions of all four extremities. Deep tendon reflexes were decreased. A moderate loss of vibratory sensation was noted below the knees. Electromyography showed neurogenic changes. Muscle biopsy revealed both myogenic and neurogenic changes. Sural nerve biopsy revealed a mild reduction of myelinated fibers, particularly the large-diameter fibers. Based on these findings, a diagnosis of bulbospinal muscular atrophy (BSMA) was made. In recent years, there have been some case reports of BSMA with sensory disturbances, or merely with subclinical manifestations of a sensory disturbance. This case is included in the same category as those reports, but it is interesting to note that the sensory disturbance in the lower extremities occurred as the chief complaint of the disease.
en-copyright= kn-copyright= en-aut-name=KurodaShigetoshi en-aut-sei=Kuroda en-aut-mei=Shigetoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UjikeHiroshi en-aut-sei=Ujike en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakashimaSachi en-aut-sei=Nakashima en-aut-mei=Sachi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OtsukiSaburo en-aut-sei=Otsuki en-aut-mei=Saburo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=bulbospinal muscular atrophy kn-keyword=bulbospinal muscular atrophy en-keyword=sensory disturbance kn-keyword=sensory disturbance END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=6 article-no= start-page=287 end-page=291 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of antioxidants on V79 Chinese hamster cells treated with ferric nitrilotriacetate. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The cytotoxic effects of ferric nitrilotriacetate (Fe-NTA) have been considered to be caused by free radicals produced by the drug. The present study was carried out to determine whether or not cytotoxic effects of Fe-NTA on cell growth and lipoperoxide formation of Chinese hamster cells were reduced by antioxidants. Using a spin trapping technique, we found that hydroxyl radical formation in the cells increased in the presence of Fe-NTA. Antioxidants, with the exception of superoxide dismutase, slightly inhibited production of the hydroxyl radical. Mannitol significantly reduced lipoperoxide formation, but other antioxidants did not. However, the growth inhibitory effects of Fe-NTA were not attenuated by these antioxidants. These results indicated that the cytotoxic effects of Fe-NTA may be mostly due to unknown factors other than oxygen free radicals.
en-copyright= kn-copyright= en-aut-name=TakeharaYoshiki en-aut-sei=Takehara en-aut-mei=Yoshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshiokaTamotsu en-aut-sei=Yoshioka en-aut-mei=Tamotsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NambaMasayoshi en-aut-sei=Namba en-aut-mei=Masayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Kurashiki Center for Adult Diseases affil-num=2 en-affil= kn-affil=Kurashiki Center for Adult Diseases affil-num=3 en-affil= kn-affil=Okayama University en-keyword=V79 cells kn-keyword=V79 cells en-keyword= ???NTA kn-keyword= ???NTA en-keyword=antioxidants kn-keyword=antioxidants en-keyword=cytotoxicity kn-keyword=cytotoxicity en-keyword=free radicals kn-keyword=free radicals END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=6 article-no= start-page=333 end-page=335 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Three-dimensional architecture of rat glomerular basement membrane by ultra-high resolution scanning electron microscopy. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We demonstrated the ultrastructure of rat glomerular basement membrane (GBM) by ultra-high resolution scanning electron microscopy. GBM prepared by sonication methods and conductive-staining could be observed without metal coating at magnifications as high as 400,000 times. The GBM showed an irregular meshwork structure composed of various strands and pores. The width of the strands ranged from 6 to 15 nm, and the diameter of pores ranged from 6 to 50 nm. The present study confirmed our molecular sieve theory of the basement membrane.
en-copyright= kn-copyright= en-aut-name=YamasakiYasushi en-aut-sei=Yamasaki en-aut-mei=Yasushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MakinoHirofumi en-aut-sei=Makino en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HironakaKazue en-aut-sei=Hironaka en-aut-mei=Kazue kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HayashiYoshikazu en-aut-sei=Hayashi en-aut-mei=Yoshikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShikataKenichi en-aut-sei=Shikata en-aut-mei=Kenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=glomerular basement membrance kn-keyword=glomerular basement membrance en-keyword=ultrastructure kn-keyword=ultrastructure en-keyword=ultra-high resolution scanning electron microscopy kn-keyword=ultra-high resolution scanning electron microscopy en-keyword=conductive staining kn-keyword=conductive staining END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=6 article-no= start-page=293 end-page=300 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Classification of Maximal Expiratory Flow-Volume Types Observed in Non-Smoking Healthy Young Subjects en-subtitle= kn-subtitle= en-abstract= kn-abstract=Pulmonary function tests were performed on 234 healthy non-smoking young subjects (189 males and 45 females free from respiratory and allergic symptoms). Maximal expiratory flow-volume (MEFV) curves were visually classified into five MEFV types: Type A, convex or straight flow changes; types B, C, and D, concave-convex-concave flow changes; and type E, sudden flow-fall and accompanying decreased flow rates at lower lung volumes. The reproducibility of MEFV patterns were shown by one way analysis of variance (ANOVA) of MEFV data obtained from 4 groups each consisting of 3-4 males and representing different MEFV types. Distribution of MEFV types was different between males and females; the rate of type A was higher in females than in males and those of types B and E were higher in males than in females. When analyzed in terms of three fractional flow rates, Fr-75, Fr-50, and Fr-25, these values could also be classified into 5 types similarly to the visual MEFV type analysis. It is concluded that MEFV type analysis is useful in assessing health conditions.
en-copyright= kn-copyright= en-aut-name=MeguroTadamichi en-aut-sei=Meguro en-aut-mei=Tadamichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=maximal expiratory flow-volume type kn-keyword=maximal expiratory flow-volume type en-keyword=sex difference kn-keyword=sex difference END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=6 article-no= start-page=301 end-page=308 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Increased ability of peripheral blood B cells from patients with rheumatoid arthritis to produce interleukin 1 in vitro. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Twenty-four patients with rheumatoid arthritis (RA) and 20 normal controls were examined for the ability of their peripheral blood B cells to produce interleukin 1 (IL-1) with or without lipopolysaccharide (LPS). B cells were purified from peripheral blood by negative selection methods (i.e., removal of adherent cells and sheep red blood cell rosette-forming cells, followed by treatment with monoclonal antibodies (OKT3 and OKM1) and complement). The amount of IL-1 in B cell culture supernatants (SN) was measured by thymocyte and fibroblast proliferation assays and an enzyme-linked immunosorbent assay for IL-1 alpha and beta. As a group, cultured B cells from patients with RA, both spontaneously and when stimulated with LPS, produced higher levels of IL-1 than those from normal controls. IL-1 production by RA B cells with LPS had a weak but positive correlation with disease activity. Moreover, RA B cell culture SN with elevated levels of IL-1 had a synergistic effect on the growth of anti-human IgM (anti-mu) stimulated B cells. In separate experiments, the growth of RA B cells was significantly promoted by IL-1 beta both with and without anti-mu stimulation. These results suggest that B cell-derived IL-1 may be involved in the B cell clonal expansion of RA through its own activity as a B cell stimulatory factor.
en-copyright= kn-copyright= en-aut-name=YamamuraMasahiro en-aut-sei=Yamamura en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishiyaNoji en-aut-sei=Nishiya en-aut-mei=Noji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=rheumatoid arthritis kn-keyword=rheumatoid arthritis en-keyword=B cells kn-keyword=B cells en-keyword=interleukin 1 kn-keyword=interleukin 1 en-keyword=B cell stimulatory factor kn-keyword=B cell stimulatory factor END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=6 article-no= start-page=417 end-page=422 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Changes in levels of arachidonic acid metabolites in blood and bronchoalveolar lavage fluid after warm ischemia-reperfusion of lung. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The purpose of this study was to evaluate the role of arachidonic acid metabolites in the reimplantation response after lung transplantation in mongrel dogs. The left lung was used and two groups were studied. Group I underwent hilar stripping, while Group II underwent hilar stripping plus warm ischemia for 60 min., achieved by clamping the left pulmonary artery and veins. We measured the lung wet to dry weight ratio (W/D ratio), total pulmonary vascular resistance (TPVR), and blood and bronchoalveolar lavage fluid (BALF) levels of leukotriene B4 and C4 (LTB4,C4) and thromboxane B2 (TXB2). These parameters were measured periodically for 7 days after reperfusion. In group II, the W/D ratio and TPVR were significantly increased in comparison with Group I. The blood LTC4 level was elevated immediately after reperfusion, and BALF level of LTC4 also rose subsequently. These levels changed concomitantly with the W/D ratio. The above results suggest that arachidonic acid metabolism plays an important role in the reimplantation response, especially in pulmonary edema.
en-copyright= kn-copyright= en-aut-name=ShimizuNobuyoshi en-aut-sei=Shimizu en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KitaTakashi en-aut-sei=Kita en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AoeMotoi en-aut-sei=Aoe en-aut-mei=Motoi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakataMasao en-aut-sei=Nakata en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyaiYoshiaki en-aut-sei=Miyai en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TeramotoShigeru en-aut-sei=Teramoto en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=lung transplantation kn-keyword=lung transplantation en-keyword=reperfusion kn-keyword=reperfusion en-keyword=arachidonic acids kn-keyword=arachidonic acids END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=6 article-no= start-page=329 end-page=331 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Increased accumulation of cytosine arabinoside in human leukemic cells and enhancement of its cell-killing activity by uridine. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of uridine(UR) on the cell-killing activity of cytosine arabinoside(ara-C) against human leukemic cells, MOLT-4, and on ara-C accumulation in cells were studied. The 50% lethal dose(LD50) of ara-C as determined by clonogenic assay was decreased to 5.0 x 10(-8) mol from 9.0 x 10(-7) mol after 3 days exposure to 10(-3) mol of UR. The accumulation of 3H-ara-C at 24 and 48 h was significantly increased in culture medium containing 10(-8) mol of 3H-ara-C and 10(-3) mol of UR (5,129 +/- 123.5 vs 2,554 +/- 115.5 cpm/10(5) cells at 24 h, p less than 0.01, and 5,772 +/- 123.2 vs 1,372 +/- 51.8 cpm/10(5) cells at 48 h, p less than 0.01). It is noteworthy that cell-killing activity of ara-C against human leukemic cells was enhanced by the combination with a nucleoside(UR), but not with antileukemic agents.
en-copyright= kn-copyright= en-aut-name=TakahashiIsao en-aut-sei=Takahashi en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HayashiNaoki en-aut-sei=Hayashi en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakamuraToru en-aut-sei=Nakamura en-aut-mei=Toru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MatuzakiToshiro en-aut-sei=Matuzaki en-aut-mei=Toshiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MuraseToshio en-aut-sei=Murase en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OsadaKen en-aut-sei=Osada en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakeuchiMakoto en-aut-sei=Takeuchi en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=UekiYasufumi en-aut-sei=Ueki en-aut-mei=Yasufumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NakadaHiroshi en-aut-sei=Nakada en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YorimitsuSeiichi en-aut-sei=Yorimitsu en-aut-mei=Seiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=10 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=11 en-affil= kn-affil=Kochi Municipal Central Hospital en-keyword=cytosine arabinosids kn-keyword=cytosine arabinosids en-keyword=uridine kn-keyword=uridine en-keyword=antileukemic effect kn-keyword=antileukemic effect en-keyword=accumulation of cytosine arabinoside kn-keyword=accumulation of cytosine arabinoside END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=6 article-no= start-page=309 end-page=314 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of selected splenic irradiation on growth of meth A-fibrosarcoma in mice and partial characterization of splenic effector and suppressor cell populations. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Meth A-fibrosarcoma bearing BALB/c mice were subjected to selected splenic irradiation (2.0-4.0 Gy) on days 7 and 14 of tumor growth. Tumor growth was recorded by serial measurement. Irradiation given on day 7 caused regression of tumor, but irradiation given on day 14 did not show tumor regression. Antitumor activity in the Winn assay was detected in spleen cells 3 days after irradiation, but was not detected 7 days after. The cell surface phenotypes were analyzed on days 3, 7 and 14 of splenic irradiation using monoclonal antibodies (anti-Thy1.2 antibody, anti-Lyt1 antibody, anti-Lyt2 antibody, anti-L3T4 antibody) by flow cytometry. Thy 1.2, Lyt1, and L3T4 cells were increased on day 3 of splenic irradiation, but were not on days 7 and 14. Lyt2-cells did not show increase on days 3, 7 and 14. It was possibly suggested that selected splenic irradiation induced tumor regression was caused by the ability of irradiation to preferentially eliminate suppressor T cells, thereby allowing effector T-cells to become relatively dominant.
en-copyright= kn-copyright= en-aut-name=SasakiAkinori en-aut-sei=Sasaki en-aut-mei=Akinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FuchimotoSadanori en-aut-sei=Fuchimoto en-aut-mei=Sadanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=selected splenic irradiation kn-keyword=selected splenic irradiation en-keyword=suppressor T-cell kn-keyword=suppressor T-cell en-keyword=flow cytometry kn-keyword=flow cytometry END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=2 article-no= start-page=93 end-page=102 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of posterior hypothalamic knife cuts on the baroreflex and hemorrhage-induced hormonal responses. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We made posterior hypothalamic knife cuts in rats to transect the fibers of the medial forebrain bundle (MFB) at the level of the mammillary body. The role of the MFB in the baroreflex and hemorrhage-induced hormonal responses was then examined in the unanesthetized, freely moving condition. The slopes for the relationship between changes in pulse interval and mean arterial pressure (MAP) in the posterior-cut group were significantly steeper than those in the sham-cut group both when there were phenylephrine-induced increases in MAP (1.13 +/- 0.07 vs 0.86 +/- 0.10 msec/mmHg) and nitroprusside-induced decreases in MAP (1.16 +/- 0.10 vs 0.77 +/- 0.05 msec/mmHg). This result indicates that posterior cuts elevated baroreflex sensitivity when MAP was increased or decreased. The resting MAP was not changed, but the resting heart rate (HR) was lowered by the posterior cuts. Furthermore, the posterior cuts augmented hypotensive hemorrhage-induced bradycardia. Hypotensive hemorrhage (16-17 ml/kg) caused elevation of the plasma catecholamine, ACTH and vasopressin (AVP) levels, but the posterior cuts attenuated these hormonal responses. These results indicate that the fibers in the MFB have a tonic inhibitory effect on the baroreflex in the resting condition, and play a stimulatory role in hemorrhage-induced catecholamine, ACTH and AVP responses.
en-copyright= kn-copyright= en-aut-name=MakinoShinya en-aut-sei=Makino en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HashimotoKozo en-aut-sei=Hashimoto en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=medial forebrain bundle kn-keyword=medial forebrain bundle en-keyword=baroreflex kn-keyword=baroreflex en-keyword=catecholamine kn-keyword=catecholamine en-keyword=vasopressin kn-keyword=vasopressin en-keyword=adrenocorticotropic hormone kn-keyword=adrenocorticotropic hormone END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=2 article-no= start-page=103 end-page=111 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Changes in the levels of lipoperoxide and antioxidant factors in human placenta during gestation. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The concentration of lipoperoxides in maternal blood increases as gestation progresses. The concentration in pregnant women at 40 weeks gestation is 1.6 times higher than in nonpregnant women. The concentration in the cord blood, however, is 70% lower than that in maternal blood. To study the role of placental tissue in the difference in the lipoperoxide concentration between the cord blood and maternal blood, we investigated the lipoperoxide concentration, antioxidant activities and in vitro lipoperoxide formation in placental tissue during pregnancy. The lipoperoxide concentration was 50% lower in placental tissue of 40 weeks gestation than in tissue of 5-11 weeks gestation. Catalase and superoxide dismutase activities in placental tissues increased as gestation progressed, while glutathione peroxidase activity and alpha-tocopherol concentration did not change significantly during the gestational period. The in vitro formation of lipoperoxides in placental tissue decreased as gestation progressed. These results show that placental tissue suppresses lipoperoxide formation in the late gestational age, lowers the concentration of lipoperoxides in the blood and protects the fetus against oxygen toxicity.
en-copyright= kn-copyright= en-aut-name=TakeharaYoshiki en-aut-sei=Takehara en-aut-mei=Yoshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshiokaTamotsu en-aut-sei=Yoshioka en-aut-mei=Tamotsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Center for Adult Diseases affil-num=2 en-affil= kn-affil=Center for Adult Diseases affil-num=3 en-affil= kn-affil=Okayama University en-keyword=lipoperoxides kn-keyword=lipoperoxides en-keyword=antioxidant factors kn-keyword=antioxidant factors en-keyword=placenta kn-keyword=placenta en-keyword=human kn-keyword=human en-keyword=gestation kn-keyword=gestation END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=2 article-no= start-page=65 end-page=70 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Visualization of sialoglycoproteins in polyacrylamide gels by acidic ninhydrin reaction. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A new method for staining sialoglycoproteins in polyacrylamide gel after disc electrophoresis is described. The method utilizes the reaction of sialic acids with an acidic ninhydrin reagent which yields a stable color with an absorption maximum at 470 nm. After electrophoresis, the polyacrylamide gel is placed in a test tube and heated with 5 ml of the acidic ninhydrin reagent for 10 min in a boiling water bath. Sialoglycoproteins are detected as brown bands. No additional procedure such as destaining is necessary. When 20 micrograms fetuin, a sialoglycoprotein, per gel is applied, the band remains visible for at least 2 h. Stained gel can be scanned with a gel scanner at 470 nm. When the stained gel was dried on a sheet of polypropylene filter, the color was stable for at least one month. The present method is superior to the method using Stains-all (3,3'-diethyl-9-methyl-4,5,4',5'-dibenzothiacarbocyanine) in specificity and simplicity for the detection of sialoglycoproteins.
en-copyright= kn-copyright= en-aut-name=YaoKenzabroh en-aut-sei=Yao en-aut-mei=Kenzabroh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UbukaToshihiko en-aut-sei=Ubuka en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MasuokaNoriyoshi en-aut-sei=Masuoka en-aut-mei=Noriyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KinutaMasahiro en-aut-sei=Kinuta en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhtaJun en-aut-sei=Ohta en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IshinoKazushi en-aut-sei=Ishino en-aut-mei=Kazushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=sialoglycoprotein kn-keyword=sialoglycoprotein en-keyword=polyacrylamide gel electrophoresis kn-keyword=polyacrylamide gel electrophoresis en-keyword=staining kn-keyword=staining en-keyword=acidic ninhydrin reaction kn-keyword=acidic ninhydrin reaction END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=2 article-no= start-page=71 end-page=77 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of laminin and collagen type I on the morphology and secretion of proteins in human hepatoblastoma and hepatoma cell lines. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of laminin (LAM) and collagen type I (C-I) on human hepatoblastoma (HuH-6) and hepatoma (HuH-7) cell lines were investigated. C-I was superior to LAM in supporting the attachment of the cells, especially of HuH-6, to plastic surfaces. No effect of LAM and C-I on cellular morphology was recognizable by phase contrast microscopy. By scanning electron microscopy (SEM), much more microvilli were found on the cell surface of HuH-6 on LAM substrate than on C-I substrate. In HuH-7 cells, however, these microvilli were rarely found on either LAM substrate or C-I substrate. The gel profile of the proteins secreted by HuH-6 and HuH-7 cells was not affected by the culture substrate except for the major band, though the amount of alpha-fetoprotein (AFP) secreted was larger when the cells were cultured on LAM substrate than on C-I substrate. These results indicate that the ability of LAM or C-I to enhance attachment is different from that to enhance AFP production or microvilli expression in HuH-6 cells and probably in HuH-7 cells.
en-copyright= kn-copyright= en-aut-name=TokiwaTakayoshi en-aut-sei=Tokiwa en-aut-mei=Takayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EndoAkira en-aut-sei=Endo en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatoJiro en-aut-sei=Sato en-aut-mei=Jiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=laminin kn-keyword=laminin en-keyword=collagen type I kn-keyword=collagen type I en-keyword=hepatoma cells kn-keyword=hepatoma cells en-keyword=scanning electron microscopy kn-keyword=scanning electron microscopy en-keyword=gel profile kn-keyword=gel profile END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=2 article-no= start-page=79 end-page=86 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Participation of the parasympathetic and sympathetic nerves in regulation of gallbladder motility in the dog. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The participation of the parasympathetic and sympathetic nerves in the canine gallbladder motility was examined. Efferent stimulation of the parasympathetic (vagus) and sympathetic (celiac) nerves caused contraction or inhibition of the neck, body and fundus of the gallbladder. The contractile response induced by vagus nerve stimulation was reduced by subthreshold efferent stimulation of the celiac nerve, while the inhibitory response was neither reduced nor enhanced by subthreshold efferent stimulation of the celiac nerve. The contractile and inhibitory response induced by celiac nerve stimulation was not reduced in the neck, body and fundus by subthreshold efferent stimulation of the vagus nerve. The contractile response to vagus nerve stimulation was reversed to a relaxant response by atropine administration, which was reduced or abolished by hexamethonium. It is suggested that the vagus nerve-induced contractile response in the canine gallbladder is modulated by sympathetic nerves presynaptically at the vagus nerve endings in the enteric ganglion, but the vagus nerve-induced relaxant response, which probably was induced by non-adrenergic non-cholinergic inhibitory neurons, is not modulated by the sympathetic nerves.
en-copyright= kn-copyright= en-aut-name=YamasatoTeruhiro en-aut-sei=Yamasato en-aut-mei=Teruhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakayamaSosogu en-aut-sei=Nakayama en-aut-mei=Sosogu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=gallbladder kn-keyword=gallbladder en-keyword=vagus nerve kn-keyword=vagus nerve en-keyword=celiac nerve kn-keyword=celiac nerve en-keyword=contractile response kn-keyword=contractile response en-keyword=relaxant response kn-keyword=relaxant response END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=2 article-no= start-page=55 end-page=64 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Formation of Sulfate from L-Cysteine in Rat Liver Mitochondria en-subtitle= kn-subtitle= en-abstract= kn-abstract=Formation of sulfate in rat liver mitochondria was studied. About 0.1 mumol of sulfate was formed in mitochondria from 1 g of liver in 60 min when 10 mM L-cysteine was used as the substrate. Addition of either 10 mM 2-oxoglutarate or 10 mM glutathione to this system increased sulfate formation 3 to 4 times. The addition of both 2-oxoglutarate and glutathione resulted in a 20-fold increase in sulfate formation. Sulfate formation in the presence of 5 mM L-cysteine was 58% of that with 10 mM L-cysteine. L-Cysteine-glutathione mixed disulfide was not a good substrate, indicating that this mixed disulfide was not an intermediate of sulfate formation in the present system. Incubation of 3-mercaptopyruvate with rat liver mitochondria also resulted in sulfate formation, and the addition of glutathione accelerated it. Formation of sulfite and thiosulfate was also detected. These results indicate that sulfate is produced in mitochondria, at least in part, from L-cysteine through the transamination pathway (3-mercaptopyruvate pathway).
en-copyright= kn-copyright= en-aut-name=UbukaToshihiko en-aut-sei=Ubuka en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YuasaShigeki en-aut-sei=Yuasa en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OhtaJun en-aut-sei=Ohta en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MasuokaNoriyoshi en-aut-sei=Masuoka en-aut-mei=Noriyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YaoKenzaburoh en-aut-sei=Yao en-aut-mei=Kenzaburoh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KinutaMasahiro en-aut-sei=Kinuta en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=sulfate formatioon kn-keyword=sulfate formatioon en-keyword=cysteine metabolism kn-keyword=cysteine metabolism en-keyword=glutathione kn-keyword=glutathione en-keyword=3-mercaptopyruvate kn-keyword=3-mercaptopyruvate en-keyword=mitochondria kn-keyword=mitochondria END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=2 article-no= start-page=113 end-page=115 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Regulation of bile duct motility by vagus and sympathetic nerves in the pigeon. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Effects of stimulation of the vagus and sympathetic nerves on bile duct peristalses were studied in pigeons anesthetized with urethane. Vagus stimulation increased the frequency of peristalses. Atropine, hexamethonium and tetrodotoxin abolished this excitatory effect. After atropine, inhibition of peristalses sensitive to tetrodotoxin was produced. Stimulation of sympathetic area in the spinal cord inhibited peristalses. Propranolol converted this effect into an excitatory one, which was abolished by phentolamine. The results suggest that vagal and sympathetic innervations of the bile duct in pigeons are similar to those of the sphincter of Oddi in mammalian species.
en-copyright= kn-copyright= en-aut-name=NeyaToshiaki en-aut-sei=Neya en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamasatoTeruhiro en-aut-sei=Yamasato en-aut-mei=Teruhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MizutaniMasatoshi en-aut-sei=Mizutani en-aut-mei=Masatoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakayamaSosogu en-aut-sei=Nakayama en-aut-mei=Sosogu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=biliary system kn-keyword=biliary system en-keyword=bile duct kn-keyword=bile duct en-keyword=motility kn-keyword=motility en-keyword=innervation kn-keyword=innervation en-keyword=pigeon kn-keyword=pigeon END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=2 article-no= start-page=87 end-page=91 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Slow hyperpolarizing action of tryptamine on myenteric neurons of the isolated guinea-pig ileum. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the present study, tryptamine produced a slow hyperpolarization in a few neurons other than a slow depolarization in myenteric neurons of the isolated guinea-pig ileum. Neither the adrenergic neuron blocker, guanethidine nor the 5-hydroxytryptamine uptake inhibitor, zimelidine, which can inhibit the release of 5-hydroxytryptamine from enteric neurites induced by tryptamine (M. Takaki et al. (1985) Neuroscience 16, 223-240), affected this slow hyperpolarization. Therefore, it was concluded that the slow hyperpolarization induced by tryptamine in myenteric neurons was not mediated via the release of 5-hydroxytryptamine or noradrenaline. It might be possible that the hyperpolarization was induced by a direct action of tryptamine on myenteric neurons per se.
en-copyright= kn-copyright= en-aut-name=TakakiMiyako en-aut-sei=Takaki en-aut-mei=Miyako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MizutaniMasatoshi en-aut-sei=Mizutani en-aut-mei=Masatoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=JinJi-Guang en-aut-sei=Jin en-aut-mei=Ji-Guang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakayamaSosogu en-aut-sei=Nakayama en-aut-mei=Sosogu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=intracelluar recording kn-keyword=intracelluar recording en-keyword=tryptamine kn-keyword=tryptamine en-keyword=hyperpolarization kn-keyword=hyperpolarization en-keyword=myenteric neurons kn-keyword=myenteric neurons END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=5 article-no= start-page=267 end-page=272 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Senile delirium with special reference to situational factors and recurrent delirium. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Factors initiating senile delirium were examined in 129 elderly inpatients (65 years or older). Sixty-eight patients were males and 61 females, with a mean age of 76.3 years. Delirium developed in most cases on the first two days of admission in the hospital, and the admission appeared to be a key factor precipitating delirium in about 30% of the patients. Delirium resolved or improved in 80% of the patients, but usually persisted in patients with dementia. Senile delirium tended to reappear repeatedly in patients whose episode of delirium lasted for more than 2 weeks, was associated with dementia, or had a prior history of delirium.
en-copyright= kn-copyright= en-aut-name=KurodaShigetoshi en-aut-sei=Kuroda en-aut-mei=Shigetoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshizuHideki en-aut-sei=Ishizu en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UjikeHiroshi en-aut-sei=Ujike en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OtsukiSaburo en-aut-sei=Otsuki en-aut-mei=Saburo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MitsunobuKatsusuke en-aut-sei=Mitsunobu en-aut-mei=Katsusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ChudaMasaki en-aut-sei=Chuda en-aut-mei=Masaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YamamotoMitsutoshi en-aut-sei=Yamamoto en-aut-mei=Mitsutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama Saiseikai General Hospital affil-num=6 en-affil= kn-affil=Okayama Red Cross General Hospital affil-num=7 en-affil= kn-affil=Kagawa Prefectural Hospital en-keyword=delirium kn-keyword=delirium en-keyword=the elderly kn-keyword=the elderly en-keyword=hospitalization kn-keyword=hospitalization en-keyword=recurrent delirium kn-keyword=recurrent delirium END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=5 article-no= start-page=273 end-page=277 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Duodenal mucosal hemodynamics in patients with liver cirrhosis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Clinical studies show that patients with liver cirrhosis associated with portal hypertension have a high incidence of duodenal ulcer and duodenitis. However, little information is available concerning pathophysiological process of such duodenal diseases in liver cirrhosis. Hemodynamics of the duodenal mucosa was studied in cirrhotics with esophageal varices (68 cases) and in noncirrhotics with non-ulcer dyspepsia (37 cases) as well. In each group, hemoglobin concentration in the peripheral venous blood was measured, and mucosal hemodynamics was examined in 4 regions of the duodenum by endoscopic reflectance spectrophotometer. No significant intergroup difference was noted in the mean age or sex ratio. Hemoglobin concentration in the peripheral venous blood was significantly lower (p less than 0.01) in the cirrhotics. There were no significant intergroup differences in duodenal mucosal blood volume. However, the cirrhotics showed significantly lower oxygen saturation of hemoglobin in all regions of the duodenum (p less than 0.01). These results show that the cirrhotics with esophageal varices had relative increase in blood volume and decrease in oxygen saturation of hemoglobin in the duodenal mucosa. Such microcirculatory disturbances seem to predispose liver cirrhosis patients to duodenal injury.
en-copyright= kn-copyright= en-aut-name=TanakaMichio en-aut-sei=Tanaka en-aut-mei=Michio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InatsuchiShuji en-aut-sei=Inatsuchi en-aut-mei=Shuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TerasakiTeiichi en-aut-sei=Terasaki en-aut-mei=Teiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FunakiJun en-aut-sei=Funaki en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=BandouTsuyosi en-aut-sei=Bandou en-aut-mei=Tsuyosi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShimadaKazuhiko en-aut-sei=Shimada en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=WakabayashiHiroyuki en-aut-sei=Wakabayashi en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KubotaYoshiki en-aut-sei=Kubota en-aut-mei=Yoshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Toyama Medical and Pharmaceutical University affil-num=2 en-affil= kn-affil=Toyama Medical and Pharmaceutical University affil-num=3 en-affil= kn-affil=Toyama Medical and Pharmaceutical University affil-num=4 en-affil= kn-affil=Toyama Medical and Pharmaceutical University affil-num=5 en-affil= kn-affil=Toyama Medical and Pharmaceutical University affil-num=6 en-affil= kn-affil=Toyama Medical and Pharmaceutical University affil-num=7 en-affil= kn-affil=Toyama Medical and Pharmaceutical University affil-num=8 en-affil= kn-affil=Toyama Medical and Pharmaceutical University affil-num=9 en-affil= kn-affil=Toyama Medical and Pharmaceutical University en-keyword=liver cirrhosis kn-keyword=liver cirrhosis en-keyword=portal hypertension kn-keyword=portal hypertension en-keyword=duodenal mucosa kn-keyword=duodenal mucosa en-keyword=reflectance spectrophotometry kn-keyword=reflectance spectrophotometry en-keyword=duodenoscopy kn-keyword=duodenoscopy END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=5 article-no= start-page=243 end-page=250 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Comparison of monoclonal antibodies reactive with lymphocyte subsets in routinely fixed paraffin-embedded material: flow cytometric analyses, immunoperoxidase staining and influence of fixatives. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We have attempted to clarify the characteristics of monoclonal antibodies (MAbs) detecting lymphocyte subsets in fixed materials. We examined by means of flow cytometric technique influences of fixatives and reactivity with malignant lymphomas (MLs). Specific markers for T-cells were UCHL1 and OPD4, which reacted especially with helper/inducer T-cells. MT1 recognized almost all of T-cells from peripheral blood and tonsils, but reacted with a part of B-MLs. As for B-cell markers, L26 was the most reliable marker for B-MLs. L26 and MB1 antigens could not be detected on living cells flow cytometrically. LN1 reacted with a part of T-cells as well as B-cells, but fluorescent intensity of the former was apparently stronger than that of the latter. Although LN2 antigen was located mainly in the cytoplasm close to the nuclear membrane immunohistochemically, it could be detected on living cells flow cytometrically. LN2 positive cells belonged to B-cells in peripheral blood and tonsils. When fixed for relatively short time, B5 and buffered formalin were better for examining MAbs than non-buffered formalin and ethanol.
en-copyright= kn-copyright= en-aut-name=YoshinoTadashi en-aut-sei=Yoshino en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HoshidaYoshihiko en-aut-sei=Hoshida en-aut-mei=Yoshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MurakamiIchiro en-aut-sei=Murakami en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=AkagiTadaatsu en-aut-sei=Akagi en-aut-mei=Tadaatsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=monoclonal antibodies kn-keyword=monoclonal antibodies en-keyword=lymphocyte subset kn-keyword=lymphocyte subset en-keyword=flow cytometry kn-keyword=flow cytometry END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=5 article-no= start-page=283 end-page=286 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A variant Philadelphia chromosome (Ph1) positive chronic myelocytic leukemia. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A rare case of variant Philadelphia (Ph1) chromosome positive [46, XX, t (9; 22) (q34; q11), inv (9) (9q22; 22q13)] chronic myelocytic leukemia (CML) was described. The patient, 73 years old female, was hospitalized to our hospital because of leukocytosis. Hematological findings corresponded to those of CMLs. However, this case lacked hepatosplenomegaly. Southern blot analysis using a 3 breakpoint cluster region (bcr) probe revealed a bcr rearrangement. The patient has been in the chronic phase for sixteen months without treatment. Clinical and chromosomal changes are under observation in order to get accumulate data for a pathophysiological analysis of variant Ph1 positive CMLs.
en-copyright= kn-copyright= en-aut-name=HarutaYuro en-aut-sei=Haruta en-aut-mei=Yuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakahashiIsao en-aut-sei=Takahashi en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SekitoNoriko en-aut-sei=Sekito en-aut-mei=Noriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyamotoKanji en-aut-sei=Miyamoto en-aut-mei=Kanji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShimamotoMasaaki en-aut-sei=Shimamoto en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=WakitaYoshiharu en-aut-sei=Wakita en-aut-mei=Yoshiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KikkawaKiyoshi en-aut-sei=Kikkawa en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakamuraToru en-aut-sei=Nakamura en-aut-mei=Toru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=SetoTakumi en-aut-sei=Seto en-aut-mei=Takumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YamashitaJiro en-aut-sei=Yamashita en-aut-mei=Jiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=YorimiysuSeiichi en-aut-sei=Yorimiysu en-aut-mei=Seiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=MiyakeSusumu en-aut-sei=Miyake en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=MachidaKen-ichi en-aut-sei=Machida en-aut-mei=Ken-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= affil-num=1 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=2 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Shimamoto Hospital affil-num=6 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=7 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=8 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=9 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=10 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=11 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=12 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=13 en-affil= kn-affil=Kochi Municipal Central Hospital affil-num=14 en-affil= kn-affil=Okayama University en-keyword=variant Ph1 positive chronic myelocytic leukemia kn-keyword=variant Ph1 positive chronic myelocytic leukemia en-keyword=bcr rearrangement kn-keyword=bcr rearrangement END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=5 article-no= start-page=251 end-page=256 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Exhaled metallic mercury in acatalasemic, hypocatalasemic and normal mice injected with mercury (II) chloride. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the relationship between the catalase activity in mouse organs and the amounts of metallic mercury exhaled, normal, homozygous hypocatalasemic and acatalasemic mice were injected with mercuric chloride. The cumulative amount of metallic mercury exhaled by mice was evidently expressed in the descending order of acatalasemic, hypocatalasemic, and normal mice. Statistically significant differences in the cumulative exhaled metallic mercury levels were observed between acatalasemic and hypocatalasemic mice, between normal and hypocatalasemic mice, and between acatalasemic and normal mice using the method of one way analysis of variance (ANOVA). A linear relationship was obtained through logarithm of catalase activity in the lungs or the blood, and logarithm of the cumulative amount of the exhaled mercury.
en-copyright= kn-copyright= en-aut-name=MeguroTadamichi en-aut-sei=Meguro en-aut-mei=Tadamichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshiiKunihiko en-aut-sei=Ishii en-aut-mei=Kunihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univresity en-keyword=catalase kn-keyword=catalase en-keyword=metallic mercury kn-keyword=metallic mercury en-keyword=acatalasemic mice kn-keyword=acatalasemic mice en-keyword=hypocatalasemic mice kn-keyword=hypocatalasemic mice en-keyword=exhalation kn-keyword=exhalation END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=5 article-no= start-page=257 end-page=261 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Electrical behavior of myenteric neurons induced by mesenteric nerve stimulation in the guinea pig ileum. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Effects of mesenteric nerve (MN) stimulation on the electrophysiological behavior of myenteric neurons in the guinea pig ileum were investigated with intracellular recording techniques in the myenteric flaps innervated with mesenteric nerves. MN stimulation at 0.11-6 Hz evoked fast excitatory postsynaptic potentials (EPSPs) in 6 myenteric neurons (2 Type 2/AH, 3 NS and 1 Type 1/S cells) and rarely evoked antidromic soma spike potentials in 3 myenteric neurons. Fast EPSPs were abolished by hexamethonium. Slow EPSPs evoked by MN stimulation (Takaki and Nakayama (1988) Brain Res., 442, 351-353) were also obtained in 5 Type 2/AH neurons and were irreversibly abolished by superfusion with capsaicin 10 microM. It is, therefore, likely that fast EPSPs mediated by nicotinic cholinergic receptors are due to stimulation of the vagus nerve and slow EPSPs are mediated by a release of substance P at axosomatic synapses due to antidromic activation of the capsaicin-sensitive sensory nerves.
en-copyright= kn-copyright= en-aut-name=TakakiMiyako en-aut-sei=Takaki en-aut-mei=Miyako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakayamaSosogu en-aut-sei=Nakayama en-aut-mei=Sosogu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=autonomic ganglion kn-keyword=autonomic ganglion en-keyword=intestine kn-keyword=intestine en-keyword=mesenteric nerve (MN) stimulation kn-keyword=mesenteric nerve (MN) stimulation en-keyword=myenteric neuron kn-keyword=myenteric neuron END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=5 article-no= start-page=233 end-page=241 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of Hyperosmotic Stimulation and Adrenalectomy on Vasopressin mRNA Levels in the Paraventricular and Supraoptic Nuclei of the Hypothalamus: en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of salt loading and adrenalectomy on arginine vasopressin (AVP) mRNA levels in the paraventricular nucleus (PVN) and the supraoptic nucleus (SON) of the hypothalamus were studied by semiquantitative in situ hybridization histochemistry, using a synthetic oligonucleotide probe and a computer-assisted image analysis system. Salt loading (2% NaCl) for 7 days produced marked increases in AVP mRNA levels in the magnocellular neurons of the PVN, SON, and accessory nuclei. Adrenalectomy caused an increase in AVP mRNA expression in the magnocellular part of the PVN and the expansion of hybridization signals into its medial parvocellular region, where the cell bodies of corticotropin-releasing hormone (CRH) neurons are located. No apparent alteration of AVP mRNA levels was observed in the SON following adrenalectomy. These results indicate that hyperosmotic stimulation and the loss of circulating glucocorticoids had differential effects on AVP gene expression in the PVN and SON, and that the magnocellular PVN and SON neurons responded in different manners to the loss of feedback signals.
en-copyright= kn-copyright= en-aut-name=SuemaruShuso en-aut-sei=Suemaru en-aut-mei=Shuso kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HashimotoKozo en-aut-sei=Hashimoto en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OgasaTakashi en-aut-sei=Ogasa en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakaoToshihiro en-aut-sei=Takao en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HirakawaMakoto en-aut-sei=Hirakawa en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KawataMitsuhiro en-aut-sei=Kawata en-aut-mei=Mitsuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Kyoto Prefectural University of Madicine affil-num=7 en-affil= kn-affil=Okayama University en-keyword=in situ hybridization kn-keyword=in situ hybridization en-keyword=arginine vasopressin mRNA kn-keyword=arginine vasopressin mRNA en-keyword=hypothalamus kn-keyword=hypothalamus en-keyword=osmotic stimulation kn-keyword=osmotic stimulation en-keyword=adrenalectomy kn-keyword=adrenalectomy END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=5 article-no= start-page=279 end-page=282 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Primary non-Hodgkin's lymphoma of the rectum: a case report. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A rare gastrointestinal tract neoplasm, primary non-Hodgkin's B-cell lymphoma in a 39-year-old, asymptomatic woman is described. The tumor was originally localized in the rectum without evidence of any other lymphoma-involved organ and treated by curative surgical procedure associated with postoperative chemotherapy.
en-copyright= kn-copyright= en-aut-name=MoreiraLuis Fernando en-aut-sei=Moreira en-aut-mei=Luis Fernando kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeKazuhiko en-aut-sei=Watanabe en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YoshinoTadashi en-aut-sei=Yoshino en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FuchimotoSadanori en-aut-sei=Fuchimoto en-aut-mei=Sadanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=primary lymphoma kn-keyword=primary lymphoma en-keyword=rectum kn-keyword=rectum en-keyword=surgical treatment kn-keyword=surgical treatment END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=5 article-no= start-page=263 end-page=266 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Changes in the concentrations of urinary proteins after physical exercise. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The influence of physical exercise on the urinary excretion of proteins was examined in 17 male high school baseball players. Their urine was collected before and after exercise to determine the concentrations of total protein, albumin, beta 2-microglobulin and creatinine along with the activity of N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30). Concentrations of total protein, albumin, beta 2-microglobulin and creatinine increased significantly (p less than 0.01) after exercise, while N-acetyl-beta-D-glucosaminidase activity did not increase. Similar results were obtained when the concentrations of these urinary components were calculated on the basis of a urinary density of 1.024, and when they were expressed relative to the amount of creatinine. Positive correlations were seen among total protein, albumin, beta 2-microglobulin and creatinine concentrations, but not between the beta 2-microglobulin concentration and N-acetyl-beta-D-glucosaminidase activity. Isoenzyme activities of N-acetyl-beta-D-glucosaminidase in the urine were determined by electrophoresis on cellulose acetate plates. After exercise, the A-form increased slightly, and the B-form decreased slightly, but these changes were not statistically significant.
en-copyright= kn-copyright= en-aut-name=MiyaiTaizo en-aut-sei=Miyai en-aut-mei=Taizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=urinary protein kn-keyword=urinary protein en-keyword= ?2-microglobulin kn-keyword= ?2-microglobulin en-keyword=N-acetyl-?-D-glucosaminidase kn-keyword=N-acetyl-?-D-glucosaminidase en-keyword=isoenzyme kn-keyword=isoenzyme END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=3 article-no= start-page=141 end-page=147 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Combined anterior pituitary function test using CRH, GRH, LH-RH, TRH and vasopressin in patients with non-functioning pituitary tumors. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We examined 8 normal subjects and 16 patients with non-functioning pituitary tumors with a combined anterior pituitary test to evaluate the clinical usefulness of the test. Diagnoses included 9 of chromophobe adenoma, 3 of craniopharyngioma, 2 of Rathke's cleft cyst, and 1 each of intrasellar cyst and tuberculum sella meningioma. All subjects received hypothalamic releasing hormones: 1 micrograms/kg corticotropin releasing hormone (CRH), 1 micrograms/kg growth hormone releasing hormone (GRH), 500 micrograms thyrotropin-releasing hormone (TRH), 100 micrograms luteinizing hormone releasing hormone (LH-RH), and a relatively small dose (5 mU/kg) of lysine vasopressin (LVP). In the normal subjects, the addition of LVP potentiated the secretion of adenocorticotropic hormone (ACTH) induced by CRH, but had no significant effect on the secretion of other anterior pituitary hormones. In the combined test with 5 releasing hormones, the plasma ACTH and cortisol responses were not impaired in the majority of the patients before pituitary surgery. Serum thyroid-stimulating hormone (TSH), prolactin (PRL) and follicle-stimulating hormone (FSH) responses were not impaired in 82%, 70% and 67% of the patients, respectively, while the serum LH and GH responses were impaired in 67% and 73% of the patients, respectively. Following pituitary surgery, responses of these hormones to combined testing were similarly impaired in more than 75% of the patients. These results indicate that plasma ACTH, cortisol and serum TSH responses are fairly good before pituitary surgery but are impaired significantly after surgery. No subjects experienced any serious adverse effects related to the testing. These results suggest that combined testing with hypothalamic hormones is a convenient and useful method for evaluating pituitary function.
en-copyright= kn-copyright= en-aut-name=HashimotoKozo en-aut-sei=Hashimoto en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MakinoShinya en-aut-sei=Makino en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HirasawaRyuto en-aut-sei=Hirasawa en-aut-mei=Ryuto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakaoToshihiro en-aut-sei=Takao en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KageyamaJingo en-aut-sei=Kageyama en-aut-mei=Jingo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OgasaTakashi en-aut-sei=Ogasa en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=anterior pituitary function test kn-keyword=anterior pituitary function test en-keyword=pituitary tumors kn-keyword=pituitary tumors en-keyword=corticotropin releasing hormone kn-keyword=corticotropin releasing hormone en-keyword=growth hormone releasing hormone kn-keyword=growth hormone releasing hormone en-keyword=luteinzing hormone releasing hormone kn-keyword=luteinzing hormone releasing hormone en-keyword=thyrotropin releasing hormone kn-keyword=thyrotropin releasing hormone END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=3 article-no= start-page=149 end-page=153 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Bone marrow and spleen cell colony formation in mice infected with Schistosoma japonicum. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Progenitor cells in the bone marrow and the spleen of mice, whether infected with Schistosoma japonicum or not, formed cell clusters and colonies when incubated with culture supernatant fluid of spleen cells incubated with soluble egg antigen (SEA). The egg extract, up to a concentration of 250 micrograms/ml protein, did not directly stimulate progenitor cell proliferation in the bone marrow. Eosinophilia in mice infected with S. japonicum may be mediated indirectly by egg antigen-stimulated immune lymphocytes and not directly by the egg antigen.
en-copyright= kn-copyright= en-aut-name=IshiiAkira en-aut-sei=Ishii en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OwhashiMakoto en-aut-sei=Owhashi en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShimomuraHiroshi en-aut-sei=Shimomura en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AbeTatsuya en-aut-sei=Abe en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Miyazaki Medical College affil-num=4 en-affil= kn-affil=Okayama University en-keyword=Schistosoma japonicm kn-keyword=Schistosoma japonicm en-keyword=soluble egg antigen kn-keyword=soluble egg antigen en-keyword=colony stimulating factor kn-keyword=colony stimulating factor en-keyword=bone marrow kn-keyword=bone marrow en-keyword=spleen cell kn-keyword=spleen cell END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=3 article-no= start-page=123 end-page=128 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Tissue contents and urinary excretion of taurine after administration of L-cysteine and L-2-oxothiazolidine-4-carboxylate to rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=<p>Tissue contents and urinary excretion of taurine were studied in rats after the administration of L-cysteine and its derivatives. Average taurine content in the liver of rats fed a 25% casein diet for 7 days increased 2-fold 2h after the intraperitoneal administration of 5 mmol of L-cysteine per kg of body weight, whereas that in rats fed a 5% casein diet for 2 days increased only slightly. The difference in the liver taurine contents between these two groups was discussed in relation to cysteine dioxygenase. Taurine contents in the heart, brain and blood did not differ significantly between these two groups or between the control and the group of rats which received L-cysteine. The increase in liver taurine concentrations after L-cysteine administration was much higher than that after L-cystine administration, suggesting a difference in their absorption. The intraperitoneal administration of 5 mmol/kg of L-2-oxothiazolidine-4-carboxylate (OTCA) resulted in a 3-fold increase in liver taurine content. The average increase in taurine excretion in the 24-h urine after OTCA administration corresponded to about 6.0% and that in the next 24-h urine to about 2.6% of OTCA administered, suggesting that nearly 10% of OTCA was metabolized to taurine and excreted in the urine.</p>
en-copyright= kn-copyright= en-aut-name=TaguchiTazuko en-aut-sei=Taguchi en-aut-mei=Tazuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkagiReiko en-aut-sei=Akagi en-aut-mei=Reiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UbukaToshihiko en-aut-sei=Ubuka en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=taurine kn-keyword=taurine en-keyword=cysteine metabolism kn-keyword=cysteine metabolism en-keyword=2-oxothiazolidine-4-carboxyylate kn-keyword=2-oxothiazolidine-4-carboxyylate END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=3 article-no= start-page=129 end-page=133 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Intrinsic reflexes mediated via peptidergic neurons in the smooth muscle esophagus of the hen. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the extrinsically denervated smooth muscle esophagus of the hen anesthetized with urethane (1 g/kg, i. m.), it was studied whether peptidergic neurons in the intramural plexus are involved in the intrinsic reflex. Ascending and descending contractions, and descending relaxation were induced by electrical stimulation of a narrow segment of the esophagus. Naloxone (1 microM), desensitization to substance P (0.3 microM) and spantide (20 microM) inhibited the ascending and descending contractions, respectively. The degree of the inhibition of the contractile response by a combination of naloxone and substance P was nearly the same as that by a single administration of naloxone or substance P. The ascending and descending contractions were reduced to one-third of the control by hexamethonium (100 microM) and abolished by atropine (10 microM). The descending relaxation was abolished after desensitization to vasoactive intestinal peptide (0.3 microM). Taken together the results suggest that in the hen's esophagus, opioid- and substance P-containing neurons in the intramural plexus may act as preganglionic neurons of cholinergic motor neurons in the ascending and descending excitatory pathways and that vasoactive intestinal peptide-containing neurons are involved in the descending inhibitory pathway.
en-copyright= kn-copyright= en-aut-name=NeyaToshiaki en-aut-sei=Neya en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsuoShinji en-aut-sei=Matsuo en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakayamaSosogu en-aut-sei=Nakayama en-aut-mei=Sosogu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=intrinsic reflex kn-keyword=intrinsic reflex en-keyword=peptidergic neuron kn-keyword=peptidergic neuron en-keyword=opioid kn-keyword=opioid en-keyword=substance P kn-keyword=substance P en-keyword=hen's esophagus kn-keyword=hen's esophagus END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=3 article-no= start-page=117 end-page=122 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Excretion of 3-Mercaptolactate-Cysteine Disulfide, Sulfate and Taurine in human Urine before and after Oral Administration of Sulfur-containing Amino Acids. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The excretion of 3-mercaptolactate-cysteine mixed disulfide [S-(2-hydroxy-2-carboxyethylthio)-L-cysteine, HCETC], sulfate and taurine in the urine of normal adults was investigated before and after oral administration of L-cysteine and related sulfur-containing amino acids. Before the loading of amino acids, the excretion (mean +/- SD) per kg of body weight per day of HCETC, free sulfate and taurine was 0.096 +/- 0.042, 305.7 +/- 66.1 and 31.9 +/- 8.7 mumols, respectively. After the loading of L-cysteine (800 mumols/kg of body weight), the average excretion in the 24-h urine of HCETC increased 2-fold and that of taurine increased 1.6-fold. The average excretion of free sulfate after the L-cysteine loading was 989.4 +/- 145.1 and 388.8 +/- 51.6 mumols/kg per day in the first and second 24-h urine, respectively, indicating that the sulfur corresponding to 85% of the L-cysteine loaded was excreted as free sulfate in 24 h. Administration of L-cystine (400 mumols/kg) resulted in similar results. The increase in HCETC after L-cysteine or L-cystine administration indicates that L-cysteine is metabolized in part through the transamination pathway (3-mercaptopyruvate pathway) and that an equilibrium exists between the intake and excretion of sulfur in humans.
en-copyright= kn-copyright= en-aut-name=YuasaShigeki en-aut-sei=Yuasa en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkagiReiko en-aut-sei=Akagi en-aut-mei=Reiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UbukaToshihiko en-aut-sei=Ubuka en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MasuokaNoriyoshi en-aut-sei=Masuoka en-aut-mei=Noriyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YaoKenzaburoh en-aut-sei=Yao en-aut-mei=Kenzaburoh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=3-mercaptolactate-cysteine mixed disulfide kn-keyword=3-mercaptolactate-cysteine mixed disulfide en-keyword=cysteine matabolism kn-keyword=cysteine matabolism en-keyword=3-mercaptopyruvate pathway kn-keyword=3-mercaptopyruvate pathway en-keyword=sulfur amino acid kn-keyword=sulfur amino acid en-keyword=sulfate excretion kn-keyword=sulfate excretion END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=3 article-no= start-page=155 end-page=159 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A case of necrosis of hepatocellular carcinoma and tumor thrombus in the portal vein induced by transcatheter arterial lipiodol chemoembolization. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A case of hepatocellular carcinoma is reported in which the main tumor, intrahepatic metastases and a tumor thrombus in the portal vein were necrotized completely after Lipiodol chemoembolization. In this case, the tumor thrombus seemed to act as a portal embolus. This phenomenon is interesting because Lipiodol chemoembolization alone usually can not necrotize intra- or extra-capsular invasion, intrahepatic metastasis or tumor thrombus in the portal vein. This case is considered to be suggestive of a possible therapy for hepatocellular carcinoma.
en-copyright= kn-copyright= en-aut-name=HamazakiKeisuke en-aut-sei=Hamazaki en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MimuraHisashi en-aut-sei=Mimura en-aut-mei=Hisashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyashimaTakanao en-aut-sei=Miyashima en-aut-mei=Takanao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=GouchiAkira en-aut-sei=Gouchi en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IrisawaMinoru en-aut-sei=Irisawa en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma en-keyword=tumor thrombus kn-keyword=tumor thrombus en-keyword=transcatheter arterial embolization kn-keyword=transcatheter arterial embolization END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=3 article-no= start-page=135 end-page=139 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Capsaicin-sensitive and hexamethonium-insensitive circular muscle responses to mesenteric nerve stimulation in the isolated guinea pig ileum. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Capsaicin-sensitive and hexamethonium-insensitive contractile and relaxatory motor responses of circular muscle induced by mesenteric nerve stimulation were studied. Desensitization to substance P or to neurokinin A, or the substance P antagonist, spantide largely reduced the initial contractile response. Desensitization to calcitonin gene-related peptide moderately reduced the late prolonged relaxation response. These results indicate that both responses of the circular muscle to mesenteric nerve stimulation may be attributed to a release of neuropeptides evoked by the 'efferent' stimulation of capsaicin-sensitive sensory nerves.
en-copyright= kn-copyright= en-aut-name=TakakiMiyako en-aut-sei=Takaki en-aut-mei=Miyako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=JinJi-Guang en-aut-sei=Jin en-aut-mei=Ji-Guang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakayamaSosogu en-aut-sei=Nakayama en-aut-mei=Sosogu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=calcitonin gene-relatsd peptide kn-keyword=calcitonin gene-relatsd peptide en-keyword=capsaicin kn-keyword=capsaicin en-keyword=ileal motility kn-keyword=ileal motility en-keyword=mesenteric nerve kn-keyword=mesenteric nerve en-keyword=substance P kn-keyword=substance P END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=41 end-page=45 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Bunina bodies in dendrites of patients with amyotrophic lateral sclerosis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We studied the brains of two cases of amyotrophic lateral sclerosis with dementia. Bunina bodies were found in the motor neurons of cranial nerve nuclei (trigeminal, facial and hypoglossal nerves) as well as in the spinal motoneurons. They appeared mostly in the cytoplasm and occasionally in the neuronal processes. However, the present electron microscopic study disclosed clearly that Bunina bodies were present not only in the cell body but also in the dendrites. No Bunina bodies were observed in the axons. It is inferred that the Bunina bodies were degenerative products formed as a result of a protein metabolism disorder.
en-copyright= kn-copyright= en-aut-name=KurodaShigetoshi en-aut-sei=Kuroda en-aut-mei=Shigetoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshizuHideki en-aut-sei=Ishizu en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawaiKensuke en-aut-sei=Kawai en-aut-mei=Kensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OtsukiSaburo en-aut-sei=Otsuki en-aut-mei=Saburo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=intradendritic Bunina body kn-keyword=intradendritic Bunina body en-keyword=amyotrophic lateral sclerosis kn-keyword=amyotrophic lateral sclerosis END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=47 end-page=50 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Determination of hypotaurine and taurine in blood plasma of rats after the administration of L-cysteine. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A method for the simultaneous determination of hypotaurine and taurine was developed. The method consisted of the elimination of urea, which interfered with the determination of hypotaurine, by immobilized urease, and determination of hypotaurine and taurine with an amino acid analyzer. The analyzer equipped with a cation-exchange column was operated at 32 degrees C with 0.2 M sodium citrate buffer, pH 2.8. Using this method, the dynamics of hypotaurine and taurine in blood plasma of rats was studied after the intraperitoneal injection of L-cysteine. The concentration of cysteine reached the maximum 1 h after L-cysteine loading. The concentration of hypotaurine and taurine increased in parallel and reached the maximum 2 h after L-cysteine loading. These changes seem to indicate the precursor-product relationship of these substances and the rapid conversion of hypotaurine to taurine in vivo.
en-copyright= kn-copyright= en-aut-name=YuasaShigeki en-aut-sei=Yuasa en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkagiReiko en-aut-sei=Akagi en-aut-mei=Reiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UbukaToshihiko en-aut-sei=Ubuka en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=hypotaurine kn-keyword=hypotaurine en-keyword=taurine kn-keyword=taurine en-keyword=determination kn-keyword=determination en-keyword=cysteine metabolisn kn-keyword=cysteine metabolisn en-keyword=amino acid analysis kn-keyword=amino acid analysis END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=9 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Selection of medium for serum-free primary culture of adult rat hepatocytes. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To select a suitable medium for serum-free primary culture of adult rat hepatocytes, ten commercially-available synthetic media were compared for their ability to maintain the cells under serum-free and serum-supplemented conditions with special reference to attachment, survival and albumin secretion. It was found that Williams' medium E and DM-160 medium were the best among the ten media for maintaining hepatocytes under serum-free conditions in primary culture.
en-copyright= kn-copyright= en-aut-name=MiyazakiMasahiro en-aut-sei=Miyazaki en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=BaiLiyan en-aut-sei=Bai en-aut-mei=Liyan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatoJiro en-aut-sei=Sato en-aut-mei=Jiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=adult rat hepatocytes kn-keyword=adult rat hepatocytes en-keyword=serum-free primary culture kn-keyword=serum-free primary culture en-keyword=maintenance kn-keyword=maintenance en-keyword=Williams' medium E kn-keyword=Williams' medium E en-keyword=DM-160 medium kn-keyword=DM-160 medium END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=1 end-page=8 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=In Vivo Analysis of Extracellular Proteins in Rat Brains with a Newly Developed Intracerebral Microdialysis Probe en-subtitle= kn-subtitle= en-abstract= kn-abstract=Peptides and proteins in the extracellular space in the central nervous system were investigated in vivo using an intracerebral microdialysis probe. The molecular cut-off of the hollow fiber which was used for the probe was approximately 100 kDa. We examined recovery rates of several compounds in vitro. The recovery rates of proteins and peptides were between 7-28%, with the exceptions of substance P and insulin-like growth factor I. The recovery rates of monoamines and their metabolites were 22-40%. In in vivo studies, two major proteins with apparent molecular weights of 62 kDa and 12 kDa, and several minor proteins (28 kDa, 43 kDa, 52 kDa and 70 kDa) were detected by SDS-polyacrylamide gel electrophoresis in the dialysate from a probe implanted in the striatum of anesthetized rats. These results suggest that the newly developed, intracerebral microdialysis probe might be useful for investigating the dynamic changes of peptides and proteins in the central nervous system.
en-copyright= kn-copyright= en-aut-name=NakamuraMitsuo en-aut-sei=Nakamura en-aut-mei=Mitsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ItanoToshifumi en-aut-sei=Itano en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamaguchiFuminori en-aut-sei=Yamaguchi en-aut-mei=Fuminori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MizobuchiMasayuki en-aut-sei=Mizobuchi en-aut-mei=Masayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TokudaMasaaki en-aut-sei=Tokuda en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MatsuiHideki en-aut-sei=Matsui en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=EtohSiji en-aut-sei=Etoh en-aut-mei=Siji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HosokawaKiyoshi en-aut-sei=Hosokawa en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OhmotoTakashi en-aut-sei=Ohmoto en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=HataseOsamu en-aut-sei=Hatase en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Kagawa Medical School affil-num=2 en-affil= kn-affil=Kagawa Medical School affil-num=3 en-affil= kn-affil=Kagawa Medical School affil-num=4 en-affil= kn-affil=Kagawa Medical School affil-num=5 en-affil= kn-affil=Kagawa Medical School affil-num=6 en-affil= kn-affil=Kagawa Medical School affil-num=7 en-affil= kn-affil=Kagawa Medical School affil-num=8 en-affil= kn-affil=Kagawa Medical School affil-num=9 en-affil= kn-affil=Kagawa Medical School affil-num=10 en-affil= kn-affil=Kagawa Medical School en-keyword=protein kn-keyword=protein en-keyword=peptide kn-keyword=peptide en-keyword=microdialysis kn-keyword=microdialysis en-keyword=extracellular space kn-keyword=extracellular space en-keyword=probe kn-keyword=probe END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=21 end-page=33 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ultrastructural Localization of Endogenous Peroxidase Activity in Hashunoto's Thyroiditis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ultrastructural localization and intensity of endogenous thyroid peroxidase (TPO) in Hashimoto's thyroiditis were examined in relation to the serum thyroid hormone level, thyroid-stimulating hormone (TSH) concentration and anti-thyroid autoantibody titer. In Hashimoto's thyroiditis, TPO activity on the microvilli of follicular cells was more intense than that of normal thyroid tissue, but the intensity of the intracytoplasmic peroxidase reaction was generally weaker than that of Graves' or normal thyroid tissue. Microvillar TPO reaction products were positive in all thyroid follicular cells in patients with increased TSH levels, but no TPO activity was observed on the microvilli of patients with normal or low TSH levels, irrespective of their histological type or serum anti-microsomal antibody titer. It is suggested that TPO activity on the surface of microvilli of thyroid follicular cells in Hashimoto's thyroid gland is modulated by thyrotropin but is not affected by anti-thyroid autoantibodies. en-copyright= kn-copyright= en-aut-name=YamamotoNobuharu en-aut-sei=Yamamoto en-aut-mei=Nobuharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SuzukiShinya en-aut-sei=Suzuki en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Third Department of Internal Medicine, Okayama University Medical School affil-num=2 en-affil= kn-affil=Third Department of Internal Medicine, Okayama University Medical School affil-num=3 en-affil= kn-affil=Third Department of Internal Medicine, Okayama University Medical School en-keyword=Hashimoto's thyroiditis kn-keyword=Hashimoto's thyroiditis en-keyword=thyroid peroxidase kn-keyword=thyroid peroxidase en-keyword=anti-thyroid microsome antibody kn-keyword=anti-thyroid microsome antibody en-keyword=thyroid-stimulating hormone kn-keyword=thyroid-stimulating hormone END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=13 end-page=20 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Role of endogenous prostaglandin E2 in interleukin 1 production by peripheral blood monocytes from patients with rheumatoid arthritis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We studied the effect of endogenous prostaglandin E2 (PGE2) on interleukin 1 (IL-1) production by peripheral blood monocytes from patients with rheumatoid arthritis (RA). IL-1 production by RA monocytes was not different from that of monocytes from normal controls, when the cells were either unstimulated or stimulated with lipopolysaccharide (LPS, 20 micrograms/ml), as measured by two different bioassays (thymocyte or fibroblast proliferation assay) and enzyme-linked immunosorbent assay. However, IL-1 production by LPS-stimulated monocytes from RA patients cultured in medium containing indomethacin, an inhibitor of PGE2 synthesis, was significantly greater than that of monocytes from normal controls. In addition, the levels of PGE2 in culture supernatants of unstimulated or LPS-stimulated monocytes from RA patients were higher than in culture supernatants of monocytes from normal controls. Moreover, the increase of in vitro IL-2 production by RA T cells stimulated by phytohemagglutinin (PHA) was observed when monocytes were removed from peripheral blood mononuclear cells. These results indicated that peripheral blood monocytes from RA patients could produce IL-1 in excess in vitro, but that in vivo IL-1 production by RA monocytes and IL-2 induction by RA T cells might be negatively regulated by endogenous PGE2.
en-copyright= kn-copyright= en-aut-name=YamamuraMasahiro en-aut-sei=Yamamura en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishiyaKoji en-aut-sei=Nishiya en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univresity en-keyword=rheumatoid arthritis kn-keyword=rheumatoid arthritis en-keyword=monocytes kn-keyword=monocytes en-keyword=interleulin 1 kn-keyword=interleulin 1 en-keyword=prostaglandin E2 kn-keyword=prostaglandin E2 en-keyword=interleukin 2 kn-keyword=interleukin 2 END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=51 end-page=53 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Influence of emotional stress on pharmacokinetics of isosorbide dinitrate intraperitoneally administered to rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The plasma level of isosorbide dinitrate intraperitoneally administered to rats stressed by foot-shock was almost the same as that in non-stressed control rats. However, levels of its metabolites, 5-isosorbide mononitrate and 2-isosorbide mononitrate, were lower in stressed rats than in non-stressed rats, suggesting that stress may influence the metabolism and/or excretion of the metabolites.
en-copyright= kn-copyright= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FurunoKatsushi en-aut-sei=Furuno en-aut-mei=Katsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ArakiYasunori en-aut-sei=Araki en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=isosorbird dinitrate kn-keyword=isosorbird dinitrate en-keyword=pharmacokinetics kn-keyword=pharmacokinetics en-keyword=emotional stress kn-keyword=emotional stress en-keyword=rats kn-keyword=rats END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=35 end-page=39 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of HR-592, a new derivative of indole, on conditioned behavior. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Effects of HR-592, a new derivative of indole, on conditioned avoidance and intracranial self-stimulation behavior were investigated in rats using a shuttle box and a Skinner box, respectively. The oral administration of HR-592 at doses of 3-10 mg/kg caused a dose-dependent suppression of the conditioned avoidance response. Even the escape response was slightly suppressed in the group administered 10 mg/kg of HR-592. The self-stimulation behavior was suppressed dose-dependently from 1 to 8 h after the administration of 6-10 mg/kg of HR-592. These results indicate that the action of HR-592 on conditioned avoidance response and intracranial self-stimulation behavior is similar to the action of neuroleptics.
en-copyright= kn-copyright= en-aut-name=IchimaruYasuyuki en-aut-sei=Ichimaru en-aut-mei=Yasuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ArakiYasunori en-aut-sei=Araki en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoriyamaMinehiro en-aut-sei=Moriyama en-aut-mei=Minehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univresity affil-num=4 en-affil= kn-affil=Okayama University en-keyword=HR-592 kn-keyword=HR-592 en-keyword=indole derivative kn-keyword=indole derivative en-keyword=avoidance kn-keyword=avoidance en-keyword=hypothalamic self-stimulation kn-keyword=hypothalamic self-stimulation en-keyword=rats kn-keyword=rats END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=4 article-no= start-page=219 end-page=222 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Pharmacokinetic Evaluation of Omeprazole Suspension Following Oral Administration in Rats: Effect of Neutralization of Gastric Acid en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to evaluate a clinical use of omeprazole suspension, we examined the pharmacokinetics of omeprazole after oral administration in rats. Although the administration of omeprazole suspension buffered by NaHCO3 solution did not produce a significant increase in the area under the concentration-time curve (AUC) value compared with non-buffered group, the administration of NaHCO3 buffer immediately after dosing of omeprazole suspension buffered by NaHCO3 caused a significant increase in the AUC value. These results suggest that the NaHCO3 treatment following the administration of omeprazole buffered suspension effectively decreased the degradation of the compound by gastric acid. Therefore, the successive administration of NaHCO3 solution after the omeprazole dosing seems to be a simple and useful method for the administration to patients who cannot receive tablets.
en-copyright= kn-copyright= en-aut-name=WatanabeKazuhide en-aut-sei=Watanabe en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsukaNaoyuki en-aut-sei=Matsuka en-aut-mei=Naoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FurunoKatushi en-aut-sei=Furuno en-aut-mei=Katushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=EtoKohei en-aut-sei=Eto en-aut-mei=Kohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=omeprazole kn-keyword=omeprazole en-keyword=suspension kn-keyword=suspension en-keyword=pharmacokinetics kn-keyword=pharmacokinetics en-keyword=rats kn-keyword=rats END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=4 article-no= start-page=223 end-page=225 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Changes in cellular ultrastructure induced by gamma-interferon in K562 cells may be prerequisite for apoptosis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We report here the time-course of electron microscopic changes induced by gamma-interferon (IFN-gamma) in the human erythromyeloid leukemia cell line K562. In K562 cells treated with IFN-gamma for 6h, the nuclei were polygonal in shape and microvilli were far more abundant on cell membranes compared with control K562 cells, and invaginations were often seen in the cell membranes. There was a reduction in the number of cell-membrane microvilli and an increase in the number of lysosomal bodies in the cytoplasm of K562 cells treated with IFN-gamma for 12h. After treatment with IFN-gamma for 24h, the cell membrane microvilli disappeared, large numbers of cellular organelles were observed, such as mitochondria and lysosomes, and the cytoplasm became electron-dense. Cytoplasmic vesicles and vacuoles were also observed. These vesicles may correspond to an intermediate step in the ultimate cellular disintegration associated with apoptosis caused by IFN-gamma.
en-copyright= kn-copyright= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MarutakaMasahito en-aut-sei=Marutaka en-aut-mei=Masahito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MizukawaKiminao en-aut-sei=Mizukawa en-aut-mei=Kiminao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KookaHideo en-aut-sei=Kooka en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=IFN-? kn-keyword=IFN-? en-keyword=cellular ultrastructure kn-keyword=cellular ultrastructure en-keyword=K562 kn-keyword=K562 END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=4 article-no= start-page=191 end-page=196 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Clinicopathology of Chondrosarcoma en-subtitle= kn-subtitle= en-abstract= kn-abstract=We conducted a clinicopathological analysis of chondrosarcomas in 17 patients treated in our institute. The 5- and 10-year overall survival rates of the patients were 72.3% and 61.9%, respectively. The significant prognostic factors were size and histologic grade of the tumor. Sex, age, location of the primary tumor, or the presence of a preceding exostosis did not affect the treatment results significantly. Chondrosarcomas of histologic grades I and II did not metastasize, while all grade III and dedifferentiated chondrosarcomas metastasized to the lung. The local recurrence rate depended on the surgical margin. Wide excision with an adequate surgical margin is important to achieve local control of the chondrosarcoma.
en-copyright= kn-copyright= en-aut-name=UchidaYoichiro en-aut-sei=Uchida en-aut-mei=Yoichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KawaiAkira en-aut-sei=Kawai en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaguchiKohji en-aut-sei=Taguchi en-aut-mei=Kohji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YokoiTadashi en-aut-sei=Yokoi en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=PuJian en-aut-sei=Pu en-aut-mei=Jian kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=chondrosarcoma kn-keyword=chondrosarcoma en-keyword=pathological grading kn-keyword=pathological grading en-keyword=prognostic factors kn-keyword=prognostic factors END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=4 article-no= start-page=197 end-page=201 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Superoxide Dismutase Activity in Cerebrospinal Fluid and Its Relation to Compression of the Lumbosacral Nerve Root en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the pathophysiology of lumbosacral radiculopathy, inflammation of the nerve root is of critical importance. Additionally, free radicals have been shown to be associated with some inflammatory process. This study was designed to investigate whether free radicals participate in the pathophysiology of nerve root involvement. We measured superoxide dismutase (SOD) activity in cerebrospinal fluid (CSF) of 31 patients with unilateral lumbosacral radiculopathy caused by a herniated disc using electron spin resonance (ESR) spectrometry. Then SOD activity was compared with the type of nerve root compression as seen on preoperative myelography. SOD activity in the normal control group was 7U/ml, while that in the hernia group remarkably decreased. The concentration gradient of SOD activity was different between central herniation and centrolateral herniation. Our findings indicate that free radicals are generated after nerve root compression. Under severe deficiency of SOD activity in CSF, serum SOD penetrates into CSF after further compression. In addition, SOD in CSF may play an important role in protecting against nerve root involvement.
en-copyright= kn-copyright= en-aut-name=TamadaToshinori en-aut-sei=Tamada en-aut-mei=Toshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MoriAkitane en-aut-sei=Mori en-aut-mei=Akitane kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy en-keyword=superoxide dismutase(SOD) kn-keyword=superoxide dismutase(SOD) en-keyword= cerebrospinal fluid(CSF) kn-keyword= cerebrospinal fluid(CSF) en-keyword=nerve root compression kn-keyword=nerve root compression en-keyword=inflammation kn-keyword=inflammation en-keyword=free radicals kn-keyword=free radicals END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=4 article-no= start-page=211 end-page=218 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Capillary Architecture in the Skeletal Muscles in the Rat Hind Limb en-subtitle= kn-subtitle= en-abstract= kn-abstract=We observed differences in the capillary architecture of the skeletal muscles that have different fiber metabolism. The soleus, the vastus intermedius and the tibialis anterior muscles of adult Wistar rats were prepared using two different techniques. Samples for adenosine triphosphatase (ATPase) staining were prepared following Dubovitz's method, and the distributions of fiber type, Types 1, 2A and 2B, were analyzed. Then, corrosion casts of capillary architecture of these muscles prepared following Murakami's method were observed with a scanning electron microscope (SEM) and compared with the fiber distribution. The fiber type composition of the soleus muscle showed Type 1 (slow-twitch) dominance and that of the vastus intermedius and the tibialis anterior muscle showed Type 2 (fast-twitch) dominance. The capillaries of the soleus muscle were tortuous, and this was thought to be advantageous for blood supply. In contrast, the capillaries of the vastus intermedius and tibialis anterior muscles had a relatively parallel pattern. Additionally, two different patterns of capillary architecture that appeared to correspond to certain metabolic characteristic of different muscle fiber types were preserved with corrosion casting. In conclusion, comparative studies on capillary architecture of the skeletal muscles are useful for analyses of its function.
en-copyright= kn-copyright= en-aut-name=TakaharaYasuhiro en-aut-sei=Takahara en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SendaMasuo en-aut-sei=Senda en-aut-mei=Masuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HashizumeHiroyuki en-aut-sei=Hashizume en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YagataYukihisa en-aut-sei=Yagata en-aut-mei=Yukihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=skeletal muscle kn-keyword=skeletal muscle en-keyword=ATPase staining kn-keyword=ATPase staining en-keyword=muscle fiber type kn-keyword=muscle fiber type en-keyword=corrosion cast kn-keyword=corrosion cast en-keyword=capillary architecture kn-keyword=capillary architecture END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=4 article-no= start-page=173 end-page=190 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Clinical applications of neurotransmitter-receptor studies in geriatric neuropharmacology. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The use of ligand-binding methods to study neurotransmitter-receptor sites has made its impact on almost all aspects of biological pursuits including research on aging and neurodegenerative diseases. In the past, most of the research in biochemical gerontology has largely centered around changes in various neurotransmitters and enzymatic activities. The molecular basis of aging and neurodegeneration at the level of neurotransmitter-receptor interactions has been highly appreciated in the last two decades as a result of receptor binding studies. It is now possible to obtain information about the regional distribution of neurotransmitter receptors in the brain, the pharmacological and biochemical characteristics of these sites, and the functional interrelationships between different neuronal systems in normal and pathological conditions. The passage of time after maturity is accompanied by measurable physiologic decline in virtually all systems. It is the aim of this work to discuss the practical aspects of neurotransmitter and/or drug (ligand)-receptor binding studies, highlighting some examples of their applications to geriatric neuropharmacology research, with special consideration to learning impairment and memory loss in normal and in pathological aging processes.
en-copyright= kn-copyright= en-aut-name=Gomez-VargasMarvin en-aut-sei=Gomez-Vargas en-aut-mei=Marvin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OgawaNorio en-aut-sei=Ogawa en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=aging kn-keyword=aging en-keyword=neurodegenerative diseases kn-keyword=neurodegenerative diseases en-keyword=neurotransmitters kn-keyword=neurotransmitters en-keyword=receptor binding kn-keyword=receptor binding en-keyword=neuropharmacology kn-keyword=neuropharmacology END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=4 article-no= start-page=203 end-page=210 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Platelet Taurine Concentration and Uptake in the Brattleboro Diabetes Insipidus Rat en-subtitle= kn-subtitle= en-abstract= kn-abstract=Since platelets accumulate taurine, they provide a model for studying the taurine transport in anisosmotic disorders. Thus, in this work we studied the taurine concentration and uptake in the platelets of Brattleboro rats, homozygous (DI) and heterozygous (HZ) for hereditary hypothalamic diabetes insipidus, and Long Evans (LE) normal rats after free water intake and after dehydration induced by water deprivation for 24 h. The decreased ability of the DI rats to concentrate urine led to plasma hypernatremia and hyperosmolality despite excessive drinking. Water deprivation in the DI rats induced drastic dehydration with exacerbated hypernatremia and hyperosmolality. Plasma hypernatremia and hyperosmolality resulted in a significant elevation of the taurine concentration and uptake by platelets of the DI rats. Kinetic assays showed that plasma hypernatremia and hyperosmolality did not alter the affinity of taurine to platelet membrane carrier, as expressed by Km, but caused a profound increase in the maximal transport capacity, Vmax. After free water intake the Vmax of the DI rats was about two times higher than that in the HZ and LE rats and after water deprivation it was about three times higher. Water deprivation doubled the Vmax of the DI rats without changing the Km.
en-copyright= kn-copyright= en-aut-name=NieminenMarja-Leena en-aut-sei=Nieminen en-aut-mei=Marja-Leena kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TuomistoLeena en-aut-sei=Tuomisto en-aut-mei=Leena kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SolatunturiErkka en-aut-sei=Solatunturi en-aut-mei=Erkka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=University of Helsinki affil-num=2 en-affil= kn-affil=University of Kuopio affil-num=3 en-affil= kn-affil=University of Kelsinki en-keyword=Brattleboro diabetes insipidus rat kn-keyword=Brattleboro diabetes insipidus rat en-keyword=platelet kn-keyword=platelet en-keyword=taurine concentrationm taurine uptake kn-keyword=taurine concentrationm taurine uptake en-keyword=osmoregulation kn-keyword=osmoregulation END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=6 article-no= start-page=319 end-page=324 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Immortalization of Cells from Brains Derived from a Strain (MSM/MSfB6C3F1) of Wild Mouse en-subtitle= kn-subtitle= en-abstract= kn-abstract=Escape from cellular aging is the rate-limiting step of multistep carcinogenesis. While normal human cells invariably undergo cellular aging and almost never spontaneously immortalize, cells derived from rodents such as mice are relatively easily immortalized. In this experiment, we studied the immortalization patterns of cells obtained from brain tissues of an inbred strain (MSM/MSfB6C3F1) derived from wild mice. We established 12 cell strains derived from 12 mouse brains in order to investigate whether these cells show cellular aging in the same fashion as human cells or whether these cells are immortalized as easily as rodent cells reported previously. As a result, all cell strains were immortalized up to about 200 days in culture. One strain immortalized very early, in the first 50 days, four strains immortalized in the last 200 days, and the other seven strains became immortal between 150 and 200 days in culture. All immortalized cell strains showed varying amounts of chromosome abnormalities, numerically and structurally, but no specific changes related to immortalization were detected. Before immortalization, three types of cells, glial-like, polygonal flat-thin, and fibroblast-like cells, were observed in culture, but after immortalization most of the cultures became fibroblastic. From these results, we concluded that fibroblast-like cells derived from brains of these mice immortalized in like fashion to fibroblasts of other inbred mice.
en-copyright= kn-copyright= en-aut-name=YuasaTakae en-aut-sei=Yuasa en-aut-mei=Takae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AbeEriko en-aut-sei=Abe en-aut-mei=Eriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimuraMitsuo en-aut-sei=Oshimura en-aut-mei=Mitsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NambaMasayoshi en-aut-sei=Namba en-aut-mei=Masayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Tottori University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=cells from mouse brains kn-keyword=cells from mouse brains en-keyword=immortalization kn-keyword=immortalization en-keyword=aging kn-keyword=aging en-keyword=chromosomes kn-keyword=chromosomes END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=6 article-no= start-page=325 end-page=333 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Relation between the instrumental activities of daily living and physical fitness tests in elderly women. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A cross-sectional study was conducted to quantitatively evaluate the relationship between the instrumental activities of daily living (IADL) and various physical fitness tests in elderly women living at home. The study focused on the total population of those women aged 65 years and over living in Y Town, Hyogo Prefecture, Japan, who visited a nursing home for day services. A total of 128 subjects were divided into two groups: dependent in IADL group (n = 49) and independent in IADL group (n = 79). The magnitude of the relation was evaluated by the odds ratio (OR). The following tests showed a significant decrease in IADL: knee-raising test [age-adjusted OR = 4.23, 95% confidence interval (CI) 1.81-9.87], height (age-adjusted OR = 4.09, 95% CI 1.75-9.56), grip strength (age-adjusted OR = 3.68, 95% CI 1.57-8.60), sit-and-reach test (age-adjusted OR = 2.76, 95% CI 1.20-6.34), and standing on one leg with closed eyes (age-adjusted OR = 2.56, 95% CI 1.09-5.97). Multivariate analysis using Hayashi's quantification method I indicated that knee-raising was the test most highly correlated with decreased IADL. These results suggest that measurement of knee-raising ability, muscle strength of the lower extremities and flexibility of hip joint could be the most useful factors to assess the level of instrumental self-support ability.
en-copyright= kn-copyright= en-aut-name=UchidaHayato en-aut-sei=Uchida en-aut-mei=Hayato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MinoYoshio en-aut-sei=Mino en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TsudaToshihide en-aut-sei=Tsuda en-aut-mei=Toshihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=BabazonoAkira en-aut-sei=Babazono en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawadaYuichi en-aut-sei=Kawada en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ArakiHidetoshi en-aut-sei=Araki en-aut-mei=Hidetoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OgawaTakanori en-aut-sei=Ogawa en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=AoyamaHideyasu en-aut-sei=Aoyama en-aut-mei=Hideyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University Medical School affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Kyusyu University en-keyword=elderly women living at home kn-keyword=elderly women living at home en-keyword=instrumental activities of daily living kn-keyword=instrumental activities of daily living en-keyword=physical fitness test kn-keyword=physical fitness test en-keyword=kneeraising ability kn-keyword=kneeraising ability END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=6 article-no= start-page=293 end-page=297 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Increased endothelial and epidermal thrombomodulin expression and plasma thrombomodulin level in progressive systemic sclerosis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the relation between systemic and cutaneous vascular endothelial injury in progressive systemic sclerosis (PSS), we examined thrombomodulin (TM) expression in PSS skin lesions immuno-histopathologically and compared it with plasma soluble TM levels measured by specific enzyme-linked immunosorbent assay. The plasma soluble TM level in PSS patients was significantly higher than that of normal controls and was as high as the levels of SLE patients. In relation to disease activities, the plasma TM levels of sclerotic phase PSS patients were significantly higher than that of atrophic phase PSS patients. The plasma samples with anti-Scl-70 antibody showed a high TM level than samples with anti-centromere antibody or anti-RNP antibody. Barnett's types or systemic corticosteroid treatment did not affect the TM level. Histopathologically, the dermal endothelial TM expression significantly increased in the sclerotic skin and moderately increased in the non-sclerotic skin of PSS compared with that of normal control skin. In addition, immunoreactive TM expression in the epidermis also increased in PSS. Disease activity-dependent elevation of plasma TM levels and immuno-histopathological expression of TM suggested generalized endothelial and epidermal cell involvement in PSS, and compensation in part by overproduction of TM by endothelial cells.
en-copyright= kn-copyright= en-aut-name=MizutaniHitoshi en-aut-sei=Mizutani en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HayashiTatsuya en-aut-sei=Hayashi en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NouchiNobuhiro en-aut-sei=Nouchi en-aut-mei=Nobuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=InachiShin en-aut-sei=Inachi en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SuzukiKoji en-aut-sei=Suzuki en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShimizuMasayuki en-aut-sei=Shimizu en-aut-mei=Masayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Mie University Faculty of Medicine affil-num=2 en-affil= kn-affil=Mie University Faculty of Medicine affil-num=3 en-affil= kn-affil=Mie University Faculty of Medicine affil-num=4 en-affil= kn-affil=Mie University Faculty of Medicine affil-num=5 en-affil= kn-affil=Mie University Faculty of Medicine affil-num=6 en-affil= kn-affil=Mie University Faculty of Medicine en-keyword=thrombomodulin kn-keyword=thrombomodulin en-keyword=scleroderma kn-keyword=scleroderma en-keyword=skin kn-keyword=skin en-keyword=endothelial cells kn-keyword=endothelial cells en-keyword=keratinocyte kn-keyword=keratinocyte END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=6 article-no= start-page=305 end-page=311 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cathepsin B in the Growth of Colorectal Cancer: Suppressive Effect of Leupeptin on the Growth of DMH-Induced Rat Colon Neoplasm en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cathepsin B, a thiol protease, has been reported to be involved in cancer progression and metastasis. The suppressive effects of two kinds of protease inhibitors, leupeptin and dietary camostate (FOY-305), on tumorigenesis and progression in 1, 2-dimethylhydrazine (DMH)-induced rat colon neoplasm were examined in relation to tissue cathepsin B activity. Male Donryu rats were treated with leupeptin or FOY-305 during or after the administration of DMH. There were no significant differences in average tumor numbers among all DMH-treated groups. However, the percentage of small tumors was significantly higher in the group in which leupeptin was supplied during DMH administration. This trend was not recognized in the FOY-305-treated groups. The ratio of cathepsin B activity in the tumors to that in the tumor-bearing tissue (T/Tb) was significantly increased with increasing tumor size (P = 0.009). The cathepsin B activity levels in the tumor-bearing mucosa in the groups which received leupeptin or FOY-305 following DMH treatment were both significantly lower than that in the group which received neither protease inhibitor (P = 0.046 and P = 0.0067, respectively). The results obtained indicate that leupeptin may have suppressed tumor growth by lowering the tissue cathepsin B activity.
en-copyright= kn-copyright= en-aut-name=SatohYasumasa en-aut-sei=Satoh en-aut-mei=Yasumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HigashiToshihiro en-aut-sei=Higashi en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NousoKazuhiro en-aut-sei=Nouso en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KinugasaNobuyuki en-aut-sei=Kinugasa en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YoshidaKeigo en-aut-sei=Yoshida en-aut-mei=Keigo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=UematsuShuji en-aut-sei=Uematsu en-aut-mei=Shuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakatsukasaHarushige en-aut-sei=Nakatsukasa en-aut-mei=Harushige kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NishimuraYukio en-aut-sei=Nishimura en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Kyushu University affil-num=10 en-affil= kn-affil=Okayama University en-keyword=cathepsin B kn-keyword=cathepsin B en-keyword=colorectal cancer kn-keyword=colorectal cancer en-keyword=colorectal adenoma kn-keyword=colorectal adenoma END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=6 article-no= start-page=285 end-page=292 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Continuous Measurement of Tissue Oxygen and Carbon Dioxide Gas Tensions in Dog Liver in Ischemia/Reperfusion en-subtitle= kn-subtitle= en-abstract= kn-abstract=An experiment was conducted to determine whether the oxygen and carbon dioxide gas tensions in liver tissue (PtO2 and PtCO2, respectively) reflect the state of microcirculation and/or metabolism in the ischemic liver. Subjects were divided into three groups: group 1, 30 min ischemia; group 2, 60 min ischemia; group 3, four times of intermittent 15 min ischemia after every 10 min of reperfusion. PtO2, PtCO2 and tissue blood flow (TBF) were measured by mass spectrometry, comparatively studied with the serum GOT level as an indicator of liver tissue damage. Furthermore, the time point at which the PtCO2 increase for 1 min initially became less than 1/2 of the maximum value was located on the transit curve of PtCO2, referred to as the critically anaerobic (CA) point, with which new indices of critically anaerobic score (CAS) and time (CAT) (see details in text) were developed. The profiles of PtO2 and PtCO2 during ischemia and reperfusion were clearly demonstrated, and the CA point was observed 12.7 +/- 2.9 min after induction of ischemia. PtO2 was positively correlated with TBF and negatively with the serum GOT level. Furthermore, not only CAS but also CAT were significantly correlated with PtO2, TBF, and the serum GOT level. It was concluded that PtCO2 reflects the state of anaerobic tissue metabolism during ischemia and PtO2 reflects the magnitude of microcirculatory disturbance and tissue injury caused by ischemia/reperfusion. Therefore, continuous monitoring of not only PtO2 but also PtCO2 is beneficial for patients undergoing hepatic surgery with ischemia. en-copyright= kn-copyright= en-aut-name=UrakamiAtsushi en-aut-sei=Urakami en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiraiRyuji en-aut-sei=Hirai en-aut-mei=Ryuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OtaTetsuya en-aut-sei=Ota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SogaHiroyuki en-aut-sei=Soga en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NawaSugato en-aut-sei=Nawa en-aut-mei=Sugato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShimizuNobuyoshi en-aut-sei=Shimizu en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Second Department of Surgery, Okayama University Medical School affil-num=2 en-affil= kn-affil=Second Department of Surgery, Okayama University Medical School affil-num=3 en-affil= kn-affil=Second Department of Surgery, Okayama University Medical School affil-num=4 en-affil= kn-affil=Second Department of Surgery, Okayama University Medical School affil-num=5 en-affil= kn-affil=Second Department of Surgery, Okayama University Medical School affil-num=6 en-affil= kn-affil=Second Department of Surgery, Okayama University Medical School en-keyword=liver kn-keyword=liver en-keyword=ischemia kn-keyword=ischemia en-keyword=oxygen kn-keyword=oxygen en-keyword=carbon dioxide kn-keyword=carbon dioxide en-keyword=mass spectrometry kn-keyword=mass spectrometry END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=6 article-no= start-page=305 end-page=311 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cathepsin B in the Growth of Colorectal Cancer: Increased Activity of Cathepsin B in Human Colorectal Cancer en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cathepsin B, a thiol protease, is involved in cancer metastasis. To clarify the role of cathepsin B in tumor progression in human colorectal cancer, the relationship between its activity, immunohistochemical staining, and clinical tumor progression was investigated. Cathepsin B activity in adenocarcinomas was significantly elevated compared with that in the tumor-bearing tissue. Furthermore, the tumor/tumor-bearing tissue (T/Tb) ratio of the activity was significantly higher than that of colorectal adenoma. Immunohistochemical studies demonstrated intense staining in the cancerous tissue. With respect to the clinical stage of tumors, the activity tended to be higher in tumors that had invaded the serosa or subserosa than in those that invaded the proper muscle. The results suggest that cathepsin B participates in the progression of human colorectal cancer, and its increased expression is a sensitive marker of the differentiation between colorectal adenoma and adenocarcinoma.
en-copyright= kn-copyright= en-aut-name=SatohYasumasa en-aut-sei=Satoh en-aut-mei=Yasumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HigashiToshihiro en-aut-sei=Higashi en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NousoKazuhiro en-aut-sei=Nouso en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KinugasaNobuyuki en-aut-sei=Kinugasa en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YoshidaKeigo en-aut-sei=Yoshida en-aut-mei=Keigo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=UematsuShuji en-aut-sei=Uematsu en-aut-mei=Shuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakatsukasaHarushige en-aut-sei=Nakatsukasa en-aut-mei=Harushige kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NishimuraYukio en-aut-sei=Nishimura en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama Univresity affil-num=9 en-affil= kn-affil=Kyushu University affil-num=10 en-affil= kn-affil=Okayama University en-keyword=cathepsin B kn-keyword=cathepsin B en-keyword=colorectal cancer kn-keyword=colorectal cancer en-keyword=colorectal adenoma kn-keyword=colorectal adenoma END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=6 article-no= start-page=313 end-page=317 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Perineuronal Sulfated Proteoglycans, Cell Surface Glycoproteins and Dark Neurons in the Cingulate Cortex of Newborn and Adult Rats en-subtitle= kn-subtitle= en-abstract= kn-abstract=Many neurons in the adult rat cingulate cortex possess perineuronal sulfated proteoglycans detectable with cationic iron colloid and aldehyde fuchsin, or cell surface glycoproteins reactive to lectin Vicia villosa or soybean agglutinin. The perineuronal sulfated proteoglycans develop three to four weeks after birth. The cell surface glycoproteins develop at earlier stage or two to three weeks after birth. Dark or active neurons begin to appear three to four weeks after birth. These findings indicate that the brain matures after birth or during weaning period.
en-copyright= kn-copyright= en-aut-name=TsubouchiMari en-aut-sei=Tsubouchi en-aut-mei=Mari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsubochiYutaka en-aut-sei=Tsubochi en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HitomiSayoko en-aut-sei=Hitomi en-aut-mei=Sayoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=rat brain kn-keyword=rat brain en-keyword=perineuronal sulfated proteoglycans kn-keyword=perineuronal sulfated proteoglycans en-keyword=cell surface glycoproteins kn-keyword=cell surface glycoproteins en-keyword=dark neurons kn-keyword=dark neurons END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=2 article-no= start-page=89 end-page=96 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199604 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Detection of Epstein-Barr virus RNA and related antigens in non-neoplastic lymphoid lesions. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To elucidate the latent state and reactivation of Epstein-Barr virus (EBV) in non-neoplastic lymphoid lesions, we investigated 144 non-neoplastic lymphoid lesions by in situ hybridization (ISH) to detect the expression of EBV-encoded small RNAs (EBER)-1 and BCRF-1 and by immunostaining for latent membrane protein (LMP)-1 and ZEBRA. ISH for EBER-1 detected EBER-1-positive cells (EPC) in 31 of the 144 examined lesions (22%). EPC were detected in 4 of 49 cases of nonspecific lymphoid hyperplasia, in 16 of 20 abscess-forming granulomatous lymphadenitis (AFGL), 5 of 25 Kikuchi's disease, and in 3 of 3 infectious mononucleosis. LMP-1 was expressed in 6 of 124 non-neoplastic lymphoid lesions (4.8%). LMP-1-positive cells were observed in 6 of the 31 EBER-1-positive cases (19%). EPC were detected significantly more frequently in LMP-1- and ZEBRA-positive specimens than in the LMP-1- and ZEBRA-negative specimens. BCRF-1 was expressed in 4 of 11 cases examined: 2 of 3 AFGL, 1 of 2 Kikuchi's disease, and in the 1 case of atypical lymphoid hyperplasia. This study suggests that Epstein-Barr virus is prevalent and can be reactivated in the lymph nodes effaced by destructive inflammation, such as AFGL. Such inflammation may provide a local milieu that is conducive for EBV to enter the lytic cycle.
en-copyright= kn-copyright= en-aut-name=TonoyamaYuji en-aut-sei=Tonoyama en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TeramotoNorihiro en-aut-sei=Teramoto en-aut-mei=Norihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SarkerAshit Baran en-aut-sei=Sarker en-aut-mei=Ashit Baran kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YoshinoTadashi en-aut-sei=Yoshino en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HayashiKazuhiko en-aut-sei=Hayashi en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AkagiTadaatsu en-aut-sei=Akagi en-aut-mei=Tadaatsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=EBER-I kn-keyword=EBER-I en-keyword=BCRF-l kn-keyword=BCRF-l en-keyword=LMP-l kn-keyword=LMP-l en-keyword=ZEBRA kn-keyword=ZEBRA en-keyword=lymphoid lesion kn-keyword=lymphoid lesion END