start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=387
end-page=393
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Partial purification and properties of Ca(2+)-binding protein from rat liver mitochondrial matrix.
kn-title=ラット肝ミトコンドリア・マトリクスのCa(2+)結合蛋白質の部分精製とその物性について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A 193-fold purification of Ca(2+)-binding protein from rat liver mitochondrial matrix was achieved. The Ca(2+)-binding protein consisted of 3 polypeptide subunits whose respective molecular weights by SDS polyacrylamide gel electrophoresis were 62K, 49K and 37K. The molecular weight of the protein was 150K to 220K. The Kd for Ca(2+) was 1.3×10-(5)M and lower for Mn(2+) and Mg(2+). The protein was inactivated by heat treatment at 100℃ for 1 min, though stable against treatment with 0.5% w/v trypsin at 37℃ for 30 min. Ruthenium red did not inhibit Ca(2+)-binding.
en-copyright=
kn-copyright=

en-aut-name=TokudaMasaaki
en-aut-sei=Tokuda
en-aut-mei=Masaaki
kn-aut-name=徳田雅明
kn-aut-sei=徳田
kn-aut-mei=雅明
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一生理学教室
en-keyword=肝ミトコンドリア
kn-keyword=肝ミトコンドリア
en-keyword=Ca(2+)結合蛋白質
kn-keyword=Ca(2+)結合蛋白質
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=369
end-page=385
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Changes in local blood flow of the brain-stem in acute intracranial hypertension
kn-title=急性頭蓋内圧亢進時における脳幹部の血流変化に関する基礎的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study was carried out to evaluate the effect of supratentorial mass lesions on the local cerebral blood flow (1-CBF) of the brain-stem with special reference to the following three points: the level of the intracranial pressure (ICP) at which the 1-CBF of the brain-stem begins to decrease, alterations in the 1-CBF of the upper brain-stem at the beginning of uncal herniation, and alterations in the 1-CBF of the medulla oblongata during the Cushing's vasopressor response. Platinum electrodes were placed stereotaxically in the thalamus (Th), inferior colliculus (IC) and medulla oblongata (MO) of 40 cats for measurement of 1-CBF by the hydrogen clearance method. The 1-CBF of the brain-stem was serially measured before and after intermittent increases in ICP produced by the inflation of an extradural balloon. Arterial blood pressure, supra- and infratentorial extradural pressure were continuously recorded. The mean control 1-CBF in the Th, IC and MO were 37.5±9.9, 42.1±8.6 and 30.7±4.9 ml/100g/min, respectively. At 20 to 30 mmHg of supratentorial pressure (STP), the 1-CBF of the Th started to decrease, and at 20 to 30 mmHg of infratentorial pressure (ITP), the 1-CBF of the IC started to decrease. Finally at 40 to 60 mmHg of ITP, the 1-CBF of the MO was affected. At the beginning of uncal herniation, indicated by anisocoria, the mean 1-CBF of the IC abruptly decreased from 33.7 to 19.6 ml/100g/min (n=16). In this stage, the pressure gradient between the supra- and infratentorial spaces was 24.6±11.4 mmHg. The Cushing's vasopressor response was evoked at the STP of 93.4±14.6 mmHg and ITP of 49.9±6.8 mmHg (n=16). The blood pressure significantly increased from 121.5 to 140.0 mmHg, however, immediately before and during vasopressor response, there was only a slight, 0.6 mmHg change in the mean cerebral perfusion pressure (CPP) of the posterior fossa. The 1-CBF of the MO also did not show a significant change. The data suggest that in supratentorial mass lesions, the 1-CBF of the thalamus decreases first, followed by the 1-CBF of the inferior colliculus, and then the medulla oblongata, in that order. At the beginning of uncal herniation, the 1-CBF of the upper brain-stem markedly decreased. During the Cushing's vasopressor response, the 1-CBF of the MO did not change significantly.
en-copyright=
kn-copyright=

en-aut-name=SunamiNorio
en-aut-sei=Sunami
en-aut-mei=Norio
kn-aut-name=角南典生
kn-aut-sei=角南
kn-aut-mei=典生
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部脳神経外科学教室
en-keyword=Cerebral blood flow of the brain-stem
kn-keyword=Cerebral blood flow of the brain-stem
en-keyword=Hydrogen clearance method
kn-keyword=Hydrogen clearance method
en-keyword=Intracranial pressure
kn-keyword=Intracranial pressure
en-keyword=Tentorial herniation
kn-keyword=Tentorial herniation
en-keyword=Cushing response
kn-keyword=Cushing response
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=347
end-page=367
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=CSF monoamine metabolites and cyclic nucleotides in depression
kn-title=うつ病における髄液アミン代謝物質と環状ヌクレオチド-うつ病の生物学的研究-
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Monoamine metabolites (5HIAA, HVA and MHPG) and cyclic nucleotides (c-AMP and c-GMP) were determined in lumbar CSF from 16 healthy controls and 36 patients hospitalized for depression. Determination of monoamine metabolites in the depressed patients was performed before and after treatment with antidepressants. The diagnosis was made according to the DSM-III (1980). The following results were obtained: 1) 5HIAA in patients with major depression was significantly lower than in controls regardless of the severity of clinical symptoms, while it was unchanged in patients with the bipolar affective disorder. It is suggested that major depression may differ biologically from the bipolar disorder. 2) The low CSF-5HIAA values in patients with major depression may not be dependent on the clinical state, but rather on a genetic trait, because they were not changed by clinical improvement or treatment with antidepressants. 3) As the initially low CSF-HVA levels in the depressed patients increased along with clinical improvement of the affective retardation, low CSF-HVA levels seem to be clinical state dependent rather than trait dependent in such patients. 4) CSF-MHPG was unchanged in the untreated depressed patients, while it decreased significantly after treatment with any of antidepressants. 5) After treatment both CSF c-AMP and c-GMP levels in completely recovered patients were significantly higher than those in control and un-improved patients. As an increase in c-AMP was found to be related to clinical improvement of depression, it seems to be state dependent in the depressed patients.
en-copyright=
kn-copyright=

en-aut-name=MiyauchiKazuko
en-aut-sei=Miyauchi
en-aut-mei=Kazuko
kn-aut-name=宮内和瑞子
kn-aut-sei=宮内
kn-aut-mei=和瑞子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部神経精神医学教室
en-keyword=うつ病
kn-keyword=うつ病
en-keyword=髄液
kn-keyword=髄液
en-keyword=DSM-III
kn-keyword=DSM-III
en-keyword=アミン代謝物質
kn-keyword=アミン代謝物質
en-keyword=環状ヌクレオチド
kn-keyword=環状ヌクレオチド
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=335
end-page=345
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the determination of urinary protein part III. A new semiquantitative method for determining minute amount of urinary protein by staining with Coomassie Brilliant Blue R-250 of a urine spot on filter paper.
kn-title=尿中の蛋白質の定量の検討 第3報 沪紙Spot-CBB染色法による尿中の微量蛋白質の定量について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A simple and sensitive method for rapid semiquantitive determination of urinary protein was devised. Five μl of the urine to be measured is dropped on the 2cm(2) filter paper, and dried at room temperature. The paper is immersed in a mixture of ethanol, acetic acid and distillated water (35:10:55) which contains 0.2% Coomassie Brilliant Blue R-250 (CBB), and washed with water until the waste water becomes colorless. The paper is again immersed into a mixture but without CBB, after which the paper is rinsed with water again. The protein concentration in the urine sample is determined by comparing the hue of the urine spot with a standard violet hue chart, as well as by the color intensity of the extract of the spot with 3% hydrogen chloride in ethanol at 595 nm by spectrophotometry. This method was applied to the determination of protein in urine from junior college girls immediatly after arriving at school and after cooking study, from adults in a factory at the time of their regular medical examination, and from high school boys before and after physical exercise. The results were compared with that of the TONEIN-TP method. With the SPOTCBB-DYE method, samples can be kept as colored spots on the filter paper, and the protein in urine can be preserved as a spot on the filter paper and measured at any time.
en-copyright=
kn-copyright=

en-aut-name=TanakaYukiko
en-aut-sei=Tanaka
en-aut-mei=Yukiko
kn-aut-name=田中由紀子
kn-aut-sei=田中
kn-aut-mei=由紀子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=Coomassie Brilliant Blue R-250
kn-keyword=Coomassie Brilliant Blue R-250
en-keyword=urinary protein
kn-keyword=urinary protein
en-keyword=沪紙Spot-CBB染色法
kn-keyword=沪紙Spot-CBB染色法
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=317
end-page=334
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Inhibitory effect of histamine on the potentials recorded from the VPM and RF evoked by stimulation of the tooth pulp
kn-title=ヒスタミン脳内投与による鎮痛作用発現について 第2報 ―無麻酔ウサギ歯髄の電気刺激による視床及び中脳網様体誘発電位に及ぼすヒスタミンの影響―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The effect of histamine (Hi) on the evoked potentials recorded from the nucleus ventralis posteromedialis thalami (VPM) and the midbrain reticular formation (RF) was studied in unanesthetized rabbits. Under pentobarbital anesthesia, the recording electrodes were implanted stereotaxically into the VPM and RF, and stimulating electrodes were inserted into the tooth pulp of both incisors of rabbits. To maintain a high electrical resistance between the stimulating electrodes, attention was paid to selecting a suitable material for protecting the soldering point. When the tooth pulp was stimulated by the repetition of square wave pulses either ipsilaterally or contralaterally, similar evoked potentials were recorded at the VPM and RF. The potentials were biphasic consisting of an initial negative deflection followed by a positive deflection. Contralateral stimulation induced larger amplitude waves with shorter latencies than those of ispilateral stimulation. Intraventricular administration of Hi decreased the amplitudes of evoked potentials in a dose-dependent fashion. The effect of 200 μg of Hi corresponds to that produced by intravenous administration of 2 mg/kg of morphine. When the same dose of Hi was given together with 20 μg of pyrilamine, the depression of the potentials was eliminated remarkably, but visible alteration was not brought about by simultaneous administration of cimetidine.
en-copyright=
kn-copyright=

en-aut-name=OkadaIkuo
en-aut-sei=Okada
en-aut-mei=Ikuo
kn-aut-name=岡田郁男
kn-aut-sei=岡田
kn-aut-mei=郁男
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学歯学部口腔外科学第1講座
en-keyword=ヒスタミン
kn-keyword=ヒスタミン
en-keyword=歯髄刺激
kn-keyword=歯髄刺激
en-keyword=誘発電位
kn-keyword=誘発電位
en-keyword=モルヒネ
kn-keyword=モルヒネ
en-keyword=抗ヒスタミン剤
kn-keyword=抗ヒスタミン剤
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=305
end-page=315
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Assessment of mediastinal involvement of esophageal carcinoma using computer tomography (CT)
kn-title=食道癌におけるCT診断 ―他臓器浸潤の検討―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=To assess the extent of mediastinal spread, especially to the left main bronchus and the descending aorta, CT findings were analyzed in 42 patients with mid-thoracic (Im) esophageal carcinoma. The minimum distance between the anterior margin of the vertebral body and the left main bronchus, the minimum inner diameter of the left main bronchus and the maximum size of the tumor were measured and used as diagnostic criteria. CT was capable of evaluating the invasion to the left main bronchus when the minimum distance between the anterior margin of the vertebral body and the left main bronchus was more than 21 mm and the minimum inner diameter of the left main bronchus was less than 8 mm, and to the descending aorta when the tumor was more than 31 mm. CT accurately outlines the extent of lesions and is helpful in determining the type of surgical procedure and in planning radiation therapy.
en-copyright=
kn-copyright=

en-aut-name=OkawaMotoomi
en-aut-sei=Okawa
en-aut-mei=Motoomi
kn-aut-name=大川元臣
kn-aut-sei=大川
kn-aut-mei=元臣
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部放射線医学教室
en-keyword=CT
kn-keyword=CT
en-keyword=食道癌
kn-keyword=食道癌
en-keyword=他臓器浸潤
kn-keyword=他臓器浸潤
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=295
end-page=303
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Model studies on the alteration of the phospholipid composition of Staphyloccous aureus in response to the lack of the cell wall
kn-title=黄色ブドウ球菌の壁欠落にともなう膜リン脂質組成変動のモデル解析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to elucidate why cardiolipin increases markedly in Staphylococcus aureus cells which lack cell walls, the phase transition temperature of cardiolipin (CL) was determined and compared with that of a major phospholipid, phosphatidylglycerol (PG). CL composed of a fatty acid with a given length was synthesized from dimyristoyl PG and dipalmitoyl PG with the aid of phospholipase D prepared from cabbages and was purified by chromatography. Analysis by differential scanning calorimetry showed that the phase transition temperatures of dimyristoyl PG, tetramyristoyl CL, dipalmitoyl PG and tetrapalmitoyl CL were 25.0, 47.0, 40.5 and 62.2℃, respectively. A mixture of the two phospholipids showed a higher phase transition temperature than PG alone, but lower than CL alone. In the presence of divalent cations, especially Ca(2+), the phase transition temperature of CL increased more than that of PG. These results clearly indicate that cardiolipin can increase the membrane rigidity and suggest that S. aureus may increase cardiolipin content of the membrane to compensate for the loss of mechanical protection due to the lack of the cell wall.
en-copyright=
kn-copyright=

en-aut-name=KariyamaReiko
en-aut-sei=Kariyama
en-aut-mei=Reiko
kn-aut-name=狩山玲子
kn-aut-sei=狩山
kn-aut-mei=玲子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部細菌学教室
en-keyword=Differential scanning calorimetry
kn-keyword=Differential scanning calorimetry
en-keyword=Phase transition
kn-keyword=Phase transition
en-keyword=Staphylococcus aureus
kn-keyword=Staphylococcus aureus
en-keyword=Phospholipid composition
kn-keyword=Phospholipid composition
en-keyword=Cardiolipin
kn-keyword=Cardiolipin
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=283
end-page=294
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Immunopathological studies on lupus nephritis; Detection of antibodies to nuclear ribonucleoprotein(RNP) and Sm in kidney eluates.
kn-title=ループス腎炎の免疫病理学的研究腎誘出液における抗RNP,抗Sm抗体の検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=For Immunological and immunopathological studies on lupus nephritis, kidneys from nine autopsied and one nephrectomized patients with systemic lupus erythematosus (SLE) were subjected to immunofluorescence(IF) and elution study. Six patients had diffuse proliferative lupus nephritis, showing granular or lumpy glomerular deposits by IF. Three had chronic renal failure with scanty glomerular deposits. One had no clinical renal disease but showed glomerular mesangial deposits. The serum level of antibodies to native(n)-DNA were slightly or moderately elevated in six patients. Hemolytic activity of serum complement(CH50) was markedly decreased in seven. Elution was performed as follows: the renal cortex was homogenized and washed repeatedly with cold saline, then the sediment containing glomeruli was disrupted by ultrasonication, and then suspended in 0.02 M glycine buffer, pH 2.8, in phosphate buffered saline for the control study. Antibodies to RNP were detected in six instances, and those to Sm in five. The hemagglutinating titers of antibodies to RNP and Sm in acid eluates were 8 to 128 times and 32 to 256 times higher than those in controls, respectively. However, when compared with the hemagglutinating titers in sera, specific enrichment of antibodies to RNP and Sm in acid eluates was less than 8 times. Three of ten acid eluates were demonstrated to contain antibodies to n-DNA which were determined by counterimmunoelectrophoresis(CIE). Two of these showed a positive precipitin reaction against denatured DNA. Minimum amounts of IgG producing positive reaction by CIE were measured in kidney eluates and sera obtained from the same patients. The ratio of the minimum concentrations of IgG in sera to those in eluates ranged between 33 and 125 with n-DNA system. Similarly, the ratio was between 33 and 62 with denatured DNA system. The results suggest that not just DNA immune complex but RNP and Sm immune complexes could be involved in the pathogenesis of lupus nephritis.
en-copyright=
kn-copyright=

en-aut-name=HaraIkuo
en-aut-sei=Hara
en-aut-mei=Ikuo
kn-aut-name=原郁夫
kn-aut-sei=原
kn-aut-mei=郁夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科教室
en-keyword=ループス腎炎
kn-keyword=ループス腎炎
en-keyword=腎誘出液
kn-keyword=腎誘出液
en-keyword=抗Sm抗体
kn-keyword=抗Sm抗体
en-keyword=抗RNP抗体
kn-keyword=抗RNP抗体
en-keyword=抗DNA抗体
kn-keyword=抗DNA抗体
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=271
end-page=282
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Brain active oxygen, free radicals, lipid peroxide and the redox state of glutathione in the Fe(3+) induced epileptic focus of the rat
kn-title=鉄イオンてんかん源性焦点組織のフリーラジカル反応と発作発現機構に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Epileptic discharges were induced 15 minutes after 0.1M FeCl(3) was injected into the rat sensory motor cortex and continued for 6 months after the injection. In order to study the Fe(3+) induced seizure mechanism, Fe(3+) bound methemoglobin, free radicals, active oxygen, malondialdehyde and reduced and oxidized glutathione were measured in the foci of rats after the FeCl(3) injection. It was found that Fe(3+) induced a significant increase in Fe(3+) bound methemoglobin, active oxygen and malondialdehyde and accelerated the glutathione redox reaction 5 minutes after the injection. Fe(3+) was thought to induce lipid peroxidation by free radical reaction and thus produce the peileptic focus.
en-copyright=
kn-copyright=

en-aut-name=KhochiHiroyoshi
en-aut-sei=Khochi
en-aut-mei=Hiroyoshi
kn-aut-name=高知宏喜
kn-aut-sei=高知
kn-aut-mei=宏喜
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部脳代謝研究施設機能生化学部門
en-keyword=鉄イオンてんかん
kn-keyword=鉄イオンてんかん
en-keyword=フリーラジカル
kn-keyword=フリーラジカル
en-keyword=活性酸素
kn-keyword=活性酸素
en-keyword=過酸化脂質
kn-keyword=過酸化脂質
en-keyword=グルタチオン
kn-keyword=グルタチオン
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=263
end-page=270
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on peripheral monocytes in diabetes mellitus. Part II. Lysosomal enzyme activity of monocyte in diabetes mellitus.
kn-title=糖尿病に於ける末梢血単球に関する研究 第2編 単球ライソゾーム酵素活性について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Lysosomal enzyme activities of diabetic monocytes were studied using cytochemical staining of beta-galactosidase and non-specific esterase. The enzyme activity of each monocyte was graded from (-) to (++++) depending on the staining strength. The results were as follows: 1) The beta-galactosidase(β-GAL) activity of monocytes from 23 normal controls was 40.3±20.2% and that of 49 diabetics was 42.6±20.1% , with no difference between diabetics and normal controls in graded β-GAL activity. 2) The degree of diabetic control and the way of therapy did not affect the β-GAL activities. 3) Non-specific esterase(NSE) activities of 37 normal controls and 41 diabetics were 94.0±6.3% and 95.9±6.9% , respectively. The enzyme activities of each grade were different between the two groups, that is, (+++) NSE activity was significantly lower in diabetics than in normal controls (28.7±14.1% vs 47.4±17.9% , P<0.001). On the contrary, (+) and (++) NSE activities of diabetics were significantly higher than those of normal controls. 4) There was no difference in NSE activities depending on the way of therapy. Poorly controlled diabetics had significantly lower (+++) and (++++) NSE activities than others. 5) Further evaluation of NSE activity using the scoring method by Kaplow revealed no difference between diabetics and normal controls. 6) The total monocyte NSE activity, which was calculated from the NSE score multiplied by the absolute monocyte count was lower in diabetics than in normal controls, although there was no difference in the absolute peripheral monocyte count between the two groups.
en-copyright=
kn-copyright=

en-aut-name=MiyaharaKiyoshi
en-aut-sei=Miyahara
en-aut-mei=Kiyoshi
kn-aut-name=宮原潔
kn-aut-sei=宮原
kn-aut-mei=潔
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第2内科教室
en-keyword=糖尿病 (diabetes mellitus)
kn-keyword=糖尿病 (diabetes mellitus)
en-keyword=単球 (monocyte)
kn-keyword=単球 (monocyte)
en-keyword=ライソゾーム酵素 (lysosomal enzyme)
kn-keyword=ライソゾーム酵素 (lysosomal enzyme)
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=253
end-page=262
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on peripheral monocytes in diabetes mellitus. Part I. Monocyte chemotaxis in diabetes mellitus.
kn-title=糖尿病に於ける末梢血単球に関する研究 第1編 単球走性について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Chemotaxis of diabetic monocytes was studied. The use of 0.25 x 10(6) monocytes, 20% Zymosan activated serum and incubation at 37℃ for 90 minutes were found to be the optimal assay conditions for the Boyden chamber method using a nuclepore filter. Under these conditions, monocyte chemotaxis was tested in 48 diabetics and 27 normal controls. The results were as follows: 1) Diabetics had low monocyte chemotaxis, especially in aged patients. The normal controls showed no difference with aging. 2) Poorly controlled diabetics showed significantly lower chemotaxis than both normal controls(P<0.005) and good controlled diabetics(P<0.05). The mean values were 40.1±32.4, 71.3±29.9 and 61.4±36.2 monocytes/HPF, respectively. 3) There was no difference in monocyte chemotaxis between diabetic groups treated with diet alone, oral agents or insulin. 4) Six of nine poorly controlled diabetics showed increased monocyte chemotaxis after being good controlled with insulin.
en-copyright=
kn-copyright=

en-aut-name=MiyaharaKiyoshi
en-aut-sei=Miyahara
en-aut-mei=Kiyoshi
kn-aut-name=宮原潔
kn-aut-sei=宮原
kn-aut-mei=潔
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第2内科教室
en-keyword=糖尿病 (diabetes mellitus)
kn-keyword=糖尿病 (diabetes mellitus)
en-keyword=単球 (monocyte)
kn-keyword=単球 (monocyte)
en-keyword=走性 (chemotaxis)
kn-keyword=走性 (chemotaxis)
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=243
end-page=251
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Function of peripheral blood lymphocytes in patients with systemic lupus erythematosus. Part 2. Significance of null cells in B cell activation
kn-title=全身性エリテマトーデスの末梢血リンパ球機能 第2編 B cell activationにおけるnull cell分画の意義
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Activation of B lineage cells in systemic lupus erythematosus(SLE) has been indicated by the synthesis of IgG without mitogenic stimulation. In the course of this study, a cell population in SLE peripheral blood which synthesized DNA spontaneously and which fractionated into a null cell population was noticed. It was found that cells responsible for hyperproduction of IgG without any stimulation in vitro belonged to the null cell population, not to the sIg(+) cell population. The data suggested that both spontaneous IgG production and DNA synthesis might be performed by the same cell population in SLE. To test whether or not secreted IgG was synthesized by preactivated SLE peripheral blood lymphocytes, these cells were stained with FITC-labeled anti-human Ig antibodies. Significantly more cytoplasmic Ig(+) staining in SLE cells than in normal cells was found. These observations suggest that there was a small group of cells of B cell lineage in the SLE peripheral blood which was stimulated to proliferate and differentiate into a sIg(-) plasmablast line able to secrete IgG.
en-copyright=
kn-copyright=

en-aut-name=MorimasaKimiaki
en-aut-sei=Morimasa
en-aut-mei=Kimiaki
kn-aut-name=盛政公明
kn-aut-sei=盛政
kn-aut-mei=公明
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科学教室
en-keyword=systemic lupus erythematosus
kn-keyword=systemic lupus erythematosus
en-keyword=spontaneous Ig synthesis
kn-keyword=spontaneous Ig synthesis
en-keyword=B cell activation
kn-keyword=B cell activation
en-keyword=null cell fraction
kn-keyword=null cell fraction
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=233
end-page=242
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Function of peripheral blood lymphocytes in patients with systemic lupus erythematosus. Part 1. Characteristics of spontaneously proliferating cells
kn-title=全身性エリテマトーデスの末梢血リンパ球機能 第1編 Spontaneously proliferating cellsの性状
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Increased 3H-Thymidine(3H-TdR) uptake was noticed when peripheral blood mononuclear cells (MNC) from systemic lupus erythematosus(SLE) patients were cultured without any mitogenic stimulation. The peak of 3H-TdR uptake was found immediately after transfering the cells to in vitro culture. DNA synthesizing cells were observed directly by means of autoradiography by pulsing with 3H-TdR for 4 hours. Therefore, it was presumed that there were some MNC in the peripheral blood from SLE patients which were already triggered to proliferate in vivo. These spontaneously proliferating MNC were found to fractionate into a nonadherent, non-E-rosette forming and surface immunoglobulin negative cell population, the so called null cell population. These results suggest that there were activated MNC in the peripheral blood from patients with SLE which fractionated into a null cell population.
en-copyright=
kn-copyright=

en-aut-name=MorimasaKimiaki
en-aut-sei=Morimasa
en-aut-mei=Kimiaki
kn-aut-name=盛政公明
kn-aut-sei=盛政
kn-aut-mei=公明
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科学教室
en-keyword=systemic lupus erythematosus
kn-keyword=systemic lupus erythematosus
en-keyword=peripheral blood lymphocytes
kn-keyword=peripheral blood lymphocytes
en-keyword=spontaneously proliferating cells
kn-keyword=spontaneously proliferating cells
en-keyword=null cell fraction
kn-keyword=null cell fraction
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=225
end-page=232
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Immunological study of lupus nephritis: Demonstration of nuclear ribonucleoprotein(RNP) in diseased kidney from patients with systemic lupus erythematosus.
kn-title=ループス腎炎の免疫学的研究 病変腎組織でのnuclear ribonucleoprotein(RNP)抗原の証明
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Renal tissue from 50 patients with systemic lupus erythematosus (SLE) and 25 with primary glomerulonephritis were tested for the presence of nuclear ribonucleoprotein (RNP) by an immunofluorescent technique. Antibody to RNP was obtained from a patient with mixed connective tissue disease. Fluorescent isothiothianate (FITC) was labelled on purified IgG from the serum. Bright speckled nuclear staining was obtained when the tissue section of mouse kidney was incubated with this conjugate. The staining was completely inhibited when the section was preincubated with non labelled antibody to RNP eluted from RNP immune complex formed in vitro. Granular deposition of RNP antigen in glomeruli was found in 7 of 50 SLE kidneys. The antigen was distributed mainly in the subendothelial site of the glomerular basement membrane and mesangium. The staining was completely inhibited by preincubation of the section with non-labelled antibody to RNP. Deposition of RNP was also found along the tubular basement membrane in 3 of 7 SLE. None of the kidneys from primary glomerulonephritis patients showed positive staining of RNP irrespective of the presence of immunoglobulins and complements in glomeruli. The present observations support the hypothesis that antibody to RNP besides antibody to double stranded DNA might also be of importance in the pathogenesis of renal disease in SLE.
en-copyright=
kn-copyright=

en-aut-name=KurataNoriyuki
en-aut-sei=Kurata
en-aut-mei=Noriyuki
kn-aut-name=倉田典之
kn-aut-sei=倉田
kn-aut-mei=典之
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科教室
en-keyword=全身性エリテマトーデス
kn-keyword=全身性エリテマトーデス
en-keyword=ルーブス腎炎
kn-keyword=ルーブス腎炎
en-keyword=抗核抗体
kn-keyword=抗核抗体
en-keyword=抗RNP抗体
kn-keyword=抗RNP抗体
en-keyword=抗原特異的免疫複合体
kn-keyword=抗原特異的免疫複合体
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=205
end-page=223
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Induction of blastogenesis of peripheral lymphocytes by various mitogens and suppression of blastogenesis by sera in renal allograft recipients.
kn-title=腎移植患者末梢血リンパ球の各種Mitogenによる幼若化反応と,患者血清による幼若反応の抑制
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=It has been reported that a decrease in cellular immunity occurs and a serum immunosuppressive factor exists in uremic patients. In this study, we attempted to examine the change in cellular immunity after renal transplantation, the serum immunosuppressive factor and the usefullness of mitogenic blastogenesis for immunological monitoring. Peripheral blood lymphocytes were isolated by the Ficoll-Conray gradient from 10ml of fresh heparinized blood obtained from renal allograft recipients and healthy individuals. Serum was isolated from 10ml of fresh nonheparinized blood and added in culture after inactivation and sterilization. Pooled AB serum was used for control. Lymphocytes were adjusted to a concentration of 5 x 10(5)/ml in RPMI, supplemented with 60μg/ml Kanamycin and 20% patient's serum or AB serum, and then to 200μl of this suspension, phytohemaggultinin, pokeweed mitogen and concanavalin A were dispensed. The culture was incubated for 72hrs in a humidified atomosphere of 5% C0(2) and air, after which 1μCi tritiated thymidine was added to each well and the incubation continued for 24hrs. The contents of each well were transfered to glassfiber filters, and the amount of isotope incorporated was determined in a liquid scintillation counter. PHA and PWM blastogenesis of patients treated with hemodialysis were significantly decreased. PHA, PWM and Con A blastogenesis of renal allograft patients were significantly decreased, but turned to recover 1 year after renal transplantation. Sera of renal allograft patients suppressed all kinds of blastogenesis of healthy individuals, but only PHA and PWM blastogenesis of renal allograft patients themselves. In renal allograft patients with good renal function 1 year after transplantation, Con A autosuppression was very weak and negative suppression increased significantly. There were correlations between preoperative PHA blastogenesis and acute rejection, and between postoperative PWM blastogenesis and chronic rejection. From these findings, it is concluded that clear elavation of PHA, PWM blastogenesis and suppression of Con A blastogenesis are slightly usefull for immunological monitoring of acute and chronic rejection.
en-copyright=
kn-copyright=

en-aut-name=OritaYojiro
en-aut-sei=Orita
en-aut-mei=Yojiro
kn-aut-name=折田洋二郎
kn-aut-sei=折田
kn-aut-mei=洋二郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科教室
en-keyword=PHA
kn-keyword=PHA
en-keyword=PWM
kn-keyword=PWM
en-keyword=Con A
kn-keyword=Con A
en-keyword=幼若化反応
kn-keyword=幼若化反応
en-keyword=腎移植
kn-keyword=腎移植
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=195
end-page=203
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Extraction of tumor membrane protein and it's use in a skin test for immunological monitoring Part 2. Skin tests using autologous tumor membrane extracts
kn-title=腫瘍膜蛋白の抽出および免疫学的指標としての抽出蛋白による皮内反応 第2編　自家腫瘍抽出膜蛋白による皮内反応と臨床経過
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Autologous skin tests with DOC-solubilized-tumor extracts including 20 cases of colon cancer and 25 cases of gastric cancer revealed the following: 1. There were 9 positive reactions and 11 negative reactions in the colon cancer cases. On the other hand, there were 16 positive reactions and 9 negative reactions in the gastric cancer cases. 2. In gastric cancer cases, the time span of autologous skin tests revealed that curable cases with positive skin reactions had negative reactions after operation and uncurable cases remained negative after operation. 3. There was no relationship of the clinical stage and nonspecific skin test (PPD skin test) to the autologous skin test.
en-copyright=
kn-copyright=

en-aut-name=KuroseYasuhei
en-aut-sei=Kurose
en-aut-mei=Yasuhei
kn-aut-name=黒瀬康平
kn-aut-sei=黒瀬
kn-aut-mei=康平
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科学教室
en-keyword=腫瘍抽出膜蛋白
kn-keyword=腫瘍抽出膜蛋白
en-keyword=皮内テスト
kn-keyword=皮内テスト
en-keyword=腫瘍特異抗原
kn-keyword=腫瘍特異抗原
END

start-ver=1.4
cd-journal=joma
no-vol=95
cd-vols=
no-issue=3-4
article-no=
start-page=185
end-page=193
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Extraction of tumor membrane protein and it's use in a skin test for immunological monitoring Part 1, Comparison of macrophage migration inhibition activity between DOC- and 3MKCL-solubilized- tumor membrane extract
kn-title=腫瘍膜蛋白の抽出および免疫学的指標としての抽出蛋白による皮内反応 第1編 DOCおよび3MKCL抽出膜蛋白によるマクロファージ遊走阻止活性の比較 ―特に抽出方法の違いによるマクロファージ遊走阻止活性の差を中心として―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Ehrlich ascites cancer cells and MH134 cells were extracted with Deoxycholate(DOC) or 3MKCL. The antigenicity of these extracts was studied with the macrophage migration inhibitory test (MI-test), and the results can be presented briefly as follows: 1. The extract from DOC solubilized Ehrlich ascites cancer cells had about 50 times the antigenic titer of the extract from 3MKCL solubilized cells. 2. The extract from DOC solubilized MH134 cells had an antigenic effect at 500ug/ml but the extract from 3MKCL solubilized cells had no effect at any concentration. 3. These extracts were fractionated with Sephadex G-200 and the antigenic sites were determined with the MI-test.
en-copyright=
kn-copyright=

en-aut-name=KuroseYasuhei
en-aut-sei=Kurose
en-aut-mei=Yasuhei
kn-aut-name=黒瀬康平
kn-aut-sei=黒瀬
kn-aut-mei=康平
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科教室
en-keyword=DOC 3 MKCL MIF
kn-keyword=DOC 3 MKCL MIF
en-keyword=エールリッヒ腹水癌細胞
kn-keyword=エールリッヒ腹水癌細胞
en-keyword=MH 134細胞
kn-keyword=MH 134細胞
END