start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=853 end-page=862 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Changes in the subjective symptoms, flicker value and the excretions of urinary 17-OHCS, protein and creatinine after physical and mental stress kn-title=肉体ならびに精神労作負荷前後の自覚症状，フリッカー値及び尿中17-OHコルチコステロイド・蛋白質・クレアチニン排泄量の変動 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ten students who belong to the volleyball club in a women's university practiced vollyball for 2 hours; 35 female students were walked for 1 hour (Mean heart rate: 105 beats/min). 32 and 6 female students took the one digit addition of Kraepelin's test for 60 and 90 min, respectively. The number of complaints of subjective symptoms, flicker fusion value, the excretion rate of urinary 17-OHCS (17-hydroxycorticosteroids), protein and creatinine before and after physical or mental work were investigated. The questionnaire included thirty questions, divided into three groups each consisting of ten symptoms, I: drowsiness and dullness, II: difficulty in concentration, III: projection of physical disintegration. The results obtained were as follows: 1) The number of subjective symptoms in those who walked were particulary increased in groups I and III. Group II symptoms were increased in the participants who calculated the one digit addition. 2) The flicker fusion value after physical exercise was not decresed, but in one digit addition the flicker fusion value was significant decreased after working. 3) The excretion of urinary 17-OHCS tended to decrease after vollyball training and walking. In the case of one digit addition, the excretion of urinary 17-OHCS was increased. 4) The excretion of urinary protein was generally increased after vollyball training, and urinary creatinine was increased after physical and mental work. In conclusion a difference was demonstrated between physical and mental work and related complaints of subjective symptoms, flicker fusion values and excretion rates of urinary 17-OHCS. en-copyright= kn-copyright= en-aut-name=NakaeSeitaro en-aut-sei=Nakae en-aut-mei=Seitaro kn-aut-name=中永征太郎 kn-aut-sei=中永 kn-aut-mei=征太郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生学講座 en-keyword=労作時の疲労感 kn-keyword=労作時の疲労感 en-keyword=フリッカー値 kn-keyword=フリッカー値 en-keyword=尿排泄成分量 kn-keyword=尿排泄成分量 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=843 end-page=852 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Variations in blood cholinesterase activity level in termite control workers using chlorpyrifos kn-title=クロルピリホス使用シロアリ防除作業者の血中コリンエステラーゼ活性値の変動について en-subtitle= kn-subtitle= en-abstract=In order to clarify the effect of chlorpyrifos on workers using chlorpyrifos for termite control, changes in the cholinesterase activity in the blood of 6 workers was surveyed. Cholinesterase activity in the plasma and erythrocytes decreased in the busy season from May to September and recovered in the off season of January. The ratio of the lowest level of plasma cholinesterase activity from a worker in the busy season to the average activities of a worker examined several times in the off season, was examined in each workers. The ratio was under 50% in 6 workers using chlorpyrifos. The ratio of the mean cholinesterase activity in the erythrocytes of a worker in the busy season to that in the off season was examined, and was about 70% in 6 workers. A negative correlation between working time and increasing ratio of plasma cholinesterase was significant. A marked change in the subjective and objective symptoms of workers was not absent in busy season. Although some workers showed a decrease in the number of erythrocytes and leukocytes, and an increase in serum lipase activities, and abnormalities in the serum lipids. The data indicated that plasma cholinesterase activity levels are useful for biological exposure monitoring of chlorpyrifos. kn-abstract=1986年9月よりシロアリ防除剤として広く使用されだしたクロルピリホス(有機リン系殺虫剤)のシロアリ防除作業者への健康影響を明らかにするために,シロアリ防除事業所の従業員8名について追跡調査を行い,以下の結果を得た. 1.処理作業従事者(6名)の毎月初めの平均血漿コリンエステラーゼ活性値は1987年4月までは正常範囲であったが,シロアリ防除シーズン(繁忙期)の5月より低下を始め, 6月〜9月は正常値の下限(0.6ΔpH)以下であった.その後閑散期には回復に向かい, 1988年1月にはシーズン前の水準に戻った.各作業者においては,シーズン中の最低値は,シーズン前の水準(1986年11月〜1987年4月の各人の平均値)の50%以下であり,うち2名は10%以下にまで低下した.一方,主として営業従事者の2名の血漿コリンエステラーゼ活性値はシーズン中にやや低下したものの正常値の範囲内であり,シーズン中の最低値もシーズン前の水準の60%以上であった. 2.赤血球コリンエステラーゼ活性値は, 1987年6月から1988年1月まで8名とも正常範囲であったが, 6〜9月は低く, 12月及び翌年の1月は高値であった.前者の期間における各人の平均値の後者の期間における各人の平均値に対する割合は, 8人の平均で72.2%であった. 3. 1作業者の観察において,シーズン中の処理作業従事時間数と血漿コリンエステラーゼ活性値の増加率との間には有意の負の相関が認められ, 1日の時間数が2時間で血漿コリンエステラーゼ活性値は横這い,それ以上で低下,それ以下で上昇することが明らかになった. 4.作業者の自覚症状,他覚症状には顕著な異常は認められていない.作業者によっでは赤血球数および白血球数の若干の低下,血清脂質およびリパーゼの異常等が認められており継続観察の必要がある. 5.血漿コリンエステラーゼ活性値はクロルピリホスの生物学的暴露指標として鋭敏であり,クロルピリホスによる健康障害の予防のために有用である. en-copyright= kn-copyright= en-aut-name=JitsunariFumihiko en-aut-sei=Jitsunari en-aut-mei=Fumihiko kn-aut-name=實成文彦 kn-aut-sei=實成 kn-aut-mei=文彦 aut-affil-num=1 ORCID= en-aut-name=AsakawaFumiyuki en-aut-sei=Asakawa en-aut-mei=Fumiyuki kn-aut-name=浅川冨美雪 kn-aut-sei=浅川 kn-aut-mei=冨美雪 aut-affil-num=2 ORCID= en-aut-name=NakajimaTaichi en-aut-sei=Nakajima en-aut-mei=Taichi kn-aut-name=中嶋泰知 kn-aut-sei=中嶋 kn-aut-mei=泰知 aut-affil-num=3 ORCID= en-aut-name=ShimadaJunko en-aut-sei=Shimada en-aut-mei=Junko kn-aut-name=島田潤子 kn-aut-sei=島田 kn-aut-mei=潤子 aut-affil-num=4 ORCID= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name=緒方正名 kn-aut-sei=緒方 kn-aut-mei=正名 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=香川医科大学人間環境医学講座 affil-num=2 en-affil= kn-affil=香川医科大学人間環境医学講座 affil-num=3 en-affil= kn-affil=香川医科大学人間環境医学講座 affil-num=4 en-affil= kn-affil=前田病院 affil-num=5 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 en-keyword=クロルピリホス kn-keyword=クロルピリホス en-keyword=シロアリ防除作業者 kn-keyword=シロアリ防除作業者 en-keyword=有機リン中毒 kn-keyword=有機リン中毒 en-keyword=コリンエステラーゼ活性値 kn-keyword=コリンエステラーゼ活性値 en-keyword=生物学的暴露指標 kn-keyword=生物学的暴露指標 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=831 end-page=841 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Medical service situation by city and town: A classification attempt using principal component analysis - On out-patient medical treatment in 43 cities and towns of Kagawa Prefecture - kn-title=市町別医療需給状況と主成分分析法を用いたその類型化の試み―香川県下の43市町の外来医療について― en-subtitle= kn-subtitle= en-abstract= kn-abstract=The medical service situation of out-patient medical treatment in 43 cities and towns of Kagawa Prefecture was analyzed, and classified using principal component analysis. When viewed by the average of 43 cities and towns, the rate of dependence on one's own area was 48.4%, the rate of patients from one's own area at 73.3%. Generally, the self-sufficiency of medicalcare was high, and the medical treatment zone was wider in the city region compared with the county region. The medical service situation in 43 cities and towns was classified into 5 types such as the Nuclear type: 4 cities; the Base type: 1 city and 4 towns; the Averageother type: 18 towns; the Peripheral type (the Insular region type: 3 towns; the Peninsular or remote place type: 2 towns; the Satellite city type: 2 towns) and the Depopulated type: 9 towns, using principal component analysis with the rate of dependence on one's own area, rate of patients from one's own area, dependence entropy and medical treatment zone entropy as the input data. en-copyright= kn-copyright= en-aut-name=JitsunariFumihiko en-aut-sei=Jitsunari en-aut-mei=Fumihiko kn-aut-name=實成文彦 kn-aut-sei=實成 kn-aut-mei=文彦 aut-affil-num=1 ORCID= en-aut-name=AsakawaFumiyuki en-aut-sei=Asakawa en-aut-mei=Fumiyuki kn-aut-name=浅川冨美雪 kn-aut-sei=浅川 kn-aut-mei=冨美雪 aut-affil-num=2 ORCID= en-aut-name=NakajimaTaichi en-aut-sei=Nakajima en-aut-mei=Taichi kn-aut-name=中嶋泰知 kn-aut-sei=中嶋 kn-aut-mei=泰知 aut-affil-num=3 ORCID= en-aut-name=ManabeYoshiki en-aut-sei=Manabe en-aut-mei=Yoshiki kn-aut-name=真鍋芳樹 kn-aut-sei=真鍋 kn-aut-mei=芳樹 aut-affil-num=4 ORCID= en-aut-name=GotoAtsushi en-aut-sei=Goto en-aut-mei=Atsushi kn-aut-name=後藤敦 kn-aut-sei=後藤 kn-aut-mei=敦 aut-affil-num=5 ORCID= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name=緒方正名 kn-aut-sei=緒方 kn-aut-mei=正名 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=香川医科大学人間環境医学講座 affil-num=2 en-affil= kn-affil=香川医科大学人間環境医学講座 affil-num=3 en-affil= kn-affil=香川医科大学人間環境医学講座 affil-num=4 en-affil= kn-affil=香川医科大学人間環境医学講座 affil-num=5 en-affil= kn-affil=香川医科大学人間環境医学講座 affil-num=6 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 en-keyword=医療需給 kn-keyword=医療需給 en-keyword=医療施設利用圏 kn-keyword=医療施設利用圏 en-keyword=診療圏 kn-keyword=診療圏 en-keyword=主成分分析法 kn-keyword=主成分分析法 en-keyword=医療依存関係 kn-keyword=医療依存関係 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=821 end-page=830 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Parasitic diseases brought to the Department of Parasitology, in five years, 1984-1989 kn-title=最近5年間に経験した寄生虫・衛生動物疾患症例について(1984-1989) en-subtitle= kn-subtitle= en-abstract= kn-abstract=From April 1984 to March 1989, we accepted 399 samples and specimens from hospitals and clinics, and diagnosed 86 cases of parasitic diseases. In this paper, 14 case-reports of zoonoses, larva migrans, imported disease and opportunistic infection are presented and discussed. en-copyright= kn-copyright= en-aut-name=MatsuokaHiroyuki en-aut-sei=Matsuoka en-aut-mei=Hiroyuki kn-aut-name=松岡裕之 kn-aut-sei=松岡 kn-aut-mei=裕之 aut-affil-num=1 ORCID= en-aut-name=AjiToshiki en-aut-sei=Aji en-aut-mei=Toshiki kn-aut-name=安治敏樹 kn-aut-sei=安治 kn-aut-mei=敏樹 aut-affil-num=2 ORCID= en-aut-name=IshiiAkira en-aut-sei=Ishii en-aut-mei=Akira kn-aut-name=石井明 kn-aut-sei=石井 kn-aut-mei=明 aut-affil-num=3 ORCID= en-aut-name=TonguYasumasa en-aut-sei=Tongu en-aut-mei=Yasumasa kn-aut-name=頓宮廉正 kn-aut-sei=頓宮 kn-aut-mei=廉正 aut-affil-num=4 ORCID= en-aut-name=OhtaNobuo en-aut-sei=Ohta en-aut-mei=Nobuo kn-aut-name=太田伸生 kn-aut-sei=太田 kn-aut-mei=伸生 aut-affil-num=5 ORCID= en-aut-name=EdahiroToru en-aut-sei=Edahiro en-aut-mei=Toru kn-aut-name=枝広徹 kn-aut-sei=枝広 kn-aut-mei=徹 aut-affil-num=6 ORCID= en-aut-name=DoiHiroyuki en-aut-sei=Doi en-aut-mei=Hiroyuki kn-aut-name=土居弘幸 kn-aut-sei=土居 kn-aut-mei=弘幸 aut-affil-num=7 ORCID= en-aut-name=KimuraJulieta Yuri en-aut-sei=Kimura en-aut-mei=Julieta Yuri kn-aut-name=木村ジュリエッタ・Ｙ kn-aut-sei=木村 kn-aut-mei=ジュリエッタ・Ｙ aut-affil-num=8 ORCID= en-aut-name=ItanoKazuo en-aut-sei=Itano en-aut-mei=Kazuo kn-aut-name=板野一男 kn-aut-sei=板野 kn-aut-mei=一男 aut-affil-num=9 ORCID= en-aut-name=MorishigeKazuhisa en-aut-sei=Morishige en-aut-mei=Kazuhisa kn-aut-name=森重和久 kn-aut-sei=森重 kn-aut-mei=和久 aut-affil-num=10 ORCID= en-aut-name=SongJigo en-aut-sei=Song en-aut-mei=Jigo kn-aut-name=宋晶 kn-aut-sei=宋 kn-aut-mei=晶 aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=8 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=9 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=10 en-affil= kn-affil=岡山大学医学部寄生虫学教室 affil-num=11 en-affil= kn-affil=岡山大学医学部寄生虫学教室 en-keyword=人畜共通感染症 kn-keyword=人畜共通感染症 en-keyword=輸入寄生虫病 kn-keyword=輸入寄生虫病 en-keyword=日和見感染症 kn-keyword=日和見感染症 en-keyword=幼虫移行症 kn-keyword=幼虫移行症 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=811 end-page=820 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Membrane lipids of Yersinia enterocolitica and influence of culture temperature on membrane lipid composition kn-title=Yersinia enterocoliticaの膜脂質解析および培養温度変換による適応変化 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The author studied the membrane lipid and fatty acid compositions of Yersinia enterocolitica. Furthermore, the author studied the adaptation of the membrane to changes in culture temperatures, and characterized the physical state of membrane lipid by nylon microcapsule method developed by Okahata and Nagamachi. Phosphatidylglycerol, phosphatidylethanolamine and cardiolipin were the main phospholipids of the membrane. The ratio of these phospholipid compositions was not changed by culture temperature. Fatty acids were found to be C(14:0), C(15:0), C(16:0), C(16:1), C(17:0), C(18:0), C(18:1) and cyclopropane C(17:0). The last was identified by gaschromatography-massspectrometry and hydrogenation. In 37°C cultures, saturated fatty acids were increased and unsaturated fatty acids were decreased in comparison with those from 25°C cultures. Cyclopropane C(17:0) was also increased in 37°C cultures. These changes appear to be necessary for maintaining membrane stability at high temperatures. Using NaCI-release from 0.2M NaCI-entrapped nylon microcapsules coated with the membrane lipids as an indicator, it was found that phase transition of lipid membranes from 25°C cultures and 37°C cultures occurred approximately at 30°C and 45°C, respectively. The differences in the phase transition temperature seem to correlate with the adaptive changes in fatty acid composition by culture temperatures. en-copyright= kn-copyright= en-aut-name=ShibuyaSei-ichiro en-aut-sei=Shibuya en-aut-mei=Sei-ichiro kn-aut-name=澁谷誠一郎 kn-aut-sei=澁谷 kn-aut-mei=誠一郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部細菌学教室 en-keyword=Yersinia enterocolitica kn-keyword=Yersinia enterocolitica en-keyword=細胞膜 kn-keyword=細胞膜 en-keyword=リン脂質 kn-keyword=リン脂質 en-keyword=脂肪酸 kn-keyword=脂肪酸 en-keyword=2分子膜 kn-keyword=2分子膜 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=797 end-page=810 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of bronchial asthma Part 2. Studies of the changes in the peripheral airway of asthmatic patients by selective alveolo-bronchography (SAB) kn-title=気管支喘息に関する研究 第2編 選択的肺胞気管支造影法による気管支喘息の末梢気道病変の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to investigate changes in the peripheral airway of asthmatics, selective aveolobronchography (SAB) was performed in 33 cases of asthma. SAB findings were studied in respect to the classification of asthma and also examined with results of pulmonary function test, bronchoalveolar lavage (BAL) and transbronchial lung biopsy. 1. Narrowing of the central airway was observed in almost all cases and 19 cases showed narrowing of the peripheral airways. An uneven appearance of the alveolar figure was observed in 11 cases. 2. Narrowing of the peripheral airway and an uneven appearance of the alveolar figure were often observed in non-atopic patients, in moderate or severe cases and in late-onset asthmatics. 3. The mean value of V(50)/V(25) was increased and the mean value of %V(25) was decreased in patients with narrowing of the peripheral airways and also in patients with uneven appearance of the alveolar figure. 4. An increase of neutrophils was noticed in the BAL fluid of patients with narrowing of the peripheral airways. 5. With histological study by TBLB, infiltration of eosinophils into the alveoli as well as mononuclear cells into alveoli and bronchioli was demonstrated in patients with narrowing of the peripheral airways. en-copyright= kn-copyright= en-aut-name=NakamuraYukinobu en-aut-sei=Nakamura en-aut-mei=Yukinobu kn-aut-name=中村之信 kn-aut-sei=中村 kn-aut-mei=之信 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=選択的肺胞気管支造影 kn-keyword=選択的肺胞気管支造影 en-keyword=気管支喘息 kn-keyword=気管支喘息 en-keyword=末梢気道病変 kn-keyword=末梢気道病変 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=791 end-page=796 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of bronchial asthma Part 1. Clinical characteristics of late onset asthma kn-title=気管支喘息に関する研究 第1編 中高年発症型気管支喘息の臨床的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to clarify the immunological characteristics of late onset asthma, 106 cases of asthma in patients over 40 years old were divided into two groups: the early onset asthma group at an age less than 40 years old and the late onset asthma group which was 40 or more years old at onset. The results obtained in each group were compared. 1) Asthma patients with a positive family history or with other allergic diseases in the past history were significantly less in the late onset asthma group compared with the early onset asthma group. 2) An increased eosinophil count in the peripheral blood was observed in both groups with no significant difference. 3) There were more patients with low IgE level (less than 300IU/ml) in the late onset asthma group than in the early onset asthma group. 4) In skin tests to 8 common allergens, the incidence of positive immediate reaction to house dust and aspergillus was low in the late onset asthma group. There was no difference in late or delayed reactions between the two groups. 5) The number of patients with positive serum specific IgE antibody to house dust or mites were few in the late onset asthme group. 6) In the bronchial provocation test with house dust, the proportion of positive response was low in the late onset asthme group. 7) The attack symptoms of asthma were severe and perennial in the late onset asthma group compared with those of the early onset asthma group. en-copyright= kn-copyright= en-aut-name=NakamuraYukinobu en-aut-sei=Nakamura en-aut-mei=Yukinobu kn-aut-name=中村之信 kn-aut-sei=中村 kn-aut-mei=之信 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=気管支喘息 kn-keyword=気管支喘息 en-keyword=発症年齢 kn-keyword=発症年齢 en-keyword=病型 kn-keyword=病型 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=781 end-page=789 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Clinical studies of relapse in acute leukemia Part 2. Immunosuppressive acidic protein (IAP) in acute leukemia kn-title=急性白血病再燃病態並びに再燃予知に関する研究 第2編 急性白血病における血清IAP値と再燃予知に関する検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Serum levels of immunosuppressive acidic protein (IAP) in 17 patients with acute leukemia were measured. The patients included 5 with acute myelocytic leukemia (AML), 4 with acute promyelocytic leukemia (APL), 3 with acute monocytic/myelomonocytic leukemia (AMoL/AMMoL), and 5 with acute lymphocytic leukemia (ALL). Serum IAP levels in untreated acute leukemia patients were higher than those in normal healthy individuals (763.2±321.1 ng/ml vs 389.8±96.7 ng/ml, p<0.05). They tended to decrease to a normal range in complete remission. A correlation was observed between serum IAP levels and the leukemic cell population in the bone marrow (r=0.39, p<0.05). Serum IAP levels increased in febrile patients compared to those in afebrile patients (p<0.05). Patients with more than 500ng/ml of IAP in remission had more risk of relapse in comparison to those with less than 500ng/ml (7 of 10, 70% vs 2 of 15, 13.3%, p<0.05). These results suggest that the measurement of serum IAP is useful for monitoring the leukemic patient. en-copyright= kn-copyright= en-aut-name=TakizawaMichihiro en-aut-sei=Takizawa en-aut-mei=Michihiro kn-aut-name=滝沢道弘 kn-aut-sei=滝沢 kn-aut-mei=道弘 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=急性白血病 kn-keyword=急性白血病 en-keyword=再燃病態 kn-keyword=再燃病態 en-keyword=再燃予知 kn-keyword=再燃予知 en-keyword=血清IAP値 kn-keyword=血清IAP値 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=771 end-page=780 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Clinical studies of relapse in acute leukemia Part 1. Cellular composition of bone marrow in remission and prediction of relapse in acute leukemiaPart 1. Cellular composition of bone marrow in remission and prediction of relapse in acute leukemia kn-title=急性白血病再燃病態並びに再燃予知に関する研究 第1編 急性骨髄性白血病における寛解時骨髄細胞構成比と再燃予知に関する検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The cellular composition of remission marrow from patients with acute myelocytic leukemia (AML) was studied in order to obtain informations for the prediction of relapse. Hematological findings such as (1) Myeloblast 3%, (2) Promyelocyte 12%, (3) Erythroblasts 45% and (4) Erythroblasts 15% were thought to be atypical in remission marrow. Among these atypical finings, the coexsistence of (1) and (3) or (1) and (4) were important in the prediction of relapse. In the first month after the recognition of (1)+(3) or (1)+(4), the cumulative relapse rate was 35% and 50%, respectively. The rates were 71% and 58% by the second month, respectively. The median remission duration of patients treated within two weeks after the recognition of (1) was longer than that of patients treated after two weeks (4.7 months vs 1.5 months, p<0.05). These results indicate that the coexsistence of atypical findings, (1)+(3) or (1)+(4), are important not only in the predicting of the relapse of AML but also to analyze the dyshemopoiesis of preleukemic states or myelodysplastic syndromes. en-copyright= kn-copyright= en-aut-name=TakizawaMichihiro en-aut-sei=Takizawa en-aut-mei=Michihiro kn-aut-name=滝沢道弘 kn-aut-sei=滝沢 kn-aut-mei=道弘 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=急性白血病 kn-keyword=急性白血病 en-keyword=急性白血病再燃 kn-keyword=急性白血病再燃 en-keyword=再燃予知 kn-keyword=再燃予知 en-keyword=寛解時骨髄 kn-keyword=寛解時骨髄 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=763 end-page=770 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of prostaglandin E(1) and prostaglandin F(2α) on bone formation of cultured chick embryonic bone with special reference to the alkaline phosphatase activity kn-title=プロスタグランディンE(1)およびF(2α)の鶏胚骨の骨形成におよぼす影響―アルカリホスファターゼ活性に対する作用― en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of prostaglandin E(1) (PGE(1)) and prostaglandin F(2α) (PGF(2α)) on bone formation were investigated with femur and tibia from 10-day chick embryo. When the bones were cultured in serum free medium, PGE(1) (10nM) caused a significant increase of bone alkaline phosphatase activity and a slight increase in bone protein content, whereas PGF(2α) (100nM) significantly decreased the enzyme activity. On the other hand, in a medium containing 20% serum, PGE1 did not affect the enzyme activity at 10nM, but slightly increased the activity at 1nM. No effect of PGE(1) on bone calcium content was observed in a concentration ranging from 0.1nM to 100nM. Thus, PGE1 was likely to stimulate bone formation at 10nM, whereas PGF(2α) supressed formation at 100nM. en-copyright= kn-copyright= en-aut-name=UedaShigeki en-aut-sei=Ueda en-aut-mei=Shigeki kn-aut-name=上田茂樹 kn-aut-sei=上田 kn-aut-mei=茂樹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学歯学部口腔外科学第1講座 en-keyword=組織培養 kn-keyword=組織培養 en-keyword=プロスタグランディン kn-keyword=プロスタグランディン en-keyword=骨形成 kn-keyword=骨形成 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=743 end-page=762 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Acute brain swelling and edema by stimulation of the medullary reticular formation in the cold injured brain kn-title=凍結脳損傷における延髄網様体刺激の脳浮腫および頭蓋内圧におよぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The purpose of the present study is to clarify the effects of stimulation of the medullary reticular formation on brain edema and ICP in a cerebral contusion model. The study was divided into two experiments. Experiment 1 (32 cats). The effet of stimulation of the medullary reticular formation on tissue water permeability was assessed using normal brain and cold-induced edema. The cold injury was inflicted by applying a freezing probe of -50°C directly on the dura for 1 minute. The animals were divided into 4 groups. Group 1: intermittent electrical stimulation of the reticular formation of the medulla oblongata (P(10), L±(2.5), H(-9.0)) of a normal brain for 40 minutes; Group 2: cold lesion only; Group 3: same procedure as group 1 on cold-induced edema; Group 4: administration of Angiotensin II for 60 minutes. Water contents of the 4 groups were measured by a gravimetric technique from coronal slices across the lesion. Experiment 2 (24 cats). The ICP and local cerebral blood volume (CBV) and blood pressure (BP) were continuously measured during stimulation of the medullary reticular formation in animals with cold-induced edema. The normal water content of the white matter was 67.7±0.9% g water/g tissue. Seventeen hours following cold-induced edema, the water content adjacent to the lesion increased to 71.8% and gradually decreased at areas further from the lesion. There were no significant changes in water content in the contralateral hemisphere. Medullary stimulation of brains with cold-induced edema produced a widespread significant increase in tissue water content in the injured hemisphere by 1.6 to 3.8%. In particular water contents adjacent to the lesion were significantly higher, by 1.3 to 1.8%, than those of group 4 with induced hypertension. In experiment 2, the BP, ICP and CBV increased abruptly immediately after stimulation and the response patterns were divided into 2 groups. In 15 of 24 animals (62%), temporary elevation of the ICP ranging from 10 to 25mmHg was observed during stimulation with a concomitant increase in CBV and BP. In 9 animals (38%), progressive increase in ICP and CBV were produced by stimulation and even after cessation of stimulation, ICP progressively increased up to 60 to 100mmHg. Progressively increased ICPs were evoked with stimulation of the paramedian reticular formation of the medulla oblongata, that is, the region around the nucleus reticularis parvocellularis. Stimulation of the medullary reticular formation accelerated brain water permeability and increased CBV, resulting in acute intracranial hypertension in an injured brain. en-copyright= kn-copyright= en-aut-name=KawachiMasamitsu en-aut-sei=Kawachi en-aut-mei=Masamitsu kn-aut-name=河内正光 kn-aut-sei=河内 kn-aut-mei=正光 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳神経外科教室 en-keyword=acute brain swelling kn-keyword=acute brain swelling en-keyword=brain edema kn-keyword=brain edema en-keyword=water content kn-keyword=water content en-keyword=cerebral blood volume kn-keyword=cerebral blood volume en-keyword=medulla oblongata kn-keyword=medulla oblongata END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=733 end-page=742 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of iron metabolism of the lung Part 2. An electron microscopic study of ferritin synthesis in alveolar macrophages kn-title=肺における鉄代謝に関する研究 第2編 肺胞マクロファージのフェリチン合成能に関する電顕学的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to clarify the role of alveolar macrophages (alv Mφ) in the iron metabolism of the lung, ultrastructural findings of cells obtained by bronchoalveolar lavage were studied by transmission electron microscopy in rats after inhalation of saccharated iron oxide and welding fumes as well as a patient with welder's lung. Alv Mφ of rats which had inhaled saccharated iron oxide formed phagosomes and secondary lysosomes 4 hours after inhalation. The first day after inhalation, many ferritin particles were seen in cytosol and formed a diffuse patterns; 19% of alv Mφ had ferritin particles in a paracrystalin arrangement in the secondary lysosomes. Electron micrographs of alv Mφ at 3, 6, 14, and 28 days after inhalation showed an increase of the cells with ferritin particles. Alv Mφ of rats which had inhaled welding fumes phagocytized those iron particles and also synthesized ferritin particles in the cytosol and secondary lysosomes 6 days after inhalation. Numerous ferritin particles were seen in the cytosol and secondary lysosomes of alv Mφ from a patient with welder's lung as well. These results suggest that inhaled iron was phagocytized by alv Mφ and stored in the cytosol by binding with apoferritin which was synthesized by the ribosomes. en-copyright= kn-copyright= en-aut-name=FujiwaraKanta en-aut-sei=Fujiwara en-aut-mei=Kanta kn-aut-name=藤原寛太 kn-aut-sei=藤原 kn-aut-mei=寛太 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=フェリチン kn-keyword=フェリチン en-keyword=担鉄肺胞マクロファージ kn-keyword=担鉄肺胞マクロファージ en-keyword=溶接工肺 kn-keyword=溶接工肺 en-keyword=ラット kn-keyword=ラット en-keyword=透過型電顕 kn-keyword=透過型電顕 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=723 end-page=732 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of iron metabolism of the lung Part 1. Influence of inhaled iron on iron metabolism of an experimental welder's lung kn-title=肺における鉄代謝に関する研究 第1編 実験的溶接工肺における吸入鉄の代謝について en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to clarify the mechanism of elevated iron stores in welder's lungs, the cellular contents of bronchoalveolar lavage (BAL) fluid, the serum iron, total iron binding capacity, liver non-hemin iron and histological findings were examined in experiments using Wister rats exposed to inhalation of welding fumes for 4, 8, Total cell counts, the percentage of neutrophils and iron laden alveolar macrophages in the BAL fluid increased after continuous inhalation, but they were decreased 3 months after inhalation. Serum iron, transferrin saturation, and liver non-hemin iron in rats exposed to inhalation for 8 and 12 weeks were significantly elevated (p<0.01). Iron stores of rats 3 months after inhalation were elevated more than levels 1 week after inhalation. Histological findings in the lung showed a slight thickening of alveolar wall with cellular infiltration. Iron was deposited predominantly in alveolar macrophages of the alveolar space and in connective tissue macrophages. Iron deposition was also seen in the red pulp of the spleen after inhalation. These data suggest that iron was highly stored in the whole body of patients with welder's lung, and that alveolar macrophages play an important role in the iron metabolism of the lung. en-copyright= kn-copyright= en-aut-name=FujiwaraKanta en-aut-sei=Fujiwara en-aut-mei=Kanta kn-aut-name=藤原寛太 kn-aut-sei=藤原 kn-aut-mei=寛太 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=溶接フューム kn-keyword=溶接フューム en-keyword=気管支肺胞洗浄 kn-keyword=気管支肺胞洗浄 en-keyword=溶接工肺 kn-keyword=溶接工肺 en-keyword=鉄過剰症 kn-keyword=鉄過剰症 en-keyword=ラット kn-keyword=ラット END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=711 end-page=721 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Left heart bypass with left ventricular failure : A special reference to regional myocardial blood flow kn-title=Pusher plate pump varible rate modeによる左心バイパス法の研究―特に，左室局所心筋血流量による検討― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Left heart bypass (LHB) was performed in goats with normal ventricles and in those with LV failure. A pneumatic type pusher-plate pump can be operated in fixed rate, synchronous mode variable pulse rate (VR). In this experiment, the pump was operated in VR, that is, the pusher-plete position signal from a hall effect sensor was utilized to regulate the pump stroke at a constant level, but its rate was allowed to vary automatically depending on the preload and afterload. LHB was performed in 12 goats with normal ventricles. Blood was drained from the left atrium and was returned to the descending aorta. Pump flows averaged 61.3% of cardiac output (CO). When bypass was started, CO decreased from 72.5 to 57.2 ml/min/kg (p<0.005), and LV(max dp/dt) decreased from 3943 to 2989 mmHg/sec (p<0.05). Atrial pressure (AP), A-VDO(2), pulmonary atrial pressure (PAP) and TTI did not show significant changes. Regional myocardial blood flow (RMBF), which was measured using the hydogen gas clearance method, decreased from 128 to 85 ml/min/100g (p<0.05). In 12 goats, LV failure was induced by injecting 0.08 ml/kg of 5N NaOH into the LV free wall. The weight ratio of myocardial necrosis to the LV free wall was 22.7%. Pump flows averaged 52.5% of CO. When bypass was started, CO increased from 45.7 to 61.4 (p<0.005). AP increased from 85.2 to 124 (p<0.001), A-VDO(2) decreased from 6.08 to 4.74 vol% (p<0.01) and PAP decreased from 33.2 to 30.3 (p<0.02). LV(max dp/dt) and TTI did not show significant changes. RMBF, which was determined at normal LV free wall, decreased from 98.6 to 76.1 (p<0.05). These data suggest that LHB is more effective in goats with LV failure than in those with normal ventricles in terms of left ventricular and systemic circulatory support. en-copyright= kn-copyright= en-aut-name=ShiotsuKazuo en-aut-sei=Shiotsu en-aut-mei=Kazuo kn-aut-name=塩津一男 kn-aut-sei=塩津 kn-aut-mei=一男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学第二外科学教室 en-keyword=左心バイパス kn-keyword=左心バイパス en-keyword=心筋局所血流量 kn-keyword=心筋局所血流量 en-keyword=水素ガスクリアランス法 kn-keyword=水素ガスクリアランス法 en-keyword=variable pulse rate kn-keyword=variable pulse rate END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=687 end-page=698 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and characterization of HBs antigen from hepatoma huGK-14 cell line kn-title=ヒト培養肝癌細胞の産生するHBs抗原の精製方法とその物理化学的特性について en-subtitle= kn-subtitle= en-abstract= kn-abstract=HBs antigen was purified from the culture fluid of hepatoma huGK-14 cell line and its physico-chemical properties were studied. The purification consists of following steps: concentration of culture fluid by membrane filtration, affinity column chromatography (anti-HBs monoclonal antibody column and anti-human serum albumin antibody column), and ultracentrifugation (isopycnic centrifugation in CsCl density gradient and rate zonal centrifugation on sucrose gradient). Highly purified (purity>99%) HBs antigen was isolated with an overall yield of about 40%. The HBs antigen showed uniform spherical particles (diameter: 23.2±2.9nm) and had a specific gravity of 1.20g/cm3. The purified HBs antigen yielded, in SDS-PAGE (under reducing conditions), four protein bands with apparent molecular weights of 22,000 and 26,000 (the two major bands), and 44,000 and 47,000. The two proteins of molecular weights of 26,000 and 47,000 are likely to be glycosylated, as these were several fold reduced when the cells were cultured in the presence of Tunicamycin. Amino acid analysis, Edman degradation, carboxypeptidase digestion, and ultraviolet absorption spectrum indicated that the HBs antigen from hepatoma cells is very similar to that derived from human plasma. en-copyright= kn-copyright= en-aut-name=OdaMunehiro en-aut-sei=Oda en-aut-mei=Munehiro kn-aut-name=小田宗宏 kn-aut-sei=小田 kn-aut-mei=宗宏 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源病理学教室 en-keyword=Hepatoma kn-keyword=Hepatoma en-keyword=Cell culture kn-keyword=Cell culture en-keyword=HBs antigen kn-keyword=HBs antigen en-keyword=Purification kn-keyword=Purification en-keyword=Characterization kn-keyword=Characterization END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=699 end-page=709 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of enflurane anesthesia on circulation dynamics and blood catecholamine concentration kn-title=エンフルレン麻酔の循環動態と血中カテコラミン濃度に関する実験的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Effects of enflurane anesthesia were studied on the circulation dynamics and blood catecholamine (CA) concentrations during and after inhalation 2% or 3% enflurane in oxygen in 8 dogs at each concentration. During inhalation, the mean arterial pressure (MAP) and cardiac index (CI) decreased significantly. After stopping inhalation, the MAP recovered rapidly to the control values, but the CI recovered to only 65% of the control values. Immediately after starting inhalation, blood CA concentrations decreased significantly in both groups. After stopping inhalation, blood adrenaline (AD) concentration increased rapidly to 6 and 5 times as the control values in the 2% and 3% groups, respectively. Blood noradrenaline (NA) concentration increased to 3 times as the control values in both groups. One hundred and twenty min after starting inhalation, the dogs did not respond to painful stimulation even in the 2% group. In this study, it has been suggested that the circulation dynamics are suppresed when enflurane has reached more than 0.5 minimum alveolar concentration (MAC) and that the centripetal impulse is thoroughly blocked and CA secretion from the adrenal grand is inhabited at 0.7 MAC of enflurane. en-copyright= kn-copyright= en-aut-name=KajikiHideki en-aut-sei=Kajiki en-aut-mei=Hideki kn-aut-name=梶木秀樹 kn-aut-sei=梶木 kn-aut-mei=秀樹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部麻酔・蘇生学教室 en-keyword=血中エンフルレン濃度 kn-keyword=血中エンフルレン濃度 en-keyword=血中アドレナリン濃度 kn-keyword=血中アドレナリン濃度 en-keyword=血中ノルアドレナリン濃度 kn-keyword=血中ノルアドレナリン濃度 en-keyword=循環動態 kn-keyword=循環動態 END