Conservative treatment is ineffective for ossification of the posterior longitudinal ligament (OPLL) in the thoracic spine, and surgical treatment is indicated for most cases, while such cases are not often experienced. In the present study, the results of surgical management involving mainly posterior decompression for this disease were evaluated clinically. The study included 9 patients (1 man and 8 women) who underwent surgical treatment for OPLL of the thoracic spine between 1984 and 1993. Laminectomy was performed in 5 patients, and laminectomy plus anterior decompression of the OPLL via the posterior approach based on Otsuka's method was performed in 2 patients. In 1 patient, laminoplasty for OPLL of the cervical spine was combined with laminectomy of the symptomatic lesion in the thoracic spine. One patient underwent anterior decompression and fusion. The results were evaluated using the Japanese Orthopaedic Association score (JOA score) and recovery rate. The postoperative follow-up period ranged from 1 year to 10 years and 3 months (mean, 4 years and 6 months). The mean JOA score was 4.8 before surgery and improved to 7.6 at the final examination. This was a mean recovery rate of 50.1%. Symptoms caused by OPLL in the thoracic spine can be alleviated by posterior decompression where OPLL extends from the upper to the middle thoracic spine or extends from the middle to the lower thoracic spine. It seems, however, that OPLL localized to the middle thoracic spine requires anterior decompression.
en-copyright= kn-copyright= en-aut-name=SendaMasuo en-aut-sei=Senda en-aut-mei=Masuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HaradaYoshiaki en-aut-sei=Harada en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakeuchiKazuhiro en-aut-sei=Takeuchi en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakaharaSinnosuke en-aut-sei=Nakahara en-aut-mei=Sinnosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama National Hospital affil-num=5 en-affil= kn-affil=Okayama University en-keyword=ossification of the posterior longitudinal ligament kn-keyword=ossification of the posterior longitudinal ligament en-keyword=thoracic spine kn-keyword=thoracic spine en-keyword=surgical treatment kn-keyword=surgical treatment END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=6 article-no= start-page=305 end-page=310 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Overexpression of c-Met/hepatocyte growth factor receptors in human prostatic adenocarcinoma. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Hepatocyte growth factor (HGF) and c-met proto-oncogene product (c-Met) have varied biological functions in different tissues and have been implicated in mitogenic, motogenic and morphogenic responses in both organ regeneration and carcinogenesis. Some studies have suggested that the overexpression of c-Met and epidermal growth factor receptor (EGFR) are associated with growth advantage, while transforming growth factor-beta receptor II (TGF beta R II) is associated with growth disadvantage of human prostatic adenocarcinoma. However, it is unclear if the expression of c-Met correlates with the expression of EGFR and TGF beta R II, and with the proliferative status of human prostatic adenocarcinoma. Using immunohistochemical staining with anti-c-Met (C-12), anti-EGFR (NCL-EGFR) and anti-TGF beta R II (L-21) antibodies, we determined the frequency of expression of c-MET, EGFR, and TGF beta R II respectively in a series of 134 radical prostatectomy specimens. We evaluated the relationship between the expression of these receptors and clinicopathological characteristics. Overall, c-Met immunostaining was detected in 54 of 134 (40.3%) cases, EGFR in 45 (33.6%) and TGF beta R II in 64 (48.4%). The overexpression of c-Met was significantly more common in poorly differentiated (P < 0.0001) and in the diffusely infiltrated specimens (P < 0.0005). In contrast, TGF beta R II was significantly overexpressed in the well differentiated specimens (P < 0.0001) and associated negatively with c-Met (P < 0.0001). Overall, these data suggest that c-Met/HGF receptor and TGF beta R II overexpression may be involved in the differentiation of human prostatic adenocarcinoma, c-Met with de-differentiation and TGF beta R II with differentiation.
en-copyright= kn-copyright= en-aut-name=InoueKeiji en-aut-sei=Inoue en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ChikazawaMasakazu en-aut-sei=Chikazawa en-aut-mei=Masakazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KarashimaTakashi en-aut-sei=Karashima en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=LiyamaTatsuo en-aut-sei=Liyama en-aut-mei=Tatsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KamadaMasayuki en-aut-sei=Kamada en-aut-mei=Masayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShuinTaro en-aut-sei=Shuin en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=FurihataMutsuo en-aut-sei=Furihata en-aut-mei=Mutsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OhtsukiYuji en-aut-sei=Ohtsuki en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Kochi Medical School affil-num=2 en-affil= kn-affil=Kochi Medical School affil-num=3 en-affil= kn-affil=Kochi Medical School affil-num=4 en-affil= kn-affil=Kochi Medical School affil-num=5 en-affil= kn-affil=Kochi Medical School affil-num=6 en-affil= kn-affil=Kochi Medical School affil-num=7 en-affil= kn-affil=Kochi Medical School affil-num=8 en-affil= kn-affil=Kochi Medical School en-keyword=c-met proto-oncogene product kn-keyword=c-met proto-oncogene product en-keyword=epidermal growth factor receptor kn-keyword=epidermal growth factor receptor en-keyword= transforming growth factor-? recepter ? kn-keyword= transforming growth factor-? recepter ? en-keyword=prostatic adenocarcinoma kn-keyword=prostatic adenocarcinoma en-keyword=immunohisrt chemistry kn-keyword=immunohisrt chemistry END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=6 article-no= start-page=289 end-page=296 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Improvement of sensitivity in HLA-DRB1 typing by semi-nested PCR-RFLP. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific primers. The products of the second round PCRs were digested with restriction endonucleases for the typing of HLA-DRB1 alleles. By this method, HLA-DRB1 typing was possible from 10 pg of genomic DNA extracted from lymphocytes and from 0.5 microliter of 1,000 times diluted blood without DNA extraction. HLA-DRB1 alleles could be typed from a 2-mm long bloodstained cotton thread prepared from 10 times diluted blood and from a 2-mm thread of whole blood bloodstains stored at room temperature for 2 years. From the mixture of blood of two individuals with different genotypes, DRB1 alleles of the minor component were detected down to 1/1,000 of the major component. This semi-nested PCR-RFLP method is useful for HLA-DRB1 typing from extremely small amounts of DNA and from mixed samples.
en-copyright= kn-copyright= en-aut-name=InoueSeiichi en-aut-sei=Inoue en-aut-mei=Seiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkamotoOsamu en-aut-sei=Okamoto en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurakamiHiroki en-aut-sei=Murakami en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IsizuHideo en-aut-sei=Isizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Osaka University en-keyword=polymorphism kn-keyword=polymorphism en-keyword=HLA-DRB1 kn-keyword=HLA-DRB1 en-keyword=polymerase chain reaction kn-keyword=polymerase chain reaction en-keyword=dsmi-nested PCR kn-keyword=dsmi-nested PCR en-keyword=restricton fragment length polymotphism kn-keyword=restricton fragment length polymotphism END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=6 article-no= start-page=297 end-page=303 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Embedding of Laboratory Wastes in Clay or Concrete Blocks, with Special Reference to Baking Osmic Acid and Cacodylic Acid Wastes with Clay en-subtitle= kn-subtitle= en-abstract= kn-abstract=Liquid laboratory waste containing osmic acid and cacodylic acid was mixed with potter's clay or hydraulic cement. The clay-waste product was kneaded into blocks and baked in a klin (1,200-1,400 degrees C). The cement-waste product was allowed to harden into concrete blocks. Some of the baked clay blocks and concrete blocks were ground, and immersed in 1 N NaOH or 10% HCI solutions for 3-6 months. X-ray microanalysis of the dried samples of these solutions showed that no leakage of osmium and arsenic occurred in the baked clay embedding, and that some leakage of these agents occurred in the concrete embedding. The present study indicates that the baked clay embedding method is useful for safe storage of dangerous laboratory wastes. Additional experiments suggested that glass embedding is also useful for safe storage of laboratory wastes or harmful metals.
en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurakamiTetsuro en-aut-sei=Murakami en-aut-mei=Tetsuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamanaSeizo en-aut-sei=Yamana en-aut-mei=Seizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy en-keyword=laboratory waste kn-keyword=laboratory waste en-keyword=osmic acid kn-keyword=osmic acid en-keyword=cacodylic acid kn-keyword=cacodylic acid en-keyword=clay-embedding kn-keyword=clay-embedding en-keyword=cement-embedding kn-keyword=cement-embedding en-keyword=baking kn-keyword=baking END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=6 article-no= start-page=285 end-page=288 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Continuous calcium channel blocker infusion in experimentally induced acute pancreatitis: effects on pancreas and liver function. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this study we examined the effects of continuous calcium channel blocker (CCB) infusion on pancreatic duct-ligated acute pancreatitis (AP) in rabbits. Thirty rabbits were used for this study. Animals in group 1 (n = 10), which served as a control group, underwent dummy operations and received 0.5 microliter/h normal saline via the internal jugular vein. Animals in group 2 (n = 10) with artificially-induced pancreatitis received the same dosage of saline in the same manner. Animals in group 3 (n = 10) with artificially-induced pancreatitis received 180 micrograms/kg/h CCB (Verapamil) via the jugular vein starting from just before pancreatic duct ligation. AP histology score, plasma amylase levels and liver function tests were measured after 48 h. Verapamil infusion did not prevent the rise in plasma amylase levels, nor did it prevent pancreatic inflammation and damage. Serum levels of serum glutamate pyruvate transaminase, serum glutamate oxalacetate transaminase and alkaline phosphatase were significantly elevated in group 2 and significant reductions were seen in the Verapamil treated animals (group 3). The findings in this study imply that a continuous 180 micrograms/kg/h dose Verapamil infusion does not ameliorate the pathogenesis of pancreatitis induced by ligation of pancreatic duct but do not rule out a dose-dependent protective effect. Meanwhile, the lowering of liver function test scores should be considered the beneficial effect of CCBs, and this should be investigated in further studies.
en-copyright= kn-copyright= en-aut-name=SoranAtilla en-aut-sei=Soran en-aut-mei=Atilla kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YucelErdem en-aut-sei=Yucel en-aut-mei=Erdem kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=CinerIsmail en-aut-sei=Ciner en-aut-mei=Ismail kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=CinerLeyla en-aut-sei=Ciner en-aut-mei=Leyla kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Ankara Numune Teaching Hospital affil-num=2 en-affil= kn-affil=Ankara Numune Teaching Hospital affil-num=3 en-affil= kn-affil=Ankara Numune Teaching Hospital affil-num=4 en-affil= kn-affil=Ankara Numune Teaching Hospital en-keyword=acute pancreatitis kn-keyword=acute pancreatitis en-keyword=duct ligation kn-keyword=duct ligation en-keyword=calcium channel blocker kn-keyword=calcium channel blocker en-keyword=liver function test kn-keyword=liver function test END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=6 article-no= start-page=325 end-page=329 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Can POSSUM, a Scoring System for Perioperative Surgical Risk, Predict Postoperative Clinical Course ? en-subtitle= kn-subtitle= en-abstract= kn-abstract=POSSUM, a Physiological and Operative Severity Score for the enUmeration of Mortality and morbidity, is a scoring system which assesses perioperative surgical risks (Copeland GP et al.: Br J Surg, 1991, Vol 78, 356-360). The POSSUM scoring system consists of two categories of assessment to assess the risk of surgery. A 12-factor (age, cardiac status, pulse rate, systolic blood pressure, respiratory status, Glasgow Coma Score, serum concentration of urea, potassium and sodium, hemoglobin concentration, white cell count and findings on electrocardiography) and 4-grade physiological score (PS) were developed. This was combined with a 6-factor (type of surgical procedure, number of procedures, blood loss, peritoneal soiling, presence of malignancy and mode of surgery) and 4-grade operative severity score (OSS). The present paper attempts to validate it retrospectively. Postoperative hospitalization period and duration of antibiotics administration were both significantly correlated with OSS, but not with PS. These results suggest that the POSSUM scoring system is useful for predicting the postoperative clinical course.
en-copyright= kn-copyright= en-aut-name=GotohdaNaoto en-aut-sei=Gotohda en-aut-mei=Naoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ItanoSatoshi en-aut-sei=Itano en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HorikiSadayuki en-aut-sei=Horiki en-aut-mei=Sadayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FujiwaraToshiya en-aut-sei=Fujiwara en-aut-mei=Toshiya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SaitoShinya en-aut-sei=Saito en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HizutaAkio en-aut-sei=Hizuta en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IsozakiHiroshi en-aut-sei=Isozaki en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TakakuraNorihisa en-aut-sei=Takakura en-aut-mei=Norihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TeradaNorihiko en-aut-sei=Terada en-aut-mei=Norihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Terada General Hospital affil-num=4 en-affil= kn-affil=Terada General Hospital affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Terada General Hospital affil-num=11 en-affil= kn-affil=Okayama University en-keyword=surgical risk kn-keyword=surgical risk en-keyword=Physiological and Operative Severity Source for the enUmeration of Mortality and morbidity kn-keyword=Physiological and Operative Severity Source for the enUmeration of Mortality and morbidity END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=6 article-no= start-page=311 end-page=318 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Binding Specificities of Lectins to Immobilized Glycoproteins and Oligosaccharides Differ from Those of Immobilized Lectins to Oligosaccharides en-subtitle= kn-subtitle= en-abstract= kn-abstract=The carbohydrate-binding specificities of lectins in solution to glycoproteins and neoglycolipids immobilized on a solid phase were analyzed in order to establish a simple, rapid method for structural analysis of the carbohydrate moieties of small amounts of individual glycoproteins blotted on membrane. Eight glycoproteins containing typical O-linked tetrasaccharides or a series of typical N-linked oligosaccharides of the high-man-nose type, hybrid type, and complex type and 6 neoglycoproteins containing mono- or di-saccharides were dot blotted on membranes and the membranes were then reacted with 8 kinds of horseradish peroxidase-conjugated lectins before and after heat treatment. Neoglycolipids containing the glycoprotein-derived oligosaccharides immobilized on a thin layer chromatography plate were also reacted with lectins. The heat treatment of the membrane increased lectin reactivity toward the glycoproteins. The carbohydrate-binding behavior of lectins, Phaseolus vulgaris erythroagglutinin, wheat germ agglutinin, and concanavalin A in solution toward glycoproteins and neoglycolipids immobilized on a solid phase differed from that of immobilized lectins toward oligosaccharides in solution. This difference should be noted in lectin detection of specific carbohydrates of individual glycoproteins on membrane.
en-copyright= kn-copyright= en-aut-name=TangWei en-aut-sei=Tang en-aut-mei=Wei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiuraTakehiko en-aut-sei=Miura en-aut-mei=Takehiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakataMunehiro en-aut-sei=Nakata en-aut-mei=Munehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KojimaNaoya en-aut-sei=Kojima en-aut-mei=Naoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MizuochiTsuguo en-aut-sei=Mizuochi en-aut-mei=Tsuguo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Tokai University affil-num=2 en-affil= kn-affil=Tokai University affil-num=3 en-affil= kn-affil=Tokai University affil-num=4 en-affil= kn-affil=Tokai University affil-num=5 en-affil= kn-affil=Tokai University en-keyword=glycoprotein kn-keyword=glycoprotein en-keyword=lectins kn-keyword=lectins en-keyword=lectin binding specificity kn-keyword=lectin binding specificity en-keyword=neoglycolipid kn-keyword=neoglycolipid en-keyword=oligosaccharide kn-keyword=oligosaccharide END