JaLCDOI 10.18926/AMO/31302
FullText URL fulltext.pdf
Author Bozkir, Memducha Gulhal| Dere, Fahri| Mete, Ufuk Ozgu| Kaya, Mehmet|
Abstract <p>The ultrastructural and biochemical changes in the brain tissue of 20 mice were studied. The mice, separated into acute and chronic groups, were injected with phorbol myristate acetate (PMA) to trigger the release of free radicals. Malondialdehyde measurement and electron microscopic examination were applied for the evaluation of the effects of the free radicals. The level of lipid peroxide in the chronic PMA group was found to be significantly higher than it was in the acute PMA group (P &#60; 0.005). An electron microscopic examination of the acute group revealed disruption of the mitochondrial cristae and dilatation of the endoplasmic reticulum in the neurons. Myelin sheaths of the nerve fibers exhibited focal structural changes. Neurons and neuroglial cells in the chronic group, however, exhibited distinct ultrastructural alterations. The ultrastructural and biochemical findings showed that free radicals lead to brain damage.</p>
Keywords free radical phorbol myristate acetate malodialdehyde brain ultrastruture
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 205
End Page 209
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781271
Web of Science KeyUT 000075623600005
JaLCDOI 10.18926/AMO/31301
FullText URL fulltext.pdf
Author Yano, Akemi| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.</p>
Keywords DNA polymorphism haptoglobin polymerase chain reaction allele-specific amplification personal identification
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 173
End Page 181
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781267
Web of Science KeyUT 000075623600001
JaLCDOI 10.18926/AMO/31300
FullText URL fulltext.pdf
Author Yoshida, Atushi| Sotozono, Masaaki| Nakatou, Tatsuaki| Okada, Yoshio| Tsuji, Takao|
Abstract <p>Thomsen-Friedenreich antigen (T antigen) has been supposed to be a cancer-specific carbohydrate antigen. We have previously shown that one third of the Japanese population normally expressed T antigen in gastric surface epithelia and the other two thirds expressed fucosyl-T antigen. Their sialylation and blocked-synthesis were associated with diseased conditions. In the present study, we studied gastric surface epithelial expression of monosaccharide antigen Tn, i.e., a precursor of T antigen, and sialyl-Tn. Normal fundic and pyloric epithelia, respectively, expressed Tn supranucleally and cytoplasmically, but did not express sialyl-Tn. In the intestinal metaplasias and intestinal-type adenomas, goblet cells expressed sialyl-Tn in their vacuoles, and absorptive cells expressed Tn apically. In gastric-type adenomas, Tn, but not sialyl-Tn, was detected. Intestinal-type cancers expressed Tn and sialyl-Tn more often than the diffuse-type cancers. Five cancers did not express Tn, sialyl-Tn, or the T-related antigens. In these, four were diffuse-type cancers. We concluded that: a) normal gastric epithelial cells express Tn; b) metaplastic differentiation is associated with sialylation of Tn and c) expression of Tn and sialyl-Tn is depressed in the gastric cancers. </p>
Keywords Tn immunohistochemistru
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 197
End Page 204
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781270
Web of Science KeyUT 000075623600004
JaLCDOI 10.18926/AMO/31299
FullText URL fulltext.pdf
Author Piao, Da Xun| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>Origins and distribution of the human inferior phrenic arteries were studied by dissecting 68 Japanese adult cadavers. The inferior phrenic arteries were usually observed as paired (left and right) vessels. Their origins were summarized as follows: a) the aorta itself (85/138 cases, 61.6%), b) the ventro-visceral arteries (celiaco-mesenteric system of the aorta) including the celiac trunk (39/138 cases, 28.2%) and the left gastric artery (4/138 cases, 2.9%), and c) the latero-visceral arteries (adreno-renal system of the aorta) including the middle adrenal artery (4/138 cases, 2.9%) and the renal artery (6/138 cases, 4.3%). The left and right arteries occasionally originated in common trunk from the aorta, celiaco-mesenteric system or adreno-renal system (22/138 cases, 15.9%). A typological diagram explaining these variations is given. The inferior phrenic arteries, especially the left ones, sometimes issued visceral or esophageal branches. This fact indicates that the inferior phrenic arteries are homologous with the celiac trunk and mesenteric arteries. It is further discussed that the celiac trunk and mesenteric arteries are originally paired vessels, through introduction of our previous typological diagram of the abdominal arteries.</p>
Keywords inferior phrenic artery esophageal branch typology of abdominal arteries
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 189
End Page 196
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781269
Web of Science KeyUT 000075623600003
JaLCDOI 10.18926/AMO/31298
FullText URL fulltext.pdf
Author Akisu, Mete| Kultursay, Nilgun| Coker, Lsil| Huseyinov, Afig|
Abstract <p>Recent data suggested that platelet-activating factor (PAF) could play a pathophysiologically important role in the progression of hypoxic-ischemic brain injury. We investigated brain tissue PAF concentration in the hypoxic-ischemic brain of immature rats. Endogenous PAF concentration in brain tissue showed a marked increase in hypoxic-ischemic pups (Group 1, 85.6 +/- 15.5 pg/mg protein) when compared to that of the control (9.1 +/- 3.1 pg/mg protein). In addition, we studied the effects of pretreatment with L-carnitine (5 days and 2 h before the hypoxia) on endogenous PAF concentration in the hypoxic-ischemic brain. Endogenous PAF concentration in the short-term pretreatment group (Group 2, 81.6 +/- 9.7 pg/mg protein) was not different than in Group 1 rat pups. However, a significantly decreased PAF concentration was found in the group of pups that received carnitine pretreatment for 5 days (Group 3, 30.5 +/- 11.0 pg/mg protein). These results indicate that PAF is an important mediator in the immature rat model of cerebral hypoxic-ischemic injury. The suppressor effect of L-carnitine on PAF production may give new insight into the treatment of hypoxic-ischemic brain injury.</p>
Keywords perinatal asphyxia rat carnitine ischmia brain platelet-activating factor
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 183
End Page 187
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781268
Web of Science KeyUT 000075623600002
JaLCDOI 10.18926/AMO/31297
FullText URL fulltext.pdf
Author Mahmood, Sabina| Taketa, Kazuhisa| Imai, Kaori| Kajihara, Yasumasa| Imai, Shigeki| Yokobayashi, Tsuneo| Yamamoto, Shinichiro| Sato, Mikio| Omori, Hiroyuki| Manabe, Koji|
Abstract <p>We studied the association of fatty liver with subcutaneous and visceral obesity in 46 male and 36 female patients with body mass index (BMI) over 22 kg/m2. The correlation coefficient between the ratio of the visceral adipose tissue to the subcutaneous adipose tissue (V/S) and the computed tomography (CT) number of the liver was -0.299 (P &#60; 0.05) and that between the V/S ratio and the ratio of the CT number of the liver to that of the spleen (CT-L/CT-S) was -0.335 (P &#60; 0.05) in the males. Partial correlation analysis after making correction for BMI showed an increased correlation coefficient of -0.485 (P &#60; 0.05) between the V/S ratio and the CT-L/CT-S ratio in the males. The odds ratio in the males for CT-L/CT-S below 1.0 and V/S above 1.0 was 3.25 with a 95% confidence interval of 1.02 to 9.39. No such association between the V/S ratio and the CT-L/CT-S ratio was present in the female patients. Multiple regression analysis with serum level of alanine aminotransferase, a marker of fatty liver, as an independent variable revealed a partial regression coefficient of -17.7 for CT-L/CT-S (P &#60; 0.05) in the males and -21.7 (P &#60; 0.05) in the females, validating the CT-L/CT-S ratio as an index of fatty liver. The results indicate the association of fatty liver as determined by the CT-L/CT-S ratio with visceral obesity in males. </p>
Keywords fatty liver visceral obesity computed tomography number
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 225
End Page 231
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781273
Web of Science KeyUT 000075623600007
JaLCDOI 10.18926/AMO/31296
FullText URL fulltext.pdf
Author Takeuchi, Kazuhiro| Inoue, Hajime| Yokoyama, Yoshiki| Senda, Masuo| Ota, Yusuke| Abe, Nobuhiro| Nishida, Keiichiro|
Abstract <p>We studied the magnetic resonance imaging (MRI) of 120 knees in 86 rheumatoid arthritis (RA) patients and of 14 unaffected knees in 12 control cases. We also developed a scoring system as a quantitative analysis method. We divided the MRI into 10 items, and classified the severity of the symptoms into 4 grades (score 0 to 3). The average total score increased according to the radiographic grade. Soft tissue lesions were clearly detected, even in the early stages of RA. Items such as synovial proliferation showed a high score even in the early stages, suggesting that it was the initial symptom of RA. The score also showed a correlation with the inflammatory signs. These results suggest that this scoring system is very sensitive and yields a good reflection of RA activity. We demonstrated that this system is simple and convenient for routine diagnostic use. We further demonstrated that it is useful for following the advancement of RA and for evaluating the response to treatment.</p>
Keywords rheumatoid arthritis magnetic resonance imaging scoring system synovial membrane
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 211
End Page 224
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781272
Web of Science KeyUT 000075623600006