JaLCDOI 10.18926/AMO/31343
FullText URL fulltext.pdf
Author Suemaru, Katsuya| Kawakami, Yasuhiro| Araki, Hiroaki| Gomita, Yutaka| Tanizaki, Yoshiro|
Abstract <p>The effect of 6-hydroxydopamine on repeated nicotine-induced tail-tremor was investigated in rats. Tail-tremor induced by nicotine (0.5 mg/kg/day, subcutaneously) became more pronounced in intensity with daily administration for 9 days. Rats pretreated with 6-hydroxydopamine (250 micrograms, intracerebroventricularly) showed almost the maximum degree of tail-tremor during the whole experimental period. However, in rats pretreated with 6-hydroxydopamine plus desipramine, enhancement of tail-tremor was slight in the beginning but increased with the daily nicotine administration. Fourteen-day administration of nicotine did not result in significant changes in noradrenaline and dopamine levels in the cortex, hypothalamus, striatum and nucleus accumbens. These results suggest that nicotine-induced tail-tremor is associated with the supersensitivity of postsynaptic catecholaminergic receptors in the central nervous system, and that the noradrenergic system may be more important than the dopaminergic system in this phenomenon.</p>
Keywords nicotine tail-tremor 6-hydroxydopamine noradrenaline dopamine
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 49
End Page 53
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548994
Web of Science KeyUT 000072264100007
JaLCDOI 10.18926/AMO/31342
FullText URL fulltext.pdf
Author Ohta, Masahiro| Kawahara, Nobuaki| Liu, Miao| Taketa, Kazuhisa| Kudo, Takafumi| Taga, Hiroko|
Abstract <p>Affinity electrophoresis of human alpha-fetoprotein (AFP) in amniotic fluid from pregnant women between 6 to 42 weeks of gestation and in the serum of a yolk sac tumor was performed with concanavalin A (Con A), lentil lectin (LCA), erythroagglutinating phytohemagglutinin (E-PHA) and Allomyrina dichotoma lectin (allo A). Separated AFP bands were detected by sensitive antibody-affinity blotting. In the first trimester, amniotic fluid AFP showed elevated percentages of Con A-nonreacting AFP (AFP-C1) and LCA weakly-reacting AFP (AFP-L2) as previously reported. Additionally, high percentages of E-PHA strongly-reacting AFP (AFP-P5) and E-PHA-reacting AFP (AFP-P4) were observed. E-PHA-nonreacting AFP (AFP-P1), E-PHA weakly-reacting AFP (AFP-P3f), allo A-nonreacting AFP (AFP-A1) and asialo-AFP, AFP-A1 s, were present only in amniotic fluids from 6 to 17 weeks of gestation. With advancing gestation, percentages of AFP-C1, AFP-P4 and AFP-P5 decreased and AFP-L2, AFP-P3f, AFP-A1, and AFP-A1 s disappeared by the end of 18 weeks. The glycoforms of serum AFP of the yolk sac tumor resembled those of amniotic fluid AFP in the early gestational stages. </p>
Keywords ?-fetoprotein affinity electrophoresis lectin amniotic fluid yolk sac tumor
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 27
End Page 33
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548991
Web of Sience KeyUT 000072264100004
JaLCDOI 10.18926/AMO/31341
FullText URL fulltext.pdf
Author Mizushima, Takaaki| Ochi, Koji| Seno, Toshinobu| Matsumura, Naoki| Harada, Hideo|
Abstract We simultaneously assessed gastric emptying and gallbladder contraction after oral administration of a liquid meal by noninvasive ultrasonography in 17 patients with chronic pancreatitis (CP) and in 17 healthy controls. Gastrointestinal (GI) transit was also assessed by a noninvasive radioopaque marker method. Exocrine pancreatic function was evaluated by analyzing pure pancreatic juice and by analyzing the autonomic nervous system by cardiovascular reflex tests. Patients with CP showed impaired gallbladder contraction at 15 min and hastened gastric emptying. The cause of the former is unclear, whereas the latter was closely related with decreased pancreatic lipase output, but not with autonomic dysfunction. GI transit time did not differ between controls and patients with CP. In conclusion, we succeeded in clearly demonstrating impaired gallbladder contraction and hastened gastric emptying in patients with CP by a single noninvasive test, ultrasonography. We also revealed for the first time that hastened gastric emptying is associated with insufficient pancreatic lipase output.
Keywords chronic pancreatits gastric emptying gallbladder motility colonic transit lipase output
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 55
End Page 61
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548995
Web of Science KeyUT 000072264100008
JaLCDOI 10.18926/AMO/31340
FullText URL fulltext.pdf
Author Ozaki, Toshifumi| Nakatsuka, Yoichi| Kawai, Akira| Akazawa, Hirofumi| Kunisada, Toshiyuki| Inoue, Hajime|
Abstract <p>There are few reports on skeletal reconstruction using the bone transport technique to repair bone defects caused by resections of tumors associated with osteosarcoma. We attempted to reconstruct a 23 cm bone defect after resection of an osteosarcoma of the left femur, and succeeded in gaining 17 cm by bone transport. Five years after surgery, this patient remains alive without metastasis or local recurrence.</p>
Keywords osteosarcoma bone transport reconstruction
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 67
End Page 70
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548997
Web of Sience KeyUT 000072264100010
JaLCDOI 10.18926/AMO/31339
FullText URL fulltext.pdf
Author Pu, Jian| Nishida, Keiichiro| Inoue, Hajime| Asahara, Hiroshi| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>Mast cells in osteoarthritic and rheumatoid arthritic synovial tissues of the human knee.</p>
Keywords mast cell count osteoaethritis rheumatoid arthritis hydroarthrosis synovial membrance
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 35
End Page 39
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548992
Web of Sience KeyUT 000072264100005
JaLCDOI 10.18926/AMO/31338
FullText URL fulltext.pdf
Author Takagoshi, Hidekazu| Hashizume, Hiroyuki| Nishida, Keiichiro| Masaoka, shunji| Asahara, Hiroshi| Inoue, Hajime|
Abstract <p>The fibrous components of the metacarpophalangeal (MP) joint including the palmar plate, the collateral ligament and the dorsal plate were studied with particular attention paid to the fibrous structure of the fibrous tendon sheath and the deep transverse metacarpal ligament. The tough fibrillar structure around the MP joint, especially the force nucleus, consisted of three types of mixed fibers: the fibrous tendon sheath of the A1 pulley, the deep transverse metacarpal ligament, and the palmar plate. The tendon sheath was located on the ulnar side in the index and middle fingers, on the central position in the ring finger, and on the radial side in the little finger. These fibrous connections among the fingers formed a transverse arch in the hand. The palmar plate of the MP joint was relatively rigid and appears to function as a cushion when flexed. A fold-like protrusion of the synovial layer of the palmar plate of the MP joint had a meniscoid function, which was larger than that of the proximal interphalangeal joint. The capsule of the MP joint was thicker at the dorsal area, forming a dorsal plate, which is a sliding floor of the extensor mechanism and has a meniscoid function for joint congruity. The main lateral stabilizer consisted of collateral ligaments and accessory collateral ligaments anchored to the palmar plate. These structures act together as a &#34;phalangeal cuff&#34;, connecting the proximal phalanx to the metacarpal head and sta</p>
Keywords metacarpophalangeal joint collagen framework
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 19
End Page 26
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548990
Web of Science KeyUT 000072264100003
JaLCDOI 10.18926/AMO/31337
FullText URL fulltext.pdf
Author Dey, Ashoka| Yokota, Kenji| Kobayashi, Keita| Oguma, Keiji| Hirai, Yoshikazu| Akagi, Tadaatsu|
Abstract <p>Helicobacter pylori (H. pylori) infection in the stomach is etiologically closely associated with chronic active gastritis, peptic ulcer, gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma. In this study, we examined the antibody responses and cytokine profiles of three strains of mice (BALB/c, C3H/He, and C57BL/6) infected with H. pylori. Following this, correlations between host-immune reactions and intensity of inflammation were analyzed. H. pylori (ATCC43504) was intragastrically administered once a week to the mice from 4 weeks of age, and they were sacrificed at the ages of 4 and 7 months. In these mice, we examined the histology of the stomach, antibody titers against H. pylori, and serum levels of cytokines (IL-4, IL-10, TNF-alpha, IL-2 and Interferon-gamma). In BALB/c mice, inflammation of the stomach was minimal. Inflammation was observed in 63.6% of C57BL/6 mice and 33.3% of C3h/He mice. In C57BL/6 and C3H/He mice, all the cytokines tended to increase. In contrast, BALB/c mice were inactive in cytokine production except for IL-2. Two C3H/He mice developed severe inflammation with lymph follicles; one showed a response largely typical of Th-1, and the other showed a response largely typical of Th-2. Although a definite correlation was not shown between Th-1/Th-2 response evaluated by cytokine production and intensity of inflammation, it appears that in H. pylori-induced inflammation both cell-mediated (Th-1) and humoral (Th-2) immunity play a role in pathogenesis.</p>
Keywords Helicobacter pylori cytokine humoral immunity cell-mediated immunity gastritis
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 41
End Page 48
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548993
Web of Science KeyUT 000072264100006
JaLCDOI 10.18926/AMO/31336
FullText URL fulltext.pdf
Author Ito, Akira| Moriya, Fumio| Ishizu, Hideo|
Abstract <p>To establish a method for estimating the time between the last consumption of alcohol and death, we examined the ethanol levels in body fluids and tissues of rats that had been orally administered 1 g/kg ethanol. We observed the following relationships between ethanol levels in the cardiac blood (blood in the heart itself), vitreous humor, and urine: cardiac blood &#62; vitreous humor &#62; urine at 10 min (early absorption stage); vitreous humor &#62; cardiac blood &#62; urine from 20 to 50 min (late absorption stage); vitreous humor &#62; urine &#62; cardiac blood from 60 to 120 min (distribution stage); and urine &#62; vitreous humor &#62; cardiac blood at 180 min (excretion stage). It was also observed that, in cases of death immediately following drinking, ethanol levels in the stomach contents are very high, and the following ratios of ethanol levels were observed: skeletal muscle to cardiac blood--less than 1; liver to cardiac blood--around 1. buccal mucosa to cardiac blood-greater than 1. These ratios at equilibrium after drinking were around 1, lower than 1 and around 1, respectively. We also measured alcohol levels in the cardiac blood, urine, vitreous humor and stomach contents of nine cadavers who had consumed alcohol prior to death. The relationships between the time since last consumption of alcohol and relative ethanol levels in these specimens were in good accordance with the results of the animal experiments. </p>
Keywords toxicology ethyl alcohol ethanol in cadavers tissue distribution of ethanol time between drinking and death
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 8
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548988
Web of Science KeyUT 000072264100001
JaLCDOI 10.18926/AMO/31335
FullText URL fulltext.pdf
Author Ozaki, Toshifumi| Nakatsuka, Yoichi| Kawai, Akira| Akazawa, Hirofumi| Kunisada, Toshiyuki| Inoue, Hajime|
Abstract <p>There are few reports on skeletal reconstruction using the bone transport technique to repair bone defects caused by resections of tumors associated with osteosarcoma. We attempted to reconstruct a 23 cm bone defect after resection of an osteosarcoma of the left femur, and succeeded in gaining 17 cm by bone transport. Five years after surgery, this patient remains alive without metastasis or local recurrence.</p>
Keywords osteosarcoma bone transport reconstruction
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 67
End Page 70
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548997
Web of Sience KeyUT 000072264100010
JaLCDOI 10.18926/AMO/31334
FullText URL fulltext.pdf
Author Kan, Shin| Moriya, Fumio| Ishizu, Hideo|
Abstract <p>The main purpose of this study was to evaluate the inhibitory effects of 5-fluorouracil antineoplastics, cephem antibiotics containing the methyltetrazolylthiol (MTT) group and antidiabetics on aldehyde dehydrogenase (ALDH) activity in vivo and in vitro. In in vivo experiments, rats were given a 100 mg/kg dose of drugs (10 mg/kg for glibenclamide) orally or intraperitoneally. When each drug was administered singly immediately after an oral administration of 1.5 g/kg ethanol, only carmofur, an antineoplastic, produced marked increases in blood acetaldehyde concentrations. This action was also noted when ethanol was ingested 15 h after administration. The remaining drugs did not increase blood acetaldehyde concentrations. When rats were treated with carmofur at 12 h intervals for 3 consecutive days and were given 1.5 g/kg ethanol after the final treatment, blood acetaldehyde concentrations were elevated more significantly than with a single administration of carmofur. Furthermore, daily administration of cephem antibiotics containing the MTT group, latamoxef, cefamandole, cefoperazone and cefbuperazone, significantly increased blood acetaldehyde concentrations. Daily administration of sulfonylurea antidiabetics, chlorpropamide and acetohexamide, slightly increased blood acetaldehyde concentrations. Drugs causing increases in blood acetaldehyde concentrations when administration was combined with ethanol ingestion also inhibited ALDH activity in vitro. The results of the in vitro experiments roughly correlated with those of the in vivo experiments. The inhibitory effects of drugs on ALDH activity were in the following order: carmofur &#62;&#62; cephem antibiotics containing the MTT group &#62; sulfonylurea antidiabetics.</p>
Keywords toxicology acetaldehyde aldehyde dehydrogenase disulfiram-like reaction carmofur
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 9
End Page 17
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548989
Web of Science KeyUT 000072264100002
JaLCDOI 10.18926/AMO/31333
FullText URL fulltext.pdf
Author Akdeniz, Hayrettin| Irmak, Hasan| Seckinli, Tahir| Buzgan, Turan| Demiroz, Ali Pekcan|
Abstract <p>The hematological findings of 233 patients with brucellosis are presented and the possible pathologies discussed. Anemia was present in 128 patients (55%), leukopenia in 49 (21%) and thrombocytopenia in 59 (26%). Bone marrow aspirates of 18 patients (8%) with pancytopenia were examined. The bone marrow was hypercellular in 15 and normocellular in 3 patients. Granulomatous lesions were detected in 12 cases (67%), and slight to moderate cytophagocytosis of erythrocytes, granulocytes and platelets existed in all patients. Blood cell counts reverted to normal within 2-3 weeks of initiating chemotherapy with recovery from the disease.</p>
Keywords brucellosis hematology pancytopenia disseminated intravascular coagulation
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 63
End Page 65
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548996
Web of Science KeyUT 000072264100009