In this study, we established the surgical procedure and postoperative care of multivisceral transplantation (MVTX) in pigs, and examined the functional changes and rejection pattern of transplanted organs in MVTX. Twenty-two MVTXs were performed without immunosuppression, and nine cases (41%) that survived for 5 days or more after MVTX were used for evaluation. Rejection in grafts including the liver, pancreas, and gastrointestinal tract were assessed histopathologically. On day 5 after transplantation, the duodenum and small bowel already showed signs of mild rejection. On the other hand, in the liver, pancreas and stomach, rejection occurred later and was still mild on day 16. Hepatic rejection in MVTX appeared to occur later than in simple liver transplantation (LTX). These results showed that the susceptibility to rejection of individual visceral organs varies.
en-copyright= kn-copyright= en-aut-name=MitsuokaShintaro en-aut-sei=Mitsuoka en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=multivisceral transplantation kn-keyword=multivisceral transplantation en-keyword=allograft kn-keyword=allograft en-keyword=rejection kn-keyword=rejection en-keyword=pigs kn-keyword=pigs END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=271 end-page=274 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of cigarette smoke on lipid peroxidation and liver function tests in rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effect of cigarette smoke on organ weights, lipid peroxidation and plasma biochemical parameters was investigated in male Wistar rats. Daily exposure (for 20 min twice a day) to cigarette smoke for 27 days caused a significant decrease in liver weight and a significant increase in lung weight. The smoke-exposure group showed increased lipid peroxidation in the liver, but not in the lung. In the smoke-exposure group, the GOT, gamma-GTP, total bilirubin and LDH values were significantly higher than those in the control group, while the plasma glucose value was significantly lower. These results suggest that cigarette smoking might induce liver injury by enhancing lipid peroxidation.
en-copyright= kn-copyright= en-aut-name=WatanabeKazuhide en-aut-sei=Watanabe en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EtoKoehi en-aut-sei=Eto en-aut-mei=Koehi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FurunoKatsushi en-aut-sei=Furuno en-aut-mei=Katsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoriTakaaki en-aut-sei=Mori en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Kawatetsu Mizushima Hospital affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=cigarette smoking kn-keyword=cigarette smoking en-keyword=lipid peroxidation kn-keyword=lipid peroxidation en-keyword=liver function kn-keyword=liver function en-keyword=rats kn-keyword=rats END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=231 end-page=236 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Possible Roles of Nitric Oxide and Vasoactive Intestinal Polypeptide on Relaxation Induced by Isoprenaline in Isolated Muscle Strips of the Mouse Gastric Fundus en-subtitle= kn-subtitle= en-abstract= kn-abstract=The possible role of nitric oxide (NO) and vasoactive intestinal polypeptide on isoprenaline-induced relaxation of the mouse longitudinal gastric fundal strips precontracted with 5.4 x 10(-7) M carbachol was investigated. Isoprenaline (5 x 10(-7) M, 10(-6) M and 5 x 10(-6) M) produced a concentration-dependent relaxations. NG-nitro L-arginine (10(-4) M) partly inhibited isoprenaline-induced relaxation. The inhibitory action of NG-nitro L-arginine was reversed by 4 x 10(-4) M L-arginine but not by 4 x 10(-4) M D-arginine. NG-nitro L-arginine (10(-4) M) did not affect the relaxation caused by sodium nitroprusside (10(-6) M). Vasoactive intestinal polypeptide antibody 7913 (1:160 dilution) partly inhibited isoprenaline-induced relaxation. This inhibition was greater on the response to the higher isoprenaline concentration (5 x 10(-6) M) than to the lower concentration (10(-6) M). The combination of vasoactive intestinal polypeptide antibody and NG-nitro L-arginine significantly enhanced the inhibition on 10(-6) M isoprenaline action. These results suggest that nitric oxide and vasoactive intestinal polypeptide may partly contribute to the relaxation induced by isoprenaline in the mouse gastric fundus precontracted with carbachol.
en-copyright= kn-copyright= en-aut-name=OgulenerNuran en-aut-sei=Ogulener en-aut-mei=Nuran kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=LarabalEda en-aut-sei=Larabal en-aut-mei=Eda kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=BaysalFiruz en-aut-sei=Baysal en-aut-mei=Firuz kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=DikmenAtilla en-aut-sei=Dikmen en-aut-mei=Atilla kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Cukurova University affil-num=2 en-affil= kn-affil=Cukurova University affil-num=3 en-affil= kn-affil=Cukurova University affil-num=4 en-affil= kn-affil=Cukurova University en-keyword=isoprenaline kn-keyword=isoprenaline en-keyword= NG-nitro L-arginine(L-NOARG) kn-keyword= NG-nitro L-arginine(L-NOARG) en-keyword= L-arginine(L-ARG) kn-keyword= L-arginine(L-ARG) en-keyword= D-arginine(D-ARG) kn-keyword= D-arginine(D-ARG) en-keyword=vasoactive intestinal polypeptide (VIP) antibody 7913 kn-keyword=vasoactive intestinal polypeptide (VIP) antibody 7913 en-keyword=isolated mouse gastric fundus kn-keyword=isolated mouse gastric fundus END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=247 end-page=252 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Diuretic effect of cilazapril and dopamine system in the spontaneously hypertensive rat. en-subtitle= kn-subtitle= en-abstract= kn-abstract=<p>To assess the role of the kidney dopamine system on the diuretic state induced by angiotensin-converting enzyme (ACE) inhibitors, we examined the changes in urinary excretion and plasma level of dopamine, and kidney dopamine receptors in spontaneously hypertensive rats (SHR) treated with cilazapril, an ACE inhibitor. We administered cilazapril 10 mg/kg orally to 13-week-old SHR daily for 21 days (CILAZA group). Systolic blood pressure was significantly decreased in the CILAZA group on Day 6 compared with that in vehicle-treated SHR (control group). The urine volume was three- to fivefold higher in the CILAZA group, and total urinary dopamine secretion was also increased compared with the control group. There was no significant difference in affinity and number of kidney dopamine receptors between the CILAZA and the control groups. In conclusion, the diuretic effect caused by cilazapril is partly mediated by inhibition of the water reabsorption via the increase of dopamine production in the kidney.</p>
en-copyright= kn-copyright= en-aut-name=WatanabeHitoshi en-aut-sei=Watanabe en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OguraToshio en-aut-sei=Ogura en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HosoyaMasaharu en-aut-sei=Hosoya en-aut-mei=Masaharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishidaNorikazu en-aut-sei=Nishida en-aut-mei=Norikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=dopamine kn-keyword=dopamine en-keyword=ACE inhibitor kn-keyword=ACE inhibitor en-keyword=cilazapril kn-keyword=cilazapril en-keyword=SHR kn-keyword=SHR en-keyword=kidney kn-keyword=kidney END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=241 end-page=245 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Combined therapy with interleukin 2 and indomethacin in mice inoculated with MH134 hepatoma. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The antitumor effects of indomethacin and interleukin 2 (IL-2) were studied in C3H/HeJ mice inoculated with MH134 hepatoma cells. Combined treatment with indomethacin and IL-2 augmented natural killer (NK) cells in mice with MH134-induced peritoneal carcinomatosis, and the survival of the treated mice was significantly longer than the non-treated mice. In animals with subcutaneous MH134 tumors, the combined therapy with indomethacin and IL-2 significantly suppressed tumor growth and induced complete regression of the tumor in three out of five mice. These results suggest that indomethacin and IL-2 therapy could be effective on human gastrointestinal cancer cells as well.
en-copyright= kn-copyright= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkamotoYasuhisa en-aut-sei=Okamoto en-aut-mei=Yasuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=GotohKiyotoshi en-aut-sei=Gotoh en-aut-mei=Kiyotoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HizutaAkio en-aut-sei=Hizuta en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YunokiShigeru en-aut-sei=Yunoki en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=indomethacin kn-keyword=indomethacin en-keyword=interleukin-2 kn-keyword=interleukin-2 en-keyword=mouse kn-keyword=mouse en-keyword=hepatoma kn-keyword=hepatoma END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=237 end-page=240 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of pregnancy on plasma phenobarbital concentrations in rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We examined the pharmacokinetics of phenobarbital before and during pregnancy in rats. Animals were divided into four groups: (a) control, (b) pregnant, (c) phenobarbital-treated, and (d) phenobarbital-treated pregnant groups. The increase in body weight of nonpregnant or pregnant rats was not influenced by long-term phenobarbital treatment. Plasma phenobarbital concentrations during the period of long-term phenobarbital treatment with a fixed dosage by body weight were not significantly affected by pregnancy. Furthermore, pregnancy did not affect pharmacokinetic parameters of phenobarbital between 0.25 and 24h after administration. These results suggest that pregnancy does not influence on the pharmacokinetics of long-term phenobarbital treatment at a fixed dosage by body weight.
en-copyright= kn-copyright= en-aut-name=MoriyamaMasahiro en-aut-sei=Moriyama en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DomotoHaruyo en-aut-sei=Domoto en-aut-mei=Haruyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamashitaSyoichi en-aut-sei=Yamashita en-aut-mei=Syoichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FurunoKatsushi en-aut-sei=Furuno en-aut-mei=Katsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OishiRyozo en-aut-sei=Oishi en-aut-mei=Ryozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Kyushu University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=phenobarbital kn-keyword=phenobarbital en-keyword=pharmacokinetics kn-keyword=pharmacokinetics en-keyword=pregnancy kn-keyword=pregnancy en-keyword=plasma concentrations kn-keyword=plasma concentrations END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=267 end-page=270 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Relationship between body mass index (BMI) and changes in plasma total and HDL-cholesterol levels during treatment of hypertension in African patients. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Eighty-one adult Nigerians with essential hypertension were randomly allocated to receive doxazosin, hydrochlorothiazide/amloride, or amlodipine. In each group, the patients were further classified as obese and non-obese, and total cholesterol as well as high density lipoprotein (HDL) cholesterol was determined before and after the 3-month treatment period. The total cholesterol level was significantly reduced in the non-obese patients, but did not show any significant change in the obese patients after doxazosin therapy, indicating the beneficial effects of doxazosin therapy in non-obese patients. The levels of total cholesterol increased and HDL cholesterol decreased in both the obese and the non-obese patients after hydrochlorothiazide/amloride therapy. Amlodipine treatment did not cause any significant change in the total and HDL cholesterol levels in both the obese and non-obese patients. These findings are worthy of consideration by clinicians and researchers when selecting the most appropriate drug for antihypertensive pharmacotherapy.
en-copyright= kn-copyright= en-aut-name=AhanekuJoseph Eberendu en-aut-sei=Ahaneku en-aut-mei=Joseph Eberendu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AgbedanaOlu Emmanuel en-aut-sei=Agbedana en-aut-mei=Olu Emmanuel kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaylorOladunni Grace en-aut-sei=Taylor en-aut-mei=Oladunni Grace kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Nnamdi Azikiwe University, Nigeria affil-num=2 en-affil= kn-affil=University of Ibadan, Nigeria affil-num=3 en-affil= kn-affil=University of Ibadan, Nigeria en-keyword=body mass index kn-keyword=body mass index en-keyword=cholesterol kn-keyword=cholesterol en-keyword=hypertension kn-keyword=hypertension en-keyword=African patients kn-keyword=African patients END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=5 article-no= start-page=261 end-page=265 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Diagnostic sensitivity of motor nerve conduction studies in ulnar neuropathy at the elbow. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Seventy-six patients with ulnar neuropathy at the elbow were divided into 3 classes (Grades I, II, and III) according to their clinical features and the maximal motor nerve conduction velocity (MCV), and the amplitude ratios at the across-elbow segment were retrospectively analyzed. To determine the criteria for abnormality, a control study was conducted on 150 healthy volunteers ranging in age from 20 to 89 years (6 age groups). The normal value for MCV could be set for two age groups: those under 60 and those over 60 years old. The 95% confidence limit was 54m/s for the former and 50m/s for the latter. There was no statistically significant difference in the amplitude ratio among the age groups. The confidence limit was set uniformly at 0.82 (above elbow/below elbow). An abnormality in either MCV or the amplitude ratio was found in 66.7% of Grade I (recent and mild symptoms), 89.7% of Grade II (persistent symptoms), and 100% of Grade III cases (marked intrinsic muscle atrophy). Evaluation using the combination of MCV and the amplitude ratio, considering the age-related normal value, appeared to be useful in establishing a differential diagnosis of ulnar neuropathy at the elbow.
en-copyright= kn-copyright= en-aut-name=YokotaTadaaki en-aut-sei=Yokota en-aut-mei=Tadaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HamadaMasanori en-aut-sei=Hamada en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagashimaHiroaki en-aut-sei=Nagashima en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University Hospital en-keyword=entrapment neuropathy kn-keyword=entrapment neuropathy en-keyword=ulnar nerve kn-keyword=ulnar nerve en-keyword=electrodiagnosis kn-keyword=electrodiagnosis en-keyword=M-wave kn-keyword=M-wave END