ID | 30871 |
JaLCDOI | |
FullText URL | |
Author |
Ohta, Jun
Ubuka, Toshihiko
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Abstract | It has been assumed that the in vivo reduction of 3-mercaptopyruvate, an intermediate of cysteine metabolism, to 3-mercaptolactate is catalyzed by lactate dehydrogenase (EC 1.1.1.27) though no definitive evidence has been presented. In order to examine this assumption, reduction of 3-mercaptopyruvate and its inhibition were studied using rat liver homogenate, lactate dehydrogenase purified from rat liver and anti-lactate dehydrogenase antiserum. Reduction of 3-mercaptopyruvate was actively catalyzed by rat liver homogenate and by the purified lactate dehydrogenase. This reducing activity was completely inhibited by anti-lactate dehydrogenase antiserum. These results indicate that the reduction of 3-mercaptopyruvate to 3-mercaptolactate in rat liver is catalyzed by lactate dehydrogenase. |
Keywords | 3-mercaptopyruvate
3-mercaptolactate
lactate dehydrogenase
antiserum
cysteine metabolism
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 1989-04
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Volume | volume43
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Issue | issue2
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Publisher | Okayama University Medical School
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Start Page | 89
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End Page | 95
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |