JaLCDOI 10.18926/AMO/30875
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Author Kikkawa, Kiyoshi| Narahara, Kouji| Kimoto, Hiroshi|
Abstract <p>We performed a cytogenetic study on 140 nonpolymalformed patients with mental retardation of clinically undefined origin, using a high resolution banding technique, to determine how much chromosome abnormalities contribute to the etiology of this condition. A total of 15 patients (10.7%) were found to have autosomal or sex chromosomal abnormalities. Autosomal abnormalities included partial monosomy (5 cases), reciprocal translocation (one case), 13/14 robertsonian translocation (3 cases), unbalanced translocation (one case), inverted duplication of 15q (one case) and mosaic trisomy 21 (one case). Sex chromosomal abnormalities comprised structural rearrangement of the short arm of the X chromosome (one case) and 47, XXY in a pure or mosaic form (two cases). It should be noted that four out of the 5 cases of partial monosomy had subtle interstitial deletions, which might have been unidentified by the conventional G-banding method alone. In one case of the robertsonian translocation 46,XY,t(13;14)/45,XY,t(13;14), a small deletion was thought to have occurred in the cells with a chromosome number of 45. Comparison of clinical features of the 15 chromosomally abnormal patients with those of patients with normal karyotypes did not show any clinical parameter indicative of chromosome imbalance. These results suggest that a subtle chromosomal deletion is specific to mental retardation associated with few malformations. We believe that diagnostic evaluation of mentally retarded patients, even if nonmalformed, should include chromosome analysis using a high resolution banding technique.</p>
Keywords chromosomes high resolution banding technique subtle interstitial deletion mental retardation
Amo Type Article
Published Date 1989-04
Publication Title Acta Medica Okayama
Volume volume43
Issue issue2
Publisher Okayama University Medical School
Start Page 105
End Page 114
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2728904
Web of Sience KeyUT A1989U578500005
JaLCDOI 10.18926/AMO/30874
FullText URL fulltext.pdf
Author Sonobe, Hiroshi| Hayashi, Kazuhiko| Takahashi, Kiyoshi|
Abstract <p>A case of ovarian leiomyoma is reported, together with histologic, immunohistologic and electron microscopic findings. A solid firm tumor, measuring 6.5 X 5 X 5 cm, was found in the right ovary of a 65-year-old woman. The tumor had an obvious whorled pattern on the cut-surface. Well-differentiated, long spindle-shaped neoplastic cells revealed positive immunoreactivity for anti-desmin. Ultrastructural observations included numerous microfilaments with dense patches in the cytoplasm, micropinocytotic vesicles beneath plasma membranes and continuous basal laminae around neoplastic cells. These findings were compatible with leiomyoma. The possible histogenesis of ovarian leiomyoma was discussed.</p>
Keywords leiomyoma ovary immunohistochemistry ultrastructure
Amo Type Article
Published Date 1989-04
Publication Title Acta Medica Okayama
Volume volume43
Issue issue2
Publisher Okayama University Medical School
Start Page 131
End Page 134
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2728906
Web of Sience KeyUT A1989U578500008
JaLCDOI 10.18926/AMO/30873
FullText URL fulltext.pdf
Author Seki, Shuji| Arakaki, Yusei| Oda, Takuzo|
Abstract <p>A simple cell-free system for studying a priming factor involved in the repair of bleomycin-damaged DNA was established. The template-primer used for the repair DNA synthesis was prepared by treating the closed circular, superhelical form of pUC19 plasmid DNA with 2.2 microM bleomycin and 20 microM ferrous ions. Single-strand breaks were introduced into pUC19 DNA by the bleomycin treatment, and the DNA was consequently converted largely into the open circular form. A system for repair of this bleomycin-damaged DNA was constructed with a priming factor, DNA polymerase (DNA polymerase beta or Klenow fragment of DNA polymerase I), ATP, T4 DNA ligase and four deoxynucleoside triphosphates. After incubation, the conformation of the DNA was analyzed by agarose gel electrophoresis and electron microscopy. The open circular DNA was largely converted to the closed circular DNA, indicating that the single-strand breaks of DNA were repaired. When the priming factor was omitted, DNA repair did not occur. The present system seemed to be applicable to the study of priming factors involved in the repair of DNA with single-strand breaks caused not only by bleomycin but also by ionizing radiation or active oxygen.</p>
Keywords priming factor exonuclease DNA repair bleomycin pUC19 DNA agarosegel electrophoresis
Amo Type Article
Published Date 1989-04
Publication Title Acta Medica Okayama
Volume volume43
Issue issue2
Publisher Okayama University Medical School
Start Page 73
End Page 80
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2471391
Web of Sience KeyUT A1989U578500001
JaLCDOI 10.18926/AMO/30872
FullText URL fulltext.pdf
Author Ichikawa, Yoshiko|
Abstract <p>Murine adrenal tumor cells (Y-1 clone) were stimulated by adrenocorticotropic hormone (ACTH) and cyclic adenosine 3',5'-monophosphate (cyclic AMP) to produce steroid hormone (delta 4, 3-keto steroids). The steroids were secreted into the medium immediately after synthesis. The optimum concentrations of ACTH and cyclic AMP for stimulation of steroid production were 10(-2) U/ml and 1.0 mM, respectively. In serum-free medium, ACTH and cyclic AMP stimulated steroidogenesis in Y-1 cells, but the amount of steroid hormone in the culture medium was low. However, a high level of steroid production was maintained with medium containing 10 mg/ml bovine serum albumin (BSA). In culture medium containing a higher concentration of BSA, Y-1 cells did not become spherical as is usually the case when steroid production is stimulated by ACTH or cyclic AMP. The morphological changes did not always correlate with steroid secretion by Y-1 cells.</p>
Keywords Y-1 clone steroid hormone ACTH cyclic AMP
Amo Type Article
Published Date 1989-04
Publication Title Acta Medica Okayama
Volume volume43
Issue issue2
Publisher Okayama University Medical School
Start Page 97
End Page 103
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2543184
Web of Sience KeyUT A1989U578500004
JaLCDOI 10.18926/AMO/30871
FullText URL fulltext.pdf
Author Ohta, Jun| Ubuka, Toshihiko|
Abstract <p>It has been assumed that the in vivo reduction of 3-mercaptopyruvate, an intermediate of cysteine metabolism, to 3-mercaptolactate is catalyzed by lactate dehydrogenase (EC 1.1.1.27) though no definitive evidence has been presented. In order to examine this assumption, reduction of 3-mercaptopyruvate and its inhibition were studied using rat liver homogenate, lactate dehydrogenase purified from rat liver and anti-lactate dehydrogenase antiserum. Reduction of 3-mercaptopyruvate was actively catalyzed by rat liver homogenate and by the purified lactate dehydrogenase. This reducing activity was completely inhibited by anti-lactate dehydrogenase antiserum. These results indicate that the reduction of 3-mercaptopyruvate to 3-mercaptolactate in rat liver is catalyzed by lactate dehydrogenase.</p>
Keywords 3-mercaptopyruvate 3-mercaptolactate lactate dehydrogenase antiserum cysteine metabolism
Amo Type Article
Published Date 1989-04
Publication Title Acta Medica Okayama
Volume volume43
Issue issue2
Publisher Okayama University Medical School
Start Page 89
End Page 95
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2499162
Web of Sience KeyUT A1989U578500003
JaLCDOI 10.18926/AMO/30870
FullText URL fulltext.pdf
Author Mori, Shigeru| Seki, Shuji| Oda, Takuzo|
Abstract <p>To study the mechanism of DNA excision repair, a DNA repair system employing permeable mouse sarcoma (SR-C3H/He) cells was established and characterized. SR-C3H/He cells were permeabilized with a 0.0175% Triton X-100 solution. The permeable cells were treated with 1 mM ATP and 0.11 mM bleomycin, and then washed thoroughly to remove ATP and bleomycin. Repair DNA synthesis occurred in the bleomycin-damaged, permeable SR-C3H/He cells when incubated with ATP and four deoxyribonucleoside triphosphates. The repair nature of the DNA synthesis was confirmed by the BrdUMP density shift technique, and by the reduced sensitivity of the newly synthesized DNA to Escherichia coli exonuclease III. The DNA synthesis was optimally enhanced by addition of 0.08 M NaCl. Studies using selective inhibitors of DNA synthesis showed that aphidicolin-sensitive DNA polymerase (DNA polymerase alpha and/or delta) and DNA polymerase beta were involved in the repair process. The present DNA repair system is thought to be useful to study nuclear DNA damage by bleomycin, removal of the damaged ends by an exonuclease, repair DNA synthesis by DNA polymerases and repair patch ligation by DNA ligase(s).</p>
Keywords DNA repair bleomycin DNA polymerases permeable cells mouse ascites cells
Amo Type Article
Published Date 1989-04
Publication Title Acta Medica Okayama
Volume volume43
Issue issue2
Publisher Okayama University Medical School
Start Page 81
End Page 88
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2471392
Web of Science KeyUT A1989U578500002
JaLCDOI 10.18926/AMO/30869
FullText URL fulltext.pdf
Author Ikeda, Shogo| Tsutsui, Ken| Hatsushika, Masao| Watanabe, Sekiko| Oda, Takuzo|
Abstract <p>The major gag protein (p34) of squirrel monkey retrovirus-H was purified in one chromatographic step by anion-exchange high performance liquid chromatography. The virus in a crude fraction was disrupted with Brij 35 in the presence of three kinds of protease inhibitors. The soluble virus lysate was injected into a Polyanion SI column, and p34 was eluted with a linear salt gradient. The recovery of the protein was about 60%. The purified p34 was nearly homogenous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining.</p>
Keywords retrovirus gag protein protein purification high performance liquid chromatography
Amo Type Article
Published Date 1989-04
Publication Title Acta Medica Okayama
Volume volume43
Issue issue2
Publisher Okayama University Medical School
Start Page 127
End Page 129
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2786318
Web of Sience KeyUT A1989U578500007
JaLCDOI 10.18926/AMO/30868
FullText URL fulltext.pdf
Author Miyashima, Takanao| Hayashi, Keiki| Awai, Michiyasu|
Abstract <p>To clarify the initiation, development and recovery processes of disseminated intravascular coagulation (DIC), rat glomerular capillaries and fibrin thrombi were examined under transmission and scanning electron microscopes. DIC was induced in rats by a single intraperitoneal injection of endotoxin (Et., 7.5 mg/kg lipopolysaccharide:B, E. coli 026:B6). At 2 h after Et. injection, the endothelial surface of the glomerular capillary became irregular with projections like a sea anemone. At 4 h after Et. injection, agglomerated fibrin thrombi composed of fibrin fiber bundles with fine cross-striated fibriform structures were observed in the capillary lumen. The fibrin thrombi gradually changed into fine reticular systems suggesting a degradation process by 6 h after Et. injection, and formed a coarse granular agglomerate by 8 h after Et. injection. These fibrin thrombi disappeared within 12 h of Et. injection, but the endothelial surface remained edematous. At 24 h after Et. injection, the microstructure of the glomerular capillaries returned normal. Based on these observations, we concluded that DIC was primarily initiated by injury to the capillary endothelium, and that changes on the endothelial surface contributed to the development of DIC.</p>
Keywords disseminated intravascular coagulation renal tissue electron microscope rat
Amo Type Article
Published Date 1989-04
Publication Title Acta Medica Okayama
Volume volume43
Issue issue2
Publisher Okayama University Medical School
Start Page 115
End Page 126
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2728905
Web of Science KeyUT A1989U578500006