JaLCDOI 10.18926/AMO/30899
FullText URL fulltext.pdf
Author Okita, Misako| Watanabe, Akihara| Tsuji, Takao|
Abstract <p>The plasma fatty acid composition of cirrhotic patients and their dietary intake of fatty acids were determined. Significantly lower plasma arachidonic, docosahexaenoic, dihomo-gamma-linolenic and eicosapentaenoic acid levels were observed in cirrhotic patients than in healthy controls. A remarkably low dietary intake of polyunsaturated fatty acids supplied from fish, vegetable oil and pulses was shown in cirrhotic patients. Positive correlations were observed between plasma arachidonic acid concentrations and clearance rate of indocyanine green (KICG) (r = 0.826, p less than 0.05) and between dihomo-gamma-linolenic acid levels and cholinesterase activities (r = 0.841, p less than 0.05). From these results, we conclude that a supply of polyunsaturated fatty acids is necessary for the nutritional treatment of patients with liver cirrhosis.</p>
Keywords lipid malnutrition liver cirrhosis polyunsaturated fatty acid
Amo Type Article
Published Date 1989-02
Publication Title Acta Medica Okayama
Volume volume43
Issue issue1
Publisher Okayama University Medical School
Start Page 39
End Page 45
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2718770
Web of Sience KeyUT A1989T938500005
JaLCDOI 10.18926/AMO/30898
FullText URL fulltext.pdf
Author Nawa, Sugato| Yamada, Makoto| Sugawara, Eiji| Kino, Kohichi| Teramoto, Shigeru|
Abstract <p>Geometrical measurements of angiocardiograms of the common outflow tract (COT) of 13 patients were made to determine in which cases internal conduit repair was feasible, and under which conditions a patch enlargement of the COT was indicated. In the pulmonary stenosis (PS) group, the area of the narrowest cross-section of the COT was significantly smaller than that in the pulmonary hypertension (PH) group (p less than 0.025). In the PS group, the area was rarely sufficient to be shared by systemic and pulmonary circulation. Therefore, stenosis in the outflow tract to the pulmonary artery will occur if the intraventricular tunnel technique is applied, without patch enlargement of this portion, to patients with PS. On the contrary, the cross-sectional areas of the COT and pulmonary arteries were significantly larger in the PH group than in the PS group. Accordingly, the intracardiac conduit operation may be possible in such patients without a patch enlargement, even in young patients if other intracardiac conditions allow. Preoperative angiocardiographic evaluation of the COT is helpful in preoperatively selecting the proper operative procedure for this anomaly.</p>
Keywords double-outlet right ventricle angiocardiography common outflow tract intraventricular tunnel technique patch enlargement
Amo Type Article
Published Date 1989-02
Publication Title Acta Medica Okayama
Volume volume43
Issue issue1
Publisher Okayama University Medical School
Start Page 65
End Page 72
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2718771
Web of Sience KeyUT A1989T938500008
JaLCDOI 10.18926/AMO/30897
FullText URL fulltext.pdf
Author Ikeda, Takahiro| Ubaka, Toshihiko| Ishino, Kazushi|
Abstract <p>The isoelectric point (pI) value of 3-mercaptopyruvate sulfurtransferase (MST) from human erythrocytes was determined to be 6.3 at 10 degrees C by isoelectric focusing in horizontal slab polyacrylamide gel containing 2% carrier ampholyte (pH 3-10). The value was determined by comparison with the electrofocused bands of bovine pancreatic ribonuclease A-glutathione mixed disulfides (RNase-SG), which were composed of 8 species containing 1 (RNase-SG1) through 8 (RNase-SG8) moles of glutathione per mole of ribonuclease A with different pI values ranging from 5.3 (RNase-SG8) to 8.8 (RNase-SG1). The pI value of the same enzyme in a 110,000 X g supernatant of rat liver was 5.9, which was the same as that of rat erythrocyte enzyme. Treatments of rat hemolysate with oxidized glutathione or diamide resulted in a shift of the pI of MST to a lower value, 5.7-5.5. This shift was inhibited when these treatments were performed in the presence of dithiothreitol. These results indicate that the treatment of the enzyme with oxidized glutathione results in the formation of enzyme-glutathione mixed disulfide.</p>
Keywords 3-mercaptopyruvate sulfurtransferase isoelectric point glutathione mixed disulfide isoelectric focusing
Amo Type Article
Published Date 1989-02
Publication Title Acta Medica Okayama
Volume volume43
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 7
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2718769
Web of Sience KeyUT A1989T938500001
JaLCDOI 10.18926/AMO/30896
FullText URL fulltext.pdf
Author Hayashi, Keiki| Hsueh, Chao-Liang| Kawasaki, Hideaki| Toyofuku, Hiroyuki| Miyashima, Takanao| Awai, Michiyasu|
Abstract <p>Renal tissues from 208 human necropsies were observed histologically for disseminated intravascular coagulation (DIC). The tissues were stained with hematoxylin-eosin, Mallory's phosphotungstic acid hematoxylin (PTAH) and cationic ferric hydroxide colloid stabilized with cacodylate (Fe-Cac), and tested by immunoenzyme histochemical (IEH) reaction for fibrin-related materials (FRMs). The use of the IEH method increased FRM recognition, and FRMs were detected in a total of 80 cases (38.5%). In 26 cases diagnosed clinically as DIC, FRMs were shown in 23 of the cases (88.5%). Thus, 57 patients with FRMs were clinically asymptomatic. In rats with DIC induced by endotoxin injection, glomerulus FRM was effluxed into the tubulus through the Bowman's capsule and was excreted into urine. The electric charge was reduced on the endothelial surface of the glomerular capillaries in both human and rat DIC. Under the scanning electron microscopy, the endothelial surface appeared coarse in the glomerular capillary and fibrin degradation was present. Our conclusions are: (a) PTAH is non-specific for FRMs, (b) IEH aids the pathohistological diagnosis of DIC, especially in asymptomatic forms including the compensated DIC state, (c) FRMs in tubuli suggest DIC, and (d) DIC is possibly initiated by a reduction in the capillary electric surface charge.</p>
Keywords disseminated intravascular coagulation immunoenzyme-histochemistry fibrinrelated materials
Amo Type Article
Published Date 1989-02
Publication Title Acta Medica Okayama
Volume volume43
Issue issue1
Publisher Okayama University Medical School
Start Page 29
End Page 38
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2470234
Web of Sience KeyUT A1989T938500004
JaLCDOI 10.18926/AMO/30895
FullText URL fulltext.pdf
Author Nishiyama, Yoshitaka| Fuchimoto, Sadanori| Orita, Kunzo|
Abstract <p>We investigated the antitumor effect of purified natural human tumor necrosis factor-beta (nHuTNF-beta) produced by human acute lymphoblastic leukemia BALL-1 cells stimulated with HVJ on pulmonary metastatic tumors of Lewis lung carcinoma (3LL) transplanted into BDF1 mice. nHuTNF-beta showed antiproliferative effects on metastatic tumors in a dose-dependent manner when administered daily for 10 days by the intravenous route. Histological examination of the tumors treated with nHuTNF-beta revealed that the tumor size and number of metastases were much reduced. Lytic cellular changes, including cytoplasmic vacuolation, loosening of the intercellular junction and both cytoplasmic and nuclear swelling, were found, but tumor necrosis was not. These findings indicate a therapeutic effect of Grade IIa according to the histological criteria of Shimosato and Ohboshi. In addition, synergistic augmentation of the antiproliferative effects of nHuTNF-beta by natural murine interferon-alpha/beta (nMu-IFN-alpha/beta) or recombinant murine interferon-gamma (rMuIFN-gamma) was recognized by median effect plot analysis. The results suggested that nHuTNF-beta may well deserve clinical trial as a new immunotherapeutical agent for human cancer.</p>
Keywords tumor necrosis factor interferon Lewis lung carcinoma synergistic potentiation antitumor effect
Amo Type Article
Published Date 1989-02
Publication Title Acta Medica Okayama
Volume volume43
Issue issue1
Publisher Okayama University Medical School
Start Page 17
End Page 27
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2470233
Web of Sience KeyUT A1989T938500003
JaLCDOI 10.18926/AMO/30894
FullText URL fulltext.pdf
Author Ohsawa, Toshiya| Higashi, Toshihiro| Tsuji, Takao|
Abstract <p>The biochemical characteristics of cathepsin B secreted from cultured human liver cancer cells were examined. The enzyme activity of culture medium against a synthetic substrate, N-carbobenzoxy-L-arginyl-L-arginine-4-methyl-coumaryl-7-amide, was dependent on the addition of cysteine, and the optimal pH was found to be 6.0. No activity was observed when the enzyme source was fresh medium not used for culture. These results suggest that the enzyme released from liver cancer cells is the thiol-protease cathepsin B. The molecular weight of the enzyme with 90% of the total activity was 40,000. Two cathepsin B molecules were found in liver tissue from patients with hepatocellular carcinoma (HCC); one was equivalent in size to the secreted enzyme, and a smaller one was the same as normal liver cathepsin B (27,000), which was also obtained from HCC-bearing cirrhotic liver. These results demonstrate that two molecules of cathepsin B are synthesized in liver cancer, and that the larger one is released into the surrounding tissue.</p>
Keywords cathepsin B cathepsin B secretion cultured human liver cancer
Amo Type Article
Published Date 1989-02
Publication Title Acta Medica Okayama
Volume volume43
Issue issue1
Publisher Okayama University Medical School
Start Page 9
End Page 15
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2718772
Web of Sience KeyUT A1989T938500002
JaLCDOI 10.18926/AMO/30893
FullText URL fulltext.pdf
Author Kusachi, Shozo| Iwasaki, Kohichirou| Nishiyama, Osamu| Ueda, Minoru| Kita, Toshimasa| Hata, Takato| Taniguchi, Gyou| Watanabe, Hirofumi| Hina, Kazuyoshi| Saito, Daiji| Tsuji, Takao| Haraoka, Shoichi|
Abstract <p>We studied the factors which may induce acute high grade restenosis in emergency percutaneous transluminal coronary angioplasty (PTCA). PTCA was attempted in 50 patients with acute myocardial infarction, and the balloon catheter passed successfully across the occlusion site in 47 (94%) of the patients. These 47 patients were analyzed. &#34;Acute restenosis&#34; was defined as a lesion which was revascularized to less than 50% luminal reduction narrowed again to more than 75% luminal reduction 5 min after the balloon inflation. Univariate and multivariate analyses were used for determining factors which significantly influenced acute restenosis. The incidence of at least one restenosis episode was 45%. Multiple regression analysis selected 5 factors associated significantly with an increased rate of acute restenosis: 1) angiographic evidence of dissection, 2) lesion in the right coronary artery (RCA), 3) lack of or insufficient administration of thrombolytic agent preceding PTCA, 4) curved lesion and 5) relatively small balloon/artery diameter ratio. Acute restenosis correlated significantly with late reocclusion. This study indicates that it is important to administer a thrombolytic agent prior to emergency PTCA, and to use an adequately sized balloon to the artery when the acute restenosis occurs by using relatively smaller sized balloon. The present data also demonstrated that patients with RCA and a curved lesion have a relatively high risk of acute restenosis. This study indicates how patients with relatively high risk of acute restenosis may be identified.</p>
Keywords emergency coronary angioplasty restenosis acute myocardial infarction multivariate analysis
Amo Type Article
Published Date 1989-02
Publication Title Acta Medica Okayama
Volume volume43
Issue issue1
Publisher Okayama University Medical School
Start Page 55
End Page 63
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2524153
Web of Sience KeyUT A1989T938500007
JaLCDOI 10.18926/AMO/30892
FullText URL fulltext.pdf
Author Sunakawa, Mitsuru| Kohmoto, Tomoji| Komoto, Yoshiaki|
Abstract <p>Improvement in tissue perfusion following surgically induced ischemia in limbs of dogs was experimentally evaluated to clarify the improvement of hemodynamics following walking exercise in chronic, peripheral arterial occlusive diseases. With the use of a computer system in conjunction with medical mass spectrometry, the local tissue perfusion rate was calculated on the basis of the clearance curve of tissue partial pressure of CO2 following electrical stimulation of the ischemic leg to simulate exercise. Ischemia was created in the leg by ligation of the proximal and peripheral arteries. In one month, intermittent claudication improved in accordance with improvement in muscle tissue perfusion. Angiographic evidence of distal runoff became visible six months after surgery, indicating that tissue perfusion played an important role in peripheral hemodynamics. The local tissue perfusion rate improved from 9.51 +/- 2.62 ml/100 g/min to 12.41 +/- 2.42 in one month, to 14.59 +/- 3.19 in three months, to 15.11 +/- 3.24 in six months and to 17.19 +/- 2.63 in twelve months. The improvement of ischemic symptoms following long-term exercise is attributed to improvements in tissue perfusion or collateral circulation.</p>
Keywords ischemic legs tissue perfusion mass spectrometry walking exercise intermittent claudication
Amo Type Article
Published Date 1989-02
Publication Title Acta Medica Okayama
Volume volume43
Issue issue1
Publisher Okayama University Medical School
Start Page 47
End Page 54
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2497622
Web of Sience KeyUT A1989T938500006