Cholestasis with htperbilirubinemia was induced in female, but not male, Sprague-Dawley rats by daily treatment with phalloidin for 7 days. Increases in serum direct bilirubin level and alkaline phosphatase (Al-Pase) activity were observed concomitantpy with diminished bile flow and a decreased output of bile acid and cholesterol. Kidht microscope findings of the liver revealed proliferated bile ductules and enhanced mitosis of hepatocytes.
en-copyright= kn-copyright= en-aut-name=ObataTakahiro en-aut-sei=Obata en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HayashiShosuku en-aut-sei=Hayashi en-aut-mei=Shosuku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakeiNobuyuki en-aut-sei=Takei en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SakataTatsuro en-aut-sei=Sakata en-aut-mei=Tatsuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HigashiToshihiro en-aut-sei=Higashi en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ItoshimaTatsuya en-aut-sei=Itoshima en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University en-keyword=phalloidin kn-keyword=phalloidin en-keyword=cholestasis kn-keyword=cholestasis en-keyword=hyperbilirubinemia kn-keyword=hyperbilirubinemia en-keyword=mitosis kn-keyword=mitosis en-keyword= bile ductule. kn-keyword= bile ductule. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=6 article-no= start-page=427 end-page=430 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A simple method for purification of rat alpha-fetoprotein by affi-gel blue chromatography and disc electrophoresis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Alpha-getoprotein (AFP) was purified from fetal rat serum by a combined technique of affinity chromatography with Affi-Gel Blue and disc electrophoresis followed by extraction of AFP from the gel. The purified AFP was immunologically identical with the original AFP in fetal rat serum.
en-copyright= kn-copyright= en-aut-name=MiyazakiMasahiro en-aut-sei=Miyazaki en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsuuraKazuhiko en-aut-sei=Matsuura en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WahidSyarifuddin en-aut-sei=Wahid en-aut-mei=Syarifuddin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IzumiMasaki en-aut-sei=Izumi en-aut-mei=Masaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TaketaKazuhisa en-aut-sei=Taketa en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SatoJiro en-aut-sei=Sato en-aut-mei=Jiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Hasanuddin University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Health Research Center, Kagawa University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=purification of rat AFP kn-keyword=purification of rat AFP en-keyword=fetal rat serum kn-keyword=fetal rat serum en-keyword=affinity chromatography kn-keyword=affinity chromatography en-keyword= disc electrophoresis. kn-keyword= disc electrophoresis. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=6 article-no= start-page=385 end-page=394 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Quantitative determination of urinary metabolites in subjects exposed to organic solvents. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Most of the common organic solvents are excreted into urine as metabolites. A correlation exists for several organic solvents between the amount taken in and the amount of metabolites excreted. Many methods have been developed for the measurement of these urinary metabolites. The methods were classified into three group: colorimetry, gas chromatography and high performance liquid chromatography. The characteristics and availability of these methods in a laboratory for routine work were reviewed. The correction equation for the amounts of metabolites in spot urine is discussed.
en-copyright= kn-copyright= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=organic solvents kn-keyword=organic solvents en-keyword=urinary metabolites kn-keyword=urinary metabolites en-keyword=colorimetry kn-keyword=colorimetry en-keyword=gaschromatography kn-keyword=gaschromatography en-keyword= high performance liquid chromatography. kn-keyword= high performance liquid chromatography. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=6 article-no= start-page=411 end-page=415 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Hyperbilirubinemia and enhanced mitosis of hepatocytes in phalloidin-treated female rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cholestasis with hyperbilirubinemia was induced in female, but not male, Sprague-Dawley rats by daily treatment with phalloidin for 7 days. Increases in serum direct bilirubin level and alkaline phosphatase (Al-Pase) activity were observed concomitantly with diminished bile flow and a decreased output of bile acid and cholesterol. Light microscope findings of the liver revealed proliferated bile ductules and enhanced mitosis of hepatocytes.
en-copyright= kn-copyright= en-aut-name=ObataTakahiro en-aut-sei=Obata en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HayashiShosaku en-aut-sei=Hayashi en-aut-mei=Shosaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakeiNobuyuki en-aut-sei=Takei en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SakataTatsuro en-aut-sei=Sakata en-aut-mei=Tatsuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HigashiToshihiro en-aut-sei=Higashi en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ItoshimaTatsuya en-aut-sei=Itoshima en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University en-keyword=phalloidin kn-keyword=phalloidin en-keyword=cholestasis kn-keyword=cholestasis en-keyword=hyperbilirubinemia kn-keyword=hyperbilirubinemia en-keyword=mitosis kn-keyword=mitosis en-keyword= bile ductule. kn-keyword= bile ductule. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=6 article-no= start-page=407 end-page=410 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Detection of hepatitis B surface antigen subtype adr in an epidemic of papular acrodermatitis of childhood (Gianotti's disease). en-subtitle= kn-subtitle= en-abstract= kn-abstract=Papular acrodermatitis of childhood (PAC) has recently been reported to be associated with hepatitis B surface antigen (HBsAg) subtype ayw. Between September, 1978, and June, 1979, we saw 14 patients with PAC in a small epidemic occurring in Iwakuni City, Japan. HBsAg was detected in sera from all patients. Subtyping of HBsAg in 11 patients showed that 8 had a determinant adr and 3 had no detectable determinant because of low antigen titers. The result suggests that factors other than the specific HBsAg subtype contribute to the development of PAC.
en-copyright= kn-copyright= en-aut-name=KanzakiSusumu en-aut-sei=Kanzaki en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KanddaSoichiro en-aut-sei=Kandda en-aut-mei=Soichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TeradaKuniko en-aut-sei=Terada en-aut-mei=Kuniko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NohnoShin en-aut-sei=Nohno en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KumanoKeiichi en-aut-sei=Kumano en-aut-mei=Keiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NaraharaKouji en-aut-sei=Narahara en-aut-mei=Kouji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HayashiHiromitsu en-aut-sei=Hayashi en-aut-mei=Hiromitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KimotoHiroshi en-aut-sei=Kimoto en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Iwakuni National Hospital affil-num=3 en-affil= kn-affil=Iwakuni National Hospital affil-num=4 en-affil= kn-affil=Iwakuni National Hospital affil-num=5 en-affil= kn-affil=Kumano Pediatric Clinic affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=papilar acrodermatitis of childhood kn-keyword=papilar acrodermatitis of childhood en-keyword=Gianotti's disease kn-keyword=Gianotti's disease en-keyword=hepatitis B virus kn-keyword=hepatitis B virus en-keyword= hepatitis B surface antigen subtype. kn-keyword= hepatitis B surface antigen subtype. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=6 article-no= start-page=431 end-page=433 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Change in somatosensory evoked potential in the rat recorded at the hemisphere with iron-induced epileptic focus. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In rats, microinjection of FeCl3 solution into the left sensorimotor cortex was performed to induce a chronic epileptic focus. One month or more after the microinjection, electrocutaneous stimuli were applied to part of the wrist joint and 50 consecutive somatosensory evoked potentials (SEPs) were averaged. SEP from the left cortex showed only an initial negative monophasic deflection while SEP from the contralateral cortex showed a normal configuration with initial positive-negative biphasic deflection in the majority of experimental animals.
en-copyright= kn-copyright= en-aut-name=HattoriYukio en-aut-sei=Hattori en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UezuEiko en-aut-sei=Uezu en-aut-mei=Eiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MoriwakiAkiyoshi en-aut-sei=Moriwaki en-aut-mei=Akiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HoriYasuo en-aut-sei=Hori en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=somatosensory EVP kn-keyword=somatosensory EVP en-keyword=iron-induced focus kn-keyword=iron-induced focus en-keyword=rat. kn-keyword=rat. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=6 article-no= start-page=395 end-page=405 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Tissue distribution and antitumor effect of liposome-entrapped doxorubicin (adriamycin) in Ehrlich solid tumor-bearing mouse. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The usefulness of liposomes (in neutral, positively and negatively charged forms) as a carrier for adriamycin (ADM) was studied by examining the distribution of ADM and related fluorescent compounds in Ehrlich solid tumor-bearing mice. The mice were given free or liposome-entrapped ADM intraperitoneally. The distribution of ADM and related fluorescent compounds between the administration of the free form and liposome-entrapped form was measured by high performance liquid chromatography : The distribution was dependent on the form of the liposomes. The amounts of ADM and its metabolites in the mouse serum 20 min after administration of neutral-liposome-entrapped ADM were 10 times those after the administration of free ADM, 6 times those after the administration of a negatively charged form, and 3.5 times those in the administration of positively charged form. There was no marked difference in the concentrations of these compounds 5 h after administration. The concentration of these compounds in the liver 60 min after administration of each liposome-entrapped form of ADM were in inverse correlation with the concentrations in the serum obtained at 20 min after administration. Total concentrations of ADM and its metabolites in the tumors 20 min after administration of each entrapped form of ADM were 4-5 times that in administration of free ADM after 20 min. There were no marked differences in the concentration of ADM for administration of the various liposome forms. Statistically significant decreases in mean tumor weight were seen in the groups given neutral, positively and negatively charged liposome-entrapped forms compared to corresponding control groups given with free ADM.
en-copyright= kn-copyright= en-aut-name=ShinozawaShinya en-aut-sei=Shinozawa en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ArakiYasunori en-aut-sei=Araki en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=adriamycin kn-keyword=adriamycin en-keyword=charged liposomes kn-keyword=charged liposomes en-keyword=tissue distribution kn-keyword=tissue distribution en-keyword=antitumor effect kn-keyword=antitumor effect en-keyword= high-performance liquid chromatography (HPLC). kn-keyword= high-performance liquid chromatography (HPLC). END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=6 article-no= start-page=421 end-page=425 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A case of supernumerary cuspid. en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KobayashiSeishi en-aut-sei=Kobayashi en-aut-mei=Seishi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishijimaKatsumi en-aut-sei=Nishijima en-aut-mei=Katsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YaoHisatake en-aut-sei=Yao en-aut-mei=Hisatake kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SugiHideki en-aut-sei=Sugi en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakayaYasuo en-aut-sei=Takaya en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IkedaTsuyoshi en-aut-sei=Ikeda en-aut-mei=Tsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University Dental School affil-num=2 en-affil= kn-affil=Okayama University Dental School affil-num=3 en-affil= kn-affil=Okayama University Dental School affil-num=4 en-affil= kn-affil=Okayama University Dental School affil-num=5 en-affil= kn-affil=Okayama University Dental School affil-num=6 en-affil= kn-affil=Ikeda Dental Office en-keyword=oral anatomy kn-keyword=oral anatomy en-keyword=supernumerary cuspid kn-keyword=supernumerary cuspid END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=6 article-no= start-page=417 end-page=420 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of motilin on the sphincter of Oddi in the dog. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To investigate the action of motilin on the sphincter of Oddi, the flow rate of the perfusate (FRP) discharged into the duodenal lumen through the orifice of the common bile duct was measured by means of an electric drop counter in decerebrated dogs. Motilin in doses above 0.5 micrograms/kg i.v. reduced or stopped the FRP. The fifty percent recovery time of FRP was 20 min and full recovery time was 30 min. The reduction of FRP induced by motilin was unaffected by denervation and atropinization. These results suggest that motilin caused an increase in tone of the sphincter of Oddi by acting on the sphincter muscle.
en-copyright= kn-copyright= en-aut-name=NeyaToshiaki en-aut-sei=Neya en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MizutaniMasatoshi en-aut-sei=Mizutani en-aut-mei=Masatoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakakiMiyako en-aut-sei=Takaki en-aut-mei=Miyako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakayamaSosogu en-aut-sei=Nakayama en-aut-mei=Sosogu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=bile duct kn-keyword=bile duct en-keyword=bile excretion kn-keyword=bile excretion en-keyword=choledocus kn-keyword=choledocus en-keyword=motilin kn-keyword=motilin en-keyword= sphincter of Oddi. kn-keyword= sphincter of Oddi. END