JaLCDOI 10.18926/AMO/31255
FullText URL fulltext.pdf
Author Okada, Yoshio| Arima, Terukatsu| Okazaki, Satoru| Nakata, Ken-ichi| Yamabuki, Takahiro| Nagashima, Hideo|
Abstract <p>Insulin binding to erythrocytes was studied in diabetic patients. Insulin binding was lower in untreated diabetics and diabetic patients treated with diet or insulin than in normal subjects. Binding variation was mainly due to decreased binding site concentration in untreated and insulin-treated patients, and to lowered insulin binding site affinity in diet-treated patients. Several patients treated with hypoglycemic agents showed higher insulin binding due to increased binding site concentration. Insulin binding to erythrocytes may not always reflect the insulin binding status of insulin sensitive tissues.</p>
Keywords insulin binding diabetes mellitus erythrocyte.
Amo Type Brief Note
Published Date 1981-10
Publication Title Acta Medica Okayama
Volume volume35
Issue issue4
Publisher Okayama University Medical School
Start Page 273
End Page 277
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457514
Web of Science KeyUT A1981MK84200005
JaLCDOI 10.18926/AMO/31254
FullText URL fulltext.pdf
Author Hattori, Yukiko| Inaba, Kozo| Hori, Yasuo|
Abstract <p>The effect of L-glutamate of cyclic AMP levels in different areas of rat cerebral cortex was examined during the development of an iron-induced focus in the left sensorimotor cortex. The addition of L-glutamate resulted in increased cyclic AMP levels in slices from the quarter of cortex with the iron-induced focus. The levels lowered in slices from the quarter of cortex most remote from the focus. Thus L-glutamate showed stimulatory and inhibitory effects on the accumulation of cyclic AMP.</p>
Keywords rat cerebral cortex cyclic AMP <sub>L</sub>-glutamate iron-induced focus.
Amo Type Brief Note
Published Date 1981-10
Publication Title Acta Medica Okayama
Volume volume35
Issue issue4
Publisher Okayama University Medical School
Start Page 289
End Page 291
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6117191
Web of Science KeyUT A1981MK84200008
JaLCDOI 10.18926/AMO/31253
FullText URL fulltext.pdf
Author Nakamoto, Shu|
Abstract <p>In order to get precise information about the movement of plasma membrane proteins in cap formation, cyto- and bio-chemical analyses were made of the plasma membranes from non-capped areas of Ehrlich ascites tumor cells (EATCs) exposed to concanavalin A (Con A). Blebs formed by treatment with cytochalasin B (CB) of the non-capped areas of cells having a cap were isolated and used as the plasma membranes from non-capped areas (ConA-CB-bleb fraction). This bleb fraction was compared with a bleb fraction prepared from cells without ConA-treatment (CB-bleb fraction). Cytochemical analysis of ConA-CB-bleb fraction revealed a decreased in conA binding sites (ConA-BS) compared to the CB-bleb fraction. SDS polyacrylamide slab gel electrophoresis also revealed a decrease in the major components of ConA-BS of the ConA-CB-bleb fraction. The minor components of ConA-BS showed no distinct quantitative difference between the ConA-CB-bleb and CB-bleb fractions. NA+ K+-adenosine triphosphatase (ATPase), 5' nucleotidase (5'ND) and gamma-glutamyl transpeptidase (gamma-GTP) did not show any decrease in activity in the ConA-CB-bleb fraction, but the activity of D+-stimulated phosphatase (K-Pase) was decreased. The findings indicate that there are two types of plasma membrane glycoproteins in EATCs; one includes those participating in cap formation due to ConA, e.g. the major components of ConA-BS and K-Pase, and the other, those not participating in such cap formation, e.g. some minor components of ConA-BS, ATPase, 5'ND and gamma-GTP, which keep their places without moving.</p>
Keywords concanavalin A cytochalasin B capping bleb biochemical analysis.
Amo Type Article
Published Date 1981-10
Publication Title Acta Medica Okayama
Volume volume35
Issue issue4
Publisher Okayama University Medical School
Start Page 221
End Page 234
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6117190
Web of Science KeyUT A1981MK84200001
JaLCDOI 10.18926/AMO/31252
FullText URL fulltext.pdf
Author Irino, Shoxo| Sanada, Hiroshi| Maesako, Naohisa| Tanaka, Toshio|
Abstract <p>A 63-year-old man developed generalized lymphadenopathy with skin rashes, fever, hepatomegaly and polyclonal hypergammaglobulinemia, twice, in February 1972 and in June 1979, after taking allopurinol for gout. Cervical lymph node biopsy, performed each time, showed the presence of immunoblasts and plasma cells, effaced nodal structure with involvement of the pericapsular tissue, rich vascularity and numerous mitoses, indicative of angio-immunoblastic lymphadenopathy with dysproteinemia (Frizzera, Moran and Rappaport). The existence of hypersensitivity to drugs, in particular, allopurinol in certain patients was emphasized, and induction of immunoblastic lymphadenopathy with various other therapeutic agents was briefly discussed.</p>
Keywords angio-immunoblastic lymphadenopathy allopurinol.
Amo Type Article
Published Date 1981-10
Publication Title Acta Medica Okayama
Volume volume35
Issue issue4
Publisher Okayama University Medical School
Start Page 263
End Page 272
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457513
Web of Science KeyUT A1981MK84200004
JaLCDOI 10.18926/AMO/31251
FullText URL fulltext.pdf
Author Hizuta, Akio|
Abstract <p>In order to investigate the immunological responsiveness of tumor-bearing hosts to tumor cells, splenic suppressor cells from Ehrlich tumor-bearing mice that inhibited anti-tumor effector cell activity were characterized. In vitro cell-mediated cytoxicity and cytostasis assays were performed to test for the existence of anti-tumor immunity. suppressive activity assayed by cell mixture experiments became apparent with decline of anti-tumor immunity and progressive tumor growth. The cells mediating the suppression were found to be nylon wool column adherent T cells and inhibited T cell dependent cytotoxicity rather than non-T cell dependent cytostasis. In vivo cell transfer experiments demonstrated that intravenous injection of suppressor cells to a host already inoculated with tumor cells mixed with antitumor effector cells resulted in significant enhancement of tumor growth. This inhibition of in vivo neutralization assay be suppressor cells was found in not only allogeneic but also syngeneic tumor system. Splenectomy at the time of tumor resection endowed the host with stronger resistance against subsequent reinoculated tumor than sham-splenectomy did, reflected by prolonged survival times. These results suggest that splenectomy combined with surgical removal of the tumor is a useful treatment of clinical malignancies.</p>
Keywords suppressor T cell nylon wool columu fractionation tumor enhancement splenectomy tumor-bearing mice.
Amo Type Article
Published Date 1981-10
Publication Title Acta Medica Okayama
Volume volume35
Issue issue4
Publisher Okayama University Medical School
Start Page 247
End Page 261
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457512
Web of Sience KeyUT A1981MK84200003
JaLCDOI 10.18926/AMO/31250
FullText URL fulltext.pdf
Author Ohmori, Masaki|
Abstract <p> The present status and the history of inbred strains of mice in the Experimental Animal Center, Okayama University Medical School, otherwise known as “The Colony of Okayama University Medical School” are described.</p>
Keywords inbred strain mouse colocy Okayama University Medical School.
Amo Type Article
Published Date 1981-10
Publication Title Acta Medica Okayama
Volume volume35
Issue issue4
Publisher Okayama University Medical School
Start Page 293
End Page 306
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457517
Web of Sience KeyUT A1981MK84200009
JaLCDOI 10.18926/AMO/31249
FullText URL fulltext.pdf
Author Kurose, Masao|
Abstract <p>Marked IgE-mediated histamine release from rat mast cells sensitized in vitro with mouse antiserum occurs in the presence of added Ca++ and phosphatidylserine (PS), although a considerable degree of antigen-induced histamine release which may utilize intracellular or cell-bound calcium is also observed. The decay in the responsiveness to Ca++ of the sensitized cells stimulated by antigen in Ca++-free medium in the presence of PS is relatively slow, and maximum release is produced by Ca++ added 1 min after antigen. Histamine release also occurs when Ca++ is added after PS in the absence of antigen to the sensitized cells suspended in Ca++-free medium. Unlike the antigen-induced release, the intensity of this non-antigen-induced release varies depending on both mast-cell and antiserum pools. A heat-labile factor(s), which is different from antigen-specific IgE antibody and is also contained in normal mouse serum, is involved in this reaction. In the antigen-nondependent (PS + Ca++)-induced release, no decay in the responsiveness to Ca++ is observed after PS addition. Both the antigen-induced and non-antigen-induced release are completed fairly rapidly and are dependent of temperature, pH and energy.</p>
Keywords histamine release rat peritoneal mast cells mouse lgE phosphatidylserine.
Amo Type Article
Published Date 1981-10
Publication Title Acta Medica Okayama
Volume volume35
Issue issue4
Publisher Okayama University Medical School
Start Page 235
End Page 245
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6170212
Web of Science KeyUT A1981MK84200002
JaLCDOI 10.18926/AMO/31248
FullText URL fulltext.pdf
Author Miyamoto, Kanji| Hayashi, Kyoichi| Tsubota, Teruhiko| Tanaka, Toshio|
Abstract <p>Chromosome analysis was performed on cells from a patient of null cell lymphoma, well-differentiated type. A 14q12 translocation was observed in all the banded cells. In addition, there were multiple chromosome abnormalities. This case will be useful in considering the significance of the 14q1(1-3) translocation in malignant lymphoma disease.</p>
Keywords malignant lymphoma chromosome analysis 14q12 translocation.
Amo Type Brief Note
Published Date 1981-10
Publication Title Acta Medica Okayama
Volume volume35
Issue issue4
Publisher Okayama University Medical School
Start Page 285
End Page 287
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457516
Web of Science KeyUT A1981MK84200007
JaLCDOI 10.18926/AMO/31247
FullText URL fulltext.pdf
Author Kaneyuki, Takao| Shohmori, Toshikiyo|
Abstract Diencephalon testosterone 5 alpha-reductase activity and plasma testosterone level were measured in the rat with liver injury caused by i.p. administration of CCl4. Testosterone levels in plasma were decreased after CCl4 injection in both group I (0.1ml/150g B.W./once a day for 2 days) and group II (0.01ml/150g B.W./twice a week for three weeks). 5 alpha-Reductase activity in the diencephalon was decreased both on the 7th day after administration in group I and in group II, while being transiently increased on the 1st day after administration in group I. These results suggest that testosterone metabolism in the brain is inhibited during liver disorders.
Keywords liver injury CCI<sub>4</sub> testosterone 5α-reductase testosterone estradiol-17β
Amo Type Brief Note
Published Date 1981-10
Publication Title Acta Medica Okayama
Volume volume35
Issue issue4
Publisher Okayama University Medical School
Start Page 279
End Page 284
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457515
Web of Science KeyUT A1981MK84200006