The effect of cytochalasin B (CB) on the surface structure of Ehrlich ascites tumor cells was investigated using the scanning electron microscope. The effect occurs in two steps: formation of zeiotic knobs on the cell surface and subsequent grouping of the knobs at one pole of the cell. The early step of zeiotic knob formation occurs at low concentrations of CB (0.5-1 microgram/ml) at 37 degrees C and at high concentrations of the drug (5-10 microgram/ml) at low temperature but within 1 min at 37 degrees C. This step is only partially inhibited by 5 x 10(-3) M sodium azide. The subsequent grouping of zeiotic knobs lasts for more than 2 min at 37 degrees C and occurs only in the case of high concentrations of CB. It is inhibited by sodium azide and is often associated with grouping of the microvilli, which are then lost from all of the cell surface except the area of knob-grouping.
en-copyright= kn-copyright= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ImanakaMasaaki en-aut-sei=Imanaka en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OtsukaNagayasu en-aut-sei=Otsuka en-aut-mei=Nagayasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakamotoShu en-aut-sei=Nakamoto en-aut-mei=Shu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MoriMasaharu en-aut-sei=Mori en-aut-mei=Masaharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=cytochalasin B kn-keyword=cytochalasin B en-keyword=Ehrlich ascites tumor cells kn-keyword=Ehrlich ascites tumor cells en-keyword=zeiosis kn-keyword=zeiosis en-keyword= scanning electron microscopy. kn-keyword= scanning electron microscopy. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=3 article-no= start-page=155 end-page=164 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Increased insulin binding to erythrocytes in chronic liver disease.Increased insulin binding to erythrocytes in chronic liver disease. en-subtitle= kn-subtitle= en-abstract= kn-abstract=125I-labeled insulin binding to peripheral human erythrocytes was studied in patients with chronic liver disease. The maximum specific 125I-labeled insulin binding was 12.10 +/- 1.13 %/4 x 10(9) cells (mean +/- SD, n = 10) in normal subjects, and significantly higher in cirrhotic patients (15.32 +/- 1.73 %, n = 11, P less than 0.01) but not in patients with acute and chronic hepatitis (11.44 +/- 2.10 %, n = 3 and 13.2 +/- 1.87 %, n = 7 respectively). The complication of diabetes mellitus significantly increased (P less than 0.05) the maximum insulin binding in chronic hepatitis. Scatchard analysis and average affinity analysis of the binding data suggest that increased insulin binding in cirrhotic patients is due to an increase in the number of insulin binding sites per erythrocytes. The complication of diabetes in chronic liver diseases results in an increase in affinity of insulin binding sites.
en-copyright= kn-copyright= en-aut-name=OkadaYoshio en-aut-sei=Okada en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=insulin binding kn-keyword=insulin binding en-keyword=erythrocyte kn-keyword=erythrocyte en-keyword= liver disease. kn-keyword= liver disease. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=3 article-no= start-page=179 end-page=195 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Pharmacological studies of 3-(3-methyl-3-butenyl)-1,2,3,4,5,6-hexahydro-6,11-dimethyl-8-hydroxy-2,6-methano-3-benzazocine. en-subtitle= kn-subtitle= en-abstract= kn-abstract=3-(3-methyl-3-butenyl)-1,2,3,4,5,6-hexahydro-6, 11-dimethyl-8-hydroxy-2,6-methano-3-benzazocine (KF-1820) is a derivative of benzomorphan and is different from pentazocine only in the site of the double bond. KF-1820 showed potent analgesic activity in all tests performed. KF-1820 was 6 to 12 times and 30 to 40 times more potent than morphine and pentazocine, respectively, when administered subcutaneously. KF-1820 had little or no narcotic antagonist property. Physical dependence liability tests indicated that KF-1820 may be a little less, or as liable as, pentazocine to produce physical dependence. ID50 values of KF-1820, pentazocine and morphine for depression of contractions of isolated guinea pig ileum to coaxial stimulation correlated well with their analgesic activities in the rodent. The dissociation equilibrium constant of KP-1820 confirmed the in vivo finding that KF-1820 had little or no narcotic antagonist property.
en-copyright= kn-copyright= en-aut-name=NakamuraNobuo en-aut-sei=Nakamura en-aut-mei=Nobuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=KF-1820 kn-keyword=KF-1820 en-keyword=analgesic kn-keyword=analgesic en-keyword=non-narcotic antagonism kn-keyword=non-narcotic antagonism en-keyword=dependence kn-keyword=dependence en-keyword= guinea pig ileum. kn-keyword= guinea pig ileum. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=3 article-no= start-page=173 end-page=178 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Generation of superoxide and hydrogen peroxide during interaction of nitrite with human hemoglobin. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Generation of superoxide and hydrogen peroxide during interaction of nitrite with human hemoglobin was detected by chemiluminescence of luminol. Luminol chemiluminescence was inhibited by the addition of superoxide dismutase (SOD) and catalase. Methemoglobin formation induced by nitrite was also inhibited by the addition of SOD and catalase. The mechanism of methemoglobin formation by nitrite was discussed in regard to the oxidation of hemoglobin by superoxide and hydrogen peroxide as generated by the interaction of nitrite with hemoglobin.
en-copyright= kn-copyright= en-aut-name=WatanabeShinsaku en-aut-sei=Watanabe en-aut-mei=Shinsaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=nitrite kn-keyword=nitrite en-keyword=chemiluminescence kn-keyword=chemiluminescence en-keyword=methemoglobin kn-keyword=methemoglobin en-keyword= superoxide dismutase. kn-keyword= superoxide dismutase. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=3 article-no= start-page=165 end-page=172 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A new adrenal computer imaging technique using dual-radioisotopes. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Computer processed adrenal imaging using dual-radioisotopes, 6 beta-iodomethyl-19-nor-cholest-5(10)-en-3 beta-ol-131I and 99mTc-phytate was performed in 12 patients with primary aldosteronism and 4 with Cushing's syndrome due to adrenocortical tumor. Adreno-photoscanning and hepato-photoscanning were performed in the same position 2-4 days following intravenous administration of radiocholesterol. The scintigraphic information was stored on cassettes and scan subtraction and a digital-computer method for data smoothing were performed on an oscilloscope. The tumor site could be determined in all cases until day 4 by this computer processed image.
en-copyright= kn-copyright= en-aut-name=OhashiTeruhisa en-aut-sei=Ohashi en-aut-mei=Teruhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MoriokaMassaki en-aut-sei=Morioka en-aut-mei=Massaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MitsuhataNaoki en-aut-sei=Mitsuhata en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AkaedaTeruaki en-aut-sei=Akaeda en-aut-mei=Teruaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhmoriHiroyuki en-aut-sei=Ohmori en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=new adrenal imaging technique kn-keyword=new adrenal imaging technique en-keyword=dual radioisotopes kn-keyword=dual radioisotopes en-keyword= adrenocortical tumor. kn-keyword= adrenocortical tumor. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=3 article-no= start-page=215 end-page=219 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Demonstration of dnp groups on the draining lymph node cells of guinea pigs following skin painting with DNCB by peroxidase labelled antibody method. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The distribution of 2,4-dinitrophenyl (DNP) groups in the draining lymph nodes of guinea pigs 12 h after painting the skin with 2,4-dinitrochlorobenzene (DNCB) was examined by a peroxidase labelled antibody method using antibody against DNP groups. DNP groups were detected on cells that were found mainly in the subcapsular sinus of the lymph nodes. Electron microscopic examination showed DNP groups distributed on the surface of lymphocytes. The significance of these findings is discussed.
en-copyright= kn-copyright= en-aut-name=TadaHiroshi en-aut-sei=Tada en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakagawaToshifumi en-aut-sei=Nakagawa en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakaiwaTakashi en-aut-sei=Takaiwa en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakagawaShojiro en-aut-sei=Nakagawa en-aut-mei=Shojiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Kawasaki Medical School en-keyword=contact sensitivity kn-keyword=contact sensitivity en-keyword=2 kn-keyword=2 en-keyword=4-dinitrochlorobenzene kn-keyword=4-dinitrochlorobenzene en-keyword=dinitrophenyl group kn-keyword=dinitrophenyl group en-keyword= peroxidase. kn-keyword= peroxidase. END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=3 article-no= start-page=205 end-page=213 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Pulsatile arginine vasopressin release from the rat hypothalamo neurohypophyseal system during osmotic stimulation. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Arginine vasopressin (AVP) was released in vitro in a pulsatile pattern from the hypothalamo-neurohypophyseal system (HNS) and from the hypothalamus during continuous hyperosmotic stimuli with NaCl or fructose. No significant difference was found in the AVP pulse frequency between the two kinds of hyperosmotic agents. AVP was released from the HNS in a dose-related manner under NaCl stimulation. When the neural lobe was stimulated with NaCl or fructose, a clear AVP pulse pattern was not apparent. Urea failed to evoke a significant AVP release from the neural lobe or HNS. A stepwise increase in NaCl stimulation from 5 to 25 mEq induced a AVP response from the HNS and hypothalamus similar to that under constant stimulation at 25 mEq NaCl. This phenomenon was also found with fructose or sucrose. These results suggest that AVP release from the HNS during continuous osmotic stimulation has a pulsatile pattern regardless of the hyperosmotic substance or osmotic pressure. This AVP release accurately reflects the physiological function of the hypothalamus without modulation in the neural lobe. These results also suggest that the total amount of AVP was related to the osmotic pressure or the osmotic substance but that the frequency of the pulse release was not, moreover, that the AVP release depends not only on the absolute osmotic pressure, but also on the changing rate of osmotic pressure.
en-copyright= kn-copyright= en-aut-name=OhnoNorihito en-aut-sei=Ohno en-aut-mei=Norihito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=rginine vasopressin kn-keyword=rginine vasopressin en-keyword=hypothalamo-neurohypophyseal system kn-keyword=hypothalamo-neurohypophyseal system en-keyword=neural lobe kn-keyword=neural lobe en-keyword=hypothalamus kn-keyword=hypothalamus en-keyword= perifusion method. kn-keyword= perifusion method. END