JaLCDOI 10.18926/AMO/31288
FullText URL fulltext.pdf
Author Sasaki, Junzo| Imanaka, Masaaki| Watanabe, Sadahiro| Otsuka, Nagayasu| Nakamoto, Shu| Mori, Masaharu|
Abstract <p>The effect of cytochalasin B (CB) on the surface structure of Ehrlich ascites tumor cells was investigated using the scanning electron microscope. The effect occurs in two steps: formation of zeiotic knobs on the cell surface and subsequent grouping of the knobs at one pole of the cell. The early step of zeiotic knob formation occurs at low concentrations of CB (0.5-1 microgram/ml) at 37 degrees C and at high concentrations of the drug (5-10 microgram/ml) at low temperature but within 1 min at 37 degrees C. This step is only partially inhibited by 5 x 10(-3) M sodium azide. The subsequent grouping of zeiotic knobs lasts for more than 2 min at 37 degrees C and occurs only in the case of high concentrations of CB. It is inhibited by sodium azide and is often associated with grouping of the microvilli, which are then lost from all of the cell surface except the area of knob-grouping.</p>
Keywords cytochalasin B Ehrlich ascites tumor cells zeiosis scanning electron microscopy.
Amo Type Article
Published Date 1981-06
Publication Title Acta Medica Okayama
Volume volume35
Issue issue3
Publisher Okayama University Medical School
Start Page 197
End Page 204
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457509
Web of Sience KeyUT A1981MD16600005
JaLCDOI 10.18926/AMO/31287
FullText URL fulltext.pdf
Author Okada, Yoshio|
Abstract <p>125I-labeled insulin binding to peripheral human erythrocytes was studied in patients with chronic liver disease. The maximum specific 125I-labeled insulin binding was 12.10 +/- 1.13 %/4 x 10(9) cells (mean +/- SD, n = 10) in normal subjects, and significantly higher in cirrhotic patients (15.32 +/- 1.73 %, n = 11, P less than 0.01) but not in patients with acute and chronic hepatitis (11.44 +/- 2.10 %, n = 3 and 13.2 +/- 1.87 %, n = 7 respectively). The complication of diabetes mellitus significantly increased (P less than 0.05) the maximum insulin binding in chronic hepatitis. Scatchard analysis and average affinity analysis of the binding data suggest that increased insulin binding in cirrhotic patients is due to an increase in the number of insulin binding sites per erythrocytes. The complication of diabetes in chronic liver diseases results in an increase in affinity of insulin binding sites.</p>
Keywords insulin binding erythrocyte liver disease.
Amo Type Article
Published Date 1981-06
Publication Title Acta Medica Okayama
Volume volume35
Issue issue3
Publisher Okayama University Medical School
Start Page 155
End Page 164
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457506
Web of Sience KeyUT A1981MD16600001
JaLCDOI 10.18926/AMO/31286
FullText URL fulltext.pdf
Author Nakamura, Nobuo|
Abstract <p>3-(3-methyl-3-butenyl)-1,2,3,4,5,6-hexahydro-6, 11-dimethyl-8-hydroxy-2,6-methano-3-benzazocine (KF-1820) is a derivative of benzomorphan and is different from pentazocine only in the site of the double bond. KF-1820 showed potent analgesic activity in all tests performed. KF-1820 was 6 to 12 times and 30 to 40 times more potent than morphine and pentazocine, respectively, when administered subcutaneously. KF-1820 had little or no narcotic antagonist property. Physical dependence liability tests indicated that KF-1820 may be a little less, or as liable as, pentazocine to produce physical dependence. ID50 values of KF-1820, pentazocine and morphine for depression of contractions of isolated guinea pig ileum to coaxial stimulation correlated well with their analgesic activities in the rodent. The dissociation equilibrium constant of KP-1820 confirmed the in vivo finding that KF-1820 had little or no narcotic antagonist property.</p>
Keywords KF-1820 analgesic non-narcotic antagonism dependence guinea pig ileum.
Amo Type Article
Published Date 1981-06
Publication Title Acta Medica Okayama
Volume volume35
Issue issue3
Publisher Okayama University Medical School
Start Page 179
End Page 195
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457508
Web of Sience KeyUT A1981MD16600004
JaLCDOI 10.18926/AMO/31285
FullText URL fulltext.pdf
Author Watanabe, Shinsaku| Ogata, Masana|
Abstract <p>Generation of superoxide and hydrogen peroxide during interaction of nitrite with human hemoglobin was detected by chemiluminescence of luminol. Luminol chemiluminescence was inhibited by the addition of superoxide dismutase (SOD) and catalase. Methemoglobin formation induced by nitrite was also inhibited by the addition of SOD and catalase. The mechanism of methemoglobin formation by nitrite was discussed in regard to the oxidation of hemoglobin by superoxide and hydrogen peroxide as generated by the interaction of nitrite with hemoglobin.</p>
Keywords nitrite chemiluminescence methemoglobin superoxide dismutase.
Amo Type Article
Published Date 1981-06
Publication Title Acta Medica Okayama
Volume volume35
Issue issue3
Publisher Okayama University Medical School
Start Page 173
End Page 178
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6270983
Web of Science KeyUT A1981MD16600003
JaLCDOI 10.18926/AMO/31284
FullText URL fulltext.pdf
Author Ohashi, Teruhisa| Morioka, Massaki| Mitsuhata, Naoki| Akaeda, Teruaki| Ohmori, Hiroyuki|
Abstract <p>Computer processed adrenal imaging using dual-radioisotopes, 6 beta-iodomethyl-19-nor-cholest-5(10)-en-3 beta-ol-131I and 99mTc-phytate was performed in 12 patients with primary aldosteronism and 4 with Cushing's syndrome due to adrenocortical tumor. Adreno-photoscanning and hepato-photoscanning were performed in the same position 2-4 days following intravenous administration of radiocholesterol. The scintigraphic information was stored on cassettes and scan subtraction and a digital-computer method for data smoothing were performed on an oscilloscope. The tumor site could be determined in all cases until day 4 by this computer processed image.</p>
Keywords new adrenal imaging technique dual radioisotopes adrenocortical tumor.
Amo Type Article
Published Date 1981-06
Publication Title Acta Medica Okayama
Volume volume35
Issue issue3
Publisher Okayama University Medical School
Start Page 165
End Page 172
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457507
Web of Science KeyUT A1981MD16600002
JaLCDOI 10.18926/AMO/31283
FullText URL fulltext.pdf
Author Tada, Hiroshi| Nakagawa, Toshifumi| Takaiwa, Takashi| Nakagawa, Shojiro|
Abstract <p>The distribution of 2,4-dinitrophenyl (DNP) groups in the draining lymph nodes of guinea pigs 12 h after painting the skin with 2,4-dinitrochlorobenzene (DNCB) was examined by a peroxidase labelled antibody method using antibody against DNP groups. DNP groups were detected on cells that were found mainly in the subcapsular sinus of the lymph nodes. Electron microscopic examination showed DNP groups distributed on the surface of lymphocytes. The significance of these findings is discussed.</p>
Keywords contact sensitivity 2 4-dinitrochlorobenzene dinitrophenyl group peroxidase.
Amo Type Brief Note
Published Date 1981-06
Publication Title Acta Medica Okayama
Volume volume35
Issue issue3
Publisher Okayama University Medical School
Start Page 215
End Page 219
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457511
Web of Science KeyUT A1981MD16600007
JaLCDOI 10.18926/AMO/31282
FullText URL fulltext.pdf
Author Ohno, Norihito|
Abstract <p>Arginine vasopressin (AVP) was released in vitro in a pulsatile pattern from the hypothalamo-neurohypophyseal system (HNS) and from the hypothalamus during continuous hyperosmotic stimuli with NaCl or fructose. No significant difference was found in the AVP pulse frequency between the two kinds of hyperosmotic agents. AVP was released from the HNS in a dose-related manner under NaCl stimulation. When the neural lobe was stimulated with NaCl or fructose, a clear AVP pulse pattern was not apparent. Urea failed to evoke a significant AVP release from the neural lobe or HNS. A stepwise increase in NaCl stimulation from 5 to 25 mEq induced a AVP response from the HNS and hypothalamus similar to that under constant stimulation at 25 mEq NaCl. This phenomenon was also found with fructose or sucrose. These results suggest that AVP release from the HNS during continuous osmotic stimulation has a pulsatile pattern regardless of the hyperosmotic substance or osmotic pressure. This AVP release accurately reflects the physiological function of the hypothalamus without modulation in the neural lobe. These results also suggest that the total amount of AVP was related to the osmotic pressure or the osmotic substance but that the frequency of the pulse release was not, moreover, that the AVP release depends not only on the absolute osmotic pressure, but also on the changing rate of osmotic pressure.</p>
Keywords rginine vasopressin hypothalamo-neurohypophyseal system neural lobe hypothalamus perifusion method.
Amo Type Article
Published Date 1981-06
Publication Title Acta Medica Okayama
Volume volume35
Issue issue3
Publisher Okayama University Medical School
Start Page 205
End Page 213
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6457510
Web of Science KeyUT A1981MD16600006