ID | 32054 |
JaLCDOI | |
FullText URL | |
Author |
Misumi, Hiromasa
Oda, Takuzo
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Abstract | RNA polymerase was extracted from the Schmidt-Ruppin strain of Rous sarcoma virus (SR-RSV)-induced C3H/He mouse ascites sarcoma cells (SR-C3H). RNA polymerase was separated into RNA polymerases I and II by DEAE-Sephadex chromatography. RNA polymerase I was separated into Ia and Ib fractions by phospho-cellulose chromatography. In SR-C3H cells RNA polymerase Ib was the main component of RNA polymerase I. At 0.05--0.1 M ammonium sulphate RNA polymerase I transcribed native DNA most actively, and RNA polymerase II transcribed denatured DNA most actively. Partial digestion of DNA by DNAase I enhanced RNA synthesis by RNA polymerases I and II. At ionic strength over 0.2 M ammonium sulphate, the initiation reaction of RNA polymerases I and II was inhibited. The initiation complexes of RNA polymerases I and II with native DNA were more stable against high salt concentration than with denatured DNA. |
Keywords | RNA polymerase
Pous sarcoma virus
mouse ascites sarcoma
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 1979-04
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Volume | volume33
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Issue | issue2
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Publisher | Okayama University Medical School
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Start Page | 91
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End Page | 102
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
NAID |