ID | 32053 |
JaLCDOI | |
FullText URL | |
Author |
Hashimoto, Kozo
Yunoki, Sho
Hosogi, Hidemi
Takahara, Jiro
Ofuji, Tadashi
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Abstract | Biologically active peptides and neurotransmitter substances were added to anterior pituitary cell cultures to examine the presence of corticotropin releasing factor (CRF)-like activity. Hypothalamic extract (HE) induced significant dose-related increase of ACTH, and the lowest effective dose was 0.01 HE/ml. Other tested substances including luteinizing hormone-releasing hormone, thyrotropin releasing hormone, melanocyte stimulating hormone release inhibiting factor, somatostatin, substance P, neurotensin, beta-endorphin. leu-enkephalin, met-enkephalin, bradykinin, norepinephrine, dopamine, serotonin, acetylcholine, histamine, gamma-amino butyric acid or gamma-hydroxy butyric acid showed no CRF-like activity. Relatively high doses of lysine vasopressin, arginine vasopressin and angiotensin II increased the release of ACTH in pituitary cell cultures, but the maximal ACTH response was markedly less than with HE. These results indicate that cultured anterior pituitary cells are sensitive and fairly specific in detecting CRF(s) comparing with other detecting procedures. |
Keywords | pituitary cell cultures
corticotropin releasing factor
ACTH
neuropeptides
neurotransmitter substances
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 1979-04
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Volume | volume33
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Issue | issue2
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Publisher | Okayama University Medical School
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Start Page | 81
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End Page | 90
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
NAID |