JaLCDOI 10.18926/AMO/32752
FullText URL fulltext.pdf
Author Kimoto, Tetsuo| Grace, James T.|
Abstract

The present report describes the findings on the infectivity of DNA partially purified from SV-40 which was propagated in the monkey kidney cells (BSC-1) in vitro and the importance of nucleic acids as oncogenic factors, particularly the induction of tumor by DNA in newborn hamsters. 593 newborn hamsters in total were used in the present experiments, and cannibalism among them posed as a serious problem. On 30 days postinoculation, very remarkable changes occurred in the liver, lung and subcutaneous areas. Cellular responses of the perivascular cells were predominant. and they were distributed in the interstitial tissues of the liver (liver cirrhosis in primates) and lung. Three hamsters of those subcutaneously inoculated with nucleic acids developed tumors and two tumors appeared in the subcutaneous tissues on 130 days postinoculation, which were identified to be the ones induced by intact SV-40 virus. Other tumors appeared in the liver, lung, intestinal ducts and abdominal surface at 126 days after subcutaneous injection. The cytological observations revealed multiple hemangiosarcoma combined with proliferation of the perivascular cells. On the other hand, cellular responses to nucleic acids were more marked by inoculation of the cell-free filtrate of BSC-1 infected by DNA than of DNA, and essential histologic findings were similar to the respo.nse to infectious DNA. Thirty-nine hamsters (30 per cent) developed tumor within about 200 days postinoculation of the filtrates. Sarcomas were common and they were confined to the subcutaneous tissues in 35 hamsters and to the peritoneum in two others by subcutaneous inoculation of the filtrates. The intestinal gland-cell carcinomas, however, could be induced at 37 and 59 days postinoculation in two hamsters of one litter (7 newborn hamsters) and in the other three newborn hamsters subcutaneous sarcomas were induced by inoculation of the same agent. These results suggest that the observation on the oncogenic capacity of nucleic acids would give us a clue to resolve the course of cancer from the view point of the infectious nucleic acid.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1966-02
Volume volume20
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 27
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 4288540
NAID 120002312229
JaLCDOI 10.18926/AMO/32751
FullText URL fulltext.pdf
Author Seito, Takashi| Ogata, Takuro| Nobuto, Hideo| Kawashima, Takao|
Abstract

The distribution and activities of five hydrolytic and eight oxidative enzymes were histochemically studied in 60 different tumors of the human intestines. Benign polyp showed similar activities of most enzymes as those in normal crypt cells of large intestine with exception of higher activity of succinic dehydrogenase in benign polyp than in crypt cells. Malignant polyp had higher activities of most oxidative enzymes. Reticulo-sarcoma had weak activities of all enzymes. Carcinoid had strong activities of glucose-6-phosphate dehydrogenase and isocitric dehydrogenase while very weak of succinic dehydrogenase. Carcinoma showed varying degrees of the activity of all enzymes. Alkaline phosphatase and aminopeptidase were almost negative in all cells but in the stromal elements their weak activities were sporadically observed. Most enzymes were decreased in the central area of the carcinoma cell nestle, while in the infiltrating area or in the margin of cell nestle they were not decreased and sometimes increased.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1966-02
Volume volume20
Issue issue1
Publisher Okayama University Medical School
Start Page 37
End Page 52
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 4224241
NAID 120002311960
JaLCDOI 10.18926/AMO/32750
FullText URL fulltext.pdf
Author Shinzeki, Ken| Narusue, Mitsuo| Yamamoto, Yasuhisa|
Abstract

1. The ratios of free 5α-cholestan-3β-ol and cholesterol and esterified 5α-cholestan-3β-ol were higher in pylorus than in cardia. 2. Esterified cholesterol level was higher in cardia than in pylorus. 3. Among the stomach cancer tissues examined free cholesterol level was higher than in the non-cancerous. 4. Esterified 5α-cholestan-3β-o1 and cholesterol levels were lower in the cancerous tissues than in the non cancerous.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1966-02
Volume volume20
Issue issue1
Publisher Okayama University Medical School
Start Page 29
End Page 36
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 4224240
NAID 120002311428