Acta Medica Okayama volume15 issue5

Neomyographic examinations were made by the authors on 28 patients. The extent of reconvalescence was measured on the basis of changes in the values recorded by the myograms taken before and after the treatment.

Every cytologist in biology or medicine knows the so-called "Golgi-Complex", but no cytologist can state exactly the structure and the function of this complex. Nevertheless, in the last six years this" Golgi complex" in about 100 different cells has been seen in the electron microscope. That is the reason I have tried to make a comparative study of these fields. I would like to give a short review of this investigation here, having been kindly invited by Prof. S. SENO.

Attempts were made to identify menstrual blood by means of paper electrophoresis with preparation of extracts of menstrual blood isolated under various conditions and mixed with human fibrin. Also similar analyses were conducted with blood aspirated from the median cubital vein of a woman during menstruation as well as from a man as the control, also with extracts of lochial blood from a woman after normal delivery, and of the blood obtained at arrtificial abortion. Animal fibrins (from rabbit, mouse, steer, and guinea pig) were also used to see the lytic action of the bloods. The following are the results of the present experiments. 1. The identification of menstrual blood by means of paper electrophoresis is a simple method in legal medicine and its electrophorogram is an excellent method to offer an evidence of proof for mentrual blood. 2. By this method it is possible to identify the menstrual bloodstain even after the lapse of time as much as 6 months. 3. It is possible to identify even putrefied menstrual bloodstain. 4. In the case where the material stained with menstrual blood is found in water, it is not possible to identify the menstrual blood by this method. 5. When the menstrual blood is heated at 60°C over 30 minutes, it becomes impossible to identify it by this method. 6. In the case of venous blood during menstruation fibrinolytic product can be detected only on the first day of menstruation, but since it appears only in trace, it is easy to differentiate it from menstrual blood. 7. As for lochial blood the fibrinolytic product can be detected only in the blood obtained on the first and second days of puerperium, but the amount being so slight that it can readily be distinguished from menstrual blood. 8. In the case of the blood obtained at artificial abortion fibrinolytic product appears just as much as in the case of menstrual blood, and thus it is impossible to differentiate it from mentrual blood by this method. 9. As for the use of human fibrin it is best to employ it while it isdresh, but the human fibrin up to 6 days old can be used. However, the older is the human fibrin the lesser the fibrinolytic product detectable. 10. In the case using animal fibrins mixed with the extract of menstrual blood some do produce fibrinolytic product in trace, but since there is a danger of also producing the fibrinolytic product-like substance in venous blood, it is advisable not to use animal fibrins.

On avait execute 160 mesurages des 40 serums des donneurs du sang (20 hommes, 20 femmes). La moitie des mesurages etait faits a l'aide de plasma oxalique, l'autre moitie le servit du substrat constituant plasma sec dilue (7.5 : 200.0). Les resultats des premiers mesurages etaient superieurs aux valeurs des resultats obtenus dans l'autre groupe des recherches. La difference etait causee par une plus forte concentration de la thrombine dans le melange d'incubation et d'inactivation teste contre le plasma sec dilue. Les recherches accessoires ont demontre que les memes concentrations de la thrombine coagulent plus vite le plasma oxalique, que plasma sec dilue, et que pour obtenir la meme valeur du temps de coagulation avec les deux substrats il faut appliquer plus de thrombine pour coaguler plasma sec dilue en reaction controle visant la preparation de la solution-mere et de la solution-modele de la thrombine. On avait constate aussi des differences essentielles en inactivation entre les hommes et les femmes. Chez les hommes les donnees d'inactivation sont superisures que chez les femmes. Ces differences etaient moins accentuees aux premieres, qu'aux les suivantes minutes d'incubation. Ainsi on doit comparer les resultats des examens toujours avec la moyenne du meme sexe, que la personne examinee, et non avec la moyenne des deux sexes.

The human tumor H. Ep. #3 maintained in rats could be transferred for 1-8 generations in treated guinea pigs. H. Ep. #3 grew in the subcutaneous and intramuscular sites in each host at the same time. The treatment with the combination of X-ray 250 r. and 80 mg/kg of cortisone turned out to be the optimal conditioning studied. The number of tumor takes averaged 95.7-100 per cent in the subcutaneous site in guinea pigs treated with optimal conditioning, but in the intramuscular site, the number of tumor takes was 65.2-93.8 per cent. Host mortality varied from 4.2-37.5 per cent in the hosts treated with optimal conditioning. The subcutaneous tumor weights in hosts treated with optimal conditioning averaged 3.3 gm, and their intramuscular tumor weights averaged 5.6-6.2 gm. Tumor weights in hosts treated with only cortisone averaged 1-2 gm in both subcutaneous and intramuscular sites. Histological findings. for the original tumors were found to be the same as that for the successful transplanted tumors in the guinea pigs. The malignancy of the tumor was evaluated by the criteria of anaplasia, invasion, rapidity of growth, and ease of maintenance of transplants. There was no metastasis found in any organs.

Through the use of an automatic photo tube dew-point hygrometer, the author succeeded in measuring dew point of gas flows continuously in anesthetic circuits. Simultaneous thermometries were done on the nasal or oral mucosa, on the respiratory gas flows in the anesthetic mask or the endotracheal tube, and on the gas in the inhaling conduit. Experiments were performed on ten adults patients undergoing various types of surgery under general inhalation anesthesia. Anesthetic technics were varied intentionally during the measurements. Thus, both absolute and relative humidities of exhaled and inhaled gases, and respiratory water and heat losses were calculated under various anesthetic conditions, and physiological and clinical considerations were discussed. The conclusions obtained from this research are as follows: (1) When a non-rebreathing system is applied, moisture content of exhaled gas is minimal, and respiratory losses of both water and heat are maximum. With a semi-closed circle method, according to decreasing fresh gas flows, the humidity of the inspiratory and expiratory gases becomes higher, and both heat and water losses through respiration are lessened. When a closed circle method, with carbon dioxide absorption, is employed, temperature and humidity of gas in the inhaling conduit are highest, and the expired gas offers the maximum temperature and moisture contenL Both water and heat losses from anesthesia become minimal when administered in a closed system. (2) While the water and heat that a patient loses through respiration increase with increasing breathing capacity, they are still small parts of the total water and heat losses of the patient. Water and heat losses via anesthesia systems are not so predominant in maintaining water balance and heat regulation of patients during anesthesia and surgery.