JaLCDOI 10.18926/AMO/31246
FullText URL fulltext.pdf
Author Utsumi, Kozo|
Abstract

Some critical experiments have been carried out on the microspectrophotometry using the lymphocytes of a mouse, stained with Feulgen reaction, revealing that most reliable value can be attained by illuminating the material with a small spot-light and integrating the area surrounded by the extinction curve drawn by tracing along the diameter of the smeared and fixed cell.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1959-04
Volume volume13
Issue issue1
Publisher Okayama University Medical School
Start Page 45
End Page 56
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
NAID 120002313004
JaLCDOI 10.18926/AMO/31245
FullText URL fulltext.pdf
Author Szirmai, Endre|
Abstract

Der Verfasser macht die HARTERT sche Thrombelastographie bekannt; das Verfahren ist noch nicht in Ungarn und vielen anderen Laendern im Gebrauch. Auf Grunde seine Untersuchungen geht er auf die detaillierte Auswertung des Thrombelastograme ein und referiert ueber die Anwendung und Brauchbarkeit der Thrombelastographes und gibt im Tabelle 1. den Angaben verschiedenen Patienten bekannt.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1959-04
Volume volume13
Issue issue1
Publisher Okayama University Medical School
Start Page 71
End Page 82
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
NAID 120002313061
JaLCDOI 10.18926/AMO/31244
FullText URL fulltext.pdf
Author Murakami, Sakae| Oka, Yoshikazu| Matsuura, Yoshiyuki| Yoshioka, Tatsuji|
Abstract

After grinding the tubercle bacilli cells, both human virulent strain, H37Rv, and avirulent strain, H37Ra, cultured in 5auton's medium, and obtaining three fractions of R1, S1 and R2 (R1, the first sediment; S1, the second supernatant; and R2, the second sediment) by the ultracentrifugation, the authors studied the enzymatic activities and the antigenic capacity against infection of these fractions; and obtained the following results: 1) Although the R1-fraction confers the defensive forte to mice in some degree, because of the presence of living bacilli in the fraction, it is difficult to decide definitely whether the defensive force owes its capability to this fraction or to living bacilli at the present stage of our experiment. 2) The S1-fraction possesses enzymatic activity on various substrates, but it does not confer animal any defensive force against infection. 3) The R2-fraction specifically oxidizes lactate and succinate" and it can markedly impart animal the defensive ability against infection.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1959-04
Volume volume13
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 14
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
NAID 120002313008
JaLCDOI 10.18926/AMO/31243
FullText URL fulltext.pdf
Author Oda, Takuzo| Matsuoka, Kenichi| Okazaki, Hiroaki| Kawasaki, Masayoshi|
Abstract

1. Histochemical and cytochemical studies with respect to the sites of reaction were made on the succinic dehydrogenase system activity of human and animal tissues using ditetrazolium salts, namely, neotetrazolium chloride, nitro-neotetrazolium chloride, and nitra-blue tetrazolium chloride. 2. The advantages and disadvantages of each ditetrazolium salt for histochemical and cytochemical purposes and the reaction taking place in frozen tissue sections and that in fresh tissue blocks were compared, and the method of procedure suitable for each condition was established with some modification. 3. Selecting conditions suitable for cytochemical purpose, it was shown that the reaction took place at the sites coinciding with mitochondria, and the distribution of the enzyme reaction was also examined. In addition, several new findings in the brains and other tissues cytochemically made clear were pointed out.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1959-04
Volume volume13
Issue issue1
Publisher Okayama University Medical School
Start Page 31
End Page 44
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
NAID 120002312439
JaLCDOI 10.18926/AMO/31242
FullText URL fulltext.pdf
Author Okumura, Nikichi| Otsuki, Saburo| Fukai, Nobuhiro|
Abstract

The present paper describes each pattern of the free amino acids in different parts of the dog brain determined by ion-exchange chromatography. The parts examined have been the cerebral cortex, cerebral white matter, cerebellar hemisphere, cerebellar vermis, caudate nucleus, thalamus, hypothalamus, and medulla oblongata. Gamma-aminobutyric acid concentration was the highest in the hypothalamus. Glutamic acid showed lower values in the white matter, hypothalamus, and medulla oblongata. Aspartic acid showed lower values in the white matter and caudate nucleus and higher values in the medulla oblongata. Glutathione and cystathionine showed higher values in the thalamus. N-Acetylaspartic acid showed lower values in the white matter and medulla oblongata. Glycine and alanine showed higher values in the medulla oblongata.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1959-04
Volume volume13
Issue issue1
Publisher Okayama University Medical School
Start Page 27
End Page 30
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
NAID 120002313141
JaLCDOI 10.18926/AMO/31241
FullText URL fulltext.pdf
Author Murakami, Sakae| Yoshioka, Tatsuji| Oka, Yoshikazu| Matsuura, Yoshiyuki|
Abstract

By physically destroying typhoid bacilli and centrifuging at a high speed, an insoluble granular fraction (P1) and soluble fraction (S1) were obtained. Chemical and enzymologic properties of these substances as well as their influences on the protective ability against infection were studied; and the following results were attained: 1. P1 contains an extremely small amount of proteins when compared with S1. 2. The enzymologic activity of P1 is entirely different from that of S1. In P1 the respiratory enzyme system of only lactate and succinate is localized. 3. Although both P1 and S1 possess the antibody-producing ability in serum of rabbit to the same high degree, P1 imparts to mice a markedly high protective ability against infection. 4. By the heat-treatment of P1 its antigenicity is lost at the same time.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1959-04
Volume volume13
Issue issue1
Publisher Okayama University Medical School
Start Page 15
End Page 26
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
NAID 120002312808
JaLCDOI 10.18926/AMO/31240
FullText URL fulltext.pdf
Author Hiraki, Kiyoshi| Inoue, Masakatsu|
Abstract

From these results it is but natural to assume that the antigen-antibody reaction is involved in the phenomenon, eosinophilia. The antigen in this instance is the filtrate of hookworm emulsion, and the serum of hookworm disease as well as the bone marrow can be thought to contain the antibody. In any case, so long as the medium contains the serum or bone marrow or both of them obtained from the patient of hookworm disease, eosinophilia and the acceleration in the motility of eosinophils are brought about in the growth zone by addition of the filtrate of hookworm emulsion. Therfore, as for the mechanism inducing hookworm eosinophilia, it may by interpreted that the patient of hookworm disese is repeatedly sensitized by the antigen arising all probability from the metabolic products of hookworms or from the dead bodies of the worms; and producing the antibody in tissues and blood, thus the antigen-antibody reaction is elicited in vivo as long as hookworms live in the human body so that the increase in the mitosis and the acceration in the motility of eosinophils in the bone marrow are brought about with the resultant continuous discharge of a large quantity of eosinophils from the bone marrow parenchma into the sinusoids, there by inducing eosinophilia in the peripheral blood.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1959-04
Volume volume13
Issue issue1
Publisher Okayama University Medical School
Start Page 65
End Page 70
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
NAID 120002312931
JaLCDOI 10.18926/AMO/31239
FullText URL fulltext.pdf
Author Hiraki, Kiyoshi| Inoue, Masakatsu|
Abstract

Of eosinophilias that we often encounter clinically, we selected two of the most representative ones, namely, hookworm diseae and bronchial astma, for our present sternal bone-marrow tissue culture, and studied the movement patterns and wandering capacity of eosinophils. As the results, even in those eosinophils that show no significant change other than the increase in number in ordinary stained-smear specimens of peripheral blood or bone marrow, it has been clarified that, when observed under living condition, they reveal a picture specific to individualistic behaviors according to diseases. Therefore, it can be assumed that in the pathologic condition what is known as eosinopilia not only eosinophils increase in number but also qualitative changes of eosinophlils specific to each disease are brought about, and consequently these specific changes are reflected on the movement patterns of the eosinophil.

Amo Type Article
Publication Title Acta Medicinae Okayama
Published Date 1959-04
Volume volume13
Issue issue1
Publisher Okayama University Medical School
Start Page 57
End Page 64
NCID AA00041342
Content Type Journal Article
language English
File Version publisher
Refereed True
NAID 120002312682